LOCUS       MR231754                8745 bp    DNA     circular  SYN 
DEFINITION  Map4k4 (myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1
ACCESSION   NM_001252200
VERSION     NM_001252200.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="Map4k4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAACGAC TCTCCCGCGA AGAGCCTGGT GGACATTGAC CTGTCGTCCC 
     1081 TGCGGGACCC TGCTGGGATT TTTGAGCTGG TGGAAGTGGT TGGAAATGGC ACCTATGGAC 
     1141 AAGTCTATAA GGGTCGACAT GTTAAAACGG GTCAGCTGGC CGCCATCAAG GTTATGGACG 
     1201 TCACCGAGGA TGAAGAGGAA GAAATCAAAC TGGAGATAAA TATGCTGAAG AAGTATTCTC 
     1261 ATCATCGAAA TATTGCCACG TACTATGGTG CTTTCATTAA GAAGAGCCCT CCAGGACATG 
     1321 ATGACCAACT CTGGCTTGTT ATGGAGTTTT GTGGGGCTGG GTCCATCACA GACCTTGTGA 
     1381 AGAACACCAA AGGGAACACT CTCAAAGAAG ACTGGATTGC TTACATCTCC AGGGAAATCC 
     1441 TCAGGGGATT GGCACATCTC CATATTCACC ACGTTATTCA CCGAGATATC AAGGGCCAAA 
     1501 ATGTGCTGCT GACCGAGAAT GCTGAGGTGA AACTTGTTGA TTTTGGTGTA AGCGCTCAGC 
     1561 TGGACAGGAC GGTTGGACGG AGAAATACGT TCATAGGCAC ACCCTACTGG ATGGCTCCAG 
     1621 AGGTCATCGC CTGTGATGAG AACCCAGACG CCACTTACGA CTACAGAAGT GACCTCTGGT 
     1681 CCTGTGGCAT CACAGCCATC GAGATGGCTG AAGGGGCCCC CCCTCTCTGT GACATGCATC 
     1741 CAATGAGAGC GCTGTTTCTC ATCCCCAGAA ACCCTCCTCC CAGGCTGAAG TCAAAAAAAT 
     1801 GGTCAAAGAA ATTTTTCAGC TTTATAGAAG GCTGTCTGGT GAAGAATTAC ATGCAGCGGC 
     1861 CCTCTACAGA GCAACTTTTA AAACACCCTT TCATAAGGGA TCAGCCCAAT GAAAGGCAGG 
     1921 TTCGAATCCA GCTTAAGGAT CACATAGACC GGACCAGAAA GAAGAGAGGC GAGAAAGATG 
     1981 AGACGGAGTA CGAGTACAGC GGGAGCGAGG AGGAGGAGGA GGAAGTGCCT GAGCAGGAGG 
     2041 GAGAGCCAAG TTCCATCGTC AATGTGCCTG GAGAGTCAAC TCTGCGACGT GATTTCCTGA 
     2101 GACTGCAGCA GGAGAACAAG GAGCGGTCTG AGGCTCTGCG GAGACAGCAG CTTCTGCAGG 
     2161 AGCAGCAGCT CCGGGAGCAG GAGGAGTATA AGAGGCAGCT GCTGGCTGAG AGGCAGAAGC 
     2221 GGATTGAACA GCAGAAAGAA CAGAGGAGGC GGCTGGAAGA GCAACAAAGA AGAGAACGGG 
     2281 AAGCCAGGAG GCAGCAGGAG CGTGAGCAGC GGCGGCGTGA ACAAGAGGAG AAGAGGCGTC 
     2341 TCGAGGAACT GGAAAGGCGG CGTAAAGAAG AGGAAGAGAG GAGACGGGCA GAAGAGGAGA 
     2401 AGAGGAGAGT GGAGAGGGAA CAGGAGTACA TCAGGCGGCA GCTAGAGGAG GAGCAGCGGC 
     2461 ACCTGGAGAT CCTGCAGCAG CAGCTGCTCC AGGAGCAGGC CATGTTACTG CACGACCACA 
     2521 GGAGGCCGCA CGCACAGCAG CAGCCGCCGC CCCCGCAGCA GCAGGACAGG AGCAAACCGA 
     2581 GCTTTCATGC TCCAGAGCCC AAGCCTCACT ATGACCCTGC TGACAGAGCT CGGGAGGTGG 
     2641 AAGATAGATT TAGAAAGACT AACCACAGCT CCCCTGAGGC CCAGGCTAAG CAAACGGGCA 
     2701 GAGGACTGGA GCCACCCGTG CCTTCCCGGT CAGAGTCTTT TTCCAATGGC AACTCCGAGT 
     2761 CTGTGCACCC TGCCCTGCAG AGACCAGCGG AGCCACAGGT ACAGTGGTCC CACCTGGCAT 
     2821 CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCCA 
     2881 AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC AGTGAGGGGC 
     2941 TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG GCTTGGTCTA 
     3001 GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT CGTTCCCCTG 
     3061 TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC CAAGCAGGAC 
     3121 AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG GAAAAGCTGG 
     3181 TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT 
     3241 CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT 
     3301 CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC GATAAGAAAG 
     3361 AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC 
     3421 TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA TCCTCCAGTG 
     3481 AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG GCTGATGATT 
     3541 CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC AACGGTGAAA 
     3601 CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA GCCATGACCC 
     3661 CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGACTCAGTC CGCTAGTAGC ACACTCCAGA 
     3721 AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA CAGATCTCTC 
     3781 CATCTAGTGG GACGACAGTG ACTTCCGTGG TGGGATTTTC CTGTGATGGG CTGAGACCAG 
     3841 AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC CCCACGAACA 
     3901 CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT AACTCAGAGA 
     3961 TCCTGTGTGC TGCCTTATGG GGAGTGAACT TGCTGGTTGG TACAGAGAGC GGTCTGATGC 
     4021 TCTTGGACAG AAGTGGCCAA GGGAAAGTAT ACCCTCTGAT CAGCCGAAGA CGGTTCCAGC 
     4081 AAATGGATGT GCTCGAAGGC CTAAATGTCC TGGTGACAAT ATCTGGCAAA AAGGATAAGT 
     4141 TACGTGTCTA CTATTTATCC TGGCTAAGAA ATAAGATTCT TCATAATGAT CCAGAGGTTG 
     4201 AGAAGAAGCA GGGGTGGACC ACTGTGGGCG ACTTGGAAGG GTGCGTGCAC TATAAAGTCG 
     4261 TAAAATATGA AAGAATCAAG TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG 
     4321 CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA GAACTGCTAC 
     4381 ATAAGCCATT ACTGGTGGAT CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG 
     4441 GATCCTGTGC GGGATTCCAT GCTGTTGATG TGGATTCAGG CTCAGTCTAT GACATTTATC 
     4501 TACCAACACA TATTCAGTGT AGCATCAAAC CCCATGCAAT CATTATCCTC CCCAACACGG 
     4561 ATGGGATGGA GCTGCTGGTG TGCTACGAAG ACGAGGGGGT TTACGTTAAC ACGTACGGAA 
     4621 GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC GACATCTGTA GCATATATCC 
     4681 GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGATCT GTGGAAACTG 
     4741 GTCACTTGGA TGGTGTATTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC CTGTGTGAAC 
     4801 GCAATGACAA GGTGTTCTTT GCCTCCGTCC GCTCTGGTGG CAGCAGCCAG GTCTATTTCA 
     4861 TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//