LOCUS       RC202820                7227 bp    DNA     circular  SYN 
DEFINITION  VPS51 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 2 (C11orf2)
ACCESSION   NM_013265
VERSION     NM_013265.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3384..3475 
                     /label="Myc-DDK"  
     misc_feature    3505..4090 
                     /label="PolyA signal"  
     misc_feature    3562..3581 
                     /label="Restriction Enzyme"  
     misc_feature    4366..5038 
                     /label="ColE1"  
     misc_feature    5468..6262 
                     /label="Kan/Neo"  
     misc_feature    6297..6599 
                     /label="SV40 ori"  
     misc_feature    6685..7162 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3366..3372 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3366 
                     /label="VPS51"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCA GCTGCCGCCG GGCCTAGCCC GGGGTCTGGA CCTGGGGACT 
     1081 CCCCAGAAGG GCCCGAGGGG GAGGCTCCGG AGCGTCGGCG GAAGGCGCAC GGGATGCTGA 
     1141 AGCTTTACTA CGGCCTCTCG GAAGGGGAGG CGGCGGGACG CCCCGCGGGG CCCGACCCCC 
     1201 TGGACCCGAC TGATCTGAAC GGGGCGCACT TCGACCCGGA AGTTTACCTA GACAAGCTGC 
     1261 GTAGAGAGTG CCCTCTGGCC CAGTTGATGG ACAGTGAGAC GGACATGGTG CGGCAGATCC 
     1321 GGGCTCTAGA CAGCGACATG CAGACCCTGG TCTATGAGAA CTACAACAAG TTCATCTCAG 
     1381 CCACAGACAC CATCCGGAAG ATGAAGAACG ATTTCCGGAA GATGGAGGAT GAGATGGACC 
     1441 GGCTGGCCAC CAACATGGCA GTGATCACCG ACTTCAGCGC TCGCATCAGC GCCACGCTGC 
     1501 AGGACCGCCA CGAGCGCATC ACCAAGCTGG CAGGGGTCCA CGCGCTGCTG CGGAAGCTGC 
     1561 AGTTCCTCTT TGAGCTGCCC TCGCGCCTCA CCAAGTGCGT GGAACTGGGC GCCTATGGGC 
     1621 AGGCGGTGCG CTACCAGGGC CGCGCGCAGG CCGTGCTGCA GCAGTACCAA CACCTGCCCT 
     1681 CGTTCCGCGC CATCCAGGAC GACTGCCAGG TCATCACGGC CCGCCTGGCC CAGCAGCTGC 
     1741 GGCAGCGCTT TAGGGAGGGC GGCTCAGGCG CCCCGGAGCA GGCAGAGTGC GTGGAGCTGC 
     1801 TGCTGGCCCT GGGCGAGCCT GCGGAGGAGC TGTGCGAGGA GTTCCTGGCG CACGCCCGCG 
     1861 GCCGGCTGGA GAAGGAGCTG AGAAACCTGG AGGCCGAGCT GGGGCCCTCA CCTCCGGCTC 
     1921 CCGACGTGTT AGAGTTCACC GACCATGGAG GCAGTGGCTT CGTGGGCGGC CTCTGCCAGG 
     1981 TGGCGGCGGC CTACCAGGAG CTGTTTGCGG CCCAGGGCCC AGCAGGTGCC GAGAAGCTGG 
     2041 CGGCCTTCGC CCGGCAGCTG GGCAGCCGCT ATTTTGCGCT GGTGGAGCGG CGGCTGGCGC 
     2101 AGGAGCAGGG TGGTGGTGAC AACTCACTGC TGGTGCGGGC GCTGGACCGC TTCCACCGGC 
     2161 GCTTGCGGGC TCCCGGGGCC CTGCTGGCCG CTGCCGGGCT CGCAGACGCT GCCACGGAGA 
     2221 TCGTGGAACG AGTGGCCCGC GAGCGCCTGG GCCACCACCT GCAGGGTCTC CGGGCGGCCT 
     2281 TCCTGGGCTG CCTGACAGAC GTCCGCCAGG CGCTGGCAGC ACCTCGCGTG GCTGGGAAGG 
     2341 AGGGCCCTGG CCTGGCCGAG TTGCTGGCCA ATGTGGCCAG CTCCATCCTG AGCCACATTA 
     2401 AGGCCTCTCT GGCAGCAGTG CACCTTTTCA CCGCCAAAGA GGTGTCCTTC TCCAACAAGC 
     2461 CCTACTTCCG GGGTGAGTTC TGCAGTCAGG GTGTCCGTGA GGGCCTCATC GTGGGCTTCG 
     2521 TCCACTCTAT GTGCCAGACG GCTCAGAGCT TCTGCGACAG CCCTGGGGAG AAGGGGGGTG 
     2581 CCACACCACC TGCCCTGCTC CTGCTGCTCT CCCGCCTCTG CCTGGACTAC GAGACGGCCA 
     2641 CCATCTCCTA CATCCTCACT CTCACTGATG AACAGTTTCT GGTGCAGGAT CAGTTCCCAG 
     2701 TGACGCCCGT GAGCACGCTG TGTGCAGAGG CCAGGGAAAC GGCGCGGCGG CTGCTGACCC 
     2761 ACTACGTGAA GGTGCAGGGC CTGGTCATAT CACAGATGCT GCGCAAGAGC GTGGAGACTC 
     2821 GCGACTGGCT CAGCACTCTG GAGCCCCGGA ATGTGCGGGC CGTCATGAAG CGGGTGGTGG 
     2881 AGGATACCAC CGCCATCGAC GTGCAGGTGG GGCTCCTGTA CGAAGAGGGT GTTCGCAAGG 
     2941 CCCAGAGCAG CGACTCCAGC AAGAGGACTT TCTCCGTGTA CAGCAGCTCT CGGCAGCAGG 
     3001 GCCGCTACGC CCCCAGCTAT ACCCCCAGTG CCCCGATGGA CACCAACCTC TTGAGCAATA 
     3061 TCCAGAAGCT ATTCTCTGAA CGTATTGATG TGTTCAGCCC TGTGGAGTTC AACAAGGTGT 
     3121 CGGTGCTGAC CGGCATCATC AAGATCAGCC TGAAGACGCT GCTGGAGTGT GTGCGGCTGC 
     3181 GCACCTTTGG GCGCTTCGGG CTGCAGCAGG TGCAAGTGGA CTGCCACTTT CTGCAGCTCT 
     3241 ACCTGTGGCG TTTTGTGGCC GACGAAGAAC TCGTGCACTT GCTGCTGGAC GAAGTGGTGG 
     3301 CCTCTGCTGC CCTGCGCTGC CCAGACCCTG TGCCCATGGA GCCCAGTGTG GTTGAGGTCA 
     3361 TCTGCGAGCG CGGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3421 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3481 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3541 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3601 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3661 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3721 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3781 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3841 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3901 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3961 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4021 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4081 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4141 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4201 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4261 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4321 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4381 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4441 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4501 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4561 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4621 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4681 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4741 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4801 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4861 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4921 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4981 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5041 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5101 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5161 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5221 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5281 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5341 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5401 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5461 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5521 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5581 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5641 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5701 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5761 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5821 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5881 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5941 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6001 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6061 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6121 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6181 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6241 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6301 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6361 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6421 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6481 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6541 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6601 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6661 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6721 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6781 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6841 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6901 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6961 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7021 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7081 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7141 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7201 ATTTAACAAA AATTTAACGC GAATTTT
//