LOCUS       RC200324L4                8324 bp    DNA     circular  SYN 
DEFINITION  ASF1A (Myc-DDK-tagged)-Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A)
ACCESSION   NM_014034
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6615..6795 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7523..8169 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    5490..5510 
                     /label="LR50"  
     misc_feature    3953..4681 
                     /label="mGFP"  
     misc_feature    4688..4753 
                     /label="P2A"  
     misc_feature    6843..7462 
                     /label="pBR322 Ori"  
     misc_feature    4754..5353 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    3932..3938 
                     /label="Restriction Enzyme"  
     misc_feature    3320..3932 
                     /label="ASF1A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAAAGGTT CAGGTGAACA ATGTAGTGGT 
     3361 GCTGGATAAC CCTTCTCCTT TCTACAACCC GTTCCAGTTC GAGATCACCT TCGAGTGCAT 
     3421 CGAGGACCTG TCTGAAGACT TGGAATGGAA AATTATCTAT GTGGGCTCTG CAGAAAGTGA 
     3481 AGAATACGAT CAAGTTTTAG ACTCTGTTTT AGTGGGTCCT GTTCCCGCAG GAAGGCATAT 
     3541 GTTTGTATTT CAGGCTGATG CACCTAATCC AGGACTCATT CCAGATGCAG ATGCAGTAGG 
     3601 CGTAACTGTT GTGCTAATTA CTTGTACCTA TCGAGGACAA GAATTTATTA GAGTTGGCTA 
     3661 TTATGTAAAT AATGAATATA CTGAGACAGA ATTAAGGGAA AATCCACCAG TAAAACCAGA 
     3721 CTTTTCTAAG CTTCAAAGGA ATATTTTGGC ATCTAATCCC AGGGTCACAA GATTCCACAT 
     3781 TAATTGGGAA GATAACACAG AAAAACTGGA AGATGCAGAG AGCAGTAATC CAAATCTACA 
     3841 GTCACTTCTT TCAACAGATG CATTACCTTC AGCATCAAAG GGATGGTCCA CATCAGAAAA 
     3901 CTCACTAAAT GTCATGTTAG AATCCCACAT GGACTGCATG ACGCGTACGC GGCCGCTCGA 
     3961 GATGAGCGGG GGCGAGGAGC TGTTCGCCGG CATCGTGCCC GTGCTGATCG AGCTGGACGG 
     4021 CGACGTGCAC GGCCACAAGT TCAGCGTGCG CGGCGAGGGC GAGGGCGACG CCGACTACGG 
     4081 CAAGCTGGAG ATCAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT 
     4141 GGTGACCACC CTCTGCTACG GCATCCAGTG CTTCGCCCGC TACCCCGAGC ACATGAAGAT 
     4201 GAACGACTTC TTCAAGAGCG CCATGCCCGA GGGCTACATC CAGGAGCGCA CCATCCAGTT 
     4261 CCAGGACGAC GGCAAGTACA AGACCCGCGG CGAGGTGAAG TTCGAGGGCG ACACCCTGGT 
     4321 GAACCGCATC GAGCTGAAGG GCAAGGACTT CAAGGAGGAC GGCAACATCC TGGGCCACAA 
     4381 GCTGGAGTAC AGCTTCAACA GCCACAACGT GTACATCCGC CCCGACAAGG CCAACAACGG 
     4441 CCTGGAGGCT AACTTCAAGA CCCGCCACAA CATCGAGGGC GGCGGCGTGC AGCTGGCCGA 
     4501 CCACTACCAG ACCAACGTGC CCCTGGGCGA CGGCCCCGTG CTGATCCCCA TCAACCACTA 
     4561 CCTGAGCACT CAGACCAAGA TCAGCAAGGA CCGCAACGAG GCCCGCGACC ACATGGTGCT 
     4621 CCTGGAGTCC TTCAGCGCCT GCTGCCACAC CCACGGCATG GACGAGCTGT ACAGGTCCGG 
     4681 ACTCAGAGTT TGGGTAGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA 
     4741 CGTGGAGGAG AACCCTGGAC CTATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG 
     4801 CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC 
     4861 GCGCCACACC GTCGATCCGG ATCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT 
     4921 CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT 
     4981 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC 
     5041 GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT 
     5101 GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA 
     5161 CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG 
     5221 CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG 
     5281 GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT 
     5341 GACCCGCAAG CCCGGTGCCT GATGTACACC CAAACGGCCG GCCGCGGTCT GTACAAGTAG 
     5401 GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT 
     5461 AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG 
     5521 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 
     5581 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 
     5641 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 
     5701 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 
     5761 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 
     5821 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 
     5881 ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA 
     5941 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 
     6001 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC 
     6061 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA 
     6121 GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT 
     6181 GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT 
     6241 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 
     6301 GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC 
     6361 CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT 
     6421 CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA 
     6481 GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC 
     6541 CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC 
     6601 CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG 
     6661 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT 
     6721 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT 
     6781 TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 
     6841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 
     6901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 
     6961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 
     7021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 
     7081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 
     7141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 
     7201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 
     7261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 
     7321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 
     7381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 
     7441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 
     7501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT 
     7561 ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA 
     7621 CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA 
     7681 AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA 
     7741 CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA 
     7801 GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT 
     7861 CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC 
     7921 AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG 
     7981 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 
     8041 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 
     8101 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 
     8161 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8221 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8281 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//