LOCUS       MR231809                9273 bp    DNA     circular  SYN 
DEFINITION  Eif4g3 (myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4 gamma, 3 (Eif4g3), transcript variant 3
ACCESSION   NM_001256198
VERSION     NM_001256198.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="Eif4g3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTCACAA CCTCAGGCCC GGTCTCCGGG AGGATTCAGA CCCATCCAGT 
     1081 TTTTCCAGAG GCCTCAAATC CAGCCTCCCC GAGCTGCCAT CCCAAACAGC AGTCCTTCCA 
     1141 TCCGTCCCGG TGTGCAGACC CCCACCGCCG TGTATCAGGC CAACCAGCAC ATCATGATGG 
     1201 TTAACCATCT GCCCATGCCG TACCCCGTGA CCCAGGGGCA TCAGTACTGC ATACCACAGT 
     1261 ACCGCCACAG CGGCCCTCCG TACGTGGGGC CCCCACAGCA GTATCCAGTT CAGCCGCCTG 
     1321 GGCCGGGGCC TTTTTATCCT GGACCAGGAC CTGGGGACTT CGCTAATGCT TATGGAACGC 
     1381 CTTTTTACCC AAGTCAGCCA GTGTATCAGT CAGCACCTAT CATAGTGCCT ACGCAGCAGC 
     1441 AGCCCCCTCC AGCCAAGAGA GAGAAAAAAA CTATAAGAAT TCGAGATCCA AATCAGGGAG 
     1501 GTAAAGACAT AACAGAGGAG ATTATGTCTG GAGGTGGCAG CAGAAATCCC ACTCCACCTA 
     1561 TAGGAAGACC CGCATCCACT CCCACTCCTC CTCAGCAGCT GCCCAGCCAG GTCCCTGAGC 
     1621 ACAGCCCTGT GGTATATGGG ACTGTGGAGA GCGCTCATCT TGCTGCCAGC ACCCCTGTCA 
     1681 CTGCAGCTAG CGACCAGAAG CAAGAAGAAA AGCCAAAACC AGATCCAGTG TTCCAGTCTC 
     1741 CTTCGACGGT CCTTAGGCTC GTACTCAGTG GGGAGAAGAA AGAGCAAGCA GGCCAGATGC 
     1801 CTGAGACTGC TGCAGGAGAG CCCACCCCAG AGCCTCCTCG GACTTCATCA CCCACCAGCC 
     1861 TTCCTCCACT TGCCCGGAGT TCACTTCCTT CCCCCATGTC CGCTGCCCTT AGCAGCCAGC 
     1921 CACTGTTCAC TGCTGAGGAC AAGTGTGAAC TTCCTTCCTC CAAAGAAGAA GATGCACCTC 
     1981 CTGTCCCCAG CCCCACGTCT TGCACAGCAG CCTCAGGCCC TTCACTAACA GATAACAGTG 
     2041 ATATATGCAA GAAACCCTGT AGCGTAGCGC CTCATGATAG TCAGCTAATT TCTAGTACTA 
     2101 TCCTAATTAA TGAAATGAAT GGAGTTGGAG AAAAATTATC AGCCAAGGAG AACACTGTGG 
     2161 GCATGCTGAG ACAGGAAGTG CTGCCATTGA CTCTCGAGTT AGAGATTCTG GAACATCCAC 
     2221 AGGAAGAATT GAAAGTGGAA TGTACGCCGA CTCCCATTGC CCCGTCCATG CTGCCTGCCT 
     2281 TCTCCCCAGC TCCTCCGACT CCCCCCACTT CTCCTCCTTG TCCTCCGGTT GTCCTCTCTG 
     2341 CCGCCATTGC TCGTTCTCCC GCTGTTGCCA CTGAGGTCCA GAGAGTCGCC GACGAGGGAG 
     2401 AAAGCCTGAG AACTTGCCTT AGCAAGGATG CAAAGGAGAT GCAGGACAAA GCAGAGTCAG 
     2461 AATCAGATGG GCAAGCAGAA GAGACTGCGG ATCCCCAGAG CCTACATTCA GGAAGGAGCC 
     2521 CCGCCCCAGT GCAAACAGCT ACCACTGCGC CAAAGTCCTG GAAGAAAACA AAAGAGCAGA 
     2581 CCCGAACCCC AGATGAAGTG TTAGAAGCAG AGGCAGAGCC TAAAGCCGAA GAAGAGCTTG 
     2641 CAGTTGACAG TGTCCTTGAG CCTGAGCAAG AGAAGATGAG CCAAGGGTTT CCGTCTGAGA 
     2701 GAGACCCCTC TGCCCTGAAG AGAGGGAAAG CTGAGGAAGG AAACGGAGAA GAGGCTGAGC 
     2761 CTGTGCGAAA TGGCGCTGAG AGTGCTTCTG AGGGGGAAGG GGGAGATGGC AACTCAGGCT 
     2821 CTGCTGACAG CTCTGCTGAT GGGCTCACCT TCCCGTTTAA AGCAGAGTCC TGGAAGCCTG 
     2881 CAGATACAGA AGGCAAGAAG CAGTATGACC GGGAGTTCCT ACTGGACATC CAGTTTATGC 
     2941 CCGCCTGTAT CCAAAAACCA GAAGGCCTGC CTCCCATCAG CGACGTGGTC CTTGACAAGA 
     3001 TCAACCAGCC CAGATTGTCA ATGCGGACCC TGGATCCTCG CATTTTGCCT CGAGGACCTG 
     3061 ACTTCACCCC AGCCTTTGCA GACTTTCCAA GGCAAACGCC TGGTGGAAGA GGTGTGCCTC 
     3121 TGTTGAATGT TGGACCTCGG AGGTCTCAAC CTGGCCAGAG AAGGGAGCCC AGAAAGATAA 
     3181 TCACTGTCTC CGTAAAAGAA GATGTGCACT TGAGAAAGGC GGAGAATGCC TGGAAGCCGA 
     3241 GCCAGAAACG GGACAGCCAC GCCGATGACC CCGAAAGCAT TAAAACTCAG GAACTTTTCA 
     3301 GAAAAGTTCG AAGTATCTTG AATAAACTGA CACCACAGAT GTTCAACCAG CTGATGAAGC 
     3361 AAGTGTCGGC TCTTACGGTG GACACGGAGG AGCGGCTAAA AGGGGTCATC GACCTTGTCT 
     3421 TTGAGAAGGC TATCGATGAG CCCAGCTTCT CTGTGGCGTA CGCAAACATG TGTCGGTGCC 
     3481 TAGTAACGCT GAAGGTGCCC ATGGCAGACA AGCCTGGCAA CACAGTGAAT TTCCGGAAGC 
     3541 TGCTGCTTAA CCGCTGCCAG AAGGAGTTCG AGAAAGACAA AGCAGATGAT GACGTCTTTG 
     3601 AGAAGAAGCA GAAAGAACTG GAAGCTGCCA GCGCTCCAGA GGAGAGGACA AGGCTTCACG 
     3661 ACGAACTGGA AGAAGCCAAG GACAAAGCCC GGCGGAGATC CATCGGCAAC ATCAAGTTCA 
     3721 TTGGAGAACT CTTTAAACTG AAAATGCTGA CGGAGGCCAT CATGCATGAC TGTGTGGTGA 
     3781 AGCTCCTGAA GAACCACGAT GAAGAGTCCC TGGAGTGCCT GTGTCGCCTG CTCACCACCA 
     3841 TCGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGCAT GGACCAGTAC TTTAACCAGA 
     3901 TGGAGAAAAT CGTCAAAGAA AGAAAAACTT CCTCCAGAAT TCGGTTCATG CTTCAGGATG 
     3961 TAATCGACCT CAGACTGTGT AATTGGGTGT CCCGAAGAGC AGATCAGGGG CCAAAAACTA 
     4021 TCGAGCAGAT TCACAAAGAG GCCAAAATAG AAGAACAAGA GGAGCAGAGG AAAGTCCAGC 
     4081 AGCTGATGAC CAAGGAGAAG AGGAGACCAG GCGTCCAAAG AGTGGATGAA GGTGGATGGA 
     4141 ATACTGTACA AGGGGCCAAG AACAGTCGTG TACTGGACCC CTCCAAATTT TTGAAAATTA 
     4201 CTAAACCCAC CATTGATGAG AAGATTCAGC TGGTTCCTAA GGCACAGCTG GGCAGCTGGG 
     4261 GCAAAGGCAG CAGCGGGGGA GCAAAGGCAA GTGAGTCCGA TGCCTTACGG TCAAGTGCTT 
     4321 CCAGTTTAAA TAGATTCTCT CCTCTGCAAC CTCCAGCACC TTCAGGGTCC CCGTCGGCCA 
     4381 CACCCTTAGA GTTTGATTCC CGAAGGGCGT TAACCAGTCG TGGGAGCATG GGCAGGGAGA 
     4441 AGAGCGACAA GCCAATTCCA GCTGGAACAG CCCGTCCCAA CACGTTCCTG AGGGGCAGCA 
     4501 GCAAAGACCT GCTGGACAAC CAGTCCCAGG AAGAGCAGCG CAGAGAGATG TTGGAGACCG 
     4561 TGAAACAGCT GACAGGAGGC CTGGATGCTG AGCGGGCCAG CACTGAGGCC GACCGAAGCA 
     4621 AAACGAGGGA GCTAGCAAAA TCGGAAATGT GTGCAGTGCC AGCCCCTGAC AAGCCTGCAC 
     4681 TGTCAGAAGA GGAAGTGGAG AGGAAGTCCA AGTCTATCAT TGACGAATTC TTACACATCA 
     4741 ATGACTTTAA GGAGGCCACG CAGTGCATAG AGGAGCTGAG CGCCCAGGGC CCACTGCATG 
     4801 TGTTCGTGAA GGTGGGTGTG GAGTTCACCC TGGAACGGAG CCAGATCACC AGGGACCACA 
     4861 TGGGCCACTT ACTGTATCAG CTGGTGCAGT CAGAAAAACT CAGCAAGCAG GACTTTTTCA 
     4921 AAGGTTTTTC TGAAACCTTG GAGTTGGCAG ATGACATGGC CATTGATATT CCCCATATTT 
     4981 GGTTGTACCT GGCTGAACTG GTCACCCCCA TGTTAAAAGG AGGGGGGATT TCCATGAGAG 
     5041 AACTTATTGT GGAATTCAGC AAGCCATTAC TTCCTGTTGG CAGAGCTGGG GTCCTGCTTT 
     5101 CTGAAATCCT ACACCTTCTA TGCAGACAAA TGAGCCATAA GAAAGTAGGC GCCCTGTGGA 
     5161 GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCGGAAGG GGAAGATGTC CATCATTTTC 
     5221 TCTTGGAGCA GAAGTTGGAC TTCACGGAAT CCGAAGGGCC CTGCTCCTCT GAGGCACTGT 
     5281 CAAAGAAGGA GCTGTCTGCC GAGGAGCTGT CTCAGCGCCT GGAAAAGCTC ATCATGGAGG 
     5341 AGAAAGCGGA TGACGAGCGG ATCTTTGACT GGGTGGAGAC TTGCTCCGGA TGTTTTTTGA 
     5401 TTGCCTGTAT GACGAGGAGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//