LOCUS       RC201126                7188 bp    DNA     circular  SYN 
DEFINITION  ITGB2 (Myc-DDK-tagged)-Human integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), transcript variant 1
ACCESSION   NM_000211
VERSION     NM_000211.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3345..3436 
                     /label="Myc-DDK"  
     misc_feature    3466..4051 
                     /label="PolyA signal"  
     misc_feature    3523..3542 
                     /label="Restriction Enzyme"  
     misc_feature    4327..4999 
                     /label="ColE1"  
     misc_feature    5429..6223 
                     /label="Kan/Neo"  
     misc_feature    6258..6560 
                     /label="SV40 ori"  
     misc_feature    6646..7123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3327..3333 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3327 
                     /label="ITGB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGGCCTG CGCCCCCCAC TTCTCGCCCT GGTGGGGCTG CTCTCCCTCG 
     1081 GGTGCGTCCT CTCTCAGGAG TGCACGAAGT TCAAGGTCAG CAGCTGCCGG GAATGCATCG 
     1141 AGTCGGGGCC CGGCTGCACC TGGTGCCAGA AGCTGAACTT CACAGGGCCG GGGGATCCTG 
     1201 ACTCCATTCG CTGCGACACC CGGCCACAGC TGCTCATGAG GGGCTGTGCG GCTGACGACA 
     1261 TCATGGACCC CACAAGCCTC GCTGAAACCC AGGAAGACCA CAATGGGGGC CAGAAGCAGC 
     1321 TGTCCCCACA AAAAGTGACG CTTTACCTGC GACCAGGCCA GGCAGCAGCG TTCAACGTGA 
     1381 CCTTCCGGCG GGCCAAGGGC TACCCCATCG ACCTGTACTA TCTGATGGAC CTCTCCTACT 
     1441 CCATGCTTGA TGACCTCAGG AATGTCAAGA AGCTAGGTGG CGACCTGCTC CGGGCCCTCA 
     1501 ACGAGATCAC CGAGTCCGGC CGCATTGGCT TCGGGTCCTT CGTGGACAAG ACCGTGCTGC 
     1561 CGTTCGTGAA CACGCACCCT GATAAGCTGC GAAACCCATG CCCCAACAAG GAGAAAGAGT 
     1621 GCCAGCCCCC GTTTGCCTTC AGGCACGTGC TGAAGCTGAC CAACAACTCC AACCAGTTTC 
     1681 AGACCGAGGT CGGGAAGCAG CTGATTTCCG GAAACCTGGA TGCACCCGAG GGTGGGCTGG 
     1741 ACGCCATGAT GCAGGTCGCC GCCTGCCCGG AGGAAATCGG CTGGCGCAAC GTCACGCGGC 
     1801 TGCTGGTGTT TGCCACTGAT GACGGCTTCC ATTTCGCGGG CGACGGGAAG CTGGGCGCCA 
     1861 TCCTGACCCC CAACGACGGC CGCTGTCACC TGGAGGACAA CTTGTACAAG AGGAGCAACG 
     1921 AATTCGACTA CCCATCGGTG GGCCAGCTGG CGCACAAGCT GGCTGAAAAC AACATCCAGC 
     1981 CCATCTTCGC GGTGACCAGT AGGATGGTGA AGACCTACGA GAAACTCACC GAGATCATCC 
     2041 CCAAGTCAGC CGTGGGGGAG CTGTCTGAGG ACTCCAGCAA TGTGGTCCAT CTCATTAAGA 
     2101 ATGCTTACAA TAAACTCTCC TCCAGGGTAT TCCTGGATCA CAACGCCCTC CCCGACACCC 
     2161 TGAAAGTCAC CTACGACTCC TTCTGCAGCA ATGGAGTGAC GCACAGGAAC CAGCCCAGAG 
     2221 GTGACTGTGA TGGCGTGCAG ATCAATGTCC CGATCACCTT CCAGGTGAAG GTCACGGCCA 
     2281 CAGAGTGCAT CCAGGAGCAG TCGTTTGTCA TCCGGGCGCT GGGCTTCACG GACATAGTGA 
     2341 CCGTGCAGGT CCTTCCCCAG TGTGAGTGCC GGTGCCGGGA CCAGAGCAGA GACCGCAGCC 
     2401 TCTGCCATGG CAAGGGCTTC TTGGAGTGCG GCATCTGCAG GTGTGACACT GGCTACATTG 
     2461 GGAAAAACTG TGAGTGCCAG ACACAGGGCC GGAGCAGCCA GGAGCTGGAA GGAAGCTGCC 
     2521 GGAAGGACAA CAACTCCATC ATCTGCTCAG GGCTGGGGGA CTGTGTCTGC GGGCAGTGCC 
     2581 TGTGCCACAC CAGCGACGTC CCCGGCAAGC TGATATACGG GCAGTACTGC GAGTGTGACA 
     2641 CCATCAACTG TGAGCGCTAC AACGGCCAGG TCTGCGGCGG CCCGGGGAGG GGGCTCTGCT 
     2701 TCTGCGGGAA GTGCCGCTGC CACCCGGGCT TTGAGGGCTC AGCGTGCCAG TGCGAGAGGA 
     2761 CCACTGAGGG CTGCCTGAAC CCGCGGCGTG TTGAGTGTAG TGGTCGTGGC CGGTGCCGCT 
     2821 GCAACGTATG CGAGTGCCAT TCAGGCTACC AGCTGCCTCT GTGCCAGGAG TGCCCCGGCT 
     2881 GCCCCTCACC CTGTGGCAAG TACATCTCCT GCGCCGAGTG CCTGAAGTTC GAAAAGGGCC 
     2941 CCTTTGGGAA GAACTGCAGC GCGGCGTGTC CGGGCCTGCA GCTGTCGAAC AACCCCGTGA 
     3001 AGGGCAGGAC CTGCAAGGAG AGGGACTCAG AGGGCTGCTG GGTGGCCTAC ACGCTGGAGC 
     3061 AGCAGGACGG GATGGACCGC TACCTCATCT ATGTGGATGA GAGCCGAGAG TGTGTGGCAG 
     3121 GCCCCAACAT CGCCGCCATC GTCGGGGGCA CCGTGGCAGG CATCGTGCTG ATCGGCATTC 
     3181 TCCTGCTGGT CATCTGGAAG GCTCTGATCC ACCTGAGCGA CCTCCGGGAG TACAGGCGCT 
     3241 TTGAGAAGGA GAAGCTCAAG TCCCAGTGGA ACAATGATAA TCCCCTTTTC AAGAGCGCCA 
     3301 CCACGACGGT CATGAACCCC AAGTTTGCTG AGAGTACGCG TACGCGGCCG CTCGAGCAGA 
     3361 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3421 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3481 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3541 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3601 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3661 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3721 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3781 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3841 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3901 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3961 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4021 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4081 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4141 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4201 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4861 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4921 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4981 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5041 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5101 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5161 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5221 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5281 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5341 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5401 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5461 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5521 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5581 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5641 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5701 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5761 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5821 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5881 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5941 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6001 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6061 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6121 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6181 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6241 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6301 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6361 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6421 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6601 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6661 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6721 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6781 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6841 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6901 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6961 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7021 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7081 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7141 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//