LOCUS       MR231792                9099 bp    DNA     circular  SYN 
DEFINITION  Edc4 (myc-DDK-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4), transcript variant 1
ACCESSION   NM_001301100
VERSION     NM_001301100.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5256..5347 
                     /label="Myc-DDK"  
     misc_feature    5377..5962 
                     /label="PolyA signal"  
     misc_feature    5434..5453 
                     /label="Restriction Enzyme"  
     misc_feature    6238..6910 
                     /label="ColE1"  
     misc_feature    7340..8134 
                     /label="Kan/Neo"  
     misc_feature    8169..8471 
                     /label="SV40 ori"  
     misc_feature    8557..9034 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5238..5244 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5238 
                     /label="Edc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCCTGC GCGAGCATCG ACATTGAGGA CGCCACGCAG CATCTGCGGG 
     1081 ACATACTCAA ACTGGACCGG CCCGCCGGAG GTTCCAATGC CGAAAGCCAA AGGCCATCCA 
     1141 GTGCCTACAA TGGAGACCTC AATGGGCTCC TGGTCCCCGA CCCGCTCAGT TCAGGTGATG 
     1201 GTAACTCAAC GAACAAACCT GGTATACGGA CCATGCCACC CATTAACCTG CAGGAAAAGC 
     1261 AGGTCATTTG CCTCTCTGGA GATGACAGCT CCACCTGCAT TGGGATTTTA GCCAAGGAAG 
     1321 TGGAAATTGT GGCCAGCAGT GATTCTAGCA TTTCCAGCAA GGCTCGGGGG AGCAACAAGG 
     1381 TGAAAATCCA GCCTGTCGCC AAGTATGACT GGGAGCAGAA ATACTACTAT GGCAACCTGA 
     1441 TTGCAGTGTC TAACTCCTTC TTGGCATACG CCATTAGGGC TGCCAACAAT GGCTCAGCAA 
     1501 TGGTCCGTGT GATCAGTGTC AGCACTTCGG AGCGGACCCT GCTCAAAGGT TTCACAGGCA 
     1561 GTGTGGCTGA TCTTGCTTTT GCACACCTCA ACTCTCCACA GCTCGCCTGC CTGGATGAGG 
     1621 CTGGCAATCT TTTTGTGTGG CGCTTGGCTC TTGTTAAGGG TAAAATTCAA GAAGAGATTT 
     1681 TAGTCCATAT CCGGCAGCCA GAGGGTACAG CGCTGAACCA CTTCCGAAGG ATCATCTGGT 
     1741 GCCCTTTCAT CCCCGAGGAG AGTGAGGACT GTTGTGAGGA GAGCAGCCCG ACAGTGGCCC 
     1801 TGCTGCATGA GGATCGGGCT GAGGTGTGGG ACTTGGATAT GCTTCGCTCC AGCCACAACA 
     1861 CCTGGCCTGT GGATGTCAGC CAAATCAAGC AAGGCTTTAT TGTGGTGAAA GGCCATAGCA 
     1921 CGTGCCTAAG TGAAGGAGCC CTCTCCCCTG ATGGGACTGT CCTGGCTACC GCAAGCCATG 
     1981 ATGGCTTTGT CAAGTTCTGG CAGATCTATA TTGAAGGTCA GGATGAGCCA AGGTGTCTGC 
     2041 ATGAATGGAA GCCTCATGAT GGGAGGCCTC TTTCTTGCCT CCTGTTCTGT GATAACCATA 
     2101 AGAAACAGGA TCCTGAGGTC CCGTTCTGGA GGTTCCTCAT AACCGGCGCC GACCAGAATC 
     2161 GAGAGCTGAA GATGTGGTGC ACAGTGTCCT GGACCTGCCT GCAAACCATT CGCTTCTCCC 
     2221 CAGATATCTT CAGCTCAGTG AGTGTGCCTC CCAGCCTCAA AGTTTGTTTG GACCTCTCAG 
     2281 CAGAGTATTT GATTCTTAGT GATGTGCAAC GAAAGGTCCT CTATGTGATG GAGCTGCTGC 
     2341 AGAACCAGGA CGAGGGCCGC GCGTGCTTTA GTTCTATCTC TGAGTTCCTG CTCACCCACC 
     2401 CTGTGCTGAG CTTTGGCATC CAGGTTGTCA GTCGCTGCCG GCTACGGCAC ACTGAGGTCC 
     2461 TGCCTGCTGA GGAGGAGAAT GACAGTCTGG GGACCGAGAG TTCCCATGGA GCTGGAGCCT 
     2521 TGGAATCCGC AGCTGGTGTG CTTATCAAGC TCTTTTGTGT GCACACTAAG GCACTACAAG 
     2581 ATGTGCAGAT CCGCTTCCAG CCACAGCTGA ACCCTGATGT GGTGGCCCCA CTCTCTACCC 
     2641 ACACTGCCCA CGAGGACTTC ACATTTGGAG AGTCTCGACC TGAACTAGGC TCCGAGGGGC 
     2701 TAGCTTCAGC TGCTCATGGT TCCCAGCCTG ACCTGCGGCG CATTGTGGAA CTGCCTGCTC 
     2761 CTGCAGACTT CCTCAGTCTG AGCAGTGAGA CCAAGCCCAA GTTGATGACA CCTGATGCTT 
     2821 TCATGACACC TACTGCCTCC CTGCAGCAGA TCTCTGCATC CCCTAGTAGT AGCAGCAGCA 
     2881 GTAGCAGCAG CAGCAGTAGC AGCAGCAGCA GCAGCAGCAG CTCTCTAACA GCTGTGTCTG 
     2941 CTGTGAGCAG CTCCTCAGCC ATGGACCCTT CCTTGCCCAG ACCACCTGAG GAGCTGACCT 
     3001 TGAGCCCCAA GCTGCAGCTA GATGGCAGCC TGACGCTAAA CAGCAGCAGC AGCAGCCTGC 
     3061 AGGCAAGCCC TCGAAGCCTC CTTCCGGGCC TGCTCCCAGG CCCAGCTGAC AAGTTGATTT 
     3121 CCAAGGGACC TGGTCAGGTA TCCACTGCTG CCTCTGCACT GTCCTTGGAT TTGCAGGAAG 
     3181 TGGAGCCATT AGGGCTACCC CAGGCCTCCC CAAGTCGAAC CCGTTCTCCT GATGTGATCT 
     3241 CCTCAGCATC CACTGCCCTG TCCCAGGACA TTCCTGAAAT CGCATCTGAG GCCCTGTCCC 
     3301 GTGGCTTTGG CTCCTCTGTT CCTGAGGGCC TCATTGAGCC AGACAGCATG GCCTCAGCTG 
     3361 CCTCAGCACT ACACCTACTC TCTCCTCGGC CCAGGCAAGG CCCTGAGCTT GGCTCTCAGC 
     3421 TGGGTCTGGA TGGAGGCCCT GGAGATGGGG ATAGGCATAG TACCCCTTCC CTGCTGGAAG 
     3481 CAGCCCTGAC CCAGGAAGTT GCAACCCCTG ACAGTCAAGT CTGGCCTACG GCACCTGACA 
     3541 TTACTCGTGA GACCTGTAGC ACACTGACAG AAAGCCCCAG GAATGGCCTC CAGGAAAAGC 
     3601 ACAAAAGTCT GGCTTTCCAC AGGCCACCTT ATCACTTGCT GCAGCAACGT GACAGTCAGG 
     3661 ACACAAGTGC TGAGCAAAGT GACCATGATG ATGAGGTTGC CAGCCTTGCC TCTGCCTCAG 
     3721 GAGGTTTTGG CAGCAAAATT CCTACTCCAC GGCTGCCTTC CAAGGATTGG AAGACGAAGG 
     3781 GATCCCCTCG GACGTCACCT AAGCTCAAGA GAAAAAGCAA GAAGGATGAT GGGGATTCAG 
     3841 CTGTGGGATC CCGGCTCACA GAGCACCAGG TGGCAGAGCC CCCTGAAGAC TGGCCAGCAC 
     3901 TAATTTGGCA GCAGCAAAGA GAGCTGGCAG AGCTATGGCA CAACCAAGAA GAACTGCTAC 
     3961 AGCGTCTCTG TGCCCAGCTT GAAGGTCTTC AGAGTACTGT CACAGACCAT GTAGAGCGTG 
     4021 CCCTGGAGAC GCGGCATGAG CAGGAGCAAC GGCGGCTGGA ACGGGCACTG GCTGAGGGGC 
     4081 AGCAACGGGG TGGGCAGCTG CAGGAGCAGC TGACACAGCA GCTATCCCAG GCATTGTCTT 
     4141 CAGCTGTGGC TGGGCGGCTA GAGCGCAGCG TAAGGGATGA AATCAAGAAA ACGGTTCCTC 
     4201 CATGTGTCTC CAGAAGTCTG GAGCCTGTTG CAGGTCAACT AAGCAACTCA GTGGCTACCA 
     4261 AGCTTACAGC TGTGGAAGGC AGCATGAAAG AGAATATCTC TAAGCTACTC AAGTCCAAGA 
     4321 ACTTAACAGA TGCCATTGCC CGAGCAGCTG CAGACACGTT ACAGGGACCA ATGCAGGCTG 
     4381 CCTACCGGGA AGCCTTCCAG AGTGTGGTAC TGCCAGCTTT TGAGAAGAGT TGCCAGGCTA 
     4441 TGTTCCAGCA AATCAATGAC AGCTTTAGGC TGGGCACACA GGAATATTTG CAGCAGCTGG 
     4501 AGAGTCACAT GAAGAGCCGG AAGGCCCGTG AACAAGAAGC TAGGGAGCCT GTGCTGGCCC 
     4561 AGCTTCGGGG CCTGGTCAGC ACACTGCAGA GTGCCACCGA GCAGATGGCG GCCACTGTAT 
     4621 CTAGCAGTGT TCGGGCCGAG GTGCAACACC AGCTGCATGT GGCTGTGGGC AGCTTGCAGG 
     4681 AGTCAATCTT AGCACAAGTA CAGCGCATTG TCAAGGGTGA AGTGAGTGTG GCACTTAAGG 
     4741 AGCAGCAGGC CACCGTCACT TCCAGCATCA TGCAGGCTAT GCGTTCAGCT GCTGGCACAC 
     4801 CTGTCCCCTC TGCCCACCTT GACTGCCAGG CCCAACAAGC CCATATCTTG CAGTTACTGC 
     4861 AGCAGGGCCA CCTCAATCAG GCCTTCCAGC AGGCACTGAC TGCTGCTGAT CTCAATCTGG 
     4921 TGCTGTATGT GTGTGAAACT GTCGACCCAG CCCAGGTTTT TGGGCAGCCG CCCTGTCCAC 
     4981 TATCCCAGCC TGTTCTCCTT TCCCTAATCC AGCAGCTAGC ATCTGACCTT GGAACTAGAA 
     5041 GTGACCTCAA GCTCAGCTAC TTGGAAGAAG CTGTGATGCA CCTGGACCAC AGCGATCCCA 
     5101 TCACTCGAGA CCACATGGGC TCTGTCATGG CTCAGGTGCG CCAGAAGCTC TTCCAGTTCC 
     5161 TGCAGGCTGA TCCACACAAC TCACTTAGCA AAGCTGCCCG CCGCCTCAGC CTAATGTTAC 
     5221 ACGGCCTTGT GACCCCTAGC CTCCCTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5281 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5341 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5401 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5461 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5521 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5581 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5641 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5701 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5761 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5821 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5881 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5941 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6001 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6061 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6121 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6181 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6241 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6301 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6361 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6421 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6481 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6541 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6601 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6661 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6721 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6781 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6841 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6901 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6961 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7021 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7081 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7141 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7201 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7261 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7321 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7381 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7441 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7501 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7561 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7621 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7681 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7741 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7801 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7861 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7921 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7981 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8041 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8101 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8161 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8221 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8281 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8341 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8401 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8521 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8581 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8641 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8701 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8761 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8821 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8881 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8941 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9001 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9061 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//