LOCUS       MR231800                9168 bp    DNA     circular  SYN 
DEFINITION  Ssh2 (myc-DDK-tagged) - Mouse slingshot homolog 2 (Drosophila) (Ssh2), transcript variant 2
ACCESSION   NM_001291190
VERSION     NM_001291190.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5325..5416 
                     /label="Myc-DDK"  
     misc_feature    5446..6031 
                     /label="PolyA signal"  
     misc_feature    5503..5522 
                     /label="Restriction Enzyme"  
     misc_feature    6307..6979 
                     /label="ColE1"  
     misc_feature    7409..8203 
                     /label="Kan/Neo"  
     misc_feature    8238..8540 
                     /label="SV40 ori"  
     misc_feature    8626..9103 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5307..5313 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5307 
                     /label="Ssh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGAGC ACTTTGGCAG GCGAAAGGAA AGCGCTCCCT GCCAGCACCT 
     1081 GCAGCCTCGG TGGCCCCGAC ATGATCCCCT ACTTCTCTGC CAATGCGGTC ATCTCGCAGA 
     1141 ACGCCATCAA CCAGCTCATC AGCGAGAGCT TCTTAACTGT CAAAGGTGCT GCCCTTTTTC 
     1201 TACCACGAGG GAATGGCTCG TCTACACCAA GAGTCAGCCA CAGACGCAAC AAGCATGCAG 
     1261 GTGATCTCCA ACAGCATCTT CAAGCAATGT TCATATTACT CCGCCCAGAA GACAACATAA 
     1321 GGCTGGCTGT AAGACTGGAA AGTACTTACC AAAACCGAAC ACGCTATATG GTCGTGGTTT 
     1381 CAACTAATGG TAGACAAGAC ACTGAAGAAA GCATTGTCCT GGGAATGGAT TTTTCTTCTA 
     1441 ATGACAGCAC TTGTACCATG GGCTTGGTCT TGCCTCTCTG GAGTGACACC CTAATTCATT 
     1501 TGGATGGTGA TGGTGGGTTC AGCGTATCAA CAGATAACAG AGTTCACATA TTCAAACCTG 
     1561 TATCTGTGCA AGCAATGTGG TCTGCACTAC AGAGTTTGCA CAAGGCTTGT GAAGTGGCCA 
     1621 GAATGCATAA CTACTATCCA GGCAGCCTTT TTCTCACTTG GGTGAGTTAT TACGAGAGCC 
     1681 ATATCAACTC AGATCAGTCC TCAGTCAATG AGTGGAATGC TATGCAGGAT GTGCAGTCTC 
     1741 ATCGGCCTGA CTCTCCAGCC CTCTTCACAG ACATACCAAC TGAACGTGAG CGAACAGAAA 
     1801 GGCTTATTAA GACTAAATTA AGGGAGATCA TGATGCAAAA GGATTTGGAG AATATCACCT 
     1861 CCAAAGAGAT ACGCACGGAG CTGGAGATGC AGATGGTGTG CAACTTGCGA GAATTTAAGG 
     1921 AATTCATAGA TAATGAAATG ATCGTGATCC TTGGTCAGAT GGACAGTCCC ACACAGATAT 
     1981 TTGAGCATGT ATTCCTGGGC TCAGAATGGA ATGCCTCGAA CTTAGAGGAC TTGCAGAACC 
     2041 GAGGGGTACG GTATATCTTG AATGTTACTC GAGAGATAGA TAACTTCTTC CCTGGAGTCT 
     2101 TTGAGTATCA TAACATTCGA GTATATGATG AAGAAGCAAC AGATCTCCTG GCTTACTGGA 
     2161 ATGACACTTA CAAATTCATC TCTAAAGCAA AGAAACATGG ATCTAAATGT CTTGTGCACT 
     2221 GCAAAATGGG GGTGAGTCGC TCAGCCTCCA CTGTGATTGC CTATGCAATG AAGGAGTATG 
     2281 GATGGAATCT GGATCGAGCC TATGACTATG TGAAGGAAAG ACGAACGGTG ACCAAGCCTA 
     2341 ACCCCAGCTT CATGAGACAA CTGGAAGAAT ACCAAGGGAT CTTGCTGGCC AGCAAACAGC 
     2401 GGCATAACAA ACTCTGGAGA TCTCATTCAG ATAGTGACCT TTCAGACCAC CACGAACCCA 
     2461 TCTGTAAACC TGGGCTCGAG CTCAACAAGA AGGAGATGAC CACGTCAGCA GACCAGATCG 
     2521 CTGAGGTGAA GACTGTGGAG AACCTTGCTG CCATGCCTAC CGTCTTTATG GAACATGTGG 
     2581 TCCCACAAGA TGCAAATCAG AAAGGACTAC ACACCAAAGA AAGAGTCATC TGCTTGGAGT 
     2641 TTTCTTCACA AGAGTTTCGT GCCGGACAGA TTGAAGATGA ATTAAATTTA AATGACATCA 
     2701 ATGGATGCTC ATCAGGGTGT TGTCTCAGTG AATCAAAACT CCCTCTTGAC AACTGCCATG 
     2761 CGTCTAAAGC CTTACTCCAA CCTGGACAGG CCCCAGACAT TGCCAACAAG TTCCCAGACT 
     2821 TAGCTGTGGA AGATCTGGAG ACAGACGCAC TGAAAGCAGA CATGAATGTC CACCTACTGC 
     2881 CAATGGAAGA GTTGACATCT CGACTGAAAG ACCTCCCCAT GTCACCTGAT CTGGAGTCAC 
     2941 CAAGCCCCCA AGCCAGTTGC CAAGCTGCCA TCTCAGATTT TAGTACAGAT CGTATTGACT 
     3001 TTTTTAGTGC CCTGGAGAAA TTTGTAGAGC TTTCTCAAGA AACCCGGTCT CGGTCTTTTT 
     3061 CTCACTCAAG GATAGAAGAA TTAGGTGGAG GAAGGAGTGA GGGCTGTCGT TTGTCAGTGA 
     3121 TAGAAGTGGC AGCTTCAGAA ATGGCAGCTG ATGACCAGAG AAGCAGCTCT TTGAGTAATA 
     3181 CGCCCCACGC CTCTGAAGAG TCTTCTGTGG ATGAGGACCA GTCGAAGGCA ATCACCGAAC 
     3241 TTGTCAGCCC AGACATCATC ATGCAATCTC ACTCAGAAAA TGCAATTTCA GTCAAAGAAA 
     3301 TTGTGACTGA AATTGAGTCC ATTAGTCAAG GAGTTGGGCA GGTTCAGTTA AAAGGAGACA 
     3361 TCCTCTCTAA CCCATGCCAC ACACCAAAGA AGAGCACCAT TCATGAGCTG CCCCTGGAGA 
     3421 GGGTGCCAGC CCCTGAGAGC AAACCTGGAC ATTGGGAGCA GGATGAGAGT TTCTGTAGTG 
     3481 TCCAGCCAGA ACTAGCTAGA GACTCAGGGA AGTGTGCCCC GGAGGAAGGC TGTCTGACCA 
     3541 CACACTCATC CACAGCAGAC TTGGAAGAAG AAGAACCAGT TGAGGGGGAA CATGACTGGG 
     3601 GCCCAGGGAT GCACTCTGGT GCTAAGTGGT GCCCTGGGTC TGTGAGGCGA GCCACCCTGG 
     3661 AGTTTGAAGA GCGCTTGCGA CAAGAGCAAG AGAACCATGG TACAGCCTCT GCAGGCCCCA 
     3721 CATTATCCAA TCGCAAAAAC TCTAAGAATG ATTCTTCTGT GGCAGACTTA ATGCCAAAAT 
     3781 GGAAAAGTGA TGAAACTACC CCAGAACATT CATTTTTCCT CAAAGAAGCC GAGCCGAGCA 
     3841 AGGGTAAAGG GAAATGCAGT GGTTCTGAAG CCGGATCACT ATCCCACTGT GAGCGTAATC 
     3901 CCACCATGCC AGACTGTGAG CTGCTGGAGC ATCATTCCTT GCCGGCTCCT CAGGACTGCC 
     3961 TAGGGTCAGA TAGTAGAAGT AAGAAGCAGG AAGGAGACCT AAAGAAGCAG AGGGCTGTGG 
     4021 TTCCAAACCA GGAATGTGAC ACACAGGCCA TCCTTCTCCC CCTTCCCAAG AAAATAGAAA 
     4081 TCATTGAGTA TACGCCGACA GTTACATCAC TGGGTCACAC CGAGCCAGGA GGTGAGGCGA 
     4141 CACCCAGCAA AGAGGGTGAG AAACAAGGAC TGAGGAAAGT GAAGATGGAG CAGTCTATCA 
     4201 CTATGTTTTG TGCGCTGGAT GAAAATCTGA ACAGGACTCT GGAGCCCAGT CAAGTTTCTC 
     4261 TGCATCCCCA AGTGCTACCT CTGCCTCATT CTTCCTCAGA GTGTGACAGG CCCGCTGACC 
     4321 CAAACCCTAT GTTAAGTAGC CCGCAAGACA AAGGGGACTG CCCATCCACA CCCTTCAAGA 
     4381 CAGCAGCACC TTTTGTCAGC TGCAGTACCC AGGGAGCATC TTTCAGCTTG GATTATTTGC 
     4441 TTCCCCACTC TGTGGTTCAC CTGGAAGGCT GCACAGAGCA AAGCAGTGCC ACTGACAATG 
     4501 AACTGTCTCC AGAGCAGGCT AGCTGGGAGG ACAGCCGGGG GCATTTCCTC TCCAGTGGTA 
     4561 GTGGAATGGC ACACACATCC TCCCCATTAA CTAACGAAGA CCTAAGTTTA ATTAACAAAC 
     4621 TTGGTGACAG TGTTGGTGTG TTACAGAAAA AACTGGACCC GTCACCTGAA GCCTGTAGAA 
     4681 TCCCACATAG CTCTAGTAGT GAAAATATAA GAGATCTCAG CCACAGCCGT GGTGTGGTGA 
     4741 AGGAGCATGC TAAAGAAATT GAGTCTCGAG TGATCTTCCA GGCAGGGTTT TCCAAAACAT 
     4801 CCCAAATGAA GCGCTCAGCT TCCCTTGCCA AGTTGGGTTA CCTGGACCTT TGTAAAGACT 
     4861 ACTTACCAGA TAGAGAGCTT GTCTCCTCAG AATCCCCTCA TCTCAAATTG CTTCAGCCCT 
     4921 TCCTCAGAAC AGACTCAGGC ATGCATGCCT TGATGGCCCA TGAGCCCTCA GAAAGCGCAG 
     4981 GTGCCCAGCA GAACCCACAG CCCACCAAGT ATTCTGTAGA GCAACTCAAA ACATCAGAGT 
     5041 GTATTGTGCA GAGCAAACCA GTGGAGAGGC CCAGTGTGCA GTATGCCAAA GAGTTTGGTT 
     5101 ACAGTCAGCA GTGTTTGCTC CCCAAGGCAA GACCAGAATT GACTAGTTCT GAAGGAGGCC 
     5161 TTCCTTTGCT ACAGACACAG GGTCTGCAGT ACACAGGCCC CTCTCCAGGG CTGGCTGTGG 
     5221 CGCCCCGTCA GCAACATGGC AGAACTCACC CCCTTAGAAG ACTGAAAAGA GCAAATGATA 
     5281 AAAAACGGAC AACCAACCCC TTCTATAACA CCATGACGCG TACGCGGCCG CTCGAGCAGA 
     5341 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5401 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5461 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5521 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5581 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5641 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5701 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5761 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5821 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5881 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5941 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6001 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6061 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6121 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6181 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6241 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6301 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6361 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6421 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6481 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6541 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6601 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6661 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6721 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6781 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6841 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6901 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6961 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7021 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7081 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7141 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7201 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7261 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7321 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7381 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7441 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7501 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7561 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7621 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7681 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7741 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7801 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7861 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7921 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7981 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8041 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8101 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8161 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8221 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8281 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8341 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8401 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8461 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8521 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8581 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8641 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8701 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8761 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8821 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8881 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8941 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9001 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9061 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9121 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//