LOCUS       MR231836                9507 bp    DNA     circular  SYN 
DEFINITION  Slit2 (myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2), transcript variant 1
ACCESSION   NM_001291227
VERSION     NM_001291227.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5664..5755 
                     /label="Myc-DDK"  
     misc_feature    5785..6370 
                     /label="PolyA signal"  
     misc_feature    5842..5861 
                     /label="Restriction Enzyme"  
     misc_feature    6646..7318 
                     /label="ColE1"  
     misc_feature    7748..8542 
                     /label="Kan/Neo"  
     misc_feature    8577..8879 
                     /label="SV40 ori"  
     misc_feature    8965..9442 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5646..5652 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5646 
                     /label="Slit2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCATT GGCTGGCAGA CACTGTCCCT ATCGCTGGGG TTAGTGTTGT 
     1081 CGATCTTGAA CAAGGTGGCG CCGCAGGCGT GCCCGGCCCA GTGCTCCTGT TCAGGCAGCA 
     1141 CGGTGGACTG TCATGGGCTG GCACTGCGCA GTGTGCCCAG GAATATCCCC CGCAACACCG 
     1201 AGAGACTGGA TTTGAATGGA AATAACATCA CGAGGATCAC GAAGATAGAT TTTGCTGGTC 
     1261 TCAGGCACCT CAGAGTTCTT CAGCTCATGG AGAACAGAAT CAGCACCATC GAGAGGGGAG 
     1321 CATTCCAGGA TCTTAAGGAG CTGGAAAGAC TGCGTTTAAA CAGAAATAAC CTTCAGTTGT 
     1381 TTCCTGAGCT GCTGTTTCTC GGGACTGCGA AGCTCTACCG GCTTGATCTC AGTGAAAATC 
     1441 AAATTCAAGC AATTCCAAGG AAGGCTTTCC GTGGGGCAGT TGACATTAAA AACCTGCAAC 
     1501 TGGATTACAA CCAGATCAGC TGCATTGAAG ATGGGGCGTT CAGAGCTCTA CGAGATCTGG 
     1561 AAGTGCTCAC TCTGAACAAT AACAATATTA CTAGACTTTC AGTGGCAAGT TTCAACCATA 
     1621 TGCCTAAACT TAGGACATTT CGACTCCACT CGAACAACTT GTACTGCGAC TGCCACCTAG 
     1681 CCTGGCTCTC AGACTGGCTT CGCCAAAGGC CACGGGTGGG CTTGTACACT CAGTGTATGG 
     1741 GCCCATCCCA CCTGAGGGGC CACAATGTAG CAGAGGTTCA AAAACGAGAG TTTGTCTGCA 
     1801 GTGATGAGGA AGAAGGTCAC CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC 
     1861 CCGCTGCTTG TACCTGTAGC AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA 
     1921 TCCCCACAAA TCTGCCTGAG ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG 
     1981 TCATCCCTCC AGGAGCCTTC TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA 
     2041 ACCAGATCTC TGAACTTGCA CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG 
     2101 TCCTGTATGG AAATAAAATC ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT 
     2161 TGCAGCTACT ATTATTGAAT GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG 
     2221 ACCTGCACAA CTTGAACCTT CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG 
     2281 GCACCTTCTC AGCCCTCAGA GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT 
     2341 GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG 
     2401 GTGCCCGTTG CACCAGCCCC CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA 
     2461 AGAAATTCCG TTGTTCAGCT AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT 
     2521 CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG 
     2581 GGACCACAGT AGACTGCTCC AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT 
     2641 ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT 
     2701 TTAAGAAACT TCCTCAGTTA CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG 
     2761 AGGAGGGTGC ATTTGAAGGC GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT 
     2821 TGGAAAATGT TCAGCATAAG ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA 
     2881 GAAGTAATCG AATAAGCTGT GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC 
     2941 TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC 
     3001 ATTCATTATC CACTCTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT 
     3061 GGCTGGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC 
     3121 CCTACTTCCT GAAGGAAATC CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG 
     3181 ATGGAAATGA TGACAATAGT TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT 
     3241 TGGATACAGT GGTACGATGT AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA 
     3301 AAGATGTCAC AGAGCTGTAT CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT 
     3361 CTAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA 
     3421 ATCAAAGCTT CAGCAACATG ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA 
     3481 GATGTATCCC TCCACGAACC TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG 
     3541 GAAATGACAT TTCTGTTGTG CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT 
     3601 TAGCGATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG 
     3661 TGAAGTCGGA ATATAAGGAA CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG 
     3721 ATAAATTATT ACTCACTACT CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA 
     3781 CTATTCAAGC CAAGTGTAAT CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA 
     3841 ACAATGACCC CGTTGATTTT TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT 
     3901 GTGATGTCCC CATTCATGCT TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT 
     3961 TAAAGGAAGG AGAGAATGCT GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA 
     4021 ACTGTGAAGT CAATATTGAT GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG 
     4081 TTGATGGAAT TAACAACTAC ACATGTCTTT GCCCACCGGA ATACACAGCT GCTAATCTGA 
     4141 ATGAGGTGGA AAAAGGTGAA CTGTGTGAGG AAAAGCTGGA CTTCTGTGCA CAAGACTTGA 
     4201 ATCCCTGCCA GCATGACTCC AAGTGCATCC TGACTCCAAA GGGATTCAAG TGTGACTGCA 
     4261 CTCCAGGATA CATTGGTGAG CACTGTGACA TTGACTTTGA TGACTGCCAA GATAACAAGT 
     4321 GTAAAAACGG TGCTCACTGC ACAGATGCCG TGAACGGATA CACGTGCGTC TGTCCTGAAG 
     4381 GCTACAGTGG CTTGTTCTGT GAGTTTTCTC CACCCATGGT CCTCCCTCGC ACCAGCCCCT 
     4441 GTGATAATTT TGATTGCCAG AATGGAGCCC AGTGTATCAT CAGGATAAAT GAACCAATAT 
     4501 GCCAGTGTTT GCCTGGCTAC CTGGGAGAGA AGTGTGAGAA ATTGGTCAGT GTGAATTTTG 
     4561 TAAACAAAGA GTCCTATCTT CAGATTCCTT CAGCCAAGGT TCGGCCTCAG ACAAACATCA 
     4621 CACTTCAGAT TGCCACAGAT GAAGACAGCG GCATCCTCTT GTATAAAGGT GACAAAGACC 
     4681 ACATTGCCGT GGAACTCTAT AGAGGGCGAG TTCGAGCCAG CTATGACACC GGCTCTCATC 
     4741 CGGCTTCTGC CATTTACAGT GTGGAGACAA TCAATGATGG AAACTTCCAC ATTGTGGAGC 
     4801 TACTGACCCT GGATTCCAGT CTTTCCCTCT CTGTGGATGG AGGAAGCCCT AAAGTCATCA 
     4861 CCAATTTGTC AAAACAATCT ACTCTGAATT TCGACTCTCC ACTCTATGTA GGAGGCATGC 
     4921 CTGGGAAAAA TAACGTGGCA TCCCTGCGCC AGGCCCCTGG GCAAAATGGC ACCAGCTTCC 
     4981 ATGGCTGTAT CCGGAACCTT TACATTAACA GTGAGCTGCA GGACTTCCGG AAAATGCCTA 
     5041 TGCAAACCGG AATTCTGCCT GGCTGTGAAC CATGCCACAA GAAAGTATGT GCCCATGGCA 
     5101 TGTGCCAGCC CAGCAGCCAA TCAGGCTTCA CCTGTGAATG TGAGGAAGGG TGGATGGGGC 
     5161 CCCTCTGTGA CCAGAGAACC AATGATCCCT GCCTCGGAAA CAAATGTGTG CATGGGACCT 
     5221 GCCTGCCCAT CAATGCCTTC TCCTATAGTT GCAAGTGCCT GGAGGGCCAT GGCGGTGTCC 
     5281 TCTGTGATGA AGAAGAAGAT CTCTTTAACC CCTGCCAGAT GATCAAGTGC AAGCATGGGA 
     5341 AGTGCAGGCT TTCTGGAGTG GGCCAGCCCT ATTGTGAATG CAACAGTGGA TTCACCGGGG 
     5401 ACAGCTGTGA TAGAGAAATT TCTTGTCGAG GGGAACGGAT AAGGGACTAT TACCAGAAGC 
     5461 AGCAGGGTTA CGCTGCCTGT CAAACAACTA AGAAAGTATC TCGCTTGGAA TGCAGAGGCG 
     5521 GGTGCGCTGG AGGCCAGTGC TGTGGACCTC TGAGAAGCAA GAGGCGGAAA TACTCTTTCG 
     5581 AATGCACAGA TGGCTCCTCA TTTGTGGACG AGGTTGAGAA AGTGGTGAAG TGCGGCTGCG 
     5641 CGAGATGTGC CTCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5701 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5761 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5821 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5881 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5941 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     6001 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     6061 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     6121 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6181 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6241 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6301 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6361 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6421 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6481 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6541 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6601 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6661 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6721 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6781 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6841 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6901 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6961 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     7021 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     7081 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     7141 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7201 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7261 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7321 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7381 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7441 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7501 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7561 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7621 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7681 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7741 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7801 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7861 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7921 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7981 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     8041 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     8101 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     8161 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8221 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8281 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8341 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8401 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8461 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8521 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8581 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8641 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8701 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8761 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8821 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8881 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8941 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9001 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     9061 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     9121 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9181 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9241 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9301 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9361 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9421 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9481 ATTTAACAAA AATTTAACGC GAATTTT
//