LOCUS       MR231748                8706 bp    DNA     circular  SYN 
DEFINITION  Nek1 (myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 1
ACCESSION   NM_001293637
VERSION     NM_001293637.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4863..4954 
                     /label="Myc-DDK"  
     misc_feature    4984..5569 
                     /label="PolyA signal"  
     misc_feature    5041..5060 
                     /label="Restriction Enzyme"  
     misc_feature    5845..6517 
                     /label="ColE1"  
     misc_feature    6947..7741 
                     /label="Kan/Neo"  
     misc_feature    7776..8078 
                     /label="SV40 ori"  
     misc_feature    8164..8641 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4845..4851 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4845 
                     /label="Nek1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTGAGACTGC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CTGTTCTTGT TAAATCGACA GAGGATGGCA GACATTATGT CATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC TGATAAAGAA AGGCAAGAAT CAAGGAGAGA AGTTGCTGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAATATAAAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAA GGAGGTGATT TGTTTAAACG AATAAATGCT CAGAAAGGCG 
     1321 CTCTGTTTCA AGAAGACCAG ATTTTGGACT GGTTTGTGCA GATATGTTTG GCTCTGAAGC 
     1381 ATGTACATGA TAGAAAAATT CTTCACCGAG ACATAAAGTC ACAGAACATA TTTCTAACCA 
     1441 AAGATGGGAC AGTGCAGCTT GGAGATTTTG GAATTGCTCG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGCACTCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AGCCTTATAA CAATAAAAGT GACATTTGGG CTTTGGGCTG TGTCCTTTAT GAGTTGTGTA 
     1621 CACTTAAACA TGCATTTGAA GCTGGAAACA TGAAAAACCT GGTACTGAAG ATAATCTCCG 
     1681 GATCCTTTCC TCCAGTGTCT CCACATTACT CCTATGATCT CCGCAGCTTG CTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCTAAACG AATCGAAAAG TTTCTCTCCC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACACTTTC AAAGTTTGGA CCACAGCCTC TCCCAGGTAA AAGACCAGCA TCAGGACAAG 
     1921 GTGTCAGTTC TTTTGTCCCT GCTCAGAAAA TCACAAAGCC TGCTGCTAAA TACGGAGTGC 
     1981 CTTTAACATA TAAGAAGTAT GGAGATAAAA AGTTACTTGA GAAAAAACCA CCCCCAAAAC 
     2041 ATAAACAGGC CCATCAAATT CCCGTGAAGA AAATGAATTC TGGAGAAGAA AGGAAGAAAA 
     2101 TGTCTGAGGA AGCAGCAAAA AAAAGAAGGT TGGAATTTAT TGAGAAAGAA AAGAAGCAAA 
     2161 AGGATCAGAT TAGGTTCCTG AAGGCTGAGC AGATGAAGCG GCAAGAGAAG CAGCGGTTGG 
     2221 AGAGGATAAA TAGGGCCAGG GAACAAGGAT GGAGGAATGT TTTAAGGGCT GGTGGAAGCG 
     2281 GTGAAGTAAA GGCTTCCTTT TTTGGCATTG GAGGGGCTGT CTCTCCATCA CCGTGTTCTC 
     2341 CTCGAGGCCA GTATGAACAT TACCATGCCA TTTTTGACCA AATGCAGCGG CTAAGAGCAG 
     2401 AAGATAATGA AGCAAGATGG AAGGGGGGAA TCTATGGTCG ATGGCTCCCA GAAAGAGGAG 
     2461 TTCCACCTGG AGTTCGTCCA GGATTTCCCA GTGAGGCTTC AGGTCACTAC CATTCCCCTG 
     2521 ATGCTGGTGC TATTAGGAAA ACTCTGAAAA GACTGAAGGC CGTGTCTAAG CAAGCCAGTA 
     2581 CAAACAGGCA AAAAGGACAC TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC 
     2641 AGCGTAAACG AGAAGCTATG CAGAATAAAG CCCGAGCCGA AGGACACGTG GGACTCCTGC 
     2701 AAAACCTGGC ATCTCTGTAT GGAGGCAGGC CCAGCTCTTC AAGAGGAGGG AAGCCAAGGA 
     2761 ACAATGAGGA AGAGGTTTAT TTGGCAAGAC TGAGGCAAAT AAGACTACAA AATTTTAATG 
     2821 AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAGAATAA AGAAGCTGAT GGTACCAAAG 
     2881 GACAAGAAGC AACTGAAGAG ACTGACATGA GGCTCAAAAA GATGGAGTCA CTTAAGGCGC 
     2941 AAACAAATGC ACGTGCTGCT GTACTAAAAG AACAGCTGGA GCGAAAAAGA AAGGAAGCTT 
     3001 ATGAAAGAGA AAAGAAAGTA TGGGAAGAAC ATTTGGTGGC GAGGGTAAAA AGCTCAGATG 
     3061 TTCCTCTGCC TTTGGAACTT CTTGAAACAG GTGGTTCTCC ATCAAAGCAG CAGGTGAAGC 
     3121 CTGTCATTTC TGTGACTTCA GCTTTGAAAG AAGTGGGCCT GGATGGAAGT TTAACTGATA 
     3181 CCCAGGAAGA AGAAATGGAA AAGAGTAACA GTGCTATTTC AAGTAAGCGA GAAATCCTGC 
     3241 GTAGGCTAAA TGAAAATCTT AAAGCTCAAG AGGATGAAAA GGAAAAGCAG CATCACTCAG 
     3301 GTTCTTGTGA GACCGTTGGT CACAAAGATG AGAGAGAGTA TGAGACAGAA AATGCCATTT 
     3361 CCTCTGATCG CAAGAAGTGG GAGATGGGAG GTCAGCTTGT GATTCCTCTC GATGCAGTGA 
     3421 CACTGGATAC ATCCTTCTCT GCAACCGAAA AACATACTGT GGGAGAGGTT ATTAAATTAG 
     3481 ATTCTAATGG CTCTCCAAGA AAAGTCTGGG GGAAAAACCC TACAGATTCT GTGCTGAAGA 
     3541 TACTTGGAGA AGCTGAATTA CAGCTACAGA CAGAACTACT AGAAAACACA TCTTTTAAAA 
     3601 GTGAGGTTTA TGCTGAAGAG GAGAACTACA AACCCTTACT TACTGAAGAA GAGAATCTGC 
     3661 AGTGCATTTC AAAAGAAATA AATCCATCAG CTACTGTTGA TTCTACTGAA ACGAAAAGTC 
     3721 CAAAGTTTAC TGAGGTGTCT CCACAAATGT CAGAAGGAAA TGTGGAAGAA CCTGATGATT 
     3781 TGGAAACAGA AGTTCTACAA GAGCCAAGTA GCACACACAC AGATGGGAGT TTGCCACCTG 
     3841 TTCTTAATGA TGTGTGGACT AGAGAGAAGG AAGCAGCTAA GGAAACTGAG TTGGAAGATA 
     3901 AGGTTGCTGT GCAGCAGAGT GAAGTTTGTG AAGATAGAAT TCCAGGGAAC GTGGACCAAT 
     3961 CCTGTAAGGA TCAGAGAGAT CCTGCAGTAG ACGATTCTCC GCAGTCTGGC TGTGATGTAG 
     4021 AGAAGTCAGT ACAGCCAGAA TCGATTTTCC AGAAAGTGGT TCATTCTAAG GACTTGAACT 
     4081 TAGTTCAGGC AGTTCATTGC TCACCAGAAG AACCAATTCC AATTCGATCT CACTCTGATT 
     4141 CTCCACCAAA AACTAAGAGC AAGAATTCCT TACTGATTGG ACTTTCAACT GGTCTGTTTG 
     4201 ATGCAAACAA TCCAAAGATG CTGAGGACCT GCTCACTTCC AGATCTTTCC AAGCTGTTCA 
     4261 GAACCCTAAT GGACGTTCCC ACTGTGGGGG ACGTTCATCA AGACAGTCTT GAAATCGATG 
     4321 AGCTGGAAGA TGAACCAATT AAAGAAGGGC CTTCTGATTC CGAAGACACT GTATTTGAAG 
     4381 AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCAATGGA GCAGCTGCTT AGGGAGCAAC 
     4441 CAGGTGACGA ATACAGTGAG GAGGAAGAGT CTGTTTTAAA AAGCAGCGAT GTGGAGCAGA 
     4501 CAGCAAGAGG GACAGATGCC CCAGACGAGG AGGACAACCC CAGCAGCGAA AGCGCCCTGA 
     4561 ACGAGGAATG GCACTCAGAT AATAGTGACG CTGAGACCAC TAGTGAATGT GAATATGACA 
     4621 GTGTCTTTAA CCATTTAGAG GAACTAAGAC TTCACTTGGA GCAAGAAATG GGCTTTGAAA 
     4681 AGTTCTTTGA GGTTTATGAG AAAGTAAAGG CTATTCATGA GGATGAAGAT GAAAATATTG 
     4741 AAATTTGTTC AACAATAGTT GAGAATATTT TGGGCAATGA GCACCAGCAT CTCTATGCCA 
     4801 AGATTCTGCA TTTAGTCATG GCAGATGGAG CCTATCAGGA AGATAATGAT GAAACGCGTA 
     4861 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4921 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4981 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5041 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5101 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5161 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5221 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5281 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5341 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5401 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5461 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5521 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5581 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5641 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5701 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5761 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5821 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5881 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5941 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6001 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6061 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6121 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6181 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6241 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6301 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6361 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6421 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6481 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6541 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6601 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6661 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6721 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6781 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6841 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6901 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6961 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7021 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7081 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7141 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7201 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7261 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7321 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7381 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7441 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7501 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7561 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7621 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7681 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7741 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7801 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7861 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7921 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8161 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8221 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8281 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8341 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8401 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8461 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8521 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8581 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8641 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8701 AATTTT
//