LOCUS       RC202073                6909 bp    DNA     circular  SYN 
DEFINITION  PROS1 (Myc-DDK-tagged)-Human protein S (alpha) (PROS1)
ACCESSION   NM_000313
VERSION     NM_000313.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3066..3157 
                     /label="Myc-DDK"  
     misc_feature    3187..3772 
                     /label="PolyA signal"  
     misc_feature    3244..3263 
                     /label="Restriction Enzyme"  
     misc_feature    4048..4720 
                     /label="ColE1"  
     misc_feature    5150..5944 
                     /label="Kan/Neo"  
     misc_feature    5979..6281 
                     /label="SV40 ori"  
     misc_feature    6367..6844 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3048..3054 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3048 
                     /label="PROS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGTCCTG GGTGGGCGCT GCGGGGCGCT GCTGGCGTGT CTCCTCCTAG 
     1081 TGCTTCCCGT CTCAGAGGCA AACTTTTTGT CAAAGCAACA GGCTTCACAA GTCCTGGTTA 
     1141 GGAAGCGTCG TGCAAATTCT TTACTTGAAG AAACCAAACA GGGTAATCTT GAAAGAGAAT 
     1201 GCATCGAAGA ACTGTGCAAT AAAGAAGAAG CCAGGGAGGT CTTTGAAAAT GACCCGGAAA 
     1261 CGGATTATTT TTATCCAAAA TACTTAGTTT GTCTTCGCTC TTTTCAAACT GGGTTATTCA 
     1321 CTGCTGCACG TCAGTCAACT AATGCTTATC CTGACCTAAG AAGCTGTGTC AATGCCATTC 
     1381 CAGACCAGTG TAGTCCTCTG CCATGCAATG AAGATGGATA TATGAGCTGC AAAGATGGAA 
     1441 AAGCTTCTTT TACTTGCACT TGTAAACCAG GTTGGCAAGG AGAAAAGTGT GAATTTGACA 
     1501 TAAATGAATG CAAAGATCCC TCAAATATAA ATGGAGGTTG CAGTCAAATT TGTGATAATA 
     1561 CACCTGGAAG TTACCACTGT TCCTGTAAAA ATGGTTTTGT TATGCTTTCA AATAAGAAAG 
     1621 ATTGTAAAGA TGTGGATGAA TGCTCTTTGA AGCCAAGCAT TTGTGGCACA GCTGTGTGCA 
     1681 AGAACATCCC AGGAGATTTT GAATGTGAAT GCCCCGAAGG CTACAGATAT AATCTCAAAT 
     1741 CAAAGTCTTG TGAAGATATA GATGAATGCT CTGAGAACAT GTGTGCTCAG CTTTGTGTCA 
     1801 ATTACCCTGG AGGTTACACT TGCTATTGTG ATGGGAAGAA AGGATTCAAA CTTGCCCAAG 
     1861 ATCAGAAGAG TTGTGAGGTT GTTTCAGTGT GCCTTCCCTT GAACCTTGAC ACAAAGTATG 
     1921 AATTACTTTA CTTGGCGGAG CAGTTTGCAG GGGTTGTTTT ATATTTAAAA TTTCGTTTGC 
     1981 CAGAAATCAG CAGATTTTCA GCAGAATTTG ATTTCCGGAC ATATGATTCA GAAGGCGTGA 
     2041 TACTGTACGC AGAATCTATC GATCACTCAG CGTGGCTCCT GATTGCACTT CGTGGTGGAA 
     2101 AGATTGAAGT TCAGCTTAAG AATGAACATA CATCCAAAAT CACAACTGGA GGTGATGTTA 
     2161 TTAATAATGG TCTATGGAAT ATGGTGTCTG TGGAAGAATT AGAACATAGT ATTAGCATTA 
     2221 AAATAGCTAA AGAAGCTGTG ATGGATATAA ATAAACCTGG ACCCCTTTTT AAGCCGGAAA 
     2281 ATGGATTGCT GGAAACCAAA GTATACTTTG CAGGATTCCC TCGGAAAGTG GAAAGTGAAC 
     2341 TCATTAAACC GATTAACCCT CGTCTAGATG GATGTATACG AAGCTGGAAT TTGATGAAGC 
     2401 AAGGAGCTTC TGGAATAAAG GAAATTATTC AAGAAAAACA AAATAAGCAT TGCCTGGTTA 
     2461 CTGTGGAGAA GGGCTCCTAC TATCCTGGTT CTGGAATTGC TCAATTTCAC ATAGATTATA 
     2521 ATAATGTATC CAGTGCTGAG GGTTGGCATG TAAATGTGAC CTTGAATATT CGTCCATCCA 
     2581 CGGGCACTGG TGTTATGCTT GCCTTGGTTT CTGGTAACAA CACAGTGCCC TTTGCTGTGT 
     2641 CCTTGGTGGA CTCCACCTCT GAAAAATCAC AGGATATTCT GTTATCTGTT GAAAATACTG 
     2701 TAATATATCG GATACAGGCC CTAAGTCTAT GTTCCGATCA ACAATCTCAT CTGGAATTTA 
     2761 GAGTCAACAG AAACAATCTG GAGTTGTCGA CACCACTTAA AATAGAAACC ATCTCCCATG 
     2821 AAGACCTTCA AAGACAACTT GCCGTCTTGG ACAAAGCAAT GAAAGCAAAA GTGGCCACAT 
     2881 ACCTGGGTGG CCTTCCAGAT GTTCCATTCA GTGCCACACC AGTGAATGCC TTTTATAATG 
     2941 GCTGCATGGA AGTGAATATT AATGGTGTAC AGTTGGATCT GGATGAAGCC ATTTCTAAAC 
     3001 ATAATGATAT TAGAGCTCAC TCATGTCCAT CAGTTTGGAA AAAGACAAAG AATTCTACGC 
     3061 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3121 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3181 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3241 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3301 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3361 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3421 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3481 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3541 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3601 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3661 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3721 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3781 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3841 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3901 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3961 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4021 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4081 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4141 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4201 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4261 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4321 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4381 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4441 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4501 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4561 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4621 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4681 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4741 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4801 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4861 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4921 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4981 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5041 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5101 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5161 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5221 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5281 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5341 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5401 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5461 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5521 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5581 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5641 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5701 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5761 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5821 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5881 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5941 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6001 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6061 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6121 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6181 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6241 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6301 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6361 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6421 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6481 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6541 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6601 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6661 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6721 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6781 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6841 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6901 GCGAATTTT
//