LOCUS       RC202072                7659 bp    DNA     circular  SYN 
DEFINITION  PTPN4 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte) (PTPN4)
ACCESSION   NM_002830
VERSION     NM_002830.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3816..3907 
                     /label="Myc-DDK"  
     misc_feature    3937..4522 
                     /label="PolyA signal"  
     misc_feature    3994..4013 
                     /label="Restriction Enzyme"  
     misc_feature    4798..5470 
                     /label="ColE1"  
     misc_feature    5900..6694 
                     /label="Kan/Neo"  
     misc_feature    6729..7031 
                     /label="SV40 ori"  
     misc_feature    7117..7594 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3798..3804 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3798 
                     /label="PTPN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCACGT TTCCGATTGC CTGCTGGCAG AACCTACAAT GTACGAGCAT 
     1081 CAGAGTTGGC CCGAGACAGA CAGCATACTG AAGTGGTTTG CAACATCCTT CTTCTGGATA 
     1141 ACACTGTACA AGCTTTCAAA GTCAATAAAC ATGATCAGGG GCAAGTCTTG TTGGATGTCG 
     1201 TCTTCAAGCA TCTAGATTTG ACTGAGCAGG ACTATTTTGG TTTACAGTTG GCTGATGATT 
     1261 CCACAGATAA CCCAAGGTGG CTGGATCCAA ACAAACCAAT AAGGAAGCAG CTAAAGAGAG 
     1321 GATCTCCTTA CAGTTTGAAC TTTAGAGTCA AATTTTTTGT AAGTGACCCC AACAAGTTAC 
     1381 AAGAAGAATA TACAAGGTAC CAGTATTTTT TGCAAATTAA ACAAGACATT CTTACTGGAA 
     1441 GATTACCCTG TCCTTCTAAT ACTGCTGCCC TTTTAGCTTC ATTTGCTGTT CAGTCTGAAC 
     1501 TTGGAGACTA CGATCAGTCA GAGAACTTGT CAGGCTACCT CTCAGATTAT TCTTTCATTC 
     1561 CTAATCAACC TCAAGATTTT GAAAAAGAAA TTGCAAAATT ACATCAGCAA CACATAGGCT 
     1621 TATCTCCTGC AGAAGCAGAA TTTAATTACC TAAACACAGC ACGTACCTTA GAACTCTATG 
     1681 GAGTTGAATT CCACTATGCA AGGGATCAGA GTAACAATGA AATTATGATT GGAGTGATGT 
     1741 CAGGAGGAAT TCTGATTTAT AAGAACAGGG TACGAATGAA TACCTTTCCA TGGTTGAAGA 
     1801 TTGTAAAAAT TTCTTTTAAG TGCAAACAGT TTTTTATTCA ACTTAGAAAA GAATTGCATG 
     1861 AATCTAGAGA AACATTATTG GGATTTAATA TGGTGAATTA CAGAGCATGT AAAAATTTGT 
     1921 GGAAAGCATG TGTAGAACAT CACACATTCT TCCGTTTGGA CAGACCACTT CCACCTCAAA 
     1981 AGAATTTTTT TGCACATTAT TTTACATTAG GTTCAAAATT CCGGTACTGT GGGAGAACTG 
     2041 AAGTCCAATC AGTTCAGTAT GGCAAAGAAA AGGCAAATAA AGACAGGGTA TTTGCAAGAT 
     2101 CCCCAAGTAA GCCCTTGGCA CGGAAATTAA TGGATTGGGA AGTAGTAAGC AGAAATTCAA 
     2161 TATCTGATGA CAGGTTAGAA ACACAAAGTC TTCCATCACG ATCTCCACCG GGAACTCCTA 
     2221 ATCATCGAAA TTCTACATTC ACGCAGGAAG GAACCCGGTT ACGACCATCT TCAGTTGGTC 
     2281 ATTTGGTAGA CCATATGGTT CATACTTCCC CAAGCGAAGT GTTTGTAAAT CAGAGATCTC 
     2341 CGTCATCAAC ACAAGCTAAT AGCATTGTTC TGGAATCATC ACCATCACAA GAGACCCCTG 
     2401 GAGATGGGAA GCCTCCAGCT TTACCACCCA AACAGTCAAA GAAAAACAGT TGGAACCAAA 
     2461 TTCATTATTC ACATTCGCAA CAAGATCTAG AAAGTCATAT TAATGAAACA TTTGATATTC 
     2521 CATCTTCTCC TGAAAAACCC ACTCCTAATG GTGGTATTCC ACATGATAAT CTTGTCCTAA 
     2581 TCAGAATGAA ACCTGATGAA AATGGGAGGT TTGGATTCAA TGTAAAGGGA GGATATGATC 
     2641 AGAAGATGCC TGTGATTGTG TCTCGAGTAG CACCAGGAAC ACCTGCTGAC CTCTGTGTCC 
     2701 CTAGACTGAA TGAAGGGGAC CAAGTTGTAC TGATCAATGG TCGGGACATT GCAGAACACA 
     2761 CTCATGATCA GGTTGTGCTG TTTATTAAAG CTAGTTGTGA GAGACATTCT GGGGAACTCA 
     2821 TGCTTCTAGT TCGACCTAAT GCTGTATATG ATGTAGTGGA AGAAAAGCTA GAAAATGAGC 
     2881 CAGATTTCCA GTATATTCCT GAGAAAGCCC CACTAGATAG TGTGCATCAG GATGACCATT 
     2941 CCCTGCGGGA GTCAATGATC CAGCTAGCTG AGGGGCTTAT CACTGGAACA GTCCTGACAC 
     3001 AGTTTGATCA ACTGTATCGG AAAAAACCTG GAATGACAAT GTCCTGTGCC AAATTACCTC 
     3061 AGAATATTTC CAAAAATAGA TACAGAGATA TTTCGCCTTA TGATGCCACA CGGGTCATTT 
     3121 TAAAAGGTAA TGAAGACTAC ATCAATGCGA ACTATATAAA TATGGAAATT CCTTCTTCCA 
     3181 GCATTATAAA TCAGTACATT GCTTGTCAAG GGCCATTACC ACACACTTGT ACAGATTTTT 
     3241 GGCAGATGAC TTGGGAACAA GGCTCCTCTA TGGTTGTAAT GTTGACCACA CAAGTTGAAC 
     3301 GTGGCAGAGT TAAATGTCAC CAATATTGGC CAGAACCCAC AGGCAGTTCA TCTTATGGAT 
     3361 GCTACCAAGT TACCTGCCAC TCTGAAGAAG GAAACACTGC CTATATCTTC AGGAAGATGA 
     3421 CCCTATTTAA CCAAGAGAAA AATGAAAGTC GTCCACTCAC TCAGATCCAG TACATAGCCT 
     3481 GGCCTGACCA TGGAGTCCCT GATGATTCGA GTGACTTTCT AGATTTTGTT TGTCATGTAC 
     3541 GAAACAAGAG GGCTGGCAAG GAAGAACCCG TTGTTGTCCA TTGCAGTGCT GGAATCGGAA 
     3601 GAACTGGGGT TCTTATTACT ATGGAAACAG CCATGTGTCT CATTGAATGC AATCAGCCAG 
     3661 TTTATCCACT AGATATTGTA AGAACAATGA GAGATCAGCG AGCCATGATG ATCCAAACAC 
     3721 CTAGTCAATA CAGATTTGTA TGTGAAGCTA TTTTGAAAGT TTATGAAGAA GGCTTTGTTA 
     3781 AACCCTTAAC AACATCAACA AATAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3841 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3901 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3961 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4021 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4081 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4141 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4201 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4261 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4321 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4381 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4441 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4501 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4561 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4621 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4681 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4741 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4801 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4861 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4921 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4981 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5041 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5101 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5161 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5221 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5281 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5341 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5401 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5461 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5521 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5581 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5641 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5701 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5761 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5821 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5881 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5941 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6001 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6061 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6121 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6181 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6241 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6301 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6361 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6421 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6481 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6541 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6601 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6661 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6721 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6781 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6841 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6901 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6961 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7021 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7081 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7141 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7201 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7261 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7321 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7381 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7441 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7501 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7561 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7621 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//