LOCUS       RC201046                8538 bp    DNA     circular  SYN 
DEFINITION  TONSL (Myc-DDK-tagged)-Human tonsoku-like, DNA repair protein (TONSL)
ACCESSION   NM_013432
VERSION     NM_013432.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="TONSL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGACCCGC CTCTATCTCA ACCTGGGCCT CACCTTTGAG AGCCTGCAGC 
     1081 AGACAGCCCT GTGCAACGAT TACTTCAGGA AGAGCATCTT CCTTGCGGAG CAGAACCACC 
     1141 TTTACGAGGA CCTATTCCGC GCCCGCTACA ACCTGGGCAC CATCCACTGG CGCGCGGGCC 
     1201 AGCACTCCCA GGCTATGCGC TGCTTGGAGG GTGCCCGGGA GTGTGCGCAC ACCATGAGGA 
     1261 AGCGGTTCAT GGAGAGCGAG TGCTGCGTGG TTATTGCACA GGTCCTCCAA GACCTGGGAG 
     1321 ACTTTTTGGC TGCCAAGCGA GCCCTGAAGA AGGCCTACAG GCTGGGCTCC CAGAAGCCTG 
     1381 TGCAGAGGGC AGCCATCTGT CAGAACCTCC AGCATGTGCT GGCAGTGGTC CGGCTGCAGC 
     1441 AACAGCTGGA AGAGGCTGAG GGCAGAGACC CTCAGGGTGC CATGGTCATC TGTGAGCAGC 
     1501 TAGGGGACCT CTTCTCCAAG GCAGGAGACT TTCCCAGGGC AGCTGAGGCT TACCAGAAGC 
     1561 AGCTGCGTTT TGCTGAGCTG CTGGACAGAC CGGGTGCTGA GCGGGCCATC ATCCACGTGT 
     1621 CCCTGGCCAC CACACTGGGA GACATGAAGG ACCACCATGG GGCCGTGCGC CACTATGAGG 
     1681 AGGAACTGAG GCTGCGCAGC GGCAACGTGC TGGAGGAGGC CAAGACCTGG CTGAACATTG 
     1741 CACTGTCCCG CGAGGAGGCC GGCGATGCCT ACGAGCTGCT GGCCCCGTGC TTCCAGAAAG 
     1801 CGCTCAGCTG TGCCCAGCAG GCCCAGCGTC CCCAGCTGCA GAGGCAGGTC TTGCAGCATC 
     1861 TCCATACCGT GCAGCTGAGG CTGCAGCCCC AGGAGGCCCC TGAGACCGAA ACCAGACTGC 
     1921 GGGAGCTCAG TGTAGCTGAA GATGAAGATG AGGAGGAGGA GGCGGAGGAG GCGGCAGCCA 
     1981 CAGCGGAGAG CGAAGCCCTG GAGGCCGGCG AGGTGGAGCT CTCAGAGGGC GAGGACGACA 
     2041 CCGATGGCCT GACCCCGCAG CTGGAGGAGG ACGAGGAGCT TCAGGGCCAC CTGGGCCGGC 
     2101 GGAAGGGGAG CAAGTGGAAC CGGCGAAACG ACATGGGGGA GACCCTGCTG CACCGAGCCT 
     2161 GCATCGAGGG CCAGCTGCGC CGCGTCCAGG ACCTTGTGAG GCAGGGCCAC CCCCTTAACC 
     2221 CTCGGGACTA CTGTGGCTGG ACACCTCTGC ACGAGGCCTG CAACTACGGG CATCTAGAAA 
     2281 TTGTCCGCTT CCTGCTGGAC CACGGGGCCG CAGTGGACGA CCCAGGTGGC CAGGGCTGCG 
     2341 AAGGCATCAC CCCCCTCCAC GATGCCCTCA ACTGTGGCCA CTTCGAGGTG GCTGAGCTGC 
     2401 TGCTTGAACG GGGGGCGTCC GTCACCCTCC GCACTCGAAA GGGCCTCAGC CCGCTGGAGA 
     2461 CGCTGCAGCA GTGGGTGAAG CTGTACCGCA GGGACCTGGA CCTGGAGACG CGGCAGAAGG 
     2521 CCAGGGCCAT GGAGATGCTG CTCCAGGCGG CTGCCTCGGG CCAAGATCCC CACAGCTCCC 
     2581 AGGCCTTCCA CACCCCAAGC AGCCTTCTGT TTGACCCCGA GACCTCTCCT CCTTTGAGCC 
     2641 CCTGCCCAGA ACCCCCCTCT AATAGCACTA GACTCCCAGA GGCCTCTCAG GTCCATGTCA 
     2701 GGGTCTCCCC AGGGCAGGCG GCACCAGCCA TGGCCAGGCC TCGGAGGAGC AGGCATGGGC 
     2761 CAGCCAGCAG CAGCAGCAGC TCAGAAGGCG AGGACAGCGC AGGCCCCGCA CGGCCGTCCC 
     2821 AGAAGAGGCC TCGGTGCTCG GCCACAGCAC AACGGGTGGC AGCCTGGACG CCTGGCCCCG 
     2881 CCAGCAACAG GGAAGCAGCC ACAGCCAGCA CCAGCCGGGC AGCCTACCAG GCAGCCATCC 
     2941 GGGGTGTGGG CAGTGCTCAG AGCCGGCTGG GGCCTGGCCC ACCGCGGGGC CACAGCAAAG 
     3001 CCCTTGCCCC CCAGGCAGCG CTCATCCCGG AGGAGGAGTG CCTGGCTGGG GACTGGCTGG 
     3061 AGCTGGACAT GCCCCTGACC CGCAGCCGCC GGCCCCGCCC CCGGGGCACT GGAGACAACC 
     3121 GCAGGCCCAG TAGTACCTCT GGGTCGGACA GTGAGGAGAG CAGGCCCCGT GCCCGAGCCA 
     3181 AGCAGGTCCG CCTGACCTGC ATGCAGAGTT GCAGTGCGCC AGTTAACGCA GGGCCCAGCA 
     3241 GCCTGGCTTC AGAACCTCCA GGGAGCCCCA GCACCCCCAG GGTCTCAGAG CCCAGTGGGG 
     3301 ACAGCTCTGC GGCAGGCCAG CCCTTGGGTC CGGCCCCGCC CCCTCCCATC CGGGTTCGAG 
     3361 TTCAAGTTCA GGATCATCTC TTCCTCATCC CTGTCCCACA CAGCAGTGAC ACCCACTCTG 
     3421 TGGCCTGGCT GGCCGAGCAG GCGGCCCAGC GCTACTACCA GACCTGCGGG CTGCTGCCCA 
     3481 GGCTCACCCT ACGGAAAGAG GGGGCCCTGC TGGCCCCACA GGACCTCATC CCTGATGTGC 
     3541 TGCAGAGCAA TGACGAGGTG TTGGCTGAGG TGACTTCGTG GGACCTGCCC CCGTTGACTG 
     3601 ACCGCTACCG CAGGGCCTGC CAGAGCCTGG GGCAAGGGGA GCACCAACAG GTGCTGCAGG 
     3661 CCGTGGAGCT CCAGGGCTTG GGCCTCTCGT TCAGCGCCTG CTCCCTGGCC CTGGACCAGG 
     3721 CCCAGCTTAC ACCCCTGCTG CGGGCCCTCA AGCTGCACAC AGCACTCCGG GAGCTGCGCC 
     3781 TGGCAGGGAA CCGGCTGGGG GACAAGTGTG TGGCTGAGCT GGTGGCTGCC CTGGGCACCA 
     3841 TGCCCAGCCT GGCCCTCCTT GACCTCTCCT CCAATCACCT GGGTCCCGAA GGCCTGCGCC 
     3901 AGCTTGCCAT GGGGCTCCCA GGCCAAGCCA CCTTGCAGAG TTTGGAGGAG CTGGACTTAA 
     3961 GCATGAACCC CCTGGGGGAC GGCTGTGGCC AGTCCCTGGC CTCCCTCCTG CACGCCTGCC 
     4021 CCTTACTCAG CACCCTGCGC CTGCAGGCGT GTGGCTTCGG CCCCAGCTTC TTTCTGAGCC 
     4081 ACCAGACAGC ACTGGGTAGT GCTTTCCAAG ATGCTGAGCA CCTGAAGACC CTGTCCCTGT 
     4141 CCTACAACGC CCTGGGAGCC CCTGCCCTGG CCAGGACCCT GCAGAGCCTG CCCGCCGGCA 
     4201 CCCTCCTGCA CTTAGAGCTC AGCTCCGTGG CAGCCGGCAA GGGTGATTCG GACCTCATGG 
     4261 AGCCTGTATT CCGATACCTG GCCAAGGAAG GCTGTGCTCT AGCCCACCTG ACCCTGTCTG 
     4321 CAAACCACCT GGGGGACAAG GCTGTTAGAG ACCTGTGCAG ATGTCTCTCT CTGTGCCCCT 
     4381 CACTCATCTC ACTGGATCTG TCTGCCAACC CTGAGATCAG CTGTGCCAGC TTGGAAGAGC 
     4441 TCCTGTCCAC CCTCCAAAAG CGGCCCCAAG GCCTTAGCTT CCTTGGCCTG TCAGGCTGCG 
     4501 CCGTCCAGGG TCCCCTGGGC CTGGGCCTGT GGGACAAGAT AGCCGCGCAG CTCCGGGAAC 
     4561 TGCAGCTGTG CAGCAGACGC CTCTGCGCTG AGGACAGGGA CGCCCTGCGC CAGCTGCAGC 
     4621 CCAGTCGGCC GGGCCCCGGC GAGTGCACGC TGGACCACGG CTCCAAGCTC TTCTTTCGGC 
     4681 GCCTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//