LOCUS RC202532 6744 bp DNA circular SYN DEFINITION MSLN (Myc-DDK-tagged)-Human mesothelin (MSLN), transcript variant 1 ACCESSION NM_005823 VERSION NM_005823.4 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2901..2992 /label="Myc-DDK" misc_feature 3022..3607 /label="PolyA signal" misc_feature 3079..3098 /label="Restriction Enzyme" misc_feature 3883..4555 /label="ColE1" misc_feature 4985..5779 /label="Kan/Neo" misc_feature 5814..6116 /label="SV40 ori" misc_feature 6202..6679 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2883..2889 /label="Restriction Enzyme" misc_feature 1020..2883 /label="MSLN" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCCTTGCCA ACGGCTCGAC CCCTGTTGGG GTCCTGTGGG ACCCCCGCCC 1081 TCGGCAGCCT CCTGTTCCTG CTCTTCAGCC TCGGATGGGT GCAGCCCTCG AGGACCCTGG 1141 CTGGAGAGAC AGGGCAGGCT GCACCCCTGG ACGGAGTCCT GGCCAACCCA CCTAACATTT 1201 CCAGCCTCTC CCCTCGCCAA CTCCTTGGCT TCCCGTGTGC GGAGGTGTCC GGCCTGAGCA 1261 CGGAGCGTGT CCGGGAGCTG GCTGTGGCCT TGGCACAGAA GAATGTCAAG CTCTCAACAG 1321 AGCAGCTGCG CTGTCTGGCT CACCGGCTCT CTGAGCCCCC CGAGGACCTG GACGCCCTCC 1381 CATTGGACCT GCTGCTATTC CTCAACCCAG ATGCGTTCTC GGGGCCCCAG GCCTGCACCC 1441 GTTTCTTCTC CCGCATCACG AAGGCCAATG TGGACCTGCT CCCGAGGGGG GCTCCCGAGC 1501 GACAGCGGCT GCTGCCTGCG GCTCTGGCCT GCTGGGGTGT GCGGGGGTCT CTGCTGAGCG 1561 AGGCTGATGT GCGGGCTCTG GGAGGCCTGG CTTGCGACCT GCCTGGGCGC TTTGTGGCCG 1621 AGTCGGCCGA AGTGCTGCTA CCCCGGCTGG TGAGCTGCCC GGGACCCCTG GACCAGGACC 1681 AGCAGGAGGC AGCCAGGGCG GCTCTGCAGG GCGGGGGACC CCCCTACGGC CCCCCGTCGA 1741 CATGGTCTGT CTCCACGATG GACGCTCTGC GGGGCCTGCT GCCCGTGCTG GGCCAGCCCA 1801 TCATCCGCAG CATCCCGCAG GGCATCGTGG CCGCGTGGCG GCAACGCTCC TCTCGGGACC 1861 CATCCTGGCG GCAGCCTGAA CGGACCATCC TCCGGCCGCG GTTCCGGCGG GAAGTGGAGA 1921 AGACAGCCTG TCCTTCAGGC AAGAAGGCCC GCGAGATAGA CGAGAGCCTC ATCTTCTACA 1981 AGAAGTGGGA GCTGGAAGCC TGCGTGGATG CGGCCCTGCT GGCCACCCAG ATGGACCGCG 2041 TGAACGCCAT CCCCTTCACC TACGAGCAGC TGGACGTCCT AAAGCATAAA CTGGATGAGC 2101 TCTACCCACA AGGTTACCCC GAGTCTGTGA TCCAGCACCT GGGCTACCTC TTCCTCAAGA 2161 TGAGCCCTGA GGACATTCGC AAGTGGAATG TGACGTCCCT GGAGACCCTG AAGGCTTTGC 2221 TTGAAGTCAA CAAAGGGCAC GAAATGAGTC CTCAGGTGGC CACCCTGATC GACCGCTTTG 2281 TGAAGGGAAG GGGCCAGCTA GACAAAGACA CCCTAGACAC CCTGACCGCC TTCTACCCTG 2341 GGTACCTGTG CTCCCTCAGC CCCGAGGAGC TGAGCTCCGT GCCCCCCAGC AGCATCTGGG 2401 CGGTCAGGCC CCAGGACCTG GACACGTGTG ACCCAAGGCA GCTGGACGTC CTCTATCCCA 2461 AGGCCCGCCT TGCTTTCCAG AACATGAACG GGTCCGAATA CTTCGTGAAG ATCCAGTCCT 2521 TCCTGGGTGG GGCCCCCACG GAGGATTTGA AGGCGCTCAG TCAGCAGAAT GTGAGCATGG 2581 ACTTGGCCAC GTTCATGAAG CTGCGGACGG ATGCGGTGCT GCCGTTGACT GTGGCTGAGG 2641 TGCAGAAACT TCTGGGACCC CACGTGGAGG GCCTGAAGGC GGAGGAGCGG CACCGCCCGG 2701 TGCGGGACTG GATCCTACGG CAGCGGCAGG ACGACCTGGA CACGCTGGGG CTGGGGCTAC 2761 AGGGCGGCAT CCCCAACGGC TACCTGGTCC TAGACCTCAG CGTGCAAGAG GCCCTCTCGG 2821 GGACGCCCTG CCTCCTAGGA CCTGGACCTG TTCTCACCGT CCTGGCACTG CTCCTAGCCT 2881 CCACCCTGGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 2941 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 3001 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 3061 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 3121 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 3181 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 3241 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 3301 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 3361 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 3421 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 3481 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 3541 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 3601 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 3661 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 3721 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 3781 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 3841 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 3901 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3961 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 4021 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 4081 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 4141 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 4201 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 4261 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 4321 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 4381 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 4441 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 4501 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 4561 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 4621 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 4681 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 4741 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 4801 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 4861 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 4921 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 4981 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 5041 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 5101 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 5161 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 5221 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 5281 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 5341 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 5401 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 5461 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 5521 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 5581 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 5641 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 5701 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 5761 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 5821 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 5881 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 5941 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 6001 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 6061 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 6121 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 6181 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 6241 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 6301 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 6361 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 6421 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 6481 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 6541 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 6601 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 6661 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 6721 TAACAAAAAT TTAACGCGAA TTTT //