LOCUS       RC200702L2                9375 bp    DNA     circular  SYN 
DEFINITION  PFKM (Myc-DDK-tagged)-Human phosphofructokinase, muscle (PFKM), transcript variant 4
ACCESSION   NM_000289
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7666..7846 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8574..9233 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7894..8513 
                     /label="ColE1"  
     misc_feature    6541..6561 
                     /label="LR50"  
     misc_feature    5681..6406 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6550..7141 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5660..5666 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5660 
                     /label="PFKM"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GACCCATGAA GAGCACCATG CAGCCAAAAC 
     3361 CCTGGGGATT GGCAAAGCCA TTGCTGTCTT AACCTCTGGT GGAGATGCCC AAGGTATGAA 
     3421 TGCTGCTGTC AGGGCTGTGG TTCGAGTTGG TATCTTCACC GGTGCCCGTG TCTTCTTTGT 
     3481 CCATGAGGGT TATCAAGGCC TGGTGGATGG TGGAGATCAC ATCAAGGAAG CCACCTGGGA 
     3541 GAGCGTTTCG ATGATGCTTC AGCTGGGAGG CACGGTGATT GGAAGTGCCC GGTGCAAGGA 
     3601 CTTTCGGGAA CGAGAAGGAC GACTCCGAGC TGCCTACAAC CTGGTGAAGC GTGGGATCAC 
     3661 CAATCTCTGT GTCATTGGGG GTGATGGCAG CCTCACTGGG GCTGACACCT TCCGTTCTGA 
     3721 GTGGAGTGAC TTGTTGAGTG ACCTCCAGAA AGCAGGTAAG ATCACAGATG AGGAGGCTAC 
     3781 GAAGTCCAGC TACCTGAACA TTGTGGGCCT GGTTGGGTCA ATTGACAATG ACTTCTGTGG 
     3841 CACCGATATG ACCATTGGCA CTGACTCTGC CCTGCATCGG ATCATGGAAA TTGTAGATGC 
     3901 CATCACTACC ACTGCCCAGA GCCACCAGAG GACATTTGTG TTAGAAGTAA TGGGCCGCCA 
     3961 CTGTGGATAC CTGGCCCTTG TCACCTCTCT GTCCTGTGGG GCCGACTGGG TTTTTATTCC 
     4021 TGAATGTCCA CCAGATGACG ACTGGGAGGA ACACCTTTGT CGCCGACTCA GCGAGACAAG 
     4081 GACCCGTGGT TCTCGTCTCA ACATCATCAT TGTGGCTGAG GGTGCAATTG ACAAGAATGG 
     4141 AAAACCAATC ACCTCAGAAG ACATCAAGAA TCTGGTGGTT AAGCGTCTGG GATATGACAC 
     4201 CCGGGTTACT GTCTTGGGGC ATGTGCAGAG GGGTGGGACG CCATCAGCCT TTGACAGAAT 
     4261 TCTGGGCAGC AGGATGGGTG TGGAAGCAGT GATGGCACTT TTGGAGGGGA CCCCAGATAC 
     4321 CCCAGCCTGT GTAGTGAGCC TCTCTGGTAA CCAGGCTGTG CGCCTGCCCC TCATGGAATG 
     4381 TGTCCAGGTG ACCAAAGATG TGACCAAGGC CATGGATGAG AAGAAATTTG ACGAAGCCCT 
     4441 GAAGCTGAGA GGCCGGAGCT TCATGAACAA CTGGGAGGTG TACAAGCTTC TAGCTCATGT 
     4501 CAGACCCCCG GTATCTAAGA GTGGTTCGCA CACAGTGGCT GTGATGAACG TGGGGGCTCC 
     4561 GGCTGCAGGC ATGAATGCTG CTGTTCGCTC CACTGTGAGG ATTGGCCTTA TCCAGGGCAA 
     4621 CCGAGTGCTC GTTGTCCATG ATGGTTTCGA GGGCCTGGCC AAGGGGCAGA TAGAGGAAGC 
     4681 TGGCTGGAGC TATGTTGGGG GCTGGACTGG CCAAGGTGGC TCTAAACTTG GGACTAAAAG 
     4741 GACTCTACCC AAGAAGAGCT TTGAACAGAT CAGTGCCAAT ATAACTAAGT TTAACATTCA 
     4801 GGGCCTTGTC ATCATTGGGG GCTTTGAGGC TTACACAGGG GGCCTGGAAC TGATGGAGGG 
     4861 CAGGAAGCAG TTTGATGAGC TCTGCATCCC ATTTGTGGTC ATTCCTGCTA CAGTCTCCAA 
     4921 CAATGTCCCT GGCTCAGACT TCAGCGTTGG GGCTGACACA GCACTCAATA CTATCTGCAC 
     4981 AACCTGTGAC CGCATCAAGC AGTCAGCAGC TGGCACCAAG CGTCGGGTGT TTATCATTGA 
     5041 GACTATGGGT GGCTACTGTG GCTACCTGGC TACCATGGCT GGACTGGCAG CTGGGGCCGA 
     5101 TGCTGCCTAC ATTTTTGAGG AGCCCTTCAC CATTCGAGAC CTGCAGGCAA ATGTTGAACA 
     5161 TCTGGTGCAA AAGATGAAAA CAACTGTGAA AAGGGGCTTG GTGTTAAGGA ATGAAAAGTG 
     5221 CAATGAGAAC TATACCACTG ACTTCATTTT CAACCTGTAC TCTGAGGAGG GGAAGGGCAT 
     5281 CTTCGACAGC AGGAAGAATG TGCTTGGTCA CATGCAGCAG GGTGGGAGCC CAACCCCATT 
     5341 TGATAGGAAT TTTGCCACTA AGATGGGCGC CAAGGCTATG AACTGGATGT CTGGGAAAAT 
     5401 CAAAGAGAGT TACCGTAATG GGCGGATCTT TGCCAATACT CCAGATTCGG GCTGTGTTCT 
     5461 GGGGATGCGT AAGAGGGCTC TGGTCTTCCA ACCAGTGGCT GAGCTGAAGG ACCAGACAGA 
     5521 TTTTGAGCAT CGAATCCCCA AGGAACAGTG GTGGCTGAAA CTGAGGCCCA TCCTCAAAAT 
     5581 CCTAGCCAAG TACGAGATTG ACTTGGACAC TTCAGACCAT GCCCACCTGG AGCACATCAC 
     5641 CCGGAAGCGG TCCGGGGAAG CTGCCGTCAC GCGTACGCGG CCGCTCGAGA TGAGCGGGGG 
     5701 CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT GCTGATCGAG CTGGACGGCG ACGTGCACGG 
     5761 CCACAAGTTC AGCGTGCGCG GCGAGGGCGA GGGCGACGCC GACTACGGCA AGCTGGAGAT 
     5821 CAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTGG TGACCACCCT 
     5881 CTGCTACGGC ATCCAGTGCT TCGCCCGCTA CCCCGAGCAC ATGAAGATGA ACGACTTCTT 
     5941 CAAGAGCGCC ATGCCCGAGG GCTACATCCA GGAGCGCACC ATCCAGTTCC AGGACGACGG 
     6001 CAAGTACAAG ACCCGCGGCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 
     6061 GCTGAAGGGC AAGGACTTCA AGGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAG 
     6121 CTTCAACAGC CACAACGTGT ACATCCGCCC CGACAAGGCC AACAACGGCC TGGAGGCTAA 
     6181 CTTCAAGACC CGCCACAACA TCGAGGGCGG CGGCGTGCAG CTGGCCGACC ACTACCAGAC 
     6241 CAACGTGCCC CTGGGCGACG GCCCCGTGCT GATCCCCATC AACCACTACC TGAGCACTCA 
     6301 GACCAAGATC AGCAAGGACC GCAACGAGGC CCGCGACCAC ATGGTGCTCC TGGAGTCCTT 
     6361 CAGCGCCTGC TGCCACACCC ACGGCATGGA CGAGCTGTAC AGGTCCGGAC TCAGATAAGT 
     6421 TTAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA TAACTTCGTA 
     6481 TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA GGTACAATTC 
     6541 GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 
     6601 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 
     6661 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 
     6721 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 
     6781 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 
     6841 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 
     6901 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG 
     6961 TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 
     7021 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 
     7081 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 
     7141 TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG CAATCACAAG 
     7201 TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA 
     7261 GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 
     7321 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 
     7381 AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTGGCA 
     7441 GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT 
     7501 AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA CCCGCTTGTT 
     7561 ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG AGTGGAGGTT 
     7621 TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT GTACTGGGTC 
     7681 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT 
     7741 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA 
     7801 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGCAT 
     7861 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     7921 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     7981 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     8041 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     8101 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     8161 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     8221 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     8281 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     8341 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     8401 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     8461 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     8521 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     8581 GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG 
     8641 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG 
     8701 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG 
     8761 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA 
     8821 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC 
     8881 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC 
     8941 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC 
     9001 TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG 
     9061 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA 
     9121 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA 
     9181 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC 
     9241 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     9301 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     9361 AAAGTGCCAC CTGAC
//