LOCUS       MR231823                9393 bp    DNA     circular  SYN 
DEFINITION  Cux1 (myc-DDK-tagged) - Mouse cut-like homeobox 1 (Cux1), transcript variant 4
ACCESSION   NM_001291234
VERSION     NM_001291234.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5550..5641 
                     /label="Myc-DDK"  
     misc_feature    5671..6256 
                     /label="PolyA signal"  
     misc_feature    5728..5747 
                     /label="Restriction Enzyme"  
     misc_feature    6532..7204 
                     /label="ColE1"  
     misc_feature    7634..8428 
                     /label="Kan/Neo"  
     misc_feature    8463..8765 
                     /label="SV40 ori"  
     misc_feature    8851..9328 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5532..5538 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5532 
                     /label="Cux1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCAAT GTGGGATCGA TGTTTCAATA TTGGAAGCGC TTTGATTTAC 
     1081 AGCAGCTGCA GAGAGAACTT GATGCCACCG CAACAGTATT GGCAAACAGG CAAGATGAGA 
     1141 GCGAACAGTC CAGAAAGCGG CTCATTGAGC AGAGCCGAGA ATTCAAGAAG AACACTCCAG 
     1201 AGGATTTACG CAAGCAGGTA GCACCACTGC TAAAGAGCTT CCAAGGGGAG ATTGATGCAC 
     1261 TGAGTAAAAG AAGCAAAGAA GCAGAGGCAG CCTTCTTGAC TGTGTACAAG AGACTAATTG 
     1321 ATGTTCCAGA TCCGGTACCA GCCCTGGACG TCGGGCAACA GCTGGAAATA AAAGTGCAGC 
     1381 GTCTACACGA CATTGAAACA GAGAACCAGA AACTTAGGGA AACACTAGAA GAGTACAACA 
     1441 AGGAGTTTGC TGAAGTGAAA AATCAAGAGG TTACGATAAA AGCACTTAAG GAGAAAATCC 
     1501 GAGAATACGA GCAGACCCTG AAGAGTCAGG CCGAGACCAT TGCTCTGGAG AAAGAGCAGA 
     1561 AGCTACAAAA TGATTTTGCA GAGAAGGAGA GAAAGCTGCA AGAGACACAG ATGTCCACCA 
     1621 CCTCAAAACT GGAGGAAGCT GAGCACAAAC TCCAGACTCT GCAAACAGCC CTGGAAAAAA 
     1681 CTCGAACAGA ATTATTTGAC CTGAAAACCA AATATGATGA AGAAACTACT GCAAAGGCCG 
     1741 ATGAGATCGA GATGATCATG ACCGACCTTG AACGAGCCAA CCAGAGGGCA GAGGTGGCAC 
     1801 AGAGAGAAGC AGAGACTTTA AGGGAACAGC TCTCATCGGC CAACCACTCT CTCCAACTGG 
     1861 CCTCGCAGAT CCAGAAGGCT CCAGATGTGG CCATAGAGGT GCTGACCCGA TCCAGCCTAG 
     1921 AAGTAGAGTT GGCTGCCAAA GAGCGGGAGA TCGCCCAGCT GGTGGAAGAT GTGCAGCGAC 
     1981 TCCAGGCCAG CCTCACCAAG CTACGTGAGA ATTCCGCCAG CCAGATCTCA CAGCTGGAGC 
     2041 AGCAACTGAA TGCCAAGAAT AGCACACTCA AACAACTGGA AGAAAAACTC AAAGGCCAGG 
     2101 CTGACTATGA AGAAGTGAAG AAAGAGCTGA ACACCCTGAA GTCCATGGAG TTTGCACCAT 
     2161 CGGAGGGAGC AGGGACACAG GACTCTACCA AGCCCCTGGA GGTTTTACTC CTGGAGAAGA 
     2221 ACCGCTCGCT GCAGTCCGAG AATGCCACGC TGCGCATCTC CAACAGTGAC CTGAGCGGGT 
     2281 CAGCCAGGAG AAAAGGGAGA GACCAGCCTG AGAGTCGGCG CCCGGGACCC TTGCCGGCCT 
     2341 CCCCTCCTCC TCAGTTGCCC CGCAACACGG GGGAACAGGT TTCCAATACT AACGGTACAC 
     2401 ACCACTTCTC ACCAGCGGGG TTAAGTCAAG ACTTTTTCAG CTCAAACCTG GCCAGCCCCA 
     2461 GCCTACCCCT GGCTTCTACA GGAAAGTTTG CACTAAACTC TCTTCTCCAG CGACAGCTAA 
     2521 TGCAGTCCTT CTACTCCAAG GCCATGCAGG AAGCCGGAAG CACAAGCACC ATTTTTTCAA 
     2581 CAGGTCCTTA CAGCACAAAC TCCATATCTT CCCCAAGTCC ATTACAACAA AGCCCAGATG 
     2641 TAAATGGCAT GGCCCCATCT CCCAGCCAAT CAGAAAGTGC TGGGAGCATC TCAGAGGGCG 
     2701 AGGAAATTGA TACCGCAGAG ATCGCCCGGC AGGTCAAAGA GCAGCTCATC AAGCACAACA 
     2761 TTGGCCAGCG CATCTTCGGA CATTACGTCC TGGGACTGTC GCAAGGCTCT GTGAGCGAGA 
     2821 TCCTGGCCCG GCCCAAGCCC TGGAACAAGC TGACTGTCCG TGGCAAGGAG CCATTCCACA 
     2881 AGATGAAGCA GTTCCTGTCT GATGAGCAGA ACATCCTGGC ACTCCGTAGC ATCCAAGGCA 
     2941 GACAGAGAGG TAACATCACT ACCCGGATCC GAGCATCTGA GACTGGTTCT GATGAAGCCA 
     3001 TCAAGTCCAT CCTGGAACAA GCCAAGAGGG AACTACAAGT GCAGAAAACC GCTGAGCCAG 
     3061 TCCAGACATC TTCCACGTCC AGCAGTGGGA ACTCTGACGA TGCCATCCGC TCCATCCTAC 
     3121 AGCAGGCCCG CCGGGAAATG GAGGCCCAGC AGGCTGCCCT TGACCCTGCC TTAAAGCCAG 
     3181 CACCACTGTC CCAGCCCGAC CTCACCATCC TCACCCCCAA GCACCTGTCT GCCTCACCCA 
     3241 TGTCTACTGT GTCCACTTAC CCGCCTCTTG CTATCTCCCT GAAGAAAACC CCTGCAGCAC 
     3301 CTGAGACCAG TACCGCAGCC TTGCCCAGTG CCCCAGCTCT CAAAAAAGAG GCTCAGGATG 
     3361 TACCCACACT GGACCCACCA GGATCAGCTG ATGCTGCCCA GGGAGTGCTG AGGCCGATGA 
     3421 AGAGTGAGCT AGTCCGTGGC AGCACCTGGA AGGATCCCTG GTGGAGCCCT ATACAGCCTG 
     3481 AAAGAAGAAA CCTCACTTCT TCCGAAGAGA CCAAGGCCGA TGAAACAACT GCAAGCGGGA 
     3541 AAGAAAGGGC AGGCAGCAGC CAGCCTCGGG CTGAGCGCAG CCAGCTTCAG GGACCCTCAG 
     3601 CATCCGCAGA GTACTGGAAA GAGTGGCCAA GCGCTGAGTC CCCATACTCC CAGAGCTCAG 
     3661 AGCTGAGCCT CACTGGAGCC AGCCGCAGTG AGACACCCCA GAATAGCCCT CTGCCCTCTT 
     3721 CCCCAATTGT GCCCATGGCG AAGCCAGCCA AGCCTTCAGT GCCCCCGCTG ACTCCTGAGC 
     3781 AGTACGAGGT CTACATGTAT CAGGAAGTGG ACACTATCGA GCTCACTCGG CAGGTCAAAG 
     3841 AGAAGCTGGC CAAGAACGGC ATTTGCCAGA GGATCTTTGG GGAGAAGGTA CTGGGCCTGT 
     3901 CCCAGGGCAG TGTCAGCGAC ATGCTTTCAA GGCCAAAACC ATGGAGCAAA CTGACCCAGA 
     3961 AAGGCCGAGA ACCCTTCATC CGGATGCAGC TCTGGCTGAA TGGAGAGCTG GGCCAAGGTG 
     4021 TTCTGCCAGT GCAAGGGCAA CAGCAAGGGC CAGTCCTCCA CTCCGTGGCG TCTCTGCAGG 
     4081 ACCCCCTCCA GCAGGGCTGT GTGAGCTCAG AAAGCACTCC AAAGACCTCT GCCAGCTGCA 
     4141 GCCCTGCCCC TGAGTCCCCA ATGAGTTCCA GCGAATCTGT GAAGAGTCTC ACCGAGCTGG 
     4201 TCCAGCAGCC CTGTCCCGCC ATTGAGACCA GTAAAGAAGG CAAACCACCA GAGCCCAGCG 
     4261 ACCCGCCTGC CTCAGACTCC CAGCCCACAA CACCGCTGCC TCTCTCTGGA CACTCAGCCC 
     4321 TCAGCATCCA AGAATTAGTA GCCATGTCTC CTGAGCTGGA CACGTATGGC ATAACCAAGA 
     4381 GGGTCAAAGA GGTGCTGACA GACAACAACC TCGGTCAGCG CTTATTTGGG GAGACCATTT 
     4441 TGGGGCTCAC CCAGGGTTCT GTCTCCGACC TCCTTGCCCG CCCAAAGCCT TGGCATAAGC 
     4501 TTAGTCTGAA AGGACGGGAA CCCTTTGTCC GCATGCAGCT ATGGCTGAAC GACCCCAACA 
     4561 ATGTGGAGAA GCTGATGGAT ATGAAGCGGA TGGAGAAGAA AGCCTACATG AAGAGGCGAC 
     4621 ACAGCTCTGT CAGTGACAGT CAGCCTTGTG AGCCCCCCTC TGTGGGTATT GACTATAGCC 
     4681 AAGGCGCCAG CCCCCAGCCA CAACACCAGC TGAAGAAACC TCGAGTGGTG CTGGCTCCAG 
     4741 AGGAGAAGGA AGCGCTGAAG CGAGCATATC AGCAGAAGCC ATACCCATCA CCAAAAACCA 
     4801 TCGAGGAACT TGCCACACAA CTCAACCTGA AGACCAGCAC CGTCATCAAC TGGTTCCATA 
     4861 ATTACAGGTC TCGGATCCGC AGAGAACTGT TCATTGAGGA GATTCAAGCT GGAAGCCAGG 
     4921 GCCAGGCTGG TGCCAGCGAC TCACCTTCAG CTCGAAGTAG CCGCGCTGCA CCCAGCTCGG 
     4981 AAGGTGACAG CTGTGACGGC GTGGAAGCCA CCGACGCCGA GGAGCCAGGT GGCAACATAG 
     5041 TGGCCACCAA GTCTCAGGGA GGGCTGGCAG AGGTGGCCGC AGCCCCGGCA GACCGGGAAG 
     5101 AAGCGACGCA GCCGGCGGAG AAGGCGAAGG CACAGCCACT ATGCTCGGGG ACCCCAGGAC 
     5161 AGGACGACGG TGAAGACGCA AGCCGGCCTA GGCCACTTCC CGAGGGCCTC GCCGACGCCC 
     5221 CAGCGCCTGT GCCAAGCCTC GCCGCACCCG CGGCCGGAGA GGACGCCGCT ACCTCAGCCA 
     5281 CTGCCCCGGC CACGGCCACC GAGGCCCCCG GGGCGGCCAG GGCGGGCCCT GCCGAGAGGA 
     5341 GTTCTGCGTT GCCAAGCACC AGCGCGCCTG CCAACGCGCC TGCGCGAAGG CCTAGCTCAC 
     5401 TGCAGAGCCT CTTCGGTCTG CCCGAGGCCG CGGGCGCCCG GGACAACCTG GTGCGGAAGA 
     5461 AGAAGGCTGC GAACTTGAAC AGCATCATCC ACCGCCTGGA GAAGGCTGCC AGCCGGGAGG 
     5521 AGCCCATCGA ATGGGAGTTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5581 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5641 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5701 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5761 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5821 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5881 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5941 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6001 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6061 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6121 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6181 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6241 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6301 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6361 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6961 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7021 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7081 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7141 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7201 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7261 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7321 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7381 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7441 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7501 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7561 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7621 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7681 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7741 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7801 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7861 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7921 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7981 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8041 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8101 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8161 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8221 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8281 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8341 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8401 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8461 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8521 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8581 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8641 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8701 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8761 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8821 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8881 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8941 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9001 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9061 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9121 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9181 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9241 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9301 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9361 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//