LOCUS       RC200151                7713 bp    DNA     circular  SYN 
DEFINITION  UNC45A (Myc-DDK-tagged)-Human unc-45 homolog A (C. elegans) (UNC45A), transcript variant 2
ACCESSION   NM_018671
VERSION     NM_018671.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="UNC45A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGTGAGT GGTCCAGGGA CCCCCGAGCC CCGGCCGGCC ACCCCCGGGG 
     1081 CCAGCTCAGT GGAGCAGCTG CGGAAGGAGG GCAATGAGCT GTTCAAATGT GGAGACTACG 
     1141 GGGGCGCCCT GGCGGCCTAC ACTCAGGCCC TGGGTCTGGA CGCGACGCCC CAGGACCAGG 
     1201 CCGTTCTGCA CCGGAACCGG GCCGCCTGCC ACCTCAAGCT GGAAGATTAC GACAAAGCAG 
     1261 AAACAGAGGC ATCCAAAGCC ATTGAAAAGG ATGGTGGGGA TGTCAAAGCA CTCTACCGGC 
     1321 GGAGCCAAGC CCTAGAGAAG CTGGGCCGCC TGGACCAGGC TGTCCTTGAC CTGCAGAGAT 
     1381 GTGTGAGCTT GGAGCCCAAG AACAAAGTTT TCCAGGAGGC CTTGCGGAAC ATCGGGGGCC 
     1441 AGATTCAGGA GAAGGTGCGA TACATGTCCT CGACGGATGC CAAAGTGGAA CAGATGTTTC 
     1501 AGATACTGTT GGACCCAGAA GAGAAGGGCA CTGAGAAAAA GCAAAAGGCT TCTCAGAACC 
     1561 TGGTGGTGCT GGCCAGGGAG GATGCTGGAG CGGAGAAGAT CTTCCGGAGT AATGGGGTTC 
     1621 AGCTCTTGCA ACGTTTACTG GACATGGGAG AGACTGACCT CATGCTGGCG GCTCTGCGTA 
     1681 CGCTGGTTGG CATTTGCTCT GAGCATCAGT CACGGACAGT GGCAACCCTG AGCATACTGG 
     1741 GAACTCGGCG AGTAGTCTCC ATCCTGGGCG TGGAAAGCCA GGCTGTGTCC CTGGCTGCCT 
     1801 GCCACCTGCT GCAGGTTATG TTTGATGCCC TCAAGGAAGG TGTCAAAAAA GGCTTCCGAG 
     1861 GCAAAGAAGG TGCCATCATT GTGGATCCTG CCCGGGAGCT GAAGGTCCTC ATCAGTAACC 
     1921 TCTTAGATCT GCTGACAGAG GTGGGGGTCT CTGGCCAAGG CCGAGACAAT GCCCTGACCC 
     1981 TCCTGATTAA AGCGGTGCCC CGGAAGTCTC TCAAGGACCC CAACAACAGC CTCACCCTCT 
     2041 GGGTCATCGA CCAAGGTCTG AAAAAGATTT TGGAAGTGGG GGGCTCTCTA CAGGACCCTC 
     2101 CTGGGGAGCT CGCAGTGACC GCAAACAGCC GCATGAGCGC CTCTATTCTC CTCAGCAAGC 
     2161 TCTTTGATGA CCTCAAGTGT GATGCGGAGA GGGAGAATTT CCACAGACTT TGTGAAAACT 
     2221 ACATCAAGAG CTGGTTTGAG GGCCAAGGGC TGGCCGGGAA GCTACGGGCC ATCCAGACGG 
     2281 TGTCCTGCCT CCTGCAGGGC CCATGTGACG CTGGCAACCG GGCCTTGGAG CTGAGCGGTG 
     2341 TCATGGAGAG TGTGATTGCT CTGTGTGCCT CTGAGCAGGA GGAGGAGCAG CTGGTGGCCG 
     2401 TGGAGGCTCT GATCCATGCA GCCGGCAAGG CTAAGCGGGC CTCATTCATC ACTGCCAATG 
     2461 GTGTCTCGCT GCTGAAGGAC CTATATAAGT GCAGCGAGAA GGACAGCATC CGCATCCGGG 
     2521 CGCTAGTGGG ACTCTGTAAG CTCGGTTCGG CTGGAGGGAC TGACTTCAGC ATGAAGCAGT 
     2581 TTGCTGAAGG CTCCACTCTC AAACTGGCTA AGCAGTGTCG AAAGTGGCTG TGCAATGACC 
     2641 AGATCGACGC AGGCACTCGG CGCTGGGCAG TGGAGGGCCT GGCTTACCTG ACCTTTGATG 
     2701 CCGACGTGAA GGAAGAGTTT GTGGAGGATG CGGCTGCTCT GAAAGCTCTG TTCCAGCTCA 
     2761 GCAGGTTGGA GGAGAGGTCA GTGCTCTTTG CGGTGGCCTC AGCGCTGGTG AACTGCACCA 
     2821 ACAGCTATGA CTACGAGGAG CCCGACCCCA AGATGGTGGA GCTGGCCAAG TATGCCAAGC 
     2881 AGCATGTGCC CGAGCAGCAC CCCAAGGACA AGCCAAGCTT CGTGCGGGCT CGGGTGAAGA 
     2941 AGCTGCTGGC AGCGGGTGTG GTGTCGGCCA TGGTGTGCAT GGTGAAGACG GAGAGCCCTG 
     3001 TGCTGACCAG TTCCTGCAGA GAGCTGCTCT CCAGGGTCTT CTTGGCTTTA GTGGAAGAGG 
     3061 TAGAGGACCG AGGCACTGTG GTTGCCCAGG GAGGCGGCAG GGCGCTGATC CCGCTGGCCC 
     3121 TGGAAGGCAC GGACGTGGGG CAGACAAAGG CAGCCCAGGC CCTTGCCAAG CTCACCATCA 
     3181 CCTCCAACCC GGAGATGACC TTCCCTGGCG AGCGGATCTA TGAGGTGGTC CGGCCCCTCG 
     3241 TCTCCCTGTT GCACCTCAAC TGCTCAGGCC TGCAGAACTT CGAGGCGCTC ATGGCCCTAA 
     3301 CAAACCTGGC TGGGATCAGC GAGAGGCTCC GGCAGAAGAT CCTGAAGGAG AAGGCTGTGC 
     3361 CCATGATAGA AGGCTACATG TTTGAGGAGC ATGAGATGAT CCGCCGGGCA GCCACGGAGT 
     3421 GCATGTGTAA CTTGGCCATG AGCAAGGAGG TGCAGGACCT CTTCGAAGCC CAGGGCAATG 
     3481 ACCGACTGAA GCTGCTGGTG CTGTACAGTG GAGAGGATGA TGAGCTGCTA CAGCGGGCAG 
     3541 CTGCCGGGGG CTTGGCCATG CTTACCTCCA TGCGGCCCAC GCTCTGCAGC CGCATTCCCC 
     3601 AAGTGACCAC ACACTGGCTG GAGATCCTGC AGGCCCTGCT TCTGAGCTCC AACCAGGAGC 
     3661 TGCAGCACCG GGGTGCTGTG GTGGTGCTGA ACATGGTGGA GGCCTCGAGG GAGATTGCCA 
     3721 GCACCCTGAT GGAGAGTGAG ATGATGGAGA TCTTGTCAGT GCTAGCTAAG GGTGACCACA 
     3781 GCCCTGTCAC AAGGGCTGCT GCAGCCTGCC TGGACAAAGC AGTGGAATAT GGGCTTATCC 
     3841 AACCCAACCA AGATGGAGAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//