LOCUS       RC202651                7113 bp    DNA     circular  SYN 
DEFINITION  CNOT10 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 10 (CNOT10)
ACCESSION   NM_015442
VERSION     NM_015442.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3270..3361 
                     /label="Myc-DDK"  
     misc_feature    3391..3976 
                     /label="PolyA signal"  
     misc_feature    3448..3467 
                     /label="Restriction Enzyme"  
     misc_feature    4252..4924 
                     /label="ColE1"  
     misc_feature    5354..6148 
                     /label="Kan/Neo"  
     misc_feature    6183..6485 
                     /label="SV40 ori"  
     misc_feature    6571..7048 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3252..3258 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3252 
                     /label="CNOT10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCAGAC AAGCCTGCAG ATCAGGGAGC AGAGAAACAT GAAGGCACAG 
     1081 GTCAGTCCTC TGGGATCACT GATCAAGAGA AGGAGTTATC CACCAATGCT TTCCAAGCTT 
     1141 TCACATCTGG AAATTATGAT GCCTGTCTAC AACACCTTGC CTGTCTACAA GATATAAACA 
     1201 AAGATGATTA TAAAATAATT TTGAATACAG CAGTAGCTGA GTTTTTTAAA AGTAACCAAA 
     1261 CAACAACAGA TAATTTGAGA CAAACACTTA ACCAGCTGAA GAATCAGGTC CACTCAGCTG 
     1321 TTGAAGAAAT GGATGGATTA GATGATGTTG AAAACAGCAT GTTGTACTAT AATCAAGCAG 
     1381 TCATTCTTTA TCATCTGCGG CAGTATACAG AAGCCATATC AGTTGGTGAA AAACTTTATC 
     1441 AGTTCATAGA GCCTTTTGAA GAAAAATTTG CCCAAGCAGT GTGTTTTTTG CTTGTAGACC 
     1501 TGTATATATT AACCTACCAA GCTGAGAAAG CTTTGCATCT TCTTGCTGTC CTAGAAAAAA 
     1561 TGATTTCACA GGGTAACAAT AACAAAAATG GAAAGAATGA GACTGGTAAT AACAACAACA 
     1621 AAGATGGATC TAATCATAAA GCTGAAAGTG GAGCTCTAAT AGAAGCTGCA AAATCAAAGA 
     1681 TACATCAGTA CAAAGTACGA GCTTATATCC AAATGAAGTC TCTGAAAGCA TGTAAAAGGG 
     1741 AAATCAAGTC AGTCATGAAT ACAGCTGGAA ATTCCGCACC CTCTCTCTTT CTTAAAAGCA 
     1801 ATTTTGAGTA CTTAAGAGGT AATTATCGAA AAGCCGTGAA GCTATTAAAT AGTTCAAACA 
     1861 TTGCTGAGCA TCCAGGATTC ATGAAAACAG GTGAATGCTT GAGATGCATG TTCTGGAATA 
     1921 ACCTTGGTTG CATCCATTTT GCCATGAGCA AGCACAATTT GGGAATATTC TACTTTAAAA 
     1981 AGGCTCTGCA AGAGAATGAC AATGTCTGTG CACAGCTCAG TGCAGGTAGC ACTGATCCAG 
     2041 GTAAAAAATT TTCAGGAAGA CCCATGTGTA CGTTACTAAC CAATAAGAGA TATGAGTTGC 
     2101 TGTATAACTG TGGAATTCAG CTTCTTCACA TTGGAAGGCC TCTTGCTGCC TTCGAATGTC 
     2161 TGATTGAAGC TGTTCAGGTT TATCATGCAA ATCCTCGCCT CTGGCTACGG CTGGCTGAAT 
     2221 GCTGCATTGC TGCCAATAAG GGGACTTCTG AACAAGAAAC TAAAGGCCTT CCCAGCAAAA 
     2281 AAGGAATTGT ACAGTCTATT GTTGGTCAAG GCTATCATCG TAAAATAGTT TTGGCATCAC 
     2341 AGTCTATACA GAATACTGTT TATAATGATG GTCAGTCTTC GGCCATTCCT GTAGCCAGTA 
     2401 TGGAGTTTGC AGCCATATGT CTCAGAAATG CCTTGTTGCT GCTACCTGAA GAACAGCAAG 
     2461 ATCCAAAGCA GGAAAATGGG GCTAAAAATA GTAATCAATT AGGTGGGAAC ACAGAGAGCA 
     2521 GCGAAAGCAG TGAAACTTGC AGCAGTAAAA GCCATGATGG AGATAAATTC ATTCCAGCTC 
     2581 CACCTTCTTC TCCATTGAGA AAACAGGAAT TAGAAAACTT AAAGTGCTCC ATACTTGCTT 
     2641 GCAGTGCCTA CGTGGCTCTG GCTTTGGGTG ATAACCTCAT GGCTTTGAAT CATGCAGATA 
     2701 AACTTCTTCA GCAGCCCAAG CTGTCAGGAT CTCTTAAGTT TTTGGGACAT TTATATGCTG 
     2761 CAGAAGCCCT CATCTCTCTC GACAGAATAT CTGATGCCAT TACTCACTTG AACCCGGAGA 
     2821 ATGTCACTGA TGTCTCCTTA GGGATCTCTT CAAATGAGCA GGACCAAGGA TCAGACAAAG 
     2881 GTGAAAATGA AGCAATGGAA TCCTCTGGTA AGCGGGCCCC TCAGTGCTAC CCCAGTTCCG 
     2941 TCAACTCTGC CAGGACTGTG ATGCTGTTCA ACCTTGGCAG CGCTTACTGC CTGAGGAGCG 
     3001 AATATGACAA AGCCCGAAAG TGTCTCCACC AGGCGGCTTC AATGATCCAT CCTAAAGAGG 
     3061 TGCCCCCTGA GGCCATCTTG CTGGCAGTCT ACCTTGAACT GCAGAATGGT AATACTCAGC 
     3121 TGGCCTTACA GATCATCAAA AGGAATCAGC TGCTCCCTGC AGTGAAAACA CACTCTGAAG 
     3181 TGAGAAAGAA GCCAGTGTTT CAGCCTGTCC ACCCGATCCA GCCCATCCAA ATGCCGGCTT 
     3241 TCACCACTGT GCAGAGAAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3301 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3361 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3421 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3481 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3541 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3601 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3661 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3721 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3781 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3841 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3901 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3961 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4021 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4081 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4141 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4201 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4261 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4321 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4381 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4441 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4501 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4561 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4621 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4681 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4741 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4801 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4861 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4921 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4981 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5041 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5101 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5161 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5221 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5281 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5341 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5401 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5461 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5521 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5581 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5641 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5701 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5761 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5821 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5881 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5941 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6001 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6061 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6121 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6181 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6241 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6301 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6361 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6421 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6481 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6541 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6601 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6661 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6721 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6781 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6841 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6901 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6961 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7021 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7081 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//