LOCUS       MR231810                9291 bp    DNA     circular  SYN 
DEFINITION  Magi1 (myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 5
ACCESSION   NM_001286784
VERSION     NM_001286784.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5448..5539 
                     /label="Myc-DDK"  
     misc_feature    5569..6154 
                     /label="PolyA signal"  
     misc_feature    5626..5645 
                     /label="Restriction Enzyme"  
     misc_feature    6430..7102 
                     /label="ColE1"  
     misc_feature    7532..8326 
                     /label="Kan/Neo"  
     misc_feature    8361..8663 
                     /label="SV40 ori"  
     misc_feature    8749..9226 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5430..5436 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5430 
                     /label="Magi1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAAGTG ATCCAGAAGA AGAACCACTG GACTGGCCGC GTTCACGAGT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTCCTGGGG GGCGCGGAGC 
     1141 ATGGGGAGTT TCCGTACGTG GGGGCGGTGG CGGCGGCCGA GGCGGCGGGG CTTCCCGGCG 
     1201 GTGGCGAGGG GCCGAAGCTG GCCGAAGGTG AGCTGCTGCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GAGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAAGC CGTCAGACAA GGAGGAAGGC TCAACAAGGA CCTACGACAT TTCCTCAACC 
     1381 AACGGTTCCA GAAGGGGTCT CCAGATCATG AGCTCCAGCA GACCATAAGG GACAACCTCT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGGT CTCCCAGAGA AGGAGAAGTG CCTGGTGTGG 
     1501 ATTACAGCTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGCGGG ACCCTGTTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA ACCTCCTAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTGCA GTCTGGCTCC AAGCAGTCGA 
     1681 CCCCTAAGCG AACAAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC CACCCGGAGA 
     1741 ATGAGGAGGA GGAGGATGTC CCTGAAATGA ACAGTAGCTT TACAGCCGAC TCTGGAGACC 
     1801 AGGACGAGCA CACTCTCCAA GAAGCAACGC TCCCGCCTGT GAATAGTAGC ATCCTCGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAGTACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TCATAGACCA CAACACGAAA ACAACATCAT GGTTAGACCC TCGGTGCCTG AACAAACAGC 
     2041 AGAAGCCTCT GGAAGAATGT GAAGATGATG AGTTGCCTGC TGGCTGGGAA AAGATTGAAG 
     2101 ACCCTGTCTA CGGTGTCTAC TATGTAGACC ACATCAACAG GAAGACGCAA TATGAAAACC 
     2161 CAGTCCTAGA AGCCAAACGG AAGAAACAGC TTGAACAGCA GCAGCAACAG CAGCAGCCTC 
     2221 AGCCACCGCA GCCAGAAGAG TGGACAGAGG ATCATGCATC TGTTGTGCCT CCTGTTGCTC 
     2281 CTTCCCATCC CCCGAGCAAT CCGGAGCCAG CCAGGGAAAC TCCACTTCAG GGCAAACCTT 
     2341 TTTTTACAAG AAACCCCTCT GAGCTGAAAG GCAAGTTCAT TCACACGAAG CTACGGAAAA 
     2401 GCAGCCGAGG CTTTGGCTTC ACGGTGGTTG GAGGAGACGA GCCTGATGAG TTCCTGCAGA 
     2461 TCAAGAGCCT CGTCCTCGAT GGTCCTGCCG CACTGGATGG CAAGATGGAG ACAGGGGATG 
     2521 TAATTGTGAG TGTGAATGAC ACCTGTGTTT TGGGACACAC ACATGCTCAA GTTGTGAAAA 
     2581 TCTTCCAGTC CATTCCCATT GGTGCCAGTG TGGACCTTGA ACTCTGCAGA GGTTATCCAT 
     2641 TGCCTTTTGA CCCGGATGAC CCTAATACAA GTTTAGTGAC CTCGGTGGCC ATTTTGGACA 
     2701 AAGAACCAAT TATTGTAAAT GGACAAGAGA CCTACGATTC ACCAGCGAGC CACAGTAGTA 
     2761 AAACAGGCAA AGTCAGCAGC ATGAAGGATG CCAGGCCAAG CAGCCCTGCT GATGTGGCTT 
     2821 CCAACAGCTC TCATGGTTAT CCCAACGACA CAGTCTCCTT GGCTTCCTCC ATAGCCACCC 
     2881 AGCCAGAGCT AATAACTGTT CACATAGTCA AAGGGCCAAT GGGATTTGGC TTTACGATCG 
     2941 CAGACAGTCC CGGTGGGGGT GGCCAAAGAG TGAAACAGAT TGTTGACAGT CCACGCTGCA 
     3001 GAGGCCTCAA AGAAGGGGAT CTTATCGTGG AGGTGAATAA GAAGAACGTG CAGGCCCTGA 
     3061 CGCACAATCA AGTCGTGGAT ATGCTGATTG AATGTCCCAA GGGAAGTGAG GTCACACTGT 
     3121 TGGTGCAGCG AGGAGGGCTA CCAGTTCCCA AGAAGAGCCC AAAGTCGCAG CCACTGGAGA 
     3181 GGAAAGACAG CCAGAATAGC TCCCAGCACA GCGTCTCCAG CCACCGGAGC CTGCACACTG 
     3241 CGTCCCCGAG CCACGGCATA CAGGTGCTCC CTGAGTACCT ACCTGCAGAC GCCCCTGCTC 
     3301 CAGATCAGAC CGACAGCTCT GGGCAGAAAA AGCCAGATCC TTTTAAAATC TGGGCCCAGT 
     3361 CCAGGAGCAT GTATGAAAAC CGACCTATGT CACCTTCGCC TGCATCAGGA TTGAGCAAGG 
     3421 GTGAAAGAGA CAGAGAAATC AATTCCACGA ATTTTGGAGA ATGTCAGATT CCAGATTACC 
     3481 AGGAACAGGA CATCTTCCTC TGGAGAAAAG AAACCGGATT TGGATTTAGG ATTCTGGGTG 
     3541 GAAATGAACC AGGGGAACCC ATTTATATCG GTCACATCGT ACCGCTGGGT GCTGCTGACA 
     3601 CAGACGGCCG CCTGAGGTCT GGAGATGAAT TAATCTGTGT GGATGGGACA CCAGTAATTG 
     3661 GGAAATCACA CCAGCTCGTG GTCCAGCTTA TGCAACAAGC TGCCAAGCAA GGCCATGTCA 
     3721 ATCTCACAGT GAGGCGGAAA GTGGTCTTTG CCGTCCCCAA AGCAGAGAAT GAGGTGCCCT 
     3781 CACCAGCCTC ATCACACCAC AGTAGCAACC AGCCCGCGTC CCTGACGGAG GAGAAACGCA 
     3841 CACCGCAAGG CAGCCAGAAC TCTCTGAACA CTGTGAGCTC TGGCAGCGGC AGCACCAGTG 
     3901 GCATTGGCAG TGGTGGCGGC GGGGGCAGCG GTGTGGTGAG CGCTGTGCTG CAGCCCTATG 
     3961 ATGTGGAGAT TCGGCGTGGG GAGAACGAGG GCTTTGGGTT TGTCATCGTG TCCTCCGTGA 
     4021 GCAGACCCGA AGCGGGCACA ACCTTCGGCA ATGCATGTGT GGCTATGCCT CACAAAATAG 
     4081 GTCGGATTAT TGAGGGGAGC CCTGCTGACC GCTGTGGCAA GCTGAAAGTA GGAGACCGGA 
     4141 TCTTGGCAGT AAATGGATGT TCCATCACCA ACAAATCCCA TTCTGACATT GTCAACCTAA 
     4201 TCAAAGAAGC GGGCAACACA GTGACTCTCC GCATCATCCC CGGGGATGAG TCCTCAAATG 
     4261 CCACGCTGCT GACTAATGCT GAGAAGATTG CCACCATCAC CACCACTCAT GCCCCCTCTC 
     4321 AGCAGGGGAC CCAGGAAACA AGGACCACCA CCAAACCAAA GCAGGATTCT CAGTTTGAGT 
     4381 TCAAAGGACC GCAGGCTGCA CAGGAGCAAG ATTTCTACAC TGTGGAATTG GAAAGAGGGG 
     4441 CCAAGGGATT TGGCTTTAGT CTTCGAGGGG GCCGAGAATA TAACATGGAT CTTTATGTTC 
     4501 TGCGCTTGGC AGAGGATGGT CCTGCAGAAA GATGTGGGAA GATGAGGATT GGCGATGAAA 
     4561 TTCTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAACA CTCTCGGGCC ATAGAACTGA 
     4621 TCAAGAATGG CGGCCGCAGG GTCCGTCTGT TTCTGCGGCG GGGAGACGGC TCAGTCCCAG 
     4681 AATATGACCC CAGCAGCGAC AGGAACGGCC CCTCCACCGG TGCACAAGGT GTTCCGGAAG 
     4741 TGAGGCCGGG GCCGCCAGAC CACAGACCGC ATCCAGCCTT GGAGTCCAGT TACCCACCCG 
     4801 AACTTCACAA ATCATCACAA CATGCCGAAA AGCGAGCACA CGCGAAGGAT CCAAAAGGCA 
     4861 ACAGGGAGCA CAGCAAACAA CCCAACGAAC ATCACACCTG GAATGGAACT TCTAGAAAAC 
     4921 AGGACAGCGG GGCATGCCGG CCCAAAGACC GGCCACCCGA CGCATGGAGA GAGGCGCAGC 
     4981 CAGAGCGGAC AGCCACCAAT GGTTCAAAGA GGAGGTCACC GGAGAAGCGC AGGGAAGGCA 
     5041 CCCGCAGCGC TGACAACACT TTGGAAAGAA GGGAGAAGCA TGAGAAGAGA AGAGAGATAT 
     5101 CTCCCGAGAG GAAGCGAGAG CGTTCACCCA CCCGGAGAAA AGATAGCTCC CCGAGCCGCC 
     5161 GGCGGAGGTC CCTCGAAAGA CTCCTGGATC AAAGACGGTC CCCAGAGCGC AGAAGAGGGG 
     5221 GCTCCCCCGA GAGGAGAGCC AAATCCACCG ACAGGAGGAG GGCCAGGTCC CCTGAGCGCA 
     5281 GGCGAGAGCG GTCACTGGAC AAAAGGAACC GGGACGACAA GGTTGGCCAC CGAGAAAGAG 
     5341 AGGAGGCTGG TCTGAAGCTG GAAGCGGGGA GAAGCCCCCG AAATCCCCCA GAGCAGAGAA 
     5401 GGCGGCCTTA CAAAGAATGT AGCACCGACC TCAGCATCAC GCGTACGCGG CCGCTCGAGC 
     5461 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5521 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5581 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5641 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5701 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5761 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5821 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5881 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5941 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6001 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6061 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6121 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6181 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6241 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6301 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6361 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6421 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6481 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6541 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6601 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6661 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6721 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6781 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6841 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6901 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6961 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7021 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7081 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7141 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7201 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7261 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7321 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7381 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7441 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7501 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7561 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7621 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7681 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7741 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7801 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7861 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7921 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7981 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8041 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8101 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8161 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8221 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8281 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8341 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8401 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8461 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8521 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8641 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8701 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8761 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8821 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8881 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8941 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9001 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9061 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9121 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9181 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9241 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//