LOCUS       RC201089                8673 bp    DNA     circular  SYN 
DEFINITION  VARS (Myc-DDK-tagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein
ACCESSION   NM_006295
VERSION     NM_006295.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4830..4921 
                     /label="Myc-DDK"  
     misc_feature    4951..5536 
                     /label="PolyA signal"  
     misc_feature    5008..5027 
                     /label="Restriction Enzyme"  
     misc_feature    5812..6484 
                     /label="ColE1"  
     misc_feature    6914..7708 
                     /label="Kan/Neo"  
     misc_feature    7743..8045 
                     /label="SV40 ori"  
     misc_feature    8131..8608 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4812..4818 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4812 
                     /label="VARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCACCCTC TACGTCTCCC CTCACCCAGA TGCCTTCCCC AGCCTCCGAG 
     1081 CCCTCATAGC CGCTCGCTAT GGGGAGGCTG GGGAGGGTCC CGGATGGGGA GGAGCCCACC 
     1141 CCCGCATCTG TCTCCAGCCA CCCCCGACTA GCAGGACTAG CTTTCCCCCA CCCCGCCTGC 
     1201 CGGCCCTGGA GCAGGGGCCC GGTGGGCTCT GGGTGTGGGG GGCCACGGCT GTGGCCCAGC 
     1261 TGCTGTGGCC AGCAGGCCTG GGGGGCCCAG GGGGCAGCCG GGCGGCTGTC CTTGTCCAAC 
     1321 AGTGGGTCAG TTACGCCGAC ACGGAGTTAA TACCAGCTGC CTGTGGAGCA ACGCTGCCGG 
     1381 CCCTGGGACT CCGAAGCTCG GCCCAGGACC CCCAGGCTGT GCTGGGGGCC CTGGGCAGGG 
     1441 CCCTGAGCCC CTTGGAGGAG TGGCTTCGGC TGCACACCTA CTTGGCCGGG GAGGCCCCCA 
     1501 CTCTGGCTGA CCTGGCGGCT GTCACAGCCT TGCTGCTGCC TTTCCGATAC GTCCTAGACC 
     1561 CACCTGCCCG CCGGATCTGG AATAATGTGA CTCGCTGGTT TGTCACGTGT GTCCGGCAGC 
     1621 CAGAATTCCG AGCCGTGCTA GGAGAAGTGG TTCTATACTC AGGAGCCAGG CCTCTCTCTC 
     1681 ATCAGCCAGG CCCCGAGGCT CCTGCCCTCC CAAAGACAGC TGCTCAGCTC AAGAAAGAGG 
     1741 CAAAGAAACG GGAGAAGCTA GAGAAATTCC AACAGAAGCA GAAGATCCAA CAGCAGCAGC 
     1801 CACCTCCAGG GGAGAAGAAA CCAAAACCAG AGAAGAGGGA GAAACGGGAT CCTGGGGTCA 
     1861 TTACCTATGA CCTCCCAACC CCACCCGGGG AAAAGAAAGA TGTCAGTGGC CCCATGCCCG 
     1921 ACTCCTACAG CCCTCGGTAT GTGGAGGCTG CCTGGTACCC TTGGTGGGAG CAGCAGGGCT 
     1981 TCTTCAAGCC AGAGTATGGG CGTCCTAATG TGTCAGCAGC AAATCCCCGA GGTGTCTTCA 
     2041 TGATGTGCAT CCCACCCCCC AATGTGACAG GCTCCCTGCA CCTGGGCCAT GCACTCACCA 
     2101 ACGCCATCCA GGACTCCCTG ACTCGATGGC ACCGCATGCG TGGGGAGACC ACCCTGTGGA 
     2161 ACCCTGGCTG TGACCATGCA GGTATTGCCA CCCAGGTGGT GGTGGAGAAG AAGCTATGGC 
     2221 GTGAGCAGGG ACTGAGCCGG CACCAGCTGG GCCGCGAGGC CTTTCTACAG GAAGTCTGGA 
     2281 AGTGGAAGGA GGAGAAAGGT GACCGGATTT ACCACCAGTT GAAGAAGCTT GGCAGCTCCT 
     2341 TGGACTGGGA TCGAGCCTGT TTTACCATGG ACCCTAAACT CTCAGCAGCT GTGACAGAGG 
     2401 CCTTTGTCCG GCTTCACGAG GAAGGCATCA TCTATCGCAG TACCCGCCTT GTTAACTGGT 
     2461 CCTGCACCCT CAACTCCGCC ATCTCTGACA TTGAGGTGGA TAAGAAGGAG CTGACAGGTC 
     2521 GCACCCTGCT CTCCGTGCCT GGCTACAAGG AGAAGGTGGA GTTCGGGGTC CTCGTGTCCT 
     2581 TTGCCTATAA GGTCCAAGGC TCAGATAGCG ACGAGGAGGT GGTGGTGGCA ACAACTCGGA 
     2641 TCGAGACAAT GCTGGGAGAT GTGGCTGTAG CTGTGCACCC CAAAGATACC AGATACCAGC 
     2701 ACCTGAAGGG GAAGAACGTG ATCCACCCAT TCCTGTCTCG GAGCCTTCCC ATTGTCTTCG 
     2761 ATGAATTTGT GGACATGGAC TTTGGCACAG GTGCTGTGAA GATCACCCCC GCACATGACC 
     2821 AAAATGACTA TGAAGTTGGG CAGCGGCACG GGCTGGAGGC CATCAGCATC ATGGACTCCC 
     2881 GGGGGGCCCT CATCAATGTG CCTCCGCCTT TCCTGGGCCT GCCCAGGTTT GAGGCCAGGA 
     2941 AAGCGGTGCT GGTGGCGCTG AAGGAGCGGG GACTGTTCCG TGGCATTGAG GACAACCCCA 
     3001 TGGTGGTGCC ACTTTGCAAC CGGTCGAAGG ACGTGGTAGA GCCTCTGCTG CGGCCGCAGT 
     3061 GGTACGTTCG CTGCGGGGAG ATGGCCCAGG CTGCCAGCGC CGCTGTGACT CGGGGTGACC 
     3121 TCCGCATCCT GCCTGAGGCC CATCAGCGCA CATGGCATGC CTGGATGGAC AACATCCGGG 
     3181 AGTGGTGCAT TTCCAGGCAG CTGTGGTGGG GCCATCGCAT CCCAGCCTAC TTTGTCACTG 
     3241 TCAGTGACCC AGCGGTGCCC CCTGGGGAGG ACCCTGATGG GCGGTACTGG GTGAGTGGAC 
     3301 GCAATGAGGC GGAGGCCCGG GAGAAGGCAG CCAAGGAGTT CGGAGTGTCC CCTGACAAGA 
     3361 TCAGTCTCCA GCAAGATGAG GATGTATTGG ATACCTGGTT CTCCTCTGGC CTCTTCCCCT 
     3421 TATCCATTTT GGGCTGGCCC AACCAGTCAG AAGACCTGAG TGTGTTCTAC CCCGGGACAC 
     3481 TGCTGGAGAC CGGTCATGAC ATCCTCTTCT TCTGGGTGGC CCGGATGGTC ATGCTGGGCC 
     3541 TGAAGCTCAC GGGCAGGCTG CCCTTTAGAG AGGTCTACCT CCATGCCATC GTGCGAGATG 
     3601 CTCACGGCCG GAAGATGAGC AAGTCTCTAG GCAATGTCAT CGATCCCCTG GACGTCATCT 
     3661 ATGGAATCTC CCTGCAGGGC CTCCACAACC AGCTGCTGAA CAGCAACCTG GATCCCAGCG 
     3721 AGGTGGAGAA GGCCAAAGAA GGGCAGAAAG CTGACTTCCC AGCGGGGATT CCTGAATGTG 
     3781 GCACCGATGC TCTCCGGTTT GGATTATGTG CCTACATGTC CCAGGGTCGT GACATCAACC 
     3841 TGGATGTGAA CCGGATACTG GGTTACCGCC ACTTCTGCAA CAAGCTCTGG AATGCCACCA 
     3901 AGTTTGCCCT TCGTGGCCTT GGGAAGGGTT TTGTGCCCTC ACCCACCTCC CAGCCCGGAG 
     3961 GCCATGAGAG CCTGGTGGAC CGCTGGATCC GCAGCCGCCT GACAGAGGCT GTGAGGCTCA 
     4021 GCAATCAAGG CTTCCAGGCC TACGACTTCC CGGCCGTCAC CACTGCCCAG TACAGCTTCT 
     4081 GGCTCTATGA GCTCTGTGAT GTCTACTTGG AGTGCCTGAA ACCTGTACTG AATGGGGTGG 
     4141 ACCAGGTGGC AGCTGAGTGT GCCCGCCAGA CCCTGTACAC TTGCCTGGAC GTTGGCCTGC 
     4201 GGCTGCTCTC ACCCTTCATG CCCTTCGTGA CGGAGGAGCT GTTCCAGAGG CTGCCCCGGA 
     4261 GGATGCCGCA AGCTCCCCCT AGCCTCTGTG TTACCCCCTA CCCGGAGCCC TCAGAGTGCT 
     4321 CCTGGAAGGA CCCCGAGGCA GAAGCCGCCC TTGAGCTGGC GCTAAGCATC ACGCGAGCCG 
     4381 TGCGCTCCCT GCGGGCCGAC TACAACCTCA CCCGGATCCG GCCTGACTGT TTCCTGGAAG 
     4441 TGGCGGATGA GGCCACGGGC GCCCTGGCAT CGGCGGTGTC GGGCTACGTG CAGGCCCTGG 
     4501 CCAGCGCAGG TGTGGTGGCT GTTCTGGCCC TGGGGGCTCC CGCCCCCCAG GGTTGCGCTG 
     4561 TGGCTCTGGC TTCTGATCGC TGCTCCATCC ACCTGCAGCT TCAGGGGCTG GTGGACCCTG 
     4621 CACGGGAGCT GGGCAAGCTG CAAGCCAAGC GAGTTGAGGC CCAGCGGCAG GCCCAGCGTC 
     4681 TGCGGGAACG CCGTGCTGCC TCGGGCTATC CTGTCAAGGT GCCGCTCGAA GTCCAGGAGG 
     4741 CAGATGAAGC CAAGCTCCAA CAGACAGAAG CAGAGCTCAG GAAGGTGGAT GAGGCCATCG 
     4801 CCCTATTCCA GAAGATGCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4861 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4921 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4981 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5041 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5101 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5161 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5221 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5281 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5341 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5401 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5461 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5521 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5581 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5641 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5701 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5761 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5821 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5881 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5941 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6001 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6061 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6121 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6181 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6241 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6301 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6361 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6421 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6481 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6541 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6601 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6661 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6721 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6781 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6841 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6901 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6961 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7021 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7081 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7141 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7201 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7261 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7321 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7381 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7441 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7501 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7561 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7621 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7681 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7741 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7801 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7861 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7921 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8101 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8161 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8221 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8281 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8341 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8401 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8461 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8521 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8581 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8641 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//