LOCUS       RC200508                8184 bp    DNA     circular  SYN 
DEFINITION  ACLY (Myc-DDK-tagged)-Human ATP citrate lyase (ACLY), transcript variant 1
ACCESSION   NM_001096
VERSION     NM_001096.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4341..4432 
                     /label="Myc-DDK"  
     misc_feature    4462..5047 
                     /label="PolyA signal"  
     misc_feature    4519..4538 
                     /label="Restriction Enzyme"  
     misc_feature    5323..5995 
                     /label="ColE1"  
     misc_feature    6425..7219 
                     /label="Kan/Neo"  
     misc_feature    7254..7556 
                     /label="SV40 ori"  
     misc_feature    7642..8119 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4323..4329 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4323 
                     /label="ACLY"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCAAG GCAATTTCAG AGCAGACGGG CAAAGAACTC CTTTACAAGT 
     1081 TCATCTGTAC CACCTCAGCC ATCCAGAATC GGTTCAAGTA TGCTCGGGTC ACTCCTGACA 
     1141 CAGACTGGGC CCGCTTGCTG CAGGACCACC CCTGGCTGCT CAGCCAGAAC TTGGTAGTCA 
     1201 AGCCAGACCA GCTGATCAAA CGTCGTGGAA AACTTGGTCT CGTTGGGGTC AACCTCACTC 
     1261 TGGATGGGGT CAAGTCCTGG CTGAAGCCAC GGCTGGGACA GGAAGCCACA GTTGGCAAGG 
     1321 CCACAGGCTT CCTCAAGAAC TTTCTGATCG AGCCCTTCGT CCCCCACAGT CAGGCTGAGG 
     1381 AGTTCTATGT CTGCATCTAT GCCACCCGAG AAGGGGACTA CGTCCTGTTC CACCACGAGG 
     1441 GGGGTGTGGA CGTGGGTGAT GTGGACGCCA AGGCCCAGAA GCTGCTTGTT GGCGTGGATG 
     1501 AGAAACTGAA TCCTGAGGAC ATCAAAAAAC ACCTGTTGGT CCACGCCCCT GAAGACAAGA 
     1561 AAGAAATTCT GGCCAGTTTT ATCTCCGGCC TCTTCAATTT CTACGAGGAC TTGTACTTCA 
     1621 CCTACCTCGA GATCAATCCC CTTGTAGTGA CCAAAGATGG AGTCTATGTC CTTGACTTGG 
     1681 CGGCCAAGGT GGACGCCACT GCCGACTACA TCTGCAAAGT GAAGTGGGGT GACATCGAGT 
     1741 TCCCTCCCCC CTTCGGGCGG GAGGCATATC CAGAGGAAGC CTACATTGCA GACCTCGATG 
     1801 CCAAAAGTGG GGCAAGCCTG AAGCTGACCT TGCTGAACCC CAAAGGGAGG ATCTGGACCA 
     1861 TGGTGGCCGG GGGTGGCGCC TCTGTCGTGT ACAGCGATAC CATCTGTGAT CTAGGGGGTG 
     1921 TCAACGAGCT GGCAAACTAT GGGGAGTACT CAGGCGCCCC CAGCGAGCAG CAGACCTATG 
     1981 ACTATGCCAA GACTATCCTC TCCCTCATGA CCCGAGAGAA GCACCCAGAT GGCAAGATCC 
     2041 TCATCATTGG AGGCAGCATC GCAAACTTCA CCAACGTGGC TGCCACGTTC AAGGGCATCG 
     2101 TGAGAGCAAT TCGAGATTAC CAGGGCCCCC TGAAGGAGCA CGAAGTCACA ATCTTTGTCC 
     2161 GAAGAGGTGG CCCCAACTAT CAGGAGGGCT TACGGGTGAT GGGAGAAGTC GGGAAGACCA 
     2221 CTGGGATCCC CATCCATGTC TTTGGCACAG AGACTCACAT GACGGCCATT GTGGGCATGG 
     2281 CCCTGGGCCA CCGGCCCATC CCCAACCAGC CACCCACAGC GGCCCACACT GCAAACTTCC 
     2341 TCCTCAACGC CAGCGGGAGC ACATCGACGC CAGCCCCCAG CAGGACAGCA TCTTTTTCTG 
     2401 AGTCCAGGGC CGATGAGGTG GCGCCTGCAA AGAAGGCCAA GCCTGCCATG CCACAAGATT 
     2461 CAGTCCCAAG TCCAAGATCC CTGCAAGGAA AGAGCACCAC CCTCTTCAGC CGCCACACCA 
     2521 AGGCCATTGT GTGGGGCATG CAGACCCGGG CCGTGCAAGG CATGCTGGAC TTTGACTATG 
     2581 TCTGCTCCCG AGACGAGCCC TCAGTGGCTG CCATGGTCTA CCCTTTCACT GGGGACCACA 
     2641 AGCAGAAGTT TTACTGGGGG CACAAAGAGA TCCTGATCCC TGTCTTCAAG AACATGGCTG 
     2701 ATGCCATGAG GAAGCATCCG GAGGTAGATG TGCTCATCAA CTTTGCCTCT CTCCGCTCTG 
     2761 CCTATGACAG CACCATGGAG ACCATGAACT ATGCCCAGAT CCGGACCATC GCCATCATAG 
     2821 CTGAAGGCAT CCCTGAGGCC CTCACGAGAA AGCTGATCAA GAAGGCGGAC CAGAAGGGAG 
     2881 TGACCATCAT CGGACCTGCC ACTGTTGGAG GCATCAAGCC TGGGTGCTTT AAGATTGGCA 
     2941 ACACAGGTGG GATGCTGGAC AACATCCTGG CCTCCAAACT GTACCGCCCA GGCAGCGTGG 
     3001 CCTATGTCTC ACGTTCCGGA GGCATGTCCA ACGAGCTCAA CAATATCATC TCTCGGACCA 
     3061 CGGATGGCGT CTATGAGGGC GTGGCCATTG GTGGGGACAG GTACCCGGGC TCCACATTCA 
     3121 TGGATCATGT GTTACGCTAT CAGGACACTC CAGGAGTCAA AATGATTGTG GTTCTTGGAG 
     3181 AGATTGGGGG CACTGAGGAA TATAAGATTT GCCGGGGCAT CAAGGAGGGC CGCCTCACTA 
     3241 AGCCCATCGT CTGCTGGTGC ATCGGGACGT GTGCCACCAT GTTCTCCTCT GAGGTCCAGT 
     3301 TTGGCCATGC TGGAGCTTGT GCCAACCAGG CTTCTGAAAC TGCAGTAGCC AAGAACCAGG 
     3361 CTTTGAAGGA AGCAGGAGTG TTTGTGCCCC GGAGCTTTGA TGAGCTTGGA GAGATCATCC 
     3421 AGTCTGTATA CGAAGATCTC GTGGCCAATG GAGTCATTGT ACCTGCCCAG GAGGTGCCGC 
     3481 CCCCAACCGT GCCCATGGAC TACTCCTGGG CCAGGGAGCT TGGTTTGATC CGCAAACCTG 
     3541 CCTCGTTCAT GACCAGCATC TGCGATGAGC GAGGACAGGA GCTCATCTAC GCGGGCATGC 
     3601 CCATCACTGA GGTCTTCAAG GAAGAGATGG GCATTGGCGG GGTCCTCGGC CTCCTCTGGT 
     3661 TCCAGAAAAG GTTGCCTAAG TACTCTTGCC AGTTCATTGA GATGTGTCTG ATGGTGACAG 
     3721 CTGATCACGG GCCAGCCGTC TCTGGAGCCC ACAACACCAT CATTTGTGCG CGAGCTGGGA 
     3781 AAGACCTGGT CTCCAGCCTC ACCTCGGGGC TGCTCACCAT CGGGGATCGG TTTGGGGGTG 
     3841 CCTTGGATGC AGCAGCCAAG ATGTTCAGTA AAGCCTTTGA CAGTGGCATT ATCCCCATGG 
     3901 AGTTTGTGAA CAAGATGAAG AAGGAAGGGA AGCTGATCAT GGGCATTGGT CACCGAGTGA 
     3961 AGTCGATAAA CAACCCAGAC ATGCGAGTGC AGATCCTCAA AGATTACGTC AGGCAGCACT 
     4021 TCCCTGCCAC TCCTCTGCTC GATTATGCAC TGGAAGTAGA GAAGATTACC ACCTCGAAGA 
     4081 AGCCAAATCT TATCCTGAAT GTAGATGGTC TCATCGGAGT CGCATTTGTA GACATGCTTA 
     4141 GAAACTGTGG GTCCTTTACT CGGGAGGAAG CTGATGAATA TATTGACATT GGAGCCCTCA 
     4201 ATGGCATCTT TGTGCTGGGA AGGAGTATGG GGTTCATTGG ACACTATCTT GATCAGAAGA 
     4261 GGCTGAAGCA GGGGCTGTAT CGTCATCCGT GGGATGATAT TTCATATGTT CTTCCGGAAC 
     4321 ACATGAGCAT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4381 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4441 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4501 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4561 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4621 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4681 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4741 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4801 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4861 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4921 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4981 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5041 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5101 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5161 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5221 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5281 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5341 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5401 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5461 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5521 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5581 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5641 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5701 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5761 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5821 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5881 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5941 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6001 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6061 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6121 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6181 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6241 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6301 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6361 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6421 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6481 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6541 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6601 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6661 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6721 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6781 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6841 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6901 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6961 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7021 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7081 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7141 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7201 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7261 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7321 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7381 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7621 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7681 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7741 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7801 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7861 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7921 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7981 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8041 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8101 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8161 TAACAAAAAT TTAACGCGAA TTTT
//