LOCUS RC200311 6672 bp DNA circular SYN DEFINITION KARS (Myc-DDK-tagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2 ACCESSION NM_005548 VERSION NM_005548.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2829..2920 /label="Myc-DDK" misc_feature 2950..3535 /label="PolyA signal" misc_feature 3007..3026 /label="Restriction Enzyme" misc_feature 3811..4483 /label="ColE1" misc_feature 4913..5707 /label="Kan/Neo" misc_feature 5742..6044 /label="SV40 ori" misc_feature 6130..6607 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2811..2817 /label="Restriction Enzyme" misc_feature 1020..2811 /label="KARS" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGGCCGTG CAGGCGGCCG AGGTGAAAGT GGATGGCAGC GAGCCGAAAC 1081 TGAGCAAGAA TGAGCTGAAG AGACGCCTGA AAGCTGAGAA GAAAGTAGCA GAGAAGGAGG 1141 CCAAACAGAA GGAGCTCAGT GAGAAACAGC TAAGCCAAGC CACTGCTGCT GCCACCAACC 1201 ACACCACTGA TAATGGTGTG GGTCCTGAGG AAGAGAGCGT GGACCCAAAT CAATACTACA 1261 AAATCCGCAG TCAAGCAATT CATCAGCTGA AGGTCAATGG GGAAGACCCA TACCCACACA 1321 AGTTCCATGT AGACATCTCA CTCACTGACT TCATCCAAAA ATATAGTCAC CTGCAGCCTG 1381 GGGATCACCT GACTGACATC ACCTTAAAGG TGGCAGGTAG GATCCATGCC AAAAGAGCTT 1441 CTGGGGGAAA GCTCATCTTC TATGATCTTC GAGGAGAGGG GGTGAAGTTG CAAGTCATGG 1501 CCAATTCCAG AAATTATAAA TCAGAAGAAG AATTTATTCA TATTAATAAC AAACTGCGTC 1561 GGGGAGACAT AATTGGAGTT CAGGGGAATC CTGGTAAAAC CAAGAAGGGT GAGCTGAGCA 1621 TCATTCCGTA TGAGATCACA CTGCTGTCTC CCTGTTTGCA TATGTTACCT CATCTTCACT 1681 TTGGCCTCAA AGACAAGGAA ACAAGGTATC GCCAGAGATA CTTGGACTTG ATCCTGAATG 1741 ACTTTGTGAG GCAGAAATTT ATCATCCGCT CTAAGATCAT CACATATATA AGAAGTTTCT 1801 TAGATGAGCT GGGATTCCTA GAGATTGAAA CTCCCATGAT GAACATCATC CCAGGGGGAG 1861 CCGTGGCCAA GCCTTTCATC ACTTATCACA ACGAGCTGGA CATGAACTTA TATATGAGAA 1921 TTGCTCCAGA ACTCTATCAT AAGATGCTTG TGGTTGGTGG CATCGACCGG GTTTATGAAA 1981 TTGGACGCCA GTTCCGGAAT GAGGGGATTG ATTTGACGCA CAATCCTGAG TTCACCACCT 2041 GTGAGTTCTA CATGGCCTAT GCAGACTATC ACGATCTCAT GGAAATCACG GAGAAGATGG 2101 TTTCAGGGAT GGTGAAGCAT ATTACAGGCA GTTACAAGGT CACCTACCAC CCAGATGGCC 2161 CAGAGGGCCA AGCCTACGAT GTTGACTTCA CCCCACCCTT CCGGCGAATC AACATGGTAG 2221 AAGAGCTTGA GAAAGCCCTG GGGATGAAGC TGCCAGAAAC GAACCTCTTT GAAACTGAAG 2281 AAACTCGCAA AATTCTTGAT GATATCTGTG TGGCAAAAGC TGTTGAATGC CCTCCACCTC 2341 GGACCACAGC CAGGCTCCTT GACAAGCTTG TTGGGGAGTT CCTGGAAGTG ACTTGCATCA 2401 ATCCTACATT CATCTGTGAT CACCCACAGA TAATGAGCCC TTTGGCTAAA TGGCACCGCT 2461 CTAAAGAGGG TCTGACTGAG CGCTTTGAGC TGTTTGTCAT GAAGAAAGAG ATATGCAATG 2521 CGTATACTGA GCTGAATGAT CCCATGCGGC AGCGGCAGCT TTTTGAAGAA CAGGCCAAGG 2581 CCAAGGCTGC AGGTGATGAT GAGGCCATGT TCATAGATGA AAACTTCTGT ACTGCCCTGG 2641 AATATGGGCT GCCCCCCACA GCTGGCTGGG GCATGGGCAT TGATCGAGTC GCCATGTTTC 2701 TCACGGACTC CAACAACATC AAGGAAGTAC TTCTGTTTCC TGCCATGAAA CCCGAAGACA 2761 AGAAGGAGAA TGTAGCAACC ACTGATACAC TGGAAAGCAC AACAGTTGGC ACTTCTGTCA 2821 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 2881 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 2941 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 3001 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 3061 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 3121 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 3181 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 3241 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 3301 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 3361 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 3421 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 3481 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 3541 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 3601 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 3661 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 3721 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 3781 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3841 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 3901 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 3961 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 4021 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 4081 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 4141 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 4201 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 4261 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 4321 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 4381 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 4441 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 4501 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 4561 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 4621 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 4681 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 4741 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 4801 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 4861 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 4921 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 4981 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 5041 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 5101 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 5161 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 5221 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 5281 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 5341 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 5401 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 5461 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 5521 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 5581 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 5641 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 5701 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 5761 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 5821 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 5881 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 5941 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 6001 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 6061 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 6121 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 6181 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 6241 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 6301 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 6361 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 6421 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 6481 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 6541 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 6601 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 6661 AACGCGAATT TT //