LOCUS       MR231790                9084 bp    DNA     circular  SYN 
DEFINITION  Brd4 (myc-DDK-tagged) - Mouse bromodomain containing 4 (Brd4), transcript variant 3
ACCESSION   NM_001286630
VERSION     NM_001286630.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5241..5332 
                     /label="Myc-DDK"  
     misc_feature    5362..5947 
                     /label="PolyA signal"  
     misc_feature    5419..5438 
                     /label="Restriction Enzyme"  
     misc_feature    6223..6895 
                     /label="ColE1"  
     misc_feature    7325..8119 
                     /label="Kan/Neo"  
     misc_feature    8154..8456 
                     /label="SV40 ori"  
     misc_feature    8542..9019 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5223..5229 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5223 
                     /label="Brd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTACGGAG AGCGGCCCTG GGACAAGATT GAGAAATCTG CCAGTAATGG 
     1081 GGGATGGACT AGAAACCTCC CAAATGTCTA CAACGCAGGC CCAGGCCCAA CCCCAGCCAG 
     1141 CAAATGCAGC CAGCACCAAT CCTCCACCCC CAGAGACCTC CAACCCTAAC AAGCCCAAGA 
     1201 GACAGACAAA CCAACTGCAA TATCTGCTCA GAGTGGTGCT CAAGACACTA TGGAAACACC 
     1261 AGTTTGCGTG GCCTTTCCAG CAGCCCGTGG ATGCCGTCAA GCTGAACCTC CCTGATTACT 
     1321 ATAAGATTAT TAAAACACCC ATGGATATGG GAACAATAAA GAAGCGCTTG GAAAACAACT 
     1381 ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA AATTGTTACA 
     1441 TCTATAACAA GCCTGGAGAT GACATCGTCT TAATGGCAGA AGCTCTGGAG AAGCTCTTCT 
     1501 TGCAAAAAAT CAATGAACTG CCTACAGAAG AAACTGAGAT CATGATAGTC CAGGCAAAAG 
     1561 GAAGAGGACG AGGGAGGAAA GAAACAGGTA GGGCAGCAAA GCCTGGTGTA TCCACGGTAC 
     1621 CAAACACAAC TCAAGCATCA ACTTCTCCGC AGACCCAGAC GCCTCAGCAG AACCCTCCTC 
     1681 CACCTGTGCA GGCCACAACT CACCCCTTTC CTGCTGTCAC CCCAGACCTC ATTGCCCAGC 
     1741 CTCCTGTCAT GACAATGGTG CCCCCTCAGC CACTTCAGAC TCCTTCACCG GTACCCCCCC 
     1801 AGCCACCACC CCCACCTGCT CCAGTTCCAC AGCCTGTGCA GAGTCACCCG CCCATCATTG 
     1861 CGACCACCCC CCAGCCTGTG AAGACAAAGA AAGGGGTGAA GAGGAAAGCA GATACCACCA 
     1921 CCCCTACCAC CATCGACCCC ATTCATGAGC CACCCTCACT GGCCCCAGAG CCCAAGACCG 
     1981 CCAAGCTGGG TCCTCGGCGG GAGAGCAGCA GACCTGTGAA GCCTCCAAAG AAGGATGTAC 
     2041 CGGACTCACA GCAGCACCCA GGGCCAGAGA AGAGCAGCAA GATCTCTGAG CAGCTAAAGT 
     2101 GCTGCAGTGG CATCCTCAAG GAGATGTTTG CCAAGAAACA TGCTGCCTAT GCCTGGCCTT 
     2161 TCTACAAGCC TGTGGATGTG GAGGCACTGG GTCTGCACGA CTACTGTGAC ATCATCAAAC 
     2221 ATCCCATGGA CATGAGCACA ATCAAGTCTA AACTAGAGTC CCGAGAGTAC AGAGATGCCC 
     2281 AGGAATTTGG TGCTGATGTC CGATTGATGT TCTCCAACTG CTACAAGTAC AACCCCCCTG 
     2341 ACCATGAAGT GGTAGCCATG GCTCGAAAAC TCCAGGATGT GTTTGAAATG CGCTTTGCCA 
     2401 AGATGCCTGA TGAGCCTGAA GAGCCAGTTG TTACAGTGTC CTCTCCTGCA GTGCCACCCC 
     2461 CTACAAAGGT GGTAGCCCCA CCCTCATCTA GTGACAGCAG CAGCGACAGT TCTTCCGACA 
     2521 GCGACAGTTC CACTGACGAC TCTGAGGAAG AGCGAGCCCA GCGGCTGGCT GAACTCCAGG 
     2581 AACAGCTCAA GGCCGTGCAT GAGCAGCTTG CAGCCCTCTC ACAGCCCCAG CAGAACAAAC 
     2641 CAAAGAAAAA GGAGAAGGAC AAGAAGGAAA AGAAAAAGGA AAAGCACAAA AAGAAAGAAG 
     2701 AAGTGGAGGA AAATAAAAAA AGCAAAACCA AGGAACTTCC TCCCAAAAAG ACAAAGAAAA 
     2761 ATAACAGCAG CAACAGCAAT GTGAGCAAGA AGGAACCAGT ACCCACGAAG ACCAAGCCGC 
     2821 CTCCCACATA TGAATCAGAA GAGGAGGATA AGTGTAAGCC CATGTCTTAT GAGGAGAAGC 
     2881 GGCAGCTAAG TCTAGATATC AACAAACTTC CTGGTGAGAA GCTAGGCCGT GTAGTACACA 
     2941 TAATTCAGTC AAGGGAACCA TCACTTAAAA ACTCCAACCC CGATGAGATT GAGATTGACT 
     3001 TTGAGACCCT GAAGCCATCT ACACTACGAG AGTTGGAGCG ATATGTCACC TCCTGTTTGC 
     3061 GGAAGAAAAG GAAACCTCAA GCTGAAAAAG TTGACGTGAT TGCTGGTTCT TCCAAGATGA 
     3121 AGGGATTCTC ATCCTCTGAG TCGGAGAGCA CCAGCGAATC CAGCTCCTCT GACAGTGAAG 
     3181 ACTCTGAAAC AGAGATGGCT CCCAAGTCAA AAAAGAAGGG GCACACTGGG AGGGACCAGA 
     3241 AAAAGCATCA TCACCATCAC CATCCACAGA TGCAGCCAGC CCCAGCTCCT GTGCCCCAGC 
     3301 AGCCGCCCCC ACCTCCACAG CAGCCTCCTC CACCCCCACC TCCGCAGCAG CAGCAGCAGC 
     3361 AACCTCCACC CCCACCACCT CCGCCCTCCA TGCCACAGCA GACTGCCCCA GCGATGAAGT 
     3421 CCTCGCCCCC ACCCTTCATC ACTGCCCAGG TCCCCGTCCT GGAACCACAG CTGCCAGGCA 
     3481 GTGTCTTTGA CCCTATTGGC CACTTCACCC AGCCCATCTT GCACCTGCCG CAGCCGGAGC 
     3541 TGCCTCCTCA CCTGCCCCAG CCACCTGAGC ACAGCACTCC ACCCCATCTC AACCAGCATG 
     3601 CTGTGGTCTC TCCTCCAGCT TTGCACAATG CGCTGCCCCA ACAGCCATCT CGGCCCAGTA 
     3661 ACCGAGCTGC TGCTCTGCCC CCAAAGCCTA CCCGACCCCC AGCTGTGTCC CCTGCCCTGG 
     3721 CCCAGCCCCC CCTGCTCCCA CAACCACCAA TGGCTCAGCC CCCCCAAGTG CTGCTGGAGG 
     3781 ATGAAGAGCC ACCTGCCCCA CCCCTCACCT CCATGCAGAT GCAGTTGTAC CTACAGCAGC 
     3841 TGCAGAAGGT GCAGCCTCCC ACACCACTAC TCCCTTCCGT GAAGGTGCAG TCCCAACCCC 
     3901 CACCGCCTTT GCCGCCTCCA CCCCACCCTT CTGTGCAGCA GCAGCAGCTC CAGCCACAGC 
     3961 CACCGCCACC CCCACCTCCT CAGCCACAGC CACCACCCCA GCAACAACAC CAGCCTCCAC 
     4021 CACGACCAGT TCACTTGCCA TCCATGCCCT TTTCTGCTCA TATTCAGCAG CCCCCACCAC 
     4081 CCCCAGGACA GCAACCTACT CACCCACCCC CAGGACAGCA GCCCCCACCA CCACAGCCTG 
     4141 CCAAGCCCCA GCAAGTCATC CAGCATCACC CTTCCCCCCG GCACCACAAG TCAGACCCCT 
     4201 ACTCAGCTGG TCATCTTCGT GAGGCTCCCT CTCCGCTTAT GATACATTCC CCTCAGATGC 
     4261 CACAGTTCCA GAGCCTGACC CATCAGTCTC CTCCCCAGCA AAACGTCCAG CCTAAGAAGC 
     4321 AGGTAAAGGG CAGGGCTGAG CCACAGCCAC CAGGGCCAGT CATGGGCCAA GGCCAGGGAT 
     4381 GCCCACCTGC CTCACCGGCT GCCGTGCCTA TGCTGTCCCA GGAGCTGCGA CCACCTTCAG 
     4441 TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA CCAATCATTC 
     4501 GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA GAGAACATCA 
     4561 AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA GGGAGGCCTG 
     4621 TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG GACAAACAGA 
     4681 AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG AACATGGGCT 
     4741 CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC AAGTCCTCAA 
     4801 GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG GAAAAGGCCC 
     4861 TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG GAGCGCATGA 
     4921 GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG GAGGCACGAC 
     4981 GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG CAGCAGCAGC 
     5041 AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC CAGCCCCAGT 
     5101 CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG AGGCGCAGGG 
     5161 AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA ATATTTGAAG 
     5221 AAAATCTTTT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5281 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5341 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5401 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5461 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5521 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5581 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5641 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5701 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5761 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5821 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5881 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5941 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6001 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6061 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6121 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6181 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6241 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6301 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6361 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6421 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6481 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6541 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6601 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6661 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6721 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6781 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6841 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6901 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6961 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7021 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7081 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7141 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7201 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7261 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7321 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7381 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7441 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7501 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7561 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7621 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7681 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7741 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7801 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7861 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7921 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7981 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8041 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8101 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8161 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8221 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8281 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8341 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8401 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8461 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8521 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8581 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8641 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8701 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8761 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8821 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8881 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8941 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9001 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9061 TAACAAAAAT TTAACGCGAA TTTT
//