LOCUS       RC200093                7452 bp    DNA     circular  SYN 
DEFINITION  TTK (Myc-DDK-tagged)-Human TTK protein kinase (TTK), transcript variant 1
ACCESSION   NM_003318
VERSION     NM_003318.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3609..3700 
                     /label="Myc-DDK"  
     misc_feature    3730..4315 
                     /label="PolyA signal"  
     misc_feature    3787..3806 
                     /label="Restriction Enzyme"  
     misc_feature    4591..5263 
                     /label="ColE1"  
     misc_feature    5693..6487 
                     /label="Kan/Neo"  
     misc_feature    6522..6824 
                     /label="SV40 ori"  
     misc_feature    6910..7387 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3591..3597 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3591 
                     /label="TTK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATCCGAG GATTTAAGTG GCAGAGAATT GACAATTGAT TCCATAATGA 
     1081 ACAAAGTGAG AGACATTAAA AATAAGTTTA AAAATGAAGA CCTTACTGAT GAACTAAGCT 
     1141 TGAATAAAAT TTCTGCTGAT ACTACAGATA ACTCGGGAAC TGTTAACCAA ATTATGATGA 
     1201 TGGCAAACAA CCCAGAGGAC TGGTTGAGTT TGTTGCTCAA ACTAGAGAAA AACAGTGTTC 
     1261 CGCTAAGTGA TGCTCTTTTA AATAAATTGA TTGGTCGTTA CAGTCAAGCA ATTGAAGCGC 
     1321 TTCCCCCAGA TAAATATGGC CAAAATGAGA GTTTTGCTAG AATTCAAGTG AGATTTGCTG 
     1381 AATTAAAAGC TATTCAAGAG CCAGATGATG CACGTGACTA CTTTCAAATG GCCAGAGCAA 
     1441 ACTGCAAGAA ATTTGCTTTT GTTCATATAT CTTTTGCACA ATTTGAACTG TCACAAGGTA 
     1501 ATGTCAAAAA AAGTAAACAA CTTCTTCAAA AAGCTGTAGA ACGTGGAGCA GTACCACTAG 
     1561 AAATGCTGGA AATTGCCCTG CGGAATTTAA ACCTCCAAAA AAAGCAGCTG CTTTCAGAGG 
     1621 AGGAAAAGAA GAATTTATCA GCATCTACGG TATTAACTGC CCAAGAATCA TTTTCCGGTT 
     1681 CACTTGGGCA TTTACAGAAT AGGAACAACA GTTGTGATTC CAGAGGACAG ACTACTAAAG 
     1741 CCAGGTTTTT ATATGGAGAG AACATGCCAC CACAAGATGC AGAAATAGGT TACCGGAATT 
     1801 CATTGAGACA AACTAACAAA ACTAAACAGT CATGCCCATT TGGAAGAGTC CCAGTTAACC 
     1861 TTCTAAATAG CCCAGATTGT GATGTGAAGA CAGATGATTC AGTTGTACCT TGTTTTATGA 
     1921 AAAGACAAAC CTCTAGATCA GAATGCCGAG ATTTGGTTGT GCCTGGATCT AAACCAAGTG 
     1981 GAAATGATTC CTGTGAATTA AGAAATTTAA AGTCTGTTCA AAATAGTCAT TTCAAGGAAC 
     2041 CTCTGGTGTC AGATGAAAAG AGTTCTGAAC TTATTATTAC TGATTCAATA ACCCTGAAGA 
     2101 ATAAAACGGA ATCAAGTCTT CTAGCTAAAT TAGAAGAAAC TAAAGAGTAT CAAGAACCAG 
     2161 AGGTTCCAGA GAGTAACCAG AAACAGTGGC AATCTAAGAG AAAGTCAGAG TGTATTAACC 
     2221 AGAATCCTGC TGCATCTTCA AATCACTGGC AGATTCCGGA GTTAGCCCGA AAAGTTAATA 
     2281 CAGAGCAGAA ACATACCACT TTTGAGCAAC CTGTCTTTTC AGTTTCAAAA CAGTCACCAC 
     2341 CAATATCAAC ATCTAAATGG TTTGACCCAA AATCTATTTG TAAGACACCA AGCAGCAATA 
     2401 CCTTGGATGA TTACATGAGC TGTTTTAGAA CTCCAGTTGT AAAGAATGAC TTTCCACCTG 
     2461 CTTGTCAGTT GTCAACACCT TATGGCCAAC CTGCCTGTTT CCAGCAGCAA CAGCATCAAA 
     2521 TACTTGCCAC TCCACTTCAA AATTTACAGG TTTTAGCATC TTCTTCAGCA AATGAATGCA 
     2581 TTTCGGTTAA AGGAAGAATT TATTCCATTT TAAAGCAGAT AGGAAGTGGA GGTTCAAGCA 
     2641 AGGTATTTCA GGTGTTAAAT GAAAAGAAAC AGATATATGC TATAAAATAT GTGAACTTAG 
     2701 AAGAAGCAGA TAACCAAACT CTTGATAGTT ACCGGAACGA AATAGCTTAT TTGAATAAAC 
     2761 TACAACAACA CAGTGATAAG ATCATCCGAC TTTATGATTA TGAAATCACG GACCAGTACA 
     2821 TCTACATGGT AATGGAGTGT GGAAATATTG ATCTTAATAG TTGGCTTAAA AAGAAAAAAT 
     2881 CCATTGATCC ATGGGAACGC AAGAGTTACT GGAAAAATAT GTTAGAGGCA GTTCACACAA 
     2941 TCCATCAACA TGGCATTGTT CACAGTGATC TTAAACCAGC TAACTTTCTG ATAGTTGATG 
     3001 GAATGCTAAA GCTAATTGAT TTTGGGATTG CAAACCAAAT GCAACCAGAT ACAACAAGTG 
     3061 TTGTTAAAGA TTCTCAGGTT GGCACAGTTA ATTATATGCC ACCAGAAGCA ATCAAAGATA 
     3121 TGTCTTCCTC CAGAGAGAAT GGGAAATCTA AGTCAAAGAT AAGCCCCAAA AGTGATGTTT 
     3181 GGTCCTTAGG ATGTATTTTG TACTATATGA CTTACGGGAA AACACCATTT CAGCAGATAA 
     3241 TTAATCAGAT TTCTAAATTA CATGCCATAA TTGATCCTAA TCATGAAATT GAATTTCCCG 
     3301 ATATTCCAGA GAAAGATCTT CAAGATGTGT TAAAGTGTTG TTTAAAAAGG GACCCAAAAC 
     3361 AGAGGATATC CATTCCTGAG CTCCTGGCTC ATCCCTATGT TCAAATTCAA ACTCATCCAG 
     3421 TTAACCAAAT GGCCAAGGGA ACCACTGAAG AAATGAAATA TGTTCTGGGC CAACTTGTTG 
     3481 GTCTGAATTC TCCTAACTCC ATTTTGAAAG CTGCTAAAAC TTTATATGAA CACTATAGTG 
     3541 GTGGTGAAAG TCATAATTCT TCATCCTCCA AGACTTTTGA AAAAAAAAGG GGAAAAAAAA 
     3601 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3661 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3721 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3781 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3841 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3901 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3961 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4021 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4081 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4141 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4201 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4261 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4321 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4381 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4441 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4501 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4561 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4621 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4681 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4741 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4801 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4861 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4921 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4981 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5041 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5101 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5161 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5221 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5281 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5341 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5401 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5461 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5521 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5581 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5641 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5701 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5761 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5821 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5881 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5941 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6001 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6061 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6121 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6181 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6241 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6301 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6361 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6421 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6481 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6541 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6601 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6661 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6721 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6901 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6961 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7021 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7081 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7141 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7201 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7261 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7321 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7381 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7441 AACGCGAATT TT
//