LOCUS       RC200338                8136 bp    DNA     circular  SYN 
DEFINITION  SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1
ACCESSION   NM_005072
VERSION     NM_005072.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4293..4384 
                     /label="Myc-DDK"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    4471..4490 
                     /label="Restriction Enzyme"  
     misc_feature    5275..5947 
                     /label="ColE1"  
     misc_feature    6377..7171 
                     /label="Kan/Neo"  
     misc_feature    7206..7508 
                     /label="SV40 ori"  
     misc_feature    7594..8071 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4275..4281 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4275 
                     /label="SLC12A4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCACTTC ACCGTGGTGC CAGTGGACGG GCCGAGGCGC GGCGACTATG 
     1081 ACAACCTCGA GGGGCTCAGT TGGGTGGACT ACGGGGAGCG CGCCGAGCTG GATGACTCGG 
     1141 ACGGACATGG CAACCACAGA GAGAGCAGCC CTTTTCTTTC CCCCTTGGAG GCTTCCAGAG 
     1201 GAATTGACTA CTATGACAGG AACCTGGCAC TGTTTGAGGA AGAGCTGGAC ATCCGCCCAA 
     1261 AGGTATCGTC TCTTCTGGGA AAGCTCGTCA GCTACACCAA CCTCACCCAG GGCGCCAAAG 
     1321 AGCATGAGGA GGCCGAGAGT GGGGAGGGCA CCCGCCGGAG GGCAGCCGAG GCACCCAGCA 
     1381 TGGGCACCCT CATGGGGGTG TACCTGCCCT GCCTGCAGAA TATCTTTGGG GTTATCCTCT 
     1441 TCCTGCGGCT GACCTGGATG GTGGGCACAG CAGGTGTGCT ACAGGCCCTC CTCATCGTGC 
     1501 TTATCTGCTG CTGTTGTACC CTGCTGACGG CCATCTCCAT GAGTGCCATC GCCACCAACG 
     1561 GTGTGGTTCC AGCTGGGGGC TCCTATTTCA TGATCTCTCG TTCACTGGGG CCAGAATTTG 
     1621 GAGGTGCTGT GGGCCTGTGC TTCTACCTGG GAACAACATT CGCAGCAGCC ATGTACATCC 
     1681 TGGGGGCCAT CGAGATCTTG CTGACCTACA TTGCCCCACC AGCTGCCATT TTTTACCCAT 
     1741 CGGGTGCTCA TGACACGTCG AATGCCACTT TGAACAATAT GCGTGTGTAT GGGACCATTT 
     1801 TCCTGACCTT CATGACCCTG GTGGTGTTTG TGGGGGTCAA GTATGTGAAC AAATTTGCCT 
     1861 CGCTCTTCCT GGCCTGTGTG ATCATCTCCA TCCTCTCCAT CTATGCTGGG GGCATAAAGT 
     1921 CTATATTTGA CCCTCCCGTG TTTCCGGTAT GCATGCTGGG CAACAGGACC CTGTCCCGGG 
     1981 ACCAGTTTGA CATCTGTGCC AAGACAGCTG TAGTGGACAA TGAGACAGTG GCCACCCAGC 
     2041 TATGGAGTTT CTTCTGCCAC AGCCCCAACC TTACGACCGA CTCCTGTGAC CCCTACTTCA 
     2101 TGCTCAACAA TGTGACCGAG ATCCCTGGCA TCCCCGGGGC AGCTGCTGGT GTGCTCCAGG 
     2161 AAAACCTGTG GAGCGCCTAC CTGGAGAAGG GTGACATCGT GGAGAAGCAT GGGCTGCCCT 
     2221 CCGCAGATGC CCCGAGCCTG AAGGAGAGCC TGCCTCTGTA CGTGGTCGCT GACATCGCCA 
     2281 CATCCTTCAC CGTGCTGGTC GGCATCTTCT TCCCTTCTGT AACAGGCATC ATGGCTGGCT 
     2341 CAAACCGCTC TGGGGACCTT CGTGACGCCC AGAAGTCTAT CCCTGTGGGG ACCATTCTGG 
     2401 CCATCATTAC AACTTCCCTC GTGTACTTCA GCAGTGTGGT TCTCTTTGGT GCCTGCATTG 
     2461 AGGGTGTGGT TCTCCGGGAC AAGTATGGCG ATGGTGTCAG CAGGAACTTG GTGGTGGGCA 
     2521 CACTGGCCTG GCCTTCACCC TGGGTCATCG TCATCGGCTC CTTCTTTTCA ACGTGTGGCG 
     2581 CTGGCCTCCA GAGCCTCACA GGGGCACCAC GCCTATTGCA GGCCATTGCC AAGGACAACA 
     2641 TCATCCCCTT CCTCCGGGTG TTTGGCCACG GGAAGGTGAA TGGTGAACCC ACATGGGCAC 
     2701 TCCTCCTGAC GGCACTCATC GCCGAGCTGG GCATCCTCAT CGCCTCCCTC GACATGGTGG 
     2761 CCCCCATCTT ATCCATGTTC TTTCTGATGT GCTACCTGTT CGTGAACCTC GCCTGTGCGG 
     2821 TGCAGACACT CCTGAGGACC CCCAACTGGC GGCCCCGGTT CAAGTACTAT CACTGGGCGC 
     2881 TGTCCTTCCT GGGCATGAGT CTCTGCCTGG CCCTTATGTT TGTCTCCTCC TGGTACTATG 
     2941 CCCTGGTGGC CATGCTCATC GCCGGCATGA TCTACAAATA CATCGAGTAC CAAGGGGCTG 
     3001 AGAAGGAGTG GGGTGACGGG ATCCGAGGCC TGTCCCTGAG CGCTGCCCGC TACGCGCTGT 
     3061 TGCGGCTGGA GGAGGGGCCT CCTCACACCA AGAACTGGCG GCCGCAGCTG CTGGTGCTGC 
     3121 TGAAGCTGGA CGAGGACCTC CACGTGAAGT ACCCGCGGCT CCTCACCTTC GCCTCCCAGC 
     3181 TCAAGGCTGG CAAGGGCCTG ACCATTGTTG GTTCTGTCAT CCAGGGGAGC TTCTTGGAGA 
     3241 GCTATGGCGA GGCTCAGGCC GCCGAGCAGA CCATCAAGAA CATGATGGAA ATTGAGAAGG 
     3301 TGAAGGGCTT CTGCCAGGTG GTGGTGGCCA GCAAGGTGCG GGAGGGGCTG GCCCACCTCA 
     3361 TCCAGTCCTG TGGCCTGGGA GGCATGCGGC ATAACTCCGT GGTGCTGGGC TGGCCCTACG 
     3421 GCTGGCGACA GAGCGAGGAC CCCCGTGCCT GGAAGACCTT CATTGACACC GTGCGCTGCA 
     3481 CTACGGCTGC CCACCTGGCC CTGCTCGTGC CCAAGAACAT CGCCTTCTAC CCCAGCAACC 
     3541 ACGAGCGCTA CCTGGAGGGC CACATAGACG TGTGGTGGAT CGTGCACGAT GGTGGCATGC 
     3601 TCATGCTTCT GCCCTTCCTG CTGCGCCAGC ATAAGGTCTG GAGGAAGTGC CGGATGCGCA 
     3661 TCTTCACAGT GGCCCAGATG GATGACAACA GCATCCAGAT GAAGAAGGAC CTGGCTGTCT 
     3721 TTCTGTACCA TCTGCGCCTT GAGGCCGAGG TGGAGGTGGT GGAGATGCAT AACAGTGACA 
     3781 TCTCTGCATA CACCTACGAG CGGACGCTGA TGATGGAGCA GCGGTCGCAG ATGCTGCGGC 
     3841 AGATGAGACT GACCAAGACT GAGCGGGAGC GAGAAGCCCA GCTGGTCAAG GATCGGCACT 
     3901 CGGCCCTGAG GCTGGAGAGC CTGTACTCGG ACGAGGAAGA TGAGTCTGCA GTGGGGGCTG 
     3961 ACAAGATCCA GATGACGTGG ACCAGGGACA AGTACATGAC TGAGACCTGG GACCCCAGCC 
     4021 ATGCCCCTGA CAATTTCCGG GAGCTGGTGC ACATTAAGCC GGACCAATCC AATGTGCGGC 
     4081 GCATGCACAC TGCTGTGAAG CTCAATGAAG TCATTGTCAC GCGCTCCCAC GACGCCCGCC 
     4141 TGGTTCTCCT AAACATGCCT GGCCCACCCA GGAACAGTGA GGGCGACGAG AACTACATGG 
     4201 AGTTCCTCGA GGTGCTGACC GAGGGCCTTG AGCGGGTGCT GTTGGTGCGC GGTGGTGGCC 
     4261 GTGAAGTCAT CACCATCTAC TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//