LOCUS       RC200573                8475 bp    DNA     circular  SYN 
DEFINITION  PAN2 (Myc-DDK-tagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 3
ACCESSION   NM_014871
VERSION     NM_014871.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4614..4620 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4614 
                     /label="PAN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGAG GGTCTGGACC CTGGACTGGC AGAATATGCC CCAGCCATGC 
     1081 ATTCTGCCCT GGACCCTGTC TTGGATGCCC ACCTGAACCC AAATCTGCTA CAGAATGTGG 
     1141 AGCTGGACCC AGAGGGAGTG GCCTTGGAGG CTCTTCCCGT CCAGGAATCA GTGCACATAA 
     1201 TGGAAGGTGT CTACTCTGAA TTGCACAGCG TGGTGGCTGA AGTGGGTGTA CCTGTTTCCG 
     1261 TCTCCCACTT TGACTTGCAC GAGGAGATGC TGTGGGTGGG GAGCCACGGG GGCCATGCCA 
     1321 CTTCATTTTT TGGCCCAGCC TTGGAGCGCT ACTCATCCTT TCAAGTCAAT GGCAGTGATG 
     1381 ATATTCGGCA GATCCAGAGC CTGGAGAATG GTATCCTTTT TCTCACCAAG AACAACCTCA 
     1441 AGTATATGGC CCGTGGGGGC CTCATTATAT TTGATTACCT GCTGGATGAG AATGAGGATA 
     1501 TGCACAGTCT CCTACTGACT GACAGCAGCA CTCTACTCGT TGGTGGGCTG CAGAATCACA 
     1561 TACTAGAGAT TGATCTTAAC ACTGTCCAGG AGACTCAGAA GTATGCAGTA GAGACGCCTG 
     1621 GAGTCACCAT CATGAGACAG ACAAATCGCT TCTTCTTCTG CGGCCACACG TCTGGCAAGG 
     1681 TTTCCCTGAG AGACCTCCGT ACTTTTAAGG TGGAACATGA GTTTGATGCC TTCTCAGGAA 
     1741 GTCTGTCAGA CTTTGATGTG CATGGCAACC TGCTAGCTGC CTGTGGCTTC TCCAGCCGCC 
     1801 TCACTGGCCT GGCCTGCGAC CGTTTCCTCA AGGTGTATGA TTTGCGCATG ATGCGTGCCA 
     1861 TCACACCACT TCAAGTACAT GTGGATCCTG CCTTCTTGCG CTTCATTCCT ACATATACTT 
     1921 CTCGTCTTGC TATCATCTCT CAGTCAGGGC AGTGCCAATT CTGTGAACCC ACAGGCCTGG 
     1981 CCAACCCAGC CGATATCTTT CATGTGAATC CTGTGGGGCC TCTGCTAATG ACATTTGATG 
     2041 TGTCAGCCAG CAAGCAGGCT CTGGCCTTTG GGGATTCTGA GGGCTGTGTG CACCTCTGGA 
     2101 CTGATTCCCC GGAGCCTTCC TTCAACCCCT ACTCCCGTGA GACTGAGTTT GCTTTGCCGT 
     2161 GTCTCGTGGA CTCACTGCCT CCTCTGGACT GGAGCCAGGA CCTGCTGCCT CTTTCCCTCA 
     2221 TCCCTGTCCC ACTCACCACT GACACACTTC TCTCTGATTG GCCTGCTGCC AACTCTGCTC 
     2281 CAGCTCCCAG GCGAGCACCA CCCGTGGATG CAGAGATTCT GCGCACCATG AAGAAGGTGG 
     2341 GCTTCATTGG CTATGCGCCC AATCCCCGCA CCAGGCTGCG CAATCAGATA CCCTACAGAC 
     2401 TCAAGGAGTC AGACAGTGAA TTTGACAGCT TCAGCCAGGT CACTGAGTCA CCAGTAGGAC 
     2461 GAGAAGAGGA ACCACATCTC CACATGGTTT CTAAGAAATA CCGCAAGGTG ACCATCAAAT 
     2521 ATTCCAAGCT AGGGCTGGAG GACTTTGACT TCAAACACTA CAATAAGACC TTGTTTGCTG 
     2581 GATTAGAGCC CCACATTCCC AACGCCTACT GTAACTGCAT GATCCAGGTG CTCTATTTCC 
     2641 TGGAGCCTGT ACGCTGTCTA ATTCAAAACC ACCTTTGCCA GAAGGAGTTC TGTCTGGCAT 
     2701 GTGAGCTGGG CTTCCTGTTT CACATGTTGG ACCTCTCTCG TGGTGACCCT TGCCAGGGCA 
     2761 ATAATTTTCT TCGGGCATTC CGTACTATTC CTGAGGCCTC AGCCCTCGGT CTAATCCTGG 
     2821 CTGACTCAGA TGAGGCCTCA GGCAAGGGCA ATCTGGCCAG GCTCATTCAG AGGTGGAATC 
     2881 GCTTCATTCT CACTCAACTG CATCAAGATA TGCAGGAGCT GGAAATACCA CAGGCTTATC 
     2941 GAGGTGCTGG AGGCAGCAGC TTTTGCTCAT CGGGGGACTC TGTTATTGGG CAGCTCTTCA 
     3001 GCTGTGAGAT GGAGAACTGC AGCCTCTGCC GCTGTGGCAG TGAGACCGTG CGAGCCTCAT 
     3061 CCACTCTGCT TTTCACACTC TCCTACCCTG ATGATAAAAC TGGGAAGAAC TATGACTTTG 
     3121 CTCAGGTGCT GAAGCGAAGC ATCTGCCTGG ACCAGAATAC ACAGGCCTGG TGTGACACCT 
     3181 GTGAAAAGTA CCAGCCCACG ATTCAGACCC GCAACATCCG CCATCTGCCA GATATTCTTG 
     3241 TCATCAATTG TGAGGTGAAC AGCTCAAAAG AGGCTGATTT CTGGAGAATG CAGGCTGAGG 
     3301 TTGCCTTCAA GATGGCAGTA AAGAAACACG GTGGGGAAAT CTCCAAGAAC AAGGAATTTG 
     3361 CTTTGGCTGA TTGGAAGGAA CTAGGGAGTC CAGAGGGTGT GCTGGTGTGT CCCTCCATTG 
     3421 AGGAGTTGAA GAACGTCTGG CTTCCTTTCT CCATTCGCAT GAAGATGACC AAAAACAAAG 
     3481 GGCTGGATGT TTGCAATTGG ACTGATGGGG ATGAGATGCA GTGGGGCCCA GCCAGGGCAG 
     3541 AGGAGGAGCA TGGTGTCTAT GTGTATGACC TGATGGCTAC TGTGGTACAC ATCCTGGACT 
     3601 CACGCACAGG GGGCAGCCTG GTGGCTCACA TCAAAGTTGG AGAGACCTAC CACCAGCGCA 
     3661 AGGAGGGCGT TACTCACCAG CAGTGGTATC TGTTCAATGA CTTTCTTATT GAACCTATTG 
     3721 ATAAGCATGA AGCTGTGCAG TTTGACATGA ATTGGAAAGT ACCTGCAATC CTTTATTATG 
     3781 TCAAACGGAA TCTCAATTCC AGATACAACC TGAACATCAA GAACCCTATT GAGGCAAGTG 
     3841 TCTTGCTGGC TGAAGCCTCG CTGGCACGGA AGCAGCGGAA AACACATACT ACCTTTATTC 
     3901 CACTGATGCT GAATGAGATG CCACAGATTG GGGACCTGGT GGGTCTGGAT GCTGAGTTTG 
     3961 TCACCCTTAA TGAGGAGGAA GCAGAGTTAC GCAGTGATGG TACCAAGTCT ACCATTAAAC 
     4021 CAAGCCAGAT GTCAGTAGCC AGGATTACCT GTGTTCGGGG CCAGGGACCC AATGAGGGTA 
     4081 TCCCCTTCAT TGATGACTAC ATCTCTACCC AGGAGCAGGT GGTGGATTAC TTGACTCAAT 
     4141 ACTCGGGTAT AAAGCCTGGT GACCTCGATG CCAAAATTTC CTCCAAGCAC CTAACAACTC 
     4201 TCAAGTCTAC CTACTTAAAG CTTCGTTTTC TCATTGACAT TGGAGTCAAG TTTGTGGGTC 
     4261 ATGGCCTGCA GAAGGACTTC CGGGTCATCA ACCTGATGGT GCCCAAGGAC CAAGTCCTTG 
     4321 ACACTGTCTA CCTGTTCCAT ATGCCCCGAA AACGAATGAT TTCCCTGCGA TTCCTTGCTT 
     4381 GGTACTTTCT GGACCTGAAG ATTCAAGGGG AAACCCATGA CAGTATTGAG GATGCCCGCA 
     4441 CAGCCCTTCA GCTGTACCGA AAGTATCTGG AGCTAAGCAA AAATGGCACT GAGCCTGAGT 
     4501 CTTTCCACAA GGTGCTCAAG GGTCTTTATG AGAAGGGCAG AAAGATGGAC TGGAAGGTGC 
     4561 CTGAGCCTGA GGGCCAAACA AGTCCCAAGA ATGCAGCTGT CTTCTCCTCA GTGCTGGCGC 
     4621 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//