LOCUS       RC202136                7785 bp    DNA     circular  SYN 
DEFINITION  AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS)
ACCESSION   NM_001605
VERSION     NM_001605.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3942..4033 
                     /label="Myc-DDK"  
     misc_feature    4063..4648 
                     /label="PolyA signal"  
     misc_feature    4120..4139 
                     /label="Restriction Enzyme"  
     misc_feature    4924..5596 
                     /label="ColE1"  
     misc_feature    6026..6820 
                     /label="Kan/Neo"  
     misc_feature    6855..7157 
                     /label="SV40 ori"  
     misc_feature    7243..7720 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3924..3930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3924 
                     /label="AARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTACT CTAACAGCAA GTGAAATCCG GCAGCGATTT ATAGATTTCT 
     1081 TCAAGAGGAA CGAGCATACG TATGTTCACT CGTCTGCCAC CATCCCATTG GATGACCCCA 
     1141 CTTTGCTCTT TGCCAATGCA GGCATGAACC AGTTTAAACC CATTTTCCTG AACACAATTG 
     1201 ACCCATCTCA CCCCATGGCA AAGCTGAGCA GAGCTGCCAA TACCCAGAAG TGCATCCGGG 
     1261 CTGGGGGCAA ACATAATGAC CTGGACGATG TGGGCAAGGA TGTCTATCAT CACACCTTCT 
     1321 TCGAGATGCT GGGCTCTTGG TCTTTTGGAG ATTACTTTAA GGAATTGGCA TGTAAGATGG 
     1381 CTCTGGAACT CCTCACCCAA GAGTTTGGCA TTCCCATTGA AAGACTTTAT GTTACTTACT 
     1441 TTGGCGGGGA TGAAGCAGCT GGCTTAGAAG CAGATCTGGA ATGCAAACAG ATCTGGCAAA 
     1501 ATTTGGGGCT GGATGACACC AAAATCCTCC CAGGCAACAT GAAGGATAAC TTCTGGGAGA 
     1561 TGGGTGACAC GGGCCCCTGT GGTCCTTGCA GTGAGATCCA CTACGACCGG ATTGGTGGTC 
     1621 GGGACGCCGC ACATCTTGTC AACCAGGACG ACCCTAATGT GCTGGAGATC TGGAACCTTG 
     1681 TGTTCATCCA GTATAACAGG GAAGCTGATG GCATTCTGAA ACCTCTTCCC AAGAAAAGCA 
     1741 TTGACACAGG GATGGGCCTG GAACGACTGG TATCTGTGCT GCAGAATAAG ATGTCCAACT 
     1801 ATGACACTGA CCTTTTTGTC CCTTACTTTG AAGCCATTCA GAAGGGCACA GGTGCCCGAC 
     1861 CATACACTGG GAAAGTTGGT GCTGAGGATG CCGATGGGAT TGACATGGCC TACCGGGTGC 
     1921 TGGCTGACCA TGCTCGGACC ATCACTGTGG CACTGGCTGA TGGTGGCCGG CCTGACAACA 
     1981 CAGGGCGTGG ATATGTGTTG AGACGGATTC TCCGCCGAGC TGTCCGATAC GCCCATGAAA 
     2041 AGCTCAATGC CAGCAGGGGC TTCTTTGCTA CGTTAGTGGA TGTTGTCGTC CAGTCCCTGG 
     2101 GAGATGCATT TCCTGAGCTG AAGAAGGACC CAGACATGGT GAAGGACATC ATTAATGAAG 
     2161 AAGAGGTGCA GTTTCTCAAG ACTCTCAGCA GAGGGCGTCG CATCCTGGAC AGGAAAATTC 
     2221 AGAGCCTGGG AGACAGCAAG ACCATTCCCG GAGACACTGC TTGGCTCCTC TATGACACCT 
     2281 ATGGGTTTCC AGTGGATCTG ACTGGACTGA TTGCTGAAGA GAAGGGCCTG GTGGTAGACA 
     2341 TGGATGGCTT TGAAGAGGAG AGGAAACTGG CCCAGCTGAA ATCACAGGGC AAGGGAGCTG 
     2401 GTGGGGAAGA CCTCATTATG CTGGACATTT ACGCTATCGA AGAGCTCCGG GCACGGGGTC 
     2461 TGGAGGTCAC AGATGATTCC CCAAAGTACA ATTACCATTT GGACTCCAGT GGTAGCTATG 
     2521 TATTTGAGAA CACAGTGGCT ACGGTGATGG CTCTGCGCAG GGAGAAGATG TTCGTGGAAG 
     2581 AGGTGTCCAC AGGCCAGGAG TGTGGAGTGG TGCTGGACAA GACCTGTTTC TATGCTGAGC 
     2641 AAGGAGGCCA GATCTATGAC GAAGGCTACC TGGTGAAGGT GGATGACAGC AGTGAAGATA 
     2701 AAACAGAGTT TACAGTGAAG AATGCTCAGG TCCGAGGAGG GTATGTGCTA CACATTGGAA 
     2761 CCATCTACGG TGACCTGAAA GTGGGGGATC AGGTCTGGCT GTTTATTGAT GAGCCCCGAC 
     2821 GAAGACCCAT CATGAGCAAC CACACAGCTA CGCACATTCT GAACTTCGCC CTGCGCTCAG 
     2881 TGCTTGGGGA AGCTGACCAG AAAGGCTCAT TGGTTGCTCC TGACCGCCTC AGATTTGACT 
     2941 TTACTGCCAA GGGAGCCATG TCCACCCAAC AGATCAAGAA GGCTGAAGAG ATTGCTAATG 
     3001 AGATGATTGA GGCAGCCAAG GCCGTCTATA CCCAGGATTG CCCCCTGGCA GCAGCGAAAG 
     3061 CCATCCAGGG CCTACGGGCT GTGTTTGATG AGACCTATCC TGACCCTGTG CGAGTCGTCT 
     3121 CCATTGGGGT CCCGGTGTCC GAGTTGCTGG ATGACCCCTC TGGGCCTGCT GGCTCCCTGA 
     3181 CTTCTGTTGA GTTCTGTGGG GGAACGCACC TGCGGAACTC GAGTCATGCA GGAGCTTTTG 
     3241 TGATCGTGAC GGAAGAAGCC ATTGCCAAGG GTATCCGGAG GATTGTGGCT GTCACAGGTG 
     3301 CCGAGGCCCA GAAGGCCCTC AGGAAAGCAG AGAGCTTGAA GAAATGTCTC TCTGTCATGG 
     3361 AAGCCAAAGT GAAGGCTCAG ACTGCTCCAA ACAAGGATGT GCAGAGGGAG ATCGCTGACC 
     3421 TTGGAGAGGC CCTGGCCACT GCAGTCATCC CCCAGTGGCA GAAGGATGAA TTGCGGGAGA 
     3481 CTCTCAAATC CCTAAAGAAG GTCATGGATG ACTTGGACCG AGCCAGCAAA GCCGATGTCC 
     3541 AGAAACGAGT GTTAGAGAAG ACGAAGCAGT TCATCGACAG CAACCCCAAC CAGCCTCTTG 
     3601 TCATCCTGGA GATGGAGAGC GGCGCCTCAG CCAAGGCCCT GAATGAAGCC TTGAAGCTCT 
     3661 TCAAGATGCA CTCCCCTCAG ACTTCTGCCA TGCTCTTCAC GGTGGACAAT GAGGCTGGCA 
     3721 AGATCACGTG CCTGTGTCAA GTCCCCCAGA ATGCAGCCAA TCGGGGCTTA AAAGCCAGCG 
     3781 AGTGGGTGCA GCAGGTGTCA GGCTTGATGG ACGGTAAAGG TGGTGGCAAG GATGTGTCTG 
     3841 CACAGGCCAC AGGCAAGAAC GTTGGCTGCC TGCAGGAGGC GCTGCAGCTG GCCACTTCCT 
     3901 TCGCCCAGCT GCGCCTCGGG GATGTAAAGA ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3961 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4021 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4081 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4141 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4201 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4261 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4321 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4381 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4441 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4501 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4561 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4621 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4681 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4741 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4801 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4861 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4921 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4981 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5041 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5101 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5161 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5221 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5281 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5341 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5401 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5461 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5521 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5581 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5641 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5701 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5761 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5821 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5881 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5941 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6001 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6061 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6121 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6181 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6241 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6301 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6361 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6421 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6481 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6541 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6601 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6661 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6721 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6781 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6841 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6901 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6961 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7021 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7141 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7201 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7261 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7321 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7381 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7441 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7561 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7621 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7681 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7741 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//