LOCUS       MR231757                8787 bp    DNA     circular  SYN 
DEFINITION  Astn1 (myc-DDK-tagged) - Mouse astrotactin 1 (Astn1), transcript variant 1
ACCESSION   NM_001205204
VERSION     NM_001205204.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4944..5035 
                     /label="Myc-DDK"  
     misc_feature    5065..5650 
                     /label="PolyA signal"  
     misc_feature    5122..5141 
                     /label="Restriction Enzyme"  
     misc_feature    5926..6598 
                     /label="ColE1"  
     misc_feature    7028..7822 
                     /label="Kan/Neo"  
     misc_feature    7857..8159 
                     /label="SV40 ori"  
     misc_feature    8245..8722 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4926..4932 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4926 
                     /label="Astn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTAGCC GGGCTCTGCG CCCTGTTCGC CTGCTGCTGG GGGCCGGCTG 
     1081 CGGTGCTGGC CACAGCTGCC GGCGACGTGG ATCCATCCAA GGAGCTGGAG TGCAAGCTCA 
     1141 AGAGCATCAC TGTGTCGGCA CTGCCCTTCC TGCGCGAGAA CGACCTGAGC ATCATGCACA 
     1201 GTCCCTCGGC CTCCGAACCC AAGCTTCTCT TCTCCGTGCG CAATGACTTC CCGGGAGAAA 
     1261 TGGTCGTGGT GGACGACCTA GAGAACACGG AGCTACCCTA TTTCGTGCTG GAGATCTCAG 
     1321 GGAATACAGA AGACATCCCC CTGGTGCGCT GGAGGCAGCA ATGGCTGGAG AATGGCACTT 
     1381 TACTCTTTCA TATTCACCAC CAAGATGGTG CTCCAAGTCT CCCTGGACAA GACCCAACTG 
     1441 AAGAACCCCA ACATGAATCA GCAGAGGAAG AGCTGAGGAT CCTGCACATC TCAGTCATGG 
     1501 GTGGCATGAT CGCTCTGTTG CTATCCATCT TGTGCCTGGT GATGATCCTG TACACTCGCA 
     1561 GGCGCTGGTG CAAACGTCGC CGGGTTCCCC AGCCCCAGAA GAGTGCCAGT GCAGAGGCAG 
     1621 CAAATGAGAT TCACTACATC CCATCTGTGC TGATTGGAGG CCATGGGCGG GAAAGCTTGC 
     1681 GCAATGCCCG TGTGCAGGGC CACAACTCCA GTGGCACTCT GAGCATTCGG GAGACACCCA 
     1741 TCCTGGATGG CTATGAGTAT GACATCACAG ACCTGCGTCA CCACCTGCAA AGGGAGTGTA 
     1801 TGAATGGAGG GGAGGACTTT GCCAGTCAGG TCACACGCAC TTTGGACTCC CTACAGGGAT 
     1861 GCAATGAGAA GTCTGGGATG GATCTAACAC CAGGGAGTGA CAATGCCAAG CTTTCCCTGA 
     1921 TGAACAAGTA TAAAGACAAT ATCATAGCCA CAAGCCCCGT GGATTCTAAC CACCAGCAAG 
     1981 CCACTCTGCT CTCCCACACC TCCAGCAGCC AGAGAAAGAG GATCAACAAT AAAGCCAGAG 
     2041 CTGGTTCTGC CTTCTTGAAC CCTGAAGGGG ACTCCAGCAC AGAGGCCGAA AATGACCCAC 
     2101 AGCTGACCTT CTACACAGAC CCCTCTCGGA GCCGGAGGCG CAGTCGAGTG GGCTCTCCCC 
     2161 GAAGTCCTGT GAATAAGACC ACCTTGACCC TGATCAGTGT CACCAGCTGC GTGATTGGCC 
     2221 TCGTGTGCTC TTCTCATGTC AGCTGCCCTC TTGTTGTCAA GATCACCCTA CACGTCCCTG 
     2281 AGCACTTGAT CGCTGATGGG AGCCGCTTCA TCCTGCTGGA GGGGAGCCAG CTGGATGCCA 
     2341 GTGACTGGCT GAACCCTGCC CAAGTGGTTC TCTTCTCTCA GCAGAACTCC AGCGGGCCCT 
     2401 GGGCCATGGA CCTCTGTGCC CGGCGGCTCC TGGACCCTTG TGAACACCAA TGTGACCCCG 
     2461 AAACTGGTAG GCGGGAGCAC CGGGCAGCGG GGGAATGCCT GTGCTATGAA GGCTACATGA 
     2521 AGGATCCTGT CCACAAGCAC CTTTGCATTC GGAATGAATG GGGGACCAAC CAGGGGCCTT 
     2581 GGCCTTACAC GATATTTCAG CGAGGCTTTG ACCTGGTTTT GGGAGAGCAA CCCTCTGATA 
     2641 AGATATTCAG ATTCACCTAT ACCCTCGGGG AAGGCATGTG GTTACCCCTC AGTAAGAGCT 
     2701 TTGTGATTCC ACCAGCCGAA CTAGCCATCA ATCCATCAGC AAAGTGTAAG ACGGATATGA 
     2761 CTGTGATGGA AGATGCTGTA GAGGTCAGAG AGGAGCTGAT GACATCATCA TCCTTTGACA 
     2821 GCCTGGAGGT CCTATTAGAC TCCTTTGGGC CAGTGCGTGA TTGCAGCAAA GATAACGGAG 
     2881 GCTGCAGTAA GAATTTTCGC TGCATTTCAG ATCGCAAGTT GGACTCTACT GGTTGTGTGT 
     2941 GTCCTTCAGG ACTCAGCCCT ATGAAGGACA GCTCAGGCTG CTATGATCGC CATATCGGAG 
     3001 TGGACTGCTC AGATGGTTTC AACGGCGGCT GCGAACAGCT GTGTCTCCAG CAGATGGCGC 
     3061 CGTTCCCAGA GGACCCCACC TTGTACAACA TCCTTATGTT CTGCGGGTGT ATCGAAGACT 
     3121 ACAAGCTTGG TGTGGATGGA CGCTCTTGCC AACTTGTCAC TGAGACCTGC CCAGAGGGAG 
     3181 GTGACTGTGG GGAAAGCAGA GAGGTTCCCA TGAACCAGAC TCTCTTTGGA GAAATGTTCT 
     3241 TTGGCTACAA CAACCAGTCC AAGGAAGTAG CCACTGGACA GGTGCTAAAA GGAACATTCA 
     3301 GACAGAACAA CTTTGCTCGT GGTTTAGACC AGCAACTGCC GGATGGTCTT GTGGTTGCCT 
     3361 CTGTCCCACT GGAGAATCAG TGCCTAGAGG AAATCTCAGA GCCCACCCCA GACCCTGACT 
     3421 TCTTGACTGG GATGGTGAAC TTCAGTGAAG TGTCTGGATA CCCGGTGCTG CAGCACTGGA 
     3481 AGGTTCGGTC TGTGATGTAC CACATCAAAC TCAACCAAGC AGCCATCTCG CAGGCCTTCA 
     3541 GCAATGCTCT TCACTCCTTG GATGGGGCTA CATCTCGTGC AGATTTTGTG GCTTTGTTGG 
     3601 ATCAGTTTGG AAACCATTAC ATCCAGGAAG CTGTCTACGG CTTTGAGGAA TCCTGTTCTA 
     3661 TATGGTACCC AAACAAGCAA GTCCAGCGGA GACTCTGGCT GGAATATGAA GACATCAGTA 
     3721 AAGGCAACTC TCCATCTGAT GAGTCGGAGG AGCGGGAAAG GGATCCCAAG GTGCTGACAT 
     3781 TCCCAGAATA CATCGCTAGC CTGTCAGACT CTGGCACCAA GCGAATGGCA GCTGGAGTCC 
     3841 GGATGGAGTG CCAGAGCAAG GGACGGTGTC CCTCATCCTG TCCTTTATGT CATGTGACAT 
     3901 CCAGCCCTGA AACCCCTGCT GAGCCAGTTC TACTGGAAGT GACCAGAGCA TCCCCCATCT 
     3961 ATGAACTGGT GACCAATAAC CAGACCCAGA GGCTCTTACA GGAAGCCACC ATGAGCTCTC 
     4021 TCTGGTGTTC AGGGACCGGA GATGTCATTG AGGACTGGTG CCGATGCGAC TCGACTGCTT 
     4081 TTGGAGCAGA TGGACTTCCT ACCTGTGCAC CCCTCCCACA GCCTGTGCTG AGACTTTCCA 
     4141 CAGTACACGA GCCCAGCAGT AACCTCGTGG TCCTGGAGTG GGAACATTCA GAGCCGCCAA 
     4201 TTGGGGTGCA GATTGTAGAC TACCTGATCC GGCAAGAGAA AGTCACTGAC CGGATGGACC 
     4261 ACTCCAAAGT AGAGACGGAA ACAGTGCTGA GCTTTGTAGA CGACATCATC TCTGGAGCAA 
     4321 AGGCTCCATG TGCCATGCCG TCTCAGGTGC CAGACAAACA GCTCACCACG ATTTCTCTCA 
     4381 TCATCCGATG CCTGGAACCT GACACCATTT ACATGTTCAC CCTCTGGGGA GTAGATAACA 
     4441 CAGGGCGACG TTCCAGGCCA AGTGATGTGA TTGTGAAGAC CCCATGTCCT GTGGTGGATG 
     4501 ATGTCAAAGC CCAAGAAATA GCAGACAAGA TCTACAATCT CTTCAATGGC TACACCAGTG 
     4561 GAAAGGAGCA ACAGACTGCC TACAACACCC TTCTGGATCT GGGTTCTCCC ACTTTGCACC 
     4621 GAGTCCTCTA CCACTATAAC CAGCACTATG AGAGTTTTGG GGAATTCACC TGGCGGTGTG 
     4681 AAGATGAGTT AGGACCCAGG AAAGCAGGCC TCATCCTTTC CCAGCTTGGA GATCTGAGCA 
     4741 GCTGGTGCAA TGGACTCCTT CAGGAGCCCA AGATAAGCTT GAGGCGTGGT TCACTCAAGT 
     4801 ACCTGGGCTG CCGCTACAGT GAGATCAAAC CCTATGGACT GGACTGGTCA GAGCTCAGTC 
     4861 GGGACCTCAG GAAGACATGT GAAGAACAGA CCCTGAGTGT CCCCTACAAT GACTACGGGG 
     4921 ACAGCAAAGA CATCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4981 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5041 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5101 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5161 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5221 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5281 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5341 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5401 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5461 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5521 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5581 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5641 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5701 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5761 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5821 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5881 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5941 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6001 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6061 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6121 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6181 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6241 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6301 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6361 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6421 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6481 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6541 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6601 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6661 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6721 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6781 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6841 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6901 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6961 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7021 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7081 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7141 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7201 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7261 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7321 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7381 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7441 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7501 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7561 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7621 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7681 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7741 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7801 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7861 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7921 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7981 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8161 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8221 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8281 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8341 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8401 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8461 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8521 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8581 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8641 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8701 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8761 ATTTAACAAA AATTTAACGC GAATTTT
//