LOCUS       RC200731L2                9432 bp    DNA     circular  SYN 
DEFINITION  ITGB5 (Myc-DDK-tagged)-Human integrin, beta 5 (ITGB5)
ACCESSION   NM_002213
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7723..7903 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8631..9290 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7951..8570 
                     /label="ColE1"  
     misc_feature    6598..6618 
                     /label="LR50"  
     misc_feature    5738..6463 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6607..7198 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5717..5723 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5717 
                     /label="ITGB5"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCGCGGGCC CCGGCGCCGC TGTACGCCTG 
     3361 CCTCCTGGGG CTCTGCGCGC TCCTGCCCCG GCTCGCAGGT CTCAACATAT GCACTAGTGG 
     3421 AAGTGCCACC TCATGTGAAG AATGTCTGCT AATCCACCCA AAATGTGCCT GGTGCTCCAA 
     3481 AGAGGACTTC GGAAGCCCAC GGTCCATCAC CTCTCGGTGT GATCTGAGGG CAAACCTTGT 
     3541 CAAAAATGGC TGTGGAGGTG AGATAGAGAG CCCAGCCAGC AGCTTCCATG TCCTGAGGAG 
     3601 CCTGCCCCTC AGCAGCAAGG GTTCGGGCTC TGCAGGCTGG GACGTCATTC AGATGACACC 
     3661 ACAGGAGATT GCCGTGAACC TCCGGCCCGG TGACAAGACC ACCTTCCAGC TACAGGTTCG 
     3721 CCAGGTGGAG GACTATCCTG TGGACCTGTA CTACCTGATG GACCTCTCCC TGTCCATGAA 
     3781 GGATGACTTG GACAATATCC GGAGCCTGGG CACCAAACTC GCGGAGGAGA TGAGGAAGCT 
     3841 CACCAGCAAC TTCCGGTTGG GATTTGGGTC TTTTGTTGAT AAGGACATCT CTCCTTTCTC 
     3901 CTACACGGCA CCGAGGTACC AGACCAATCC GTGCATTGGT TACAAGTTGT TTCCAAATTG 
     3961 CGTCCCCTCC TTTGGGTTCC GCCATCTGCT GCCTCTCACA GACAGAGTGG ACAGCTTCAA 
     4021 TGAGGAAGTT CGGAAACAGA GGGTGTCCCG GAACCGAGAT GCCCCTGAGG GGGGCTTTGA 
     4081 TGCAGTACTC CAGGCAGCCG TCTGCAAGGA GAAGATTGGC TGGCGAAAGG ATGCACTGCA 
     4141 TTTGCTGGTG TTCACAACAG ATGATGTGCC CCACATCGCA TTGGATGGAA AATTGGGAGG 
     4201 CCTGGTGCAG CCACACGATG GCCAGTGCCA CCTGAACGAG GCCAACGAGT ACACTGCATC 
     4261 CAACCAGATG GACTATCCAT CCCTTGCCTT GCTTGGAGAG AAATTGGCAG AGAACAACAT 
     4321 CAACCTCATC TTTGCAGTGA CAAAAAACCA TTATATGCTG TACAAGAATT TTACAGCCCT 
     4381 GATACCTGGA ACAACGGTGG AGATTTTAGA TGGAGACTCC AAAAATATTA TTCAACTGAT 
     4441 TATTAATGCA TACAATAGTA TCCGGTCTAA AGTGGAGTTG TCAGTCTGGG ATCAGCCTGA 
     4501 GGATCTTAAT CTCTTCTTTA CTGCTACCTG CCAAGATGGG GTATCCTATC CTGGTCAGAG 
     4561 GAAGTGTGAG GGTCTGAAGA TTGGGGACAC GGCATCTTTT GAAGTATCAT TGGAGGCCCG 
     4621 AAGCTGTCCC AGCAGACACA CGGAGCATGT GTTTGCCCTG CGGCCGGTGG GATTCCGGGA 
     4681 CAGCCTGGAG GTGGGGGTCA CCTACAACTG CACGTGCGGC TGCAGCGTGG GGCTGGAACC 
     4741 CAACAGTGCC AGGTGCAACG GGAGCGGGAC CTATGTCTGC GGCCTGTGTG AGTGCAGCCC 
     4801 CGGCTACCTG GGCACCAGGT GCGAGTGCCA GGATGGGGAG AACCAGAGCG TGTACCAGAA 
     4861 CCTGTGCCGG GAGGCAGAGG GCAAGCCACT GTGCAGCGGG CGTGGGGACT GCAGCTGCAA 
     4921 CCAGTGCTCC TGCTTCGAGA GCGAGTTCGG CAAGATCTAT GGGCCTTTCT GTGAGTGCGA 
     4981 CAACTTCTCC TGTGCCAGGA ACAAGGGAGT CCTCTGCTCA GGCCATGGCG AGTGTCACTG 
     5041 CGGGGAATGC AAGTGCCATG CAGGTTACAT CGGGGACAAC TGTAACTGCT CGACAGACAT 
     5101 CAGCACATGC CGGGGCAGAG ATGGCCAGAT CTGCAGCGAG CGTGGGCACT GTCTCTGTGG 
     5161 GCAGTGCCAA TGCACGGAGC CGGGGGCCTT TGGGGAGATG TGTGAGAAGT GCCCCACCTG 
     5221 CCCGGATGCA TGCAGCACCA AGAGAGATTG CGTCGAGTGC CTGCTGCTCC ACTCTGGGAA 
     5281 ACCTGACAAC CAGACCTGCC ACAGCCTATG CAGGGATGAG GTGATCACAT GGGTGGACAC 
     5341 CATCGTGAAA GATGACCAGG AGGCTGTGCT ATGTTTCTAC AAAACCGCCA AGGACTGCGT 
     5401 CATGATGTTC ACCTATGTGG AGCTCCCCAG TGGGAAGTCC AACCTGACCG TCCTCAGGGA 
     5461 GCCAGAGTGT GGAAACACCC CCAACGCCAT GACCATCCTC CTGGCTGTGG TCGGTAGCAT 
     5521 CCTCCTTGTT GGGCTTGCAC TCCTGGCTAT CTGGAAGCTG CTTGTCACCA TCCACGACCG 
     5581 GAGGGAGTTT GCAAAGTTTC AGAGCGAGCG ATCCAGGGCC CGCTATGAAA TGGCTTCAAA 
     5641 TCCATTATAC AGAAAGCCTA TCTCCACGCA CACTGTGGAC TTCACCTTCA ACAAGTTCAA 
     5701 CAAATCCTAC AATGGCACTG TGGACACGCG TACGCGGCCG CTCGAGATGA GCGGGGGCGA 
     5761 GGAGCTGTTC GCCGGCATCG TGCCCGTGCT GATCGAGCTG GACGGCGACG TGCACGGCCA 
     5821 CAAGTTCAGC GTGCGCGGCG AGGGCGAGGG CGACGCCGAC TACGGCAAGC TGGAGATCAA 
     5881 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTGGTGA CCACCCTCTG 
     5941 CTACGGCATC CAGTGCTTCG CCCGCTACCC CGAGCACATG AAGATGAACG ACTTCTTCAA 
     6001 GAGCGCCATG CCCGAGGGCT ACATCCAGGA GCGCACCATC CAGTTCCAGG ACGACGGCAA 
     6061 GTACAAGACC CGCGGCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT 
     6121 GAAGGGCAAG GACTTCAAGG AGGACGGCAA CATCCTGGGC CACAAGCTGG AGTACAGCTT 
     6181 CAACAGCCAC AACGTGTACA TCCGCCCCGA CAAGGCCAAC AACGGCCTGG AGGCTAACTT 
     6241 CAAGACCCGC CACAACATCG AGGGCGGCGG CGTGCAGCTG GCCGACCACT ACCAGACCAA 
     6301 CGTGCCCCTG GGCGACGGCC CCGTGCTGAT CCCCATCAAC CACTACCTGA GCACTCAGAC 
     6361 CAAGATCAGC AAGGACCGCA ACGAGGCCCG CGACCACATG GTGCTCCTGG AGTCCTTCAG 
     6421 CGCCTGCTGC CACACCCACG GCATGGACGA GCTGTACAGG TCCGGACTCA GATAAGTTTA 
     6481 AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG 
     6541 CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT 
     6601 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 
     6661 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 
     6721 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 
     6781 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 
     6841 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 
     6901 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 
     6961 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 
     7021 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 
     7081 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 
     7141 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 
     7201 CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG 
     7261 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT 
     7321 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 
     7381 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 
     7441 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA 
     7501 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT 
     7561 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA 
     7621 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA 
     7681 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT 
     7741 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 
     7801 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 
     7861 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG 
     7921 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     7981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     8041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     8101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     8161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     8221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     8281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     8341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     8401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     8461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     8521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     8581 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT 
     8641 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 
     8701 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 
     8761 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 
     8821 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 
     8881 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 
     8941 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 
     9001 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 
     9061 CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 
     9121 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     9181 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 
     9241 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA 
     9301 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     9361 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     9421 GTGCCACCTG AC
//