LOCUS       MR231761                8796 bp    DNA     circular  SYN 
DEFINITION  Arhgap33 (myc-DDK-tagged) - Mouse Rho GTPase activating protein 33 (Arhgap33), transcript variant 2
ACCESSION   NM_001289682
VERSION     NM_001289682.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4953..5044 
                     /label="Myc-DDK"  
     misc_feature    5074..5659 
                     /label="PolyA signal"  
     misc_feature    5131..5150 
                     /label="Restriction Enzyme"  
     misc_feature    5935..6607 
                     /label="ColE1"  
     misc_feature    7037..7831 
                     /label="Kan/Neo"  
     misc_feature    7866..8168 
                     /label="SV40 ori"  
     misc_feature    8254..8731 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4935..4941 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4935 
                     /label="Arhgap33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCCAGGCA CAGAAACAGT CAGATCCCAT CCTGCCTTGG GGAGCTTCAT 
     1081 GGGCTGGCAG GGGACAGACT CTGAGGGCCC GCAGCACTGA CAGCCTGGAT GGCCCAGGGG 
     1141 AGGGCTCAGT GCAGCCCGTA CCCACTACTG GGGGGCCGGG TACAAAGGGG AAGCCTGGGA 
     1201 AGAGGCTCTC AGCTCCTCGA GGCCCTTTCC CCCGGCTGGC TGACTGTGCC CATTTCCACT 
     1261 ATGAGAATGT TGACTTTGGC CACATTCAGC TCCTGCTGTC TCCAGAGCGT GAGGGCCCCA 
     1321 GCCTCTCTGG AGAAAATGAG CTGGTCTTTG GGGTACAGGT GACCTGTCAG GGCCGCTCCT 
     1381 GGCCAGTCCT CCGGAGCTAT GACGATTTCC GTTCTCTGGA TGCCCATCTC CACCGGTGCA 
     1441 TATTTGACCG GAGATTTTCC TGCCTCCCAG AGCTCCCTCC ACCTCCAGAG GGTACCAGGG 
     1501 CTGCCCAGAT GCTGGTACCG CTGCTGCTGC AGTACCTGGA GACCCTGTCA GGACTGGTGG 
     1561 ACAGTAACCT CAACTGTGGA CCTGTGCTCA CTTGGATGGA GCTGGACAAC CATGGCCGGC 
     1621 GCCTGCTCCT CAGTGAGGAA GCCTCCCTCA ATATCCCTGC TGTGGCTGCT GCCCACGTGG 
     1681 TCAAGCGGTA CACTGCCCAG GCGCCAGATG AGCTGTCCTT TGAGGTGGGA GACATTGTCT 
     1741 CAGTGATCGA CATGCCACCT ACAGAGGATC GGAGCTGGTG GCGGGGCAAA CGGGGCTTCC 
     1801 AGGTTGGTTT CTTCCCCAGT GAGTGTGTGG AACTCTTCAC GGAGAGGCCA GGGCCTGGCC 
     1861 TAAAGGCAGA TGCTGACAGT CCCCTGTGTG GCATCCCAGC TCCCCAGGGC ATCTCTTCTC 
     1921 TGACCTCAGC TGTGCCCCGG CCACGTGGGA AGCTGGCAGG ACTCCTCCGC ACCTTCATGC 
     1981 GCTCTCGTCC TTCTCGCCAG CGGCTGCGCC AACGGGGAAT CCTGCGGCAG AGGGTGTTTG 
     2041 GTTGTGATCT TGGAGAGCAC CTCAGCAACT CAGGCCAAGA CGTGCCCCAA GTGCTGCGCT 
     2101 GCTGCTCTGA GTTTATTGAG GCCCATGGTG TGGTGGATGG AATCTACAGG CTCTCTGGCG 
     2161 TGTCCTCCAA CATCCAGAGG CTTCGGCATG AGTTTGATAG TGAGAGGATC CCTGAGCTGT 
     2221 CTGGCCCCGC CTTCCTACAA GATATCCACA GTGTGTCCTC CCTCTGCAAG CTCTACTTCC 
     2281 GGGAACTGCC CAACCCCTTG CTCACCTACC AGCTCTATGG GAAGTTCAGT GAGGCTATGT 
     2341 CAGTGCCTGG GGAGGAAGAA CGCCTAGTGC GAGTGCATGA CGTCATCCAG CAGCTGCCTC 
     2401 CACCACACTA CAGGACCCTG GAGTACCTGC TGAGGCACCT GGCCCGCATG GCAAGACACA 
     2461 GCGCTAACAC CAGCATGCAT GCCCGCAACC TGGCCATTGT CTGGGCACCC AACCTGCTAC 
     2521 GGTCTATGGA ACTGGAGTCG GTGGGGCTGG GTGGGGCAGC CGCCTTCCGG GAGGTCCGGG 
     2581 TGCAGTCGGT GGTGGTGGAG TTCCTGCTTA CCCACGTGGA GGTTCTGTTC AGCGATACGT 
     2641 TCACCTCTGC TGGCCTGGAC CCTGCAGGTC GCTGCCTCCT TCCCAGACCC AAGTCCCTTG 
     2701 CGGGGAGCAG TCCCTCCACT CGCCTGCTAA CACTGGAAGA AGCCCAGGCA CGAACTCAGG 
     2761 GCCGTCTTGG AACACCTACA GAGCCCACGA CTCCTAAGAC GCCAGCCTCA CCAGTGGAAA 
     2821 GGAGGAAAAG AGAGAGGGCG GAGAAACAAA GGAAGCCAGG AGGTAGCAGC TGGAAGACAT 
     2881 TCTTTGCTCT GGGGCGGGGC CCCAGCATAC CCCGGAAGAA GCCGCTGCCA TGGCTGGGTG 
     2941 GCAGCCGAGC CCCACCACAG CCTTCAGGCA GCAGACCTGA CACTGTCACG CTGAGATCTG 
     3001 CCAAAAGTGA GGAGTCTCTG TCATCCCAGG CCAGCGGGGC TGGCCTCCAG AGGCTGCACC 
     3061 GGCTGCGACG ACCCCACTCC AGCAGCGATG CTTTTCCCGT GGGCCCAGCA CCTGCTGGCT 
     3121 CTTGCGAGAG CTTGTCCTCT TCTTCTTCCT CTTCTTCCTC TTCCTCCTCC TCCTCCTCCT 
     3181 CAGAGTCTTC AGCAGGTGGC CTGGGGCCAC TCTCTGGCTC CCCCTCACAC CGCACCTCAG 
     3241 CCTGGCTAGA TGATGGCGAT GATCTGGATT TCAGTCCACC CCGCTGTCTG GAAGGACTTC 
     3301 GGGGACTTGA CTTTGATCCC CTTACCTTTC GCTGCAGCAG CCCCACCCCA GGGGACCCTG 
     3361 CACCTCCTGC CAGCCCAGCA CCTCCAGCCT CAGCCTCTGC CTTCCCACCT CGGGCAACCC 
     3421 CACAGGCTCT GTCACCCCAT GGACCCACTA AGCCTGCTTC ACCCACTGCC CTGGACATCT 
     3481 CAGAGCCCCT GGCTGTATCA GTACCACCTG CTGTCCTGGA ACTACTGGGA GCTGGAGGAA 
     3541 CGCCTGCCTC AGCCACCCCA ACACCGGCTC TCAGCCCTCA TCTCATCCCC CTGCTGCTGC 
     3601 GTGGAGCTGA GGCCCAGCTA AGTGACACCT GTCAGCAGGA GATCAGCAGC AAGCTAGCAC 
     3661 CAACCCGGGG AGCTCCAGGC CAGCAAAGTC CTGGCGGCAT GGATTCACCG TTATTGCCCC 
     3721 CACCCTTGCC TCTGCTGCGC CCTGGTGGGG CTCCGCCCCC TCCCCCCAAG AACCCAGCTC 
     3781 GTCTCATGGC CCTGGCCTTG GCTGAGCGAG CTCAGCAGGT AGCAGAGCAA CAGAGCCAAC 
     3841 AAGAGCAGGG GGGCACCCCA CCTGCTCCCC ACTCCCCCTT TCGACGCTCT CTGTCCCTAG 
     3901 AGGTGGGTGG GGAGCCTGTG GGGACTTCAG GGAGTGGGAT ACACCCTCCC TCCTTAGCTC 
     3961 ACCCAGGTGC CTGGGCTCCA GGTCCCCCAC CTTACCTCCC AAGGCAACAG AGTGATGGGA 
     4021 GCCTGGTAAG AAGCCAGCGG CCCTTGGGGA CCTCAAGGAG AAGTCCCAGA GGGCCTTCCC 
     4081 AGGTCAGTGC CCATCTCAGG GCAAGCGGGG CTTACAGGGA TGCTCCAGAG ATGGCAGCCC 
     4141 AGTCACCATG TTCTGTCCCC TCACAGGGTT CTAACCCCAG CTTCTTCTCA ACCCCCCGAG 
     4201 AGTGTTTGCC ACCTTTCCTT GGCGTCCCCA AACAAGGCTT GTACTCTCTG GGACCCCCAT 
     4261 CCTTCCCACC TAGCTCTCCA GCCCCAGTCT GGAGGAACTC TCTGGGTGCC CCCTCAGCAT 
     4321 TGGACAGGGG AGAGAATCTG TACTATGAAA TTGGGGTAGG GGAGGGGACC TCCTACTCAG 
     4381 GCCCCAGCCG GTCCTGGAGT CCGTTTCGCT CCATGCCTCC CGACAGGCAT AATGCCTCCT 
     4441 ATGGTATGCT TGGCCAGTCA CCACCGCTCC ACAGGTCCCC TGATTTCCTG CTCAGCTACC 
     4501 CGCCTCCCCC CTCCTGCTTT CCCCCAGAGC ACCTTACCCA CTCAGTTTCA CAGCGCCTTG 
     4561 CCCGGCGCCC TACCCGTCCT GAGCCCCTCT ATGTCAACCT AGCCCTAGGG CCCAGGGGCC 
     4621 CTTCACCTGC CTCTTCTTCC TCTTCCTCTC CTCCTGCCCA CCCCAGGAGC CGGTCAGATC 
     4681 CTGGGCCTCC TGTTCCCCGC CTCCCTCAGA AACAGCGGGC TCCTTGGGGT CCACATACTC 
     4741 CCCATCGGGT GCCTGGACCT TGGGGTTCCC CCGAACCTTT TCTGCTCTAT AGGCCAGCTC 
     4801 CACCATCCTA TGGGAGGGGA GGGGAGGTCC GAGGATCCTT GTACAGAAAT GGAGGACATA 
     4861 GAGGAGAGGG GGCTGGTCCC CCTCCTCCTT ACCCCACACC CAGCTGGTCC TTACATTCAG 
     4921 AAGGACAGAC CAGAAGTTAC TGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//