LOCUS       RC200756                6831 bp    DNA     circular  SYN 
DEFINITION  RNPEP (Myc-DDK-tagged)-Human arginyl aminopeptidase (aminopeptidase B) (RNPEP)
ACCESSION   NM_020216
VERSION     NM_020216.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2988..3079 
                     /label="Myc-DDK"  
     misc_feature    3109..3694 
                     /label="PolyA signal"  
     misc_feature    3166..3185 
                     /label="Restriction Enzyme"  
     misc_feature    3970..4642 
                     /label="ColE1"  
     misc_feature    5072..5866 
                     /label="Kan/Neo"  
     misc_feature    5901..6203 
                     /label="SV40 ori"  
     misc_feature    6289..6766 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2970..2976 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2970 
                     /label="RNPEP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGGC GAGCATTCCC CCGGCAGCGG CGCGGCCCGG CGGCCGCTGC 
     1081 ACTCCGCGCA GGCTGTGGAC GTGGCCTCGG CCTCCAACTT CCGGGCCTTT GAGCTGCTGC 
     1141 ACTTGCACCT GGACCTGCGG GCTGAGTTCG GGCCTCCAGG GCCCGGCGCA GGGAGCCGGG 
     1201 GGCTGAGCGG CACCGCGGTC CTGGACCTGC GCTGCCTGGA GCCCGAGGGC GCCGCCGAGC 
     1261 TGCGGCTGGA CTCGCACCCG TGCCTGGAGG TGACGGCGGC GGCGCTGCGG CGGGAGCGGC 
     1321 CCGGCTCGGA GGAGCCGCCT GCGGAGCCCG TGAGCTTCTA CACGCAGCCC TTCTCGCACT 
     1381 ATGGCCAGGC CCTGTGCGTG TCCTTCCCGC AGCCCTGCCG CGCCGCCGAG CGCCTCCAGG 
     1441 TGCTGCTCAC CTACCGCGTC GGGGAGGGAC CCGGGGTTTG CTGGTTGGCT CCCGAGCAGA 
     1501 CAGCAGGAAA GAAGAAGCCC TTCGTGTACA CCCAGGGCCA GGCTGTCCTA AACCGGGCCT 
     1561 TCTTCCCTTG CTTCGACACG CCTGCTGTTA AATACAAGTA TTCAGCTCTT ATTGAGGTCC 
     1621 CAGATGGCTT CACAGCTGTG ATGAGTGCTA GCACCTGGGA GAAGAGAGGT CCAAATAAGT 
     1681 TCTTCTTCCA GATGTGTCAG CCCATCCCCT CCTATCTGAT AGCTTTGGCC ATCGGAGATC 
     1741 TGGTTTCGGC TGAAGTTGGA CCCAGGAGCC GGGTGTGGGC TGAGCCCTGC CTGATTGATG 
     1801 CTGCCAAGGA GGAGTACAAC GGGGTGATAG AAGAATTTTT GGCAACAGGA GAGAAGCTTT 
     1861 TTGGACCTTA TGTTTGGGGA AGGTATGACT TGCTCTTCAT GCCACCGTCC TTTCCATTTG 
     1921 GAGGAATGGA GAACCCTTGT CTGACCTTTG TCACCCCCTG CCTGCTAGCT GGGGACCGCT 
     1981 CCTTGGCAGA TGTCATCATC CATGAGATCT CCCACAGTTG GTTTGGGAAC CTGGTCACCA 
     2041 ACGCCAACTG GGGTGAATTC TGGCTCAATG AAGGTTTCAC CATGTACGCC CAGAGGAGGA 
     2101 TCTCCACCAT CCTCTTTGGC GCTGCGTACA CCTGCTTGGA GGCTGCAACG GGGCGGGCTC 
     2161 TGCTGCGTCA ACACATGGAC ATCACTGGAG AGGAAAACCC ACTCAACAAG CTCCGCGTGA 
     2221 AGATTGAACC AGGCGTTGAC CCGGACGACA CCTATAATGA GACCCCCTAC GAGAAAGGTT 
     2281 TCTGCTTTGT CTCATACCTG GCCCACTTGG TGGGTGATCA GGATCAGTTT GACAGTTTTC 
     2341 TCAAGGCCTA TGTGCATGAA TTCAAATTCC GAAGCATCTT AGCCGATGAC TTTCTGGACT 
     2401 TCTACTTGGA ATATTTCCCT GAGCTTAAGA AAAAGAGAGT GGATATCATT CCAGGTTTTG 
     2461 AGTTTGATCG ATGGCTGAAT ACCCCCGGCT GGCCCCCGTA CCTCCCTGAT CTCTCCCCTG 
     2521 GGGACTCACT CATGAAGCCT GCTGAAGAGC TAGCCCAACT GTGGGCAGCC GAGGAGCTGG 
     2581 ACATGAAGGC CATTGAAGCC GTGGCCATCT CTCCCTGGAA GACCTACCAG CTGGTCTACT 
     2641 TCCTGGATAA GATCCTCCAG AAATCCCCTC TCCCTCCTGG GAATGTGAAA AAACTTGGAG 
     2701 ACACATACCC AAGTATCTCA AATGCCCGGA ATGCAGAGCT CCGGCTGCGA TGGGGCCAAA 
     2761 TCGTCCTTAA GAACGACCAC CAGGAAGATT TCTGGAAAGT GAAGGAGTTC CTGCATAACC 
     2821 AGGGGAAGCA GAAGTATACA CTTCCGCTGT ACCACGCAAT GATGGGTGGC AGTGAGGTGG 
     2881 CCCAGACCCT CGCCAAGGAG ACTTTTGCAT CCACCGCCTC CCAGCTCCAC AGCAATGTTG 
     2941 TCAACTATGT CCAGCAGATC GTGGCACCCA AGGGCAGTAC GCGTACGCGG CCGCTCGAGC 
     3001 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3061 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3121 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3181 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3241 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3301 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3361 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3421 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3481 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3541 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3601 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3661 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3721 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3781 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3841 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3901 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     3961 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4021 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4081 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4141 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4201 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4261 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4321 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4381 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4441 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4501 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4561 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4621 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4681 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4741 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4801 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4861 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4921 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     4981 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5041 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5101 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5161 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5221 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5281 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5341 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5401 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5461 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5521 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5581 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5641 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5701 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5761 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5821 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5881 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     5941 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6001 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6061 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6121 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6181 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6241 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6301 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6361 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6421 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6481 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6541 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6601 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6661 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6721 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6781 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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