LOCUS       MR231822                9390 bp    DNA     circular  SYN 
DEFINITION  Nrxn2 (myc-DDK-tagged) - Mouse neurexin II (Nrxn2), transcript variant 3
ACCESSION   NM_001205235
VERSION     NM_001205235.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5547..5638 
                     /label="Myc-DDK"  
     misc_feature    5668..6253 
                     /label="PolyA signal"  
     misc_feature    5725..5744 
                     /label="Restriction Enzyme"  
     misc_feature    6529..7201 
                     /label="ColE1"  
     misc_feature    7631..8425 
                     /label="Kan/Neo"  
     misc_feature    8460..8762 
                     /label="SV40 ori"  
     misc_feature    8848..9325 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5529..5535 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5529 
                     /label="Nrxn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTCGGG AGTCGGTGGC AACCGCCACC CCAGCTGCCG CCGCTGCTGT 
     1081 TGCTGCTGGC GCTGGCGGCA GGCGTCCGTG GCTTGGAGTT CGGCGGCGGC CCCGGGCAGT 
     1141 GGGCTCGCTA CGCGCGTTGG GCGGGAGCGG CGAGCACCGG CGAGCTCAGC TTCAGCCTGC 
     1201 GCACCAACGC CACGCGCGCG CTGCTGCTCT ACCTGGACGA CGGCGGCGAC TGCGACTTCT 
     1261 TGGAGCTGCT GCTGGTGGAC GGGCGCCTGC GGCTGCGCTT CACGCTGTCT TGCGCGGAGC 
     1321 CCGCCACGCT GCAGCTGGAC ACGCCGGTGG CCGACGACCG CTGGCACATG GTGTTGCTGA 
     1381 CCCGCGACGC GCGGCGCACG GCGCTGGCGG TGGACGGCGA AGCCCGCGCC GCCGAGGTCC 
     1441 GCTCAAAGCG GCGCGAGATG CAGGTGGCCA GCGACCTGTT CGTGGGCGGC ATCCCTCCCG 
     1501 ACGTGCGCCT ATCTGCACTC ACGCTCAGCA CCGTCAAGTA CGAGCCGCCT TTCCGCGGCC 
     1561 TTCTGGCCAA CCTGAAGCTG GGCGAGCGGC CGCCGGCGCT GCTGGGTAGC CAGGGTCTGC 
     1621 GCGGTGCGGC CGCCGACCCT CTGTGTGCGC CTGCGCGCAA TCCCTGCGCC AACGGCGGTC 
     1681 TCTGCACCGT GCTAGCCCCC GGCGAGGTGG GCTGCGACTG CAGCCACACT GGATTTGGCG 
     1741 GCAAGTTCTG CAGTGAAGAG GAACACCCCA TGGAAGGTCC GGCTCACCTG ACGTTAAACA 
     1801 GCGAAGTAGG GTCCTTACTG TTCTCCGAGG GGGGGGCCGG GAGAGGAGGA GCCGGCGATG 
     1861 TGCACCAGCC GACAAAAGGC AAGGAGGAAT TTGTGGCAAC CTTCAAGGGC AATGAGTTCT 
     1921 TCTGCTACGA CCTGTCCCAC AACCCGATCC AGAGCAGCAC TGACGAGATC ACACTGGCCT 
     1981 TCCGCACCCT GCAGCGCAAC GGGCTGATGC TGCACACGGG GAAGTCGGCT GACTACGTCA 
     2041 ACCTGTCCCT CAAGTCTGGG GCTGTCTGGC TGGTCATCAA CCTAGGCTCA GGTGCCTTCG 
     2101 AGGCCCTCGT GGAACCCGTC AATGGCAAGT TCAACGACAA CGCCTGGCAC GACGTCCGGG 
     2161 TTACCCGAAA CCTGCGCCAG GTGACCATCT CGGTGGACGG GATCCTGACC ACCACAGGCT 
     2221 ACACGCAGGA GGATTACACC ATGCTGGGCT CTGATGACTT CTTCTACATT GGGGGCAGCC 
     2281 CCAACACAGC CGACCTGCCT GGCTCACCTG TCAGCAACAA CTTCATGGGC TGCCTCAAGG 
     2341 ACGTGGTCTA CAAGAATAAT GACTTCAAGC TGGAGCTGTC CCGACTGGCT AAGGAAGGGG 
     2401 ACCCGAAGAT GAAGCTGCAG GGGGATTTGT CTTTCCGTTG TGAGGATGTG GCTGCCTTGG 
     2461 ACCCTGTGAC CTTTGAGAGT CCTGAGGCCT TTGTCGCACT GCCCCGCTGG AGCGCCAAGC 
     2521 GCACTGGTTC CATCTCCCTG GACTTCAGAA CCACTGAGCC CAATGGGTTG TTGCTCTTCA 
     2581 GCCAGGGCCG GCGGGCTGGG GCCGGGGTAG GCAGTCACAG TTCTACCCAG AGGGCCGACT 
     2641 ACTTTGCCAT GGAGCTGTTG GATGGCTACC TCTACCTTCT GCTGGACATG GGCTCCGGGG 
     2701 GCATCAAGCT GCGGGCGTCT AGCCGCAAGG TCAATGATGG TGAATGGTGC CACGTGGACT 
     2761 TCCAGAGGGA CGGGCGCAAA GGCTCCATCT CTGTGAACAG CCGCAGCACG CCATTCTTGG 
     2821 CCACAGGAGA GAGCGAGGTC CTGGACCTGG AGAGTGAGCT GTACCTGGGC GGTCTCCCCG 
     2881 AGGGGGGACG AGTGGACCTG CCACTGCCCC CTGAGGTGTG GACAGCTGCT CTCCGGGCTG 
     2941 GCTACGTGGG CTGTGTGAGA GACCTCTTCA TCGATGGACG GAGTCGAGAT CTCCGGGGCC 
     3001 TGGCTGAGGC CCAGGGGGCT GTGGGCGTTG CTCCTTTCTG CTCCCGGGAG ACCCTGAAGC 
     3061 AGTGTGCGTC GGCCCCTTGT CGAAACGGGG GCATCTGTCG AGAGGGCTGG AACCGGTTCG 
     3121 TCTGTGACTG CATCGGGACC GGCTTTCTGG GTCGGGTCTG CGAGAGAGAG GCCACGGTCT 
     3181 TAAGCTATGA CGGCTCCATG TACATGAAGA TCATGCTGCC CACTGCGATG CACACGGAAG 
     3241 CAGAGGATGT GTCCTTACGC TTCATGTCTC AGAGGGCATA TGGACTCATG ATGGCCACCA 
     3301 CCTCCAGGGA GTCGGCTGAC ACTCTGCGTC TCGAGCTGGA CGGGGGGCAG ATGAGGCTCA 
     3361 CCGTCAACCT CGACTGCCTG CGCGTCGGCT GCGCACCCAG TAAAGGCCCC GAGACACTGT 
     3421 TTGCGGGGCA CAAGCTGAAT GACAATGAAT GGCACACGGT GAGGGTGGTC CGGCGTGGCA 
     3481 AGAGCCTGCA GCTGTCCGTG GACAACGTGA CTGTGGAGGG ACAGATGGCA GGAGCCCACA 
     3541 CTCGGCTGGA GTTCCACAAC ATTGAGACGG GCATTATGAC AGAGCGGCGG TTTATCTCTG 
     3601 TGGTGCCCTC CAACTTCATC GGGCACCTGA GTGGGCTGGT GTTCAATGGT CAACCCTACA 
     3661 TGGACCAATG CAAAGATGGA GACATCACCT ATTGTGAGCT TAATGCCCGC TTTGGCCTGC 
     3721 GTGCCATCGT GGCTGATCCT GTCACGTTCA AGAGTCGCAG CAGCTACCTG GCGTTAGCCA 
     3781 CGCTGCAAGC CTATGCTTCC ATGCATCTCT TCTTCCAGTT CAAGACCACG GCCCCAGATG 
     3841 GACTTCTGCT GTTCAACTCA GGCAACGGCA ACGACTTCAT CGTCATCGAG TTGGTCAAGG 
     3901 GGTACATCCA CTACGTGTTT GACCTGGGAA ATGGCCCGTC CTTGATGAAG GGAAACTCAG 
     3961 ACAAACCAGT CAATGACAAC CAATGGCACA ATGTGGTGGT GTCCAGGGAC CCAGGCAACG 
     4021 TGCACACACT GAAGATCGAC TCCCGCACAG TCACGCAGCA TTCCAACGGT GCCCGAAATC 
     4081 TGGATCTCAA AGGGGAGTTG TATATCGGTG GCCTGAGCAA GAATATGTTC AGCAACCTGC 
     4141 CCAAGCTGGT GGCCTCTCGG GATGGCTTTC AAGGCTGCTT GGCTTCTGTG GACCTCAACG 
     4201 GACGCCTCCC AGACCTCATC GCTGACGCCC TGCACCGCAT CGGGCAGGTG GAGAGGGGCT 
     4261 GTGATGGCCC TAGCACCACC TGCACCGAAG AGTCTTGTGC CAACCAGGGC GTCTGTCTGC 
     4321 AGCAGTGGGA TGGTTTTACC TGTGACTGTA CTATGACTTC CTATGGAGGC CCTGTCTGCA 
     4381 ATGACCCTGG GACCACATAC ATCTTCGGGA AGGGGGGAGC GCTCATCACC TATACATGGC 
     4441 CTCCCAATGA CCGGCCCAGT ACACGGATGG ACCGCCTGGC CGTAGGCTTC AGCACACACC 
     4501 AGCGGAGCGC TGTGCTGGTG CGAGTGGACA GTGCCTCCGG CCTCGGGGAC TACTTGCAGC 
     4561 TGCACATTGA CCAGGGCACT GTTGGGGTGA TTTTTAATGT GGGCACGGAC GACATTACAA 
     4621 TTGATGAGCC CAACGCCATC GTGAGCGACG GCAAATATCA CGTGGTGCGC TTTACTCGAA 
     4681 GTGGTGGCAA TGCCACCTTG CAAGTGGACA GCTGGCCAGT CAACGAGAGG TACCCGGCAG 
     4741 GAAACTTTGA TAACGAGCGC CTGGCGATTG CTAGACAGAG AATCCCCTAC CGGCTTGGTC 
     4801 GAGTAGTAGA TGAGTGGCTG CTCGACAAAG GACGCCAGCT GACCATCTTC AACAGCCAGG 
     4861 CTGCCATCAA GATAGGGGGC CGGGATCAGG GCCGCCCCTT CCAGGGCCAG GTGTCCGGCC 
     4921 TCTACTACAA TGGGCTCAAG GTACTGGCGC TGGCCGCCGA GAGCGACCCC AATGTGCGGA 
     4981 CCGAAGGCCA CCTACGGCTA GTAGGGGAGG GGCCGTCCGT GCTGCTCAGT GCTGAGACCA 
     5041 CTGCCACCAC TCTGCTGGCC GACATGGCCA CCACCATCAT GGAGACCACC ACCACCATGG 
     5101 CCACCACCAC TACTCGCCGG GGCCGTTCCC CCACAATGAG GGACAGCACC ACCCAGAACA 
     5161 CAGATGACCT CCTGGTGGCC TCGGCTGAGT GTCCAAGTGA TGATGAGGAC CTAGAGGAGT 
     5221 GTGAGCCTAG TACTGCCAAC CCCACGGGTC CGGGGGAGCG CGGCCCGCCA GGTGCAGTGG 
     5281 AGGTGATCCG CGAATCCAGC AGCACCACGG GCATGGTGGT GGGCATCGTG GCGGCGGCGG 
     5341 CGCTCTGCAT CCTCATTCTC CTCTACGCCA TGTACAAGTA CCGCAACCGC GACGAGGGCT 
     5401 CCTACCAGGT GGACCAGAGC CGGAATTACA TCAGTAACTC GGCCCAGAGC AATGGGGCGG 
     5461 TGGTGAAGGA GAAGGCCCCT GCTGCCCCCA AGACGCCAAG CAAGGCCAAG AAGAACAAAG 
     5521 ACAAAGAGTA TTACGTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5581 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5641 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5701 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5761 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5821 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5881 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5941 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6001 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6061 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6121 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6181 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6241 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6301 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7201 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7261 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7321 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7381 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7441 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7501 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7561 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7621 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7681 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7741 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7801 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7861 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7921 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7981 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8041 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8101 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8161 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8221 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8281 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8341 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8401 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8461 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8521 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8581 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8641 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8821 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8941 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9361 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//