LOCUS       RC200371                6723 bp    DNA     circular  SYN 
DEFINITION  DDX5 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5)
ACCESSION   NM_004396
VERSION     NM_004396.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2880..2971 
                     /label="Myc-DDK"  
     misc_feature    3001..3586 
                     /label="PolyA signal"  
     misc_feature    3058..3077 
                     /label="Restriction Enzyme"  
     misc_feature    3862..4534 
                     /label="ColE1"  
     misc_feature    4964..5758 
                     /label="Kan/Neo"  
     misc_feature    5793..6095 
                     /label="SV40 ori"  
     misc_feature    6181..6658 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2862..2868 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2862 
                     /label="DDX5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGGTTAT TCGAGTGACC GAGACCGCGG CCGGGACCGA GGGTTTGGTG 
     1081 CACCTCGATT TGGAGGAAGT AGGGCAGGGC CCTTATCTGG AAAGAAGTTT GGAAACCCTG 
     1141 GGGAGAAATT AGTTAAAAAG AAGTGGAATC TTGATGAGCT GCCTAAATTT GAGAAGAATT 
     1201 TTTATCAAGA GCACCCTGAT TTGGCTAGGC GCACAGCACA AGAGGTGGAA ACATACAGAA 
     1261 GAAGCAAGGA AATTACAGTT AGAGGTCACA ACTGCCCGAA GCCAGTTCTA AATTTTTATG 
     1321 AAGCCAATTT CCCTGCAAAT GTCATGGATG TTATTGCAAG ACAGAATTTC ACTGAACCCA 
     1381 CTGCTATTCA AGCTCAGGGA TGGCCAGTTG CTCTAAGTGG ATTGGATATG GTTGGAGTGG 
     1441 CACAGACTGG ATCTGGGAAA ACATTGTCTT ATTTGCTTCC TGCCATTGTC CACATCAATC 
     1501 ATCAGCCATT CCTAGAGAGA GGCGATGGGC CTATTTGTTT GGTGCTGGCA CCAACTCGGG 
     1561 AACTGGCCCA ACAGGTGCAG CAAGTAGCTG CTGAATATTG TAGAGCATGT CGCTTGAAGT 
     1621 CTACTTGTAT CTACGGTGGT GCTCCTAAGG GACCACAAAT ACGTGATTTG GAGAGAGGTG 
     1681 TGGAAATCTG TATTGCAACA CCTGGAAGAC TGATTGACTT TTTAGAGTGT GGAAAAACCA 
     1741 ATCTGAGAAG AACAACCTAC CTTGTCCTTG ATGAAGCAGA TAGAATGCTT GATATGGGCT 
     1801 TTGAACCCCA AATAAGGAAG ATTGTGGATC AAATAAGACC TGATAGGCAA ACTCTAATGT 
     1861 GGAGTGCGAC TTGGCCAAAA GAAGTAAGAC AGCTTGCTGA AGATTTCCTG AAAGACTATA 
     1921 TTCATATAAA CATTGGTGCA CTTGAACTGA GTGCAAACCA CAACATTCTT CAGATTGTGG 
     1981 ATGTGTGTCA TGACGTAGAA AAGGATGAAA AACTTATTCG TCTAATGGAA GAGATCATGA 
     2041 GTGAGAAGGA GAATAAAACC ATTGTTTTTG TGGAAACCAA AAGAAGATGT GATGAGCTTA 
     2101 CCAGAAAAAT GAGGAGAGAT GGGTGGCCTG CCATGGGTAT CCATGGTGAC AAGAGTCAAC 
     2161 AAGAGCGTGA CTGGGTTCTA AATGAATTCA AACATGGAAA AGCTCCTATT CTGATTGCTA 
     2221 CAGATGTGGC CTCCAGAGGG CTAGATGTGG AAGATGTGAA ATTTGTCATC AATTATGACT 
     2281 ACCCTAACTC CTCAGAGGAT TATATTCATC GAATTGGAAG AACTGCTCGC AGTACCAAAA 
     2341 CAGGCACAGC ATACACTTTC TTTACACCTA ATAACATAAA GCAAGTGAGC GACCTTATCT 
     2401 CTGTGCTTCG TGAAGCTAAT CAAGCAATTA ATCCCAAGTT GCTTCAGTTG GTCGAAGACA 
     2461 GAGGTTCAGG TCGTTCCAGG GGTAGAGGAG GCATGAAGGA TGACCGTCGG GACAGATACT 
     2521 CTGCGGGCAA AAGGGGTGGA TTTAATACCT TTAGAGACAG GGAAAATTAT GACAGAGGTT 
     2581 ACTCTAGCCT GCTTAAAAGA GATTTTGGGG CAAAAACTCA GAATGGTGTT TACAGTGCTG 
     2641 CAAATTACAC CAATGGGAGC TTTGGAAGTA ATTTTGTGTC TGCTGGTATA CAGACCAGTT 
     2701 TTAGGACTGG TAATCCAACA GGGACTTACC AGAATGGTTA TGATAGCACT CAGCAATACG 
     2761 GAAGTAATGT TCCAAATATG CACAATGGTA TGAACCAACA GGCATATGCA TATCCTGCTA 
     2821 CTGCAGCTGC ACCTATGATT GGTTATCCAA TGCCAACAGG ATATTCCCAA ACGCGTACGC 
     2881 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     2941 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3001 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3061 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3121 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3181 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3241 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3301 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3361 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3421 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3481 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3541 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3601 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3661 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3721 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     3781 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     3841 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     3901 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     3961 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4021 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4081 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4141 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4201 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4261 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4321 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4381 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4441 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4501 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4561 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4621 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4681 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4741 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     4801 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     4861 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     4921 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     4981 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5041 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5101 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5161 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5221 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5281 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5341 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5401 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5461 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5521 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5581 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5641 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5701 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     5761 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     5821 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     5881 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     5941 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6001 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6061 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6121 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6181 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6241 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6301 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6361 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6421 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6481 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6541 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6601 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6661 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6721 TTT
//