LOCUS       RC200609                7212 bp    DNA     circular  SYN 
DEFINITION  HGS (Myc-DDK-tagged)-Human hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)
ACCESSION   NM_004712
VERSION     NM_004712.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3369..3460 
                     /label="Myc-DDK"  
     misc_feature    3490..4075 
                     /label="PolyA signal"  
     misc_feature    3547..3566 
                     /label="Restriction Enzyme"  
     misc_feature    4351..5023 
                     /label="ColE1"  
     misc_feature    5453..6247 
                     /label="Kan/Neo"  
     misc_feature    6282..6584 
                     /label="SV40 ori"  
     misc_feature    6670..7147 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3351..3357 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3351 
                     /label="HGS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGAGGC AGCGGCACCT TCGAGCGTCT CCTAGACAAG GCGACCAGCC 
     1081 AGCTCCTGTT GGAGACAGAT TGGGAGTCCA TTTTGCAGAT CTGCGACCTG ATCCGCCAAG 
     1141 GGGACACACA AGCAAAATAT GCTGTGAATT CCATCAAGAA GAAAGTCAAC GACAAGAACC 
     1201 CACACGTCGC CTTGTATGCC CTGGAGGTCA TGGAATCTGT GGTAAAGAAC TGTGGCCAGA 
     1261 CAGTTCATGA TGAGGTGGCC AACAAGCAGA CCATGGAGGA GCTGAAGGAC CTGCTGAAGA 
     1321 GACAAGTGGA GGTAAACGTC CGTAACAAGA TCCTGTACCT GATCCAGGCC TGGGCGCATG 
     1381 CCTTCCGGAA CGAGCCCAAG TACAAGGTGG TCCAGGACAC CTACCAGATC ATGAAGGTGG 
     1441 AGGGGCACGT CTTTCCAGAA TTCAAAGAGA GCGATGCCAT GTTTGCTGCC GAGAGAGCCC 
     1501 CAGACTGGGT GGACGCTGAG GAATGCCACC GCTGCAGGGT GCAGTTCGGG GTGATGACCC 
     1561 GTAAGCACCA CTGCCGGGCG TGTGGGCAGA TATTCTGTGG AAAGTGTTCT TCCAAGTACT 
     1621 CCACCATCCC CAAGTTTGGC ATCGAGAAGG AGGTGCGCGT GTGTGAGCCC TGCTACGAGC 
     1681 AGCTGAACAG GAAAGCGGAG GGAAAGGCCA CTTCCACCAC TGAGCTGCCC CCCGAGTACC 
     1741 TGACCAGCCC CCTGTCTCAG CAGTCCCAGC TGCCCCCCAA GAGGGACGAG ACGGCCCTGC 
     1801 AGGAGGAGGA GGAGCTGCAG CTGGCCCTGG CGCTGTCACA GTCAGAGGCG GAGGAGAAGG 
     1861 AGAGGCTGAG ACAGAAGTCC ACGTACACTT CGTACCCCAA GGCGGAGCCC ATGCCCTCGG 
     1921 CCTCCTCAGC GCCCCCCGCC AGCAGCCTGT ACTCTTCACC TGTGAACTCG TCGGCGCCTC 
     1981 TGGCTGAGGA CATCGACCCT GAGCTCGCAC GGTATCTCAA CCGGAACTAC TGGGAGAAGA 
     2041 AGCAGGAGGA GGCTCGCAAG AGCCCCACGC CATCTGCGCC CGTGCCCCTG ACGGAGCCGG 
     2101 CTGCACAGCC TGGGGAAGGG CACGCAGCCC CCACCAACGT GGTGGAGAAC CCCCTCCCGG 
     2161 AGACAGACTC TCAGCCCATT CCTCCCTCTG GTGGCCCCTT TAGTGAGCCA CAGTTCCACA 
     2221 ATGGCGAGTC TGAGGAGAGC CACGAGCAGT TCCTGAAGGC GCTGCAGAAC GCCGTCACCA 
     2281 CCTTCGTGAA CCGCATGAAG AGTAACCACA TGCGGGGCCG CAGCATCACC AATGACTCGG 
     2341 CCGTGCTCTC ACTCTTCCAG TCCATCAACG GCATGCACCC GCAGCTGCTG GAGCTGCTCA 
     2401 ACCAGCTGGA CGAGCGCAGG CTGTACTATG AGGGGCTGCA GGACAAGCTG GCACAGATCC 
     2461 GCGATGCCCG GGGGGCGCTG AGTGCCCTGC GCGAAGAGCA CCGGGAGAAG CTTCGCCGGG 
     2521 CAGCCGAGGA GGCAGAGCGC CAGCGCCAGA TCCAGCTGGC CCAGAAGCTG GAGATAATGC 
     2581 GGCAGAAGAA GCAGGAGTAC CTGGAGGTGC AGAGGCAGCT GGCCATCCAG CGCCTGCAGG 
     2641 AGCAGGAGAA GGAGCGGCAG ATGCGGCTGG AGCAGCAGAA GCAGACGGTC CAGATGCGCG 
     2701 CGCAGATGCC CGCCTTCCCC CTGCCCTACG CCCAGCTCCA GGCCATGCCC GCAGCCGGAG 
     2761 GTGTGCTCTA CCAGCCCTCG GGACCAGCCA GCTTCCCCAG CACCTTCAGC CCTGCCGGCT 
     2821 CGGTGGAGGG CTCCCCAATG CACGGCGTGT ACATGAGCCA GCCGGCCCCT GCCGCTGGCC 
     2881 CCTACCCCAG CATGCCCAGC ACTGCGGCTG ATCCCAGCAT GGTGAGTGCC TACATGTACC 
     2941 CAGCAGGGGC CACTGGGGCG CAGGCGGCCC CCCAGGCCCA GGCCGGACCC ACCGCCAGCC 
     3001 CCGCTTACTC ATCCTACCAG CCTACTCCCA CAGCGGGCTA CCAGAACGTG GCCTCCCAGG 
     3061 CCCCACAGAG CCTCCCGGCC ATCTCTCAGC CTCCGCAGTC CAGCACCATG GGCTACATGG 
     3121 GGAGCCAGTC AGTCTCCATG GGCTACCAGC CTTACAACAT GCAGAATCTC ATGACCACCC 
     3181 TCCCAAGCCA GGATGCGTCT CTGCCACCCC AGCAGCCCTA CATCGCGGGG CAGCAGCCCA 
     3241 TGTACCAGCA GATGGCACCC TCTGGCGGTC CCCCCCAGCA GCAGCCCCCC GTGGCCCAGC 
     3301 AACCGCAGGC ACAGGGGCCG CCGGCACAGG GCAGCGAGGC CCAGCTCATT TCATTCGACA 
     3361 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3421 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3481 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3541 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3601 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3661 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3721 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3781 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3841 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3901 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3961 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4021 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4081 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4141 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4201 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4261 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4321 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4381 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4441 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4501 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4561 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4621 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4681 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4741 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4801 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4861 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4921 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4981 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5041 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5101 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5161 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5221 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5281 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5341 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5401 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5461 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5521 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5581 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5641 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5701 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5761 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5821 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5881 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5941 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6001 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6061 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6121 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6181 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6241 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6301 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6361 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6421 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6481 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6541 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6601 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6661 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6721 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6781 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6841 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6901 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7021 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7081 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7141 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7201 AACGCGAATT TT
//