LOCUS       MR231770                8844 bp    DNA     circular  SYN 
DEFINITION  Spag9 (myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 5
ACCESSION   NM_001199203
VERSION     NM_001199203.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAG GACGGTGTGG TATACCAGGA GGAGCCCGGA GGCTCCGGGG 
     1081 CCGTGATGTC AGAGCGGGTG TCCGGCCTGG CCGGCTCCAT CTACCGCGAG TTCGAGCGGC 
     1141 TCATCGGGCG CTACGATGAG GAGGTGGTCA AGGAGCTGAT GCCACTGGTG GTGGCCGTGC 
     1201 TGGAGAACCT GGACTCGGTG TTCGCGCAGG ACCAGGAGCA CCAGGTGGAG CTGGAGCTGC 
     1261 TGCGGGACGA CAACGAGCAG CTCATCACCC AGTACGAGCG GGAGAAGGCG CTGCGCAAGC 
     1321 ACGCCGAGGA GAAATTCATT GAATTTGAAG ACTCTCAAGA ACAGGAAAAA AAGGATTTAC 
     1381 AGACGCGAGT GGAGTCTCTA GAATCTCAAA CAAGACAACT TGAACTCAAA GCAAAAAACT 
     1441 ATGCTGACCA GATCAGCAGA CTTGAAGAGA GAGAAGCAGA ACTGAAGAAG GAATATAATG 
     1501 CACTGCATCA GAGACACACT GAGATGATCC ATAATTATAT GGAACACTTA GAAAGAACCA 
     1561 AACTTCATCA GCTCTCAGGG AGTGATCAAC TAGAGGCTAC AGCTCATAGT AGAATTAGAA 
     1621 AAGAACGTCC TATATCATTA GGGATTTTCC CTCTACCTGC TGGAGATGGA TTGCTTACAC 
     1681 CTGACACTCA GAAAGGAGGC GAGACCCCAG GATCAGAGCA ATGGAAATTT CAAGAATTAA 
     1741 GTCAACCACG TTCTCATACC AGCCTGAAGG TCAGCCATAG CCCTGAACCT CTGAAGGCTG 
     1801 TAGAGCAGGA GGATGAACTT TCTGATATTA GTCAAGGTGG ATCTAAAGCT ACCACTCCAG 
     1861 CTTCAACAGC AAATTCAGAC GTATCAGCAA TTCCTCCTGA TACTCCGTCA AAGGAAGATA 
     1921 ATGAAGGATT TGTAAAAGGC ACAGATACAT CAAATAAGTC AGAGATAAGC AAACACATAG 
     1981 AAGTCCAGGT TGCCCAAGAG ACTAGGAATG TGTCTACAGA ATCTGGTGAA AATGAAGAGA 
     2041 AATCAGAAGT TCAAGCAATC ATTGAATCCA CTCCTGAATT GGATATGGAC AAAGACCTCA 
     2101 GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT TTTGATCGCA 
     2161 ATACAGAATC TCTCTTTGAA GAGCTGTCCT CAGCTGGCTC GGGCCTTATT GGAGATGTGG 
     2221 ATGAAGGAGC GGATTTACTA GGAATGGGTC GTGAGGTGGA GAATCTCATA CTGGAGAACA 
     2281 CACAGCTGCT GGAAACCAAA AACGCTCTGA ATGTAGTGAA GAATGACTTA ATAGCCAAAG 
     2341 TGGATGAGCT GACCTGTGAG AAGGACGTGC TGCAGGGGGA GCTGGAGGCT GTGAAGCAAG 
     2401 CCAAGCTGAA GCTGGAGGAC AAGAACAGAG AGCTGGAGGA GGAGCTCAGG AAAGCTCGTG 
     2461 CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGACGATGA TGATAGTGAC ATTCCCACAG 
     2521 CCCAGAGGAA GAGGTTTACC AGAGTAGAAA TGGCCCGAGT TCTCATGGAG AGAAACCAGT 
     2581 ACAAAGAGAG GCTGATGGAG CTTCAAGAAG CTGTTCGATG GACGGAGATG ATCCGGGCCT 
     2641 CACGAGAGAA CCCAGCTATG CAAGAAAAGA AAAGGTCAAG CATCTGGCAA TTTTTCAGCC 
     2701 GACTTTTTAG CTCTTCAAGT AATGCAACAA AGAAGCCTGA ACCCCCTGTT AACCTGAAGT 
     2761 ACAACGCCCC CACCTCTCAC GTGACTCCCT CCGTGAAGAA AAGGAGCAGC ACTTTATCTC 
     2821 AGCTCCCTGG GGACAAGTCC AAGGCATTTG ATTTCCTCAG TGAGGAAACT GAGGCTAGTT 
     2881 TAGCCTCCCG CAGAGAGCAA AAGAGAGAGC AGTATCGGCA GGTGAAGGCT CACGTTCAGA 
     2941 AGGAAGATGG CCGAGTGCAA GCTTTCGGCT GGAGTCTGCC TCAGAAGTAC AAACAGGTAG 
     3001 CCAATGGTCA AGGGGAAACT AAGATGAAAA ATTTACCTGT GCCTGTCTAT CTCAGACCAC 
     3061 TAGACGAAAA AGATGCGTCT ATGAAGCTGT GGTGTGCTGT AGGAGTCAAT TTATCTGGTG 
     3121 GCAAGACAAG AGATGGCGGG TCTGTTGTTG GAGCGAGTGT ATTTTACAAG GATATTGCTG 
     3181 GTTTGGACAC TGAAGGCAGT AAACAGCGGA GTGCGTCTCA GAGTAGTTTA GACAAGTTGG 
     3241 ATCAAGAACT CAAGGAACAG CAGAAAGAAT TTAAAAATCA AGAAGAGTTG TCCAGTCAGG 
     3301 TCTGGATCTG TACCAGCACC CACTCAACTA CAAAGGTTAT CATCATTGAT GCTGTTCAGC 
     3361 CTGGCAACAT CCTAGATAGT TTCACTGTTT GCAATTCTCA TGTTCTGTGC ATTGCCAGTG 
     3421 TCCCAGGAGC TCGGGAAACA GACTACCCTG CAGGAGAAGA ACTTTCTGAA TCTGGTCAGG 
     3481 TAGACAAAGC GTCATTGTGT GGAAGCATGA CCAGTAACAG CTCCGCAGAG ATGGACAGCT 
     3541 TGCTGGGAGG CATCACAGTG GTTGGGTGCT CCACAGAAGG ACTGACAGGA GCTGCCACTT 
     3601 CCCCAAGTAC CAACGGTGCT TCTCCTGTGA TTGAAAAGCC ACCAGAAATG GAAACTGAAA 
     3661 ATAGCGAAGT TGATGAAAAT ATTCCAACAG CAGAAGAAGC AACTGAAGCC ACAGAGGGCA 
     3721 ATGCAGGGTC CACTGAAGAC ACTGTGGACA TCTCCCAGCC TGGCGTGTAC ACAGAGCATG 
     3781 TGTTTACAGA TCCACTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG TTTCAGTCAA 
     3841 GTAATGACTC AGATGTGTAC AAAGATCAAA TATCAGTATT GCCAAATGAA CAAGACCTTG 
     3901 CGAGAGAAGA AGCTCAGAAA ATGAGTAGTC TTTTACCAAC CATGTGGCTT GGAGCTCAGA 
     3961 ATGGCTGTTT GTATGTCCAC TCATCTGTAG CCCAGTGGAG GAAATGTCTC CATTCCATTA 
     4021 AGCTCAAAGA CTCAATTCTC AGTATTGTAC ATGTGAAAGG AATTGTACTA GTGGCCCTGG 
     4081 CGGATGGCAC CCTTGCAATC TTCCACAGAG GAGTTGATGG ACAATGGGAT CTGTCAAACT 
     4141 ATCACCTTTT AGACCTTGGA CGTCCTCATC ATTCCATACG ATGCATGACT GTGGTACATG 
     4201 ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT TCAACCAAAG GCTATGAAGA 
     4261 TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTACGGCAG CTTGCATGGG 
     4321 TGGGTGATGG TGTATGGGTC TCCATTCGTT TGGATTCCAC GCTCCGCCTC TATCATGCAC 
     4381 ACACATACCA ACATCTCCAG GATGTGGATA TTGAGCCTTA TGTAAGCAAA ATGTTAGGTA 
     4441 CTGGAAAACT GGGTTTCTCT TTCGTGAGGA TAACAGCTCT TATGGTGTCT TGCAATCGTT 
     4501 TGTGGGTGGG GACTGGAAAT GGTGTCATTA TCTCCATCCC ACTGACAGAA ACAAATAAAA 
     4561 CCTCAGGGAC TCCAGGTAAC CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAGAACA 
     4621 GTGATAAAGT GACTCCAGGG ACATTTATAC CCTACTGTTC AATGGCACAT GCACAGCTTT 
     4681 GTTTCCACGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAGGTCATTA 
     4741 GCCCACAAAG TAGCAGTGGT GGGGCAGATC TTACAGCTGA CAAGGCAGGG TCATCTGCAC 
     4801 AGGAGCCCAG TAGCCAGACG CCCTTGAAGT CCATGCTTGT CATCAGTGGA GGAGAGGGCT 
     4861 ACATTGACTT CCGGATGGGT GATGAAGGTG GAGAATCTGA ACTTCTGGGA GAGGATCTTC 
     4921 CACTGGAACC TTCAGTCACC AAAGCCGAGA GGAGTCACCT GATAGTGTGG CAAGTGATGT 
     4981 GTGGCAATGA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//