LOCUS       MR231793                9105 bp    DNA     circular  SYN 
DEFINITION  Palld (myc-DDK-tagged) - Mouse palladin, cytoskeletal associated protein (Palld), transcript variant 1
ACCESSION   NM_001293772
VERSION     NM_001293772.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5262..5353 
                     /label="Myc-DDK"  
     misc_feature    5383..5968 
                     /label="PolyA signal"  
     misc_feature    5440..5459 
                     /label="Restriction Enzyme"  
     misc_feature    6244..6916 
                     /label="ColE1"  
     misc_feature    7346..8140 
                     /label="Kan/Neo"  
     misc_feature    8175..8477 
                     /label="SV40 ori"  
     misc_feature    8563..9040 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5244..5250 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5244 
                     /label="Palld"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGAGACT TCCTCCCACG ACTCCTTCTA TGACTCCTTA TCAGACGTGC 
     1081 AGGAAGAGGG CAAAAGTGCT GACTTCTTTC CAGGCCTCTC TGCTTTCCTC AGCCAGGAAG 
     1141 AAATAAATAA AAGCCTTGAC CTGGCACGCA GAGCTATAGA CAGCTCTGAA ACTGAAGATT 
     1201 TTGACTCTGA GAAGGAGATC TCGCAGATTT TCAGCAAGTC TCCCATAAGC CTCTGCGAAA 
     1261 CTCCTTCCCA TGAGGAGCCG AAATCAGGCA AGCAGACTTC CTCAGAACGA CCTCAGGATA 
     1321 GCAGGCGAGC ACCTGTCCAG CCTCTAACAG GAGATCAAGC TGAAAGGATC ACTTCCCCAG 
     1381 GTTCAAAGAG AAAACCCGGG GTATCACCCC TGCTGGCCAG CCCCAGCTAC ATCCGGAGCC 
     1441 TCCGCAAGGC TGAAAAACGA GGTGCAAAAA ATCCCAATCC AAGTTCAAAG CCCAAAACTG 
     1501 CCCAGCAAAG CAAGGCTGGC CCCCAGAGCC AACTGTGCGA CAAGGCGGCT AGTTTCATCG 
     1561 AGGAACTGAC CTCCATATTT AGAGAAGCGG CAAAGCCAAG GAACAGAAGC CCAAATGGTG 
     1621 AGTCCTCATC ACCAGACAGT GGGTATCTGT CTCCTAAAAA TCAGCCATCA GCCCTGATGA 
     1681 GTGCCTCAGC CAGTCAGAGC CCCACTGCAG ACCAGCTAGA TCAACTGGAG ATGGACGCAG 
     1741 AAGTCAAGCA AGCCCAGGGC AGCCTTTGCT ACCAGGCCCA CCAGGCCTCG GAAGAGACCT 
     1801 TGCCACTCGC TCACATCCCG CACCCACAGC CCCAGAAAGC CCGCCACTTG CCAACTGCAC 
     1861 CTAGGTTCAT CCAGAAACTG AGGAGCCAAG AAGTTGCTGA AGGAAGCAGA GTTTATTTGG 
     1921 AGTGTAGAGT CACAGGAAAC CCAACGCCCA GGGTCAGATG GTTCTGTGAG GGGAAGGAGC 
     1981 TGTACAACAG TCCTGACGTT CAGATCCACT GCGAGAGTGG GGAGCTGCAC ACCTTGGTCA 
     2041 TTGCTGAGGC CTTTGAGGAC GACACAGGTC GCTACACGTG TCTGGCTACA AACCCCAGTG 
     2101 GCTCAGACAG CACATCTGCA GAGGTGTTCA TTGAAGGAGC CAGTTCAACG GACTCTGACA 
     2161 GCGAAAGCTT ATCTTTCATA TCAAAAGCTG GAGCCATGCC ACAAGCTCAG AAGAAAACAA 
     2221 CGTCCGTTTC CTTAACGATA GGATCCTCAG CTCCGAAAAC AGGAGTGACC ACAGCTGTGA 
     2281 TTCAGCCCCT ATCTGTGCCC GTCCAACAGG CTCACAGCGC TACTTCATAT CTCTGCCGAC 
     2341 CTGACGGAAC CACCATGGGC TGCCTTCTTC CTGTTTTTAC TAAGGAACTA CAGAACACAG 
     2401 CAGCCTCCGA GGGCCAGGTG GTGGTGCTGG AATGCAGAGT CCGAGGGGCA CCCCCGCTGC 
     2461 AGGTCCAGTG GTTCCGGCAA GGAAGTGAGA TCCAAGACTC TCCGGACTTC AGAATTCTCC 
     2521 AGAAAAAACC CAGATCGACA GCTGAACCCG AGGAGATCTG CACCCTTGTC ATTGCTGAGA 
     2581 GTTTCCCTGA AGATGCAGGC ATCTTTACCT GCTCAGCCAC AAATGACTAC GGCTCAGTGA 
     2641 CCAGCACTGC ACAGCTTGTT ATAACCTCCG CCAACAACGA GAACTGTAGT TACGACTCAA 
     2701 CAGGAGAACC CAACAGTGAT CACTTCCAAC ACTTTCCACC TCCCCCTCCG ATCCTGGAGA 
     2761 CAGGCTCCTA TGAGCTGGCA TCCCAGAAAC CATCTGAAAT CCAGCAGGTG AACAGCCCCA 
     2821 ATTTAGGATT TAGCATGGCA GCTCTTCAAA TGCAATTCAA CACTGCAGAG AGGGAGACCA 
     2881 ACGGAGTCCA TCCCAGCCAT GGAGTCAACG GGCTGATTAA TGGCAAAGCT TATGGCAATA 
     2941 AATCTCCTCC AACACCAACT GCCCTGCTTT CACCCACTAA GGAACCACCG CCACTCCTTG 
     3001 CCAAACCCAA ACTGGATCCT CTGAAGCTTC AGCAGCTGCA GAACCAAGTG CGCCTGGAAC 
     3061 AGGAGGCCTG CGCCTGGCCC CCAGCGCCCC CAGGTGTCCC TTGCAACAGC AGCAGCAGCG 
     3121 GCAGCAGCGC GCCCCCGTCG CCGCCCTTCC CCCCACCGCC ACCAGCCTTT CCGGAGCTCG 
     3181 CGGCCTGTGC GTCGCCGGTG CCCTCGGAGC CCATGAGCGC TCTGGCCTCC CGCGCTACCG 
     3241 CCATGCAGTC CTCCGGCTCC TTCAACTACG CGCGCCCTAA GCAGTTCATC GCGGCGCAGA 
     3301 ACCTGGGTCC CGCATCGGGG CTGCCCACGC CAACCTCCAG CCCCAGCTCC TCCAGCTTGC 
     3361 CGTCACCATT GTCCCCCACG CCCAGGCCCT TCGGCCGCGC GCCCGGGCCG CCCTTCGTGG 
     3421 AACCCGAGGC CATGTGGGGC CCGTCCTCGC CCTCGCCGCC ACCGCCGCCA CCTCCGGTCT 
     3481 TCAGCCCCTC TGCAGCCTAC CCGGTGCCCG ACGTGTTCCC ACTGCCACCG CCGCCGCCAC 
     3541 CGCTACCTAG CTCCACCTCA CACTGCGCCT CGCCCGCCCG CTTCGGCCCC AGCCAGACGC 
     3601 CCGCAGCCTT CCTCAGCGCC CTGCTGCCCT CTCAGCCACC GCCTGTCGCT GTCAACGCCC 
     3661 TGGGGCTGCC CAAGGGAGTC ACCCCCGCGG GGTTCCCAAA GAAGTCCAGT AGAACTGCTA 
     3721 GAATTGCCTC TGATGAGGAG ATTCAAGGCA CAAAGGATGC TGTCATCCAA GACCTGGAAC 
     3781 GGAAGCTTCG CTTCAAGGAG GACCTTCTGA ACAATGGCCA ACCGAGGCTA ACCTATGAGG 
     3841 AAAGAATGGC TCGCCGCCTG CTTGGAGCCG ACAGCGCAAA CGTCTTCAAC ATCCAGGAGC 
     3901 CAGAGGAAAC GGCAGCCAAT CAGGACGCTG GGGCTCCTCG GGCTTCTGTA GGGGGTCCTC 
     3961 TGGATGGTCA AAAGGAGTAC AAAGTCTCTA GCTGCGAGCA GAGGCTGATT AGCGAGATTG 
     4021 AGTACCGGCT GGAGCGCTCC CCTGTGGATG AGTCGGGAGA CGAGGTGCAG GATCCAGATG 
     4081 TGCCTGTGGA GAACGCAACA GCTCCCTTCT TTGAGATGAA GCTGAAACAC TACAAGATCT 
     4141 TTGAGGGGAT GCCGGTGACT TTCACGTGTC GAGTGGCTGG GAATCCAAAG CCAAAGATCT 
     4201 ATTGGTTTAA AGATGGGAAG CAGATTTCTC CGAAGAGCGA TCACTACACC ATTCAGAGAG 
     4261 ACCTTGATGG GACCTGCTCT CTCCACACCA CGGCCTCTAC CCTAGACGAC GATGGGAACT 
     4321 ACACCATCAT GGCTGCCAAC CCTCAGGGTC GCGTCAGTTG TACAGGAAGG CTAATGGTAC 
     4381 AGGCTGTCAA CCAAAGAGGC CGCAGTCCCC GCTCTCCCTC AGGCCATCCT CATGCCAGAA 
     4441 GGCCTCGCTC TCGATCACGG GACAGTGGAG ATGAAAACGA GCCCATTCAG GAGCGATTCT 
     4501 TCAGACCTCA CTTCCTGCAG GCTCCTGGAG ACCTGACCGT TCAGGAAGGC AAGCTCTGCA 
     4561 GGATGGACTG CAAGGTCAGT GGGTTACCAA CCCCAGATCT CAGCTGGCAA CTAGATGGAA 
     4621 AGCCCATACG CCCCGACAGT GCTCACAAGA TGCTGGTCCG TGAGAATGGG GTCCACTCCC 
     4681 TCATTATAGA GCCAGTCACG TCCCGGGACG CCGGCATCTA CACATGTATT GCCACCAACA 
     4741 GAGCAGGACA GAACTCGTTT AACCTGGAGC TTGTGGTTGC TGCTAAGGAA GCACACAAGG 
     4801 CCCCTGTGTT TATGGAGAAG CTACAGAACA CGGGGGTTGC TGATGGATAC CCAGTGCGGC 
     4861 TGGAATGCCG TGTCTCGGGA GTGCCGCCAC CTCAGATATT TTGGAAGAAA GAAAATGAAT 
     4921 CGCTCACTCA CAGCACTGAG CGAGTAAGCA TGCACCAGGA TAATCATGGC TACATCTGCC 
     4981 TGCTCATCCA GGGAGCCACA AAGGAAGACG CTGGGTGGTA CACTGTGTCC GCCAAGAACG 
     5041 AAGCAGGCAT TGTGTCCTGC ACTGCCCGGC TGGATGTCTA CACCCAGTGG CACCAGCAGC 
     5101 CACAGACCAC CAAGCCAAAA AAAGTACGGC CCTCGGCCAG TCGCTACGCA GCACTTTCGG 
     5161 ACCAGGGACT AGACATCAAA GCCGCTTTCC AACCTGAAGC CAGCCCATCT CACCTGACTC 
     5221 TGAACAGCGG CTTGGTAGAA AGTGAAGACC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5281 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5341 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5401 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5461 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5521 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5581 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5641 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5701 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5761 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5821 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5881 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5941 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6001 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6061 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6901 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6961 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7021 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7081 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7141 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7201 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7261 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7321 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7381 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7441 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7501 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7561 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7621 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7681 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7741 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7801 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7861 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7921 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7981 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8041 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8101 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8161 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8221 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8281 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8341 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8461 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8521 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8581 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8641 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8701 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8761 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8821 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8881 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8941 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9001 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9061 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//