LOCUS       MR231753                8742 bp    DNA     circular  SYN 
DEFINITION  Pex1 (myc-DDK-tagged) - Mouse peroxisomal biogenesis factor 1 (Pex1), transcript variant 1
ACCESSION   NM_001293806
VERSION     NM_001293806.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4899..4990 
                     /label="Myc-DDK"  
     misc_feature    5020..5605 
                     /label="PolyA signal"  
     misc_feature    5077..5096 
                     /label="Restriction Enzyme"  
     misc_feature    5881..6553 
                     /label="ColE1"  
     misc_feature    6983..7777 
                     /label="Kan/Neo"  
     misc_feature    7812..8114 
                     /label="SV40 ori"  
     misc_feature    8200..8677 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4881..4887 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4881 
                     /label="Pex1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG TGGAGCAGCG ATCGCTTAGC GGGTGCGGGG TCTGGTGGGG 
     1081 CGGTCGTGAC GGTGGCCTTC ACGAATGCTC GGGACTGCTT CCTGCATCTG CCGCGGCGCC 
     1141 TCGTGGCCCA GCTTCACCTG CTGCAGAATC AAGCTATAGA AGTGGCCAGC GATCACCAGC 
     1201 CTACCTACCT GAGCTGGGTG GAAGGCAGGC ATTTTAATGA TCAAAGTGAA AATGTGGCAG 
     1261 AAATTAATAG ACAAGTTGGC CAGAAACTTG GACTCTCAAG CGGAGATCAG GTGTTTCTCA 
     1321 GGCCTTGTTC CCATGTGGTA TCTTGCCAAC AGGTTGAAGT GGAGCCTCTC TCAGCAGATG 
     1381 ACTGGGAAAT ACTGGAGCTG CACGCCATTT CCCTTGAACA GCATCTTCTG GATCAGATTC 
     1441 GAATAGTTTT CCCCAAAGCT GTTGTTCCCA TCTGGGTTGA CCAGCAGACC TACATATTTA 
     1501 TCCAGATCGT CACTCTGATG CCAGCTGCCC CTTATGGAAG GCTAGAAACT AACACCAAAC 
     1561 TCCTTATTCA GCCAAAGACA CGCCAAGCCA AAGAGAGCAC ATTTCCAAAA GAAGGAGATG 
     1621 CACACGGACA AGTTCATAGT TATGGGCGAG AACAGAAAGG ATTGTCAAAG GAATTACAAA 
     1681 CCAGGCAGCT TCATACGAAC TCAGAGGGTA TCACTGCATC CAATGGAAGA GACCCAAAAG 
     1741 TCCCAGGTGG CCCGTTGAAG CCAAGCTGGT GGGCTGTGCT CGGAAGCATG CTTTCCTTTG 
     1801 GGCCTGACAG TAAACAGGAG TCAGCCTGGG GCTCGTTGGA ACTCGGTGCT TTCAAAAACA 
     1861 TGCAGTCACA GGCTGCCCCT CTGGAAGGTA CTTTCAGAGT ATGCCAAGTC CAGCCACCTA 
     1921 GTGCGCGTAC CACCACCGCT ACCTCTGTGT TTCACAAACA CTGCACCGCC CACGTATTTC 
     1981 CTTGGGACCA AGAATACTTT GATGTGGAGC CCAGCTTTAC TGTGACCTAT GGAAAACTAG 
     2041 TTAAGCTACA TTCTCCAAAA CAACAGCAAG ACAAAAGTAA GCAGGGTGTC CTGTTGCCTG 
     2101 ACAAAGAGAA GCAGCTGTCC AAGTCTCCAG ATCATAAGCA GATCAGCTCC AACCGCAGTG 
     2161 AGGAGGCTGC TGAGGCCTGT GTGCTGAAGG TAGTCTGGAA TGGACTCGAG GAGTTGAAGA 
     2221 ATGCCACAGA GTTCACCGAA AGTCTAGAGC TTCTCCACCG TGGGAAAGTC TGGATTCCAG 
     2281 ATGACCTGAG AAAGAGACTA AATATAGAAA TGCATGCAGT AGTCAGAATA ACTCCACTGG 
     2341 AAACCACGCC TAAAATCCCA AGATCTCTGA AATTACAACC TAGAGAGAAC TTACCTAAAG 
     2401 ACGTCAATGA AGAAACTATA AAAACAGTGT TCTCATCTTG GGTACAGCAG TCCGCTACCA 
     2461 CGATGCTTCC TTTGGTAATA TCAAAGGAAG AGCGTATTAA GCTGGAAATT AAAGATGGGC 
     2521 TGAGAGAGTT CTCTCTGAGT ACAGTTCATT CTCAGGAAAA AGAAAAGGAA GAAGGAAAAA 
     2581 CTGTGTTTGT GTTGAGTTCC ATCCTGCTGC AGAAGATCTC AGTACAAGTC CTTCTAGAGC 
     2641 CCATGATAAA AGAAGAACAG AGTGCGGAAA TCGACTTTCT TCTTCCCTCT TTAACGCTGA 
     2701 GCTCTTTGGG GGGAGTGAGT GCCTTAGGTG CATCTGCAAT GGAGCACATC ACTCACAGTC 
     2761 TCCTGGGACG CCCGTTGTCT AGGCAGCTGA TGGCCCTCGT TGCAGGACTT AGGAATGGCG 
     2821 CTCTTTTGAT CACTGGAGGA AAGGGAAGCG GGAAGTCAAC ATTCGCGAAA GCCATCTGCA 
     2881 AGGAAGCACA GGACACTCTG GATGCCCGTG TGGAGACAGT CGACTGCAAG GCTTTACGAG 
     2941 GAAAAAGGCT TGAAAGCATA CAAAAAGCGC TAGAGGTGGC TTTCTCAGAG GCCGCCTGGA 
     3001 GGCAGCCATC TGTCATTCTG CTGGATGACC TGGACCTCAT TGCCGGACTG CCAAGTGTCC 
     3061 CCGAGCAGGA GCACAGCCCT GAAGCGGTGC AGAGCCAGCG GCTTGCACAT GCTTTGAACG 
     3121 ATATGATCAA AGAGTTTGTT TCCACGGGAA GCTTGGTGGC ACTCATCGCC ACGAGCCAGC 
     3181 TCCAGCAGTC TCTGCACCCT TCCCTTGTGT CTGCTCAAGG AATCCACACG TTTCAATGTG 
     3241 TCCAGCACCT TCAGCCTCCC AATCCGGAAC AGAGATGTGA AATTCTGCAC AGTGTTGTGA 
     3301 AGAATAAACT GGGCTGTGAT ATAAGCAATT TCCCTGACTT GGACCTGCAG TGCATAGCTA 
     3361 AAGACACAGA AGCGTTTGTG GCTCGTGACT TTACAGTTCT TGTGGACCGA GCCATACACT 
     3421 CTTCTCTCTC TCGCCAGCAT AGCTCCTCTA GGGAAGACTT GACTTTAACA ACATCAGACT 
     3481 TCCAAAAGGC TCTCCGTGGA TTCCTTCCTG CTTCTCTGCG AAATGTCAAC TTGCATAAAC 
     3541 CTAGAGACCT GGGCTGGGAC AAGATTGGTG GATTACATGA AGTTCGGCAG ATCCTCATGG 
     3601 ATACTATCCA GTTACCAGCC AAGTACCCAG AATTATTTGC AAACTTACCC ATACGACAGA 
     3661 GGACAGGAAT ACTGCTTTAT GGTCCTCCAG GGACAGGAAA AACTTTACTT GCTGGGGTAG 
     3721 TTGCAAGAGA GAGTGGAATG AATTTTATTA GTATTAAGGG ACCAGAGTTA CTCAGCAAAT 
     3781 ATATTGGCGC AAGTGAGCAA GCTGTTCGAG ATGTTTTCAT CAGAGCACAG GCTGCAAAGC 
     3841 CCTGCATTCT TTTCTTTGAT GAGTTTGAGT CCATCGCTCC TCGAAGAGGC CATGACAACA 
     3901 CAGGGGTTAC AGACCGAGTA GTCAACCAGT TGCTGACACA GTTAGACGGA GTAGAAGGCT 
     3961 TACAGGGAGT TTATGTGCTG GCTGCTACTA GTCGCCCTGA CTTGATCGAC CCTGCCCTGT 
     4021 TGCGGCCTGG CAGACTGGAT AAATGTGTAT ACTGCCCTCC TCCAGATCAG GTGTCCCGTC 
     4081 TTGAGATTTT AACTGTCCTC AGCAAGTCTC TAGCTCTGGC AGATGACGTG GACCTTCAGC 
     4141 ACGTGGCGTC GGTCACCGAC TCGTTCACTG GAGCGGATCT GAAAGCTCTG CTGTACAACG 
     4201 CTCAGCTGGA GGCCTTGCAG GGACGGCTGC TGCCCAGTGG GCTTCCCGAT GGAGGCTCCA 
     4261 GCTCTGACAG TGACCTGAGT CTGTCTTCAA TGGTCTTTCT TAACCACAGC AGTGGTTCCG 
     4321 ACGACTCCGC TGGAGATGGA GAATGTGGCT TAGAGCAATC CCTGCTTTCT CTCGAGATGT 
     4381 CTGAGATCCT TCCAGACGAA TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCGT 
     4441 ATGAATCGGA GCTTGGAAAT GGGACCCCTT CTGACTTGAG CTCACACTGT CTGTCTGCAC 
     4501 CAAGCTCCGT GACTCAGGAT TTACCTGCAG CTCCTGGGAA AGACCCGTTA TTTACACAAC 
     4561 ATCCTGTGTT CAGGACACCT TCCCAAGAAG GCTGCCAAGA CCTCACCCAG GAGCAGAGAG 
     4621 ATCAGCTGAG GGCAGAGATC AGCATCATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG 
     4681 ATGAATCCCT TAACCAGCCT GGACCAATCA AAACCACTTT TGCTATTAGC CAGGCACATT 
     4741 TAATGACTGC ACTTGCCCAC ACAAGACCGT CTATTAGTGA AGATGAAGGG AAGGAATTTG 
     4801 CTGAGCTGTA TGAGAACTTT CAAAATCCAA AGAAGAGAAA AAATCAAAGT GGAACAGTGT 
     4861 TTCGAACTGG ACAGAAAGTA ACTTTAGCAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4921 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4981 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5041 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5101 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5161 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5221 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5281 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5341 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5401 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5461 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5521 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5581 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5641 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5701 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5761 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5821 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5881 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5941 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6001 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6061 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6121 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6181 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6241 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6301 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6361 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6421 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6481 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6541 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6601 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6661 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6721 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6781 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6841 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6901 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6961 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7021 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7081 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7141 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7201 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7261 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7321 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7381 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7441 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7501 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7561 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7621 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7681 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7741 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7801 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7861 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7921 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7981 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8041 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8161 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8221 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8281 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8341 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8401 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8461 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8521 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8581 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8641 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8701 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//