LOCUS       MR231745                8691 bp    DNA     circular  SYN 
DEFINITION  Flii (myc-DDK-tagged) - Mouse flightless I homolog (Drosophila) (Flii), transcript variant 2
ACCESSION   NM_001302207
VERSION     NM_001302207.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4848..4939 
                     /label="Myc-DDK"  
     misc_feature    4969..5554 
                     /label="PolyA signal"  
     misc_feature    5026..5045 
                     /label="Restriction Enzyme"  
     misc_feature    5830..6502 
                     /label="ColE1"  
     misc_feature    6932..7726 
                     /label="Kan/Neo"  
     misc_feature    7761..8063 
                     /label="SV40 ori"  
     misc_feature    8149..8626 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4830..4836 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4830 
                     /label="Flii"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCACC GGGGTGCTGC CGTTCGTGCG CGGCGTGGAC CTCAGCGGCA 
     1081 ACGACTTCAA GGGTGGCTAC TTCCCTGAGA ATGTTAAGGC TATGACCAGC CTGCGATGGC 
     1141 TAAAGCTGAA TCGCACAGGC CTCTGCTACC TGCCTGAAGA ACTTGCGGCT CTGCAGAAGC 
     1201 TGGAGCACCT GTCTGTGAGC CACAACCATC TGACCACGCT TCACGGCGAA CTGTCTAGCC 
     1261 TGCCTTCGCT TCGGGCCATT GTAGCTCGGG CCAACAGCCT GAAGAATTCC GGAGTCCCTG 
     1321 ATGACATCTT CAAGCTGGAT GATCTCTCTG TTCTGGACTT GAGCCATAAC CAGTTGACAG 
     1381 AATGCCCACG GGAGCTGGAG AATGCCAAGA ACATGCTGGT GCTCAACCTC AGCCACAACG 
     1441 GCATCGACTC CATCCCCAAC CAGCTGTTCA TCAACCTTAC GGACTTGCTG TACCTGGACC 
     1501 TCAGTGAAAA CCGCCTAGAG AGCCTTCCCC CTCAAATGCG CCGCCTCGTG CACCTTCAGA 
     1561 CACTGGTGCT GAATGGAAAC CCGCTGCTAC ATGCACAGCT CCGGCAGCTC CCAGCCATGA 
     1621 TGGCCCTACA GACCCTGCAT CTGAGGAACA CTCAACGCAC CCAGAGCAAC CTCCCCACCA 
     1681 GCCTGGAGGG CCTGAGCAAC CTTTCAGATG TGGACCTGTC GTGCAATGAC TTGACAAGGG 
     1741 TGCCGGAGTG CCTGTACACC CTCCCCAGCC TGCGCCGCCT CAACCTCAGT AGCAACCAGA 
     1801 TTGCAGAGCT GTCCCTGTGC ATCGACCAGT GGGTGCACCT GGAGACCTTG AATCTGTCCC 
     1861 GGAACCAGCT CACCTCCCTG CCTTCAGCCA TCTGCAAGCT CACCAAGCTG AAGAAGCTGT 
     1921 ACCTCAACTC CAACAAGCTG GACTTTGATG GGCTGCCTTC TGGCATCGGC AAGCTCACCA 
     1981 GCCTGGAGGA ATTCATGGCT GCCAACAACA ACCTAGAACT GATTCCTGAA AGTCTCTGCA 
     2041 GGTGCCCAAA ACTGAAGAAA CTCGTCCTGA ATAAGAACCG CCTGGTGACC CTTCCTGAGG 
     2101 CCATCCACTT CCTGACAGAG ATCCAGGTCC TGGATGTTCG AGAAAACCCC AGCTTGGTCA 
     2161 TGCCGCCCAA GCCTGCTGAC CGCACCGCCG AGTGGTACAA CATTGACTTC TCGCTACAGA 
     2221 ACCAGCTGAG GCTGGCGGGC GCCTCCCCGG CCACAGTGGC TGCTGCTGCA GCTGGGAGCG 
     2281 GGTCCAAGGA CCCCTTGGCT CGCAAGATGC GGCTACGGAG GCGCAAGGAC TCAGCACAGG 
     2341 ATGTCCAGGC CAAGCAAGTG CTAAAGGGCA TGTCAGATGT TGCCCAGGAG AAAAACAAAA 
     2401 ACCAAGAGGA AAGCATAGAT GCCCGGGCAC CAGGGGGAAA GGTACGACGC TGGGACCAGG 
     2461 GCCTGGAGAA GCCACGCCTT GACTACTCGG AGTTCTTTAC GGAGGATGTG GGCCAGCTGC 
     2521 CTGGTTTGAC CATCTGGCAA ATTGAGAACT TTGTGCCTGT TCTGGTGGAA GAAGCCTTCC 
     2581 ATGGCAAGTT CTACGAGGCT GACTGCTACA TTGTGCTCAA GACCTTCCTG GATGACAGCG 
     2641 GCTCCCTGAA CTGGGAGATC TACTACTGGA TTGGTGGGGA GGCCACACTT GACAAGAAAG 
     2701 CCTGTTCCGC CATTCATGCA GTCAATCTGC GCAACTACCT GGGTGCTGAG TGCCGTACTG 
     2761 TTCGAGAGGA GATGGGTGAC GAGAGTGAGG AGTTCCTGCA GGTGTTTGAC AATGACATCT 
     2821 CCTACATTGA AGGCGGAACA GCCAGTGGCT TCTATACTGT GGAAGATACA CACTATGTTA 
     2881 CCAGGATGTA CCGTGTATAT GGGAAAAAGA ACATCAAATT GGAGCCTGTG CCCCTCAAAG 
     2941 GGTCCTCACT AGATCCAAGG TTTGTGTTTC TCCTGGACCA AGGGCTGGAC ATTTATGTGT 
     3001 GGCGGGGGGC CCAGGCCACA CTGAGTAACA CGACTAAGGC CAGGCTCTTT GCAGAGAAAA 
     3061 TAAACAAGAA TGAGCGGAAA GGGAAGGCGG AAATCACACT CCTGGTGCAG GGCCAAGAGC 
     3121 CCCCAGGGTT CTGGGATGTG CTGGGTGGAG AGCCTTCTGA GATCAAGAAT CATGTGCCTG 
     3181 ATGACTTTTG GCCACCCCAG CCCAAGCTAT ACAAGGTGGG CCTGGGTCTG GGCTACTTGG 
     3241 AGCTTCCGCA GATCAACTAC AAGCTCTCAG TGGAACACAA AAAACGGCCC AAGGTGGAGC 
     3301 TGATGCCAGG AATGAGGCTG CTGCAGAGCC TACTGGACAC ACGATGCGTG TACATCCTGG 
     3361 ACTGCTGGTC TGATGTATTC ATCTGGCTGG GCCGCAAGTC CCCACGCCTA GTGCGTGCAG 
     3421 CAGCACTCAA ACTTGGCCAG GAGCTATGTG GGATGCTACA CCGGCCACGA CATACAGTGG 
     3481 TCAGCCGCAG CCTGGAGGGC ACCGAGGCCC AGGTGTTCAA GGCCAAGTTC AAGAACTGGG 
     3541 ACGATGTGTT GACAGTGGAC TACACACGGA ATGCAGAGGC TGTGCTTCAG GGCCAGGGCC 
     3601 TCTCTGGGAA GGTGAAGCGT GACACTGAGA AGACAGACCA GATGAAGGCT GACCTCACTG 
     3661 CACTCTTCCT ACCTCGGCAG CCCCCCATGC CCCTGGCTGA GGCAGAGCAG CTGATGGAGG 
     3721 AGTGGAATGA GGACCTGGAT GGCATGGAGG GCTTCGTGCT GGAGGGCCGC AAGTTCACTC 
     3781 GACTGCCAGA GGAGGAGTTT GGCCACTTCT ACACACAGGA CTGCTATGTC TTCCTCTGCA 
     3841 GGTACTGGGT ACCTGTGGAG TACGAGGAGG AAGAGAAGAC AGAAGACAAG GAGGGGAAGG 
     3901 CCTCGGCAGA GGCCAGAGAA GGGGAGGAAG CAGCAGCGGA GGCAGAGGAG AAGCAGCCAG 
     3961 AGGAGGACTT TCAGTGCATC GTTTACTTCT GGCAGGGCCG GGAGGCCTCC AACATGGGCT 
     4021 GGCTGACCTT CACATTCAGT CTTCAGAAGA AGTTTGAGAG CCTCTTCCCT GGCAAGCTGG 
     4081 AGGTAGTACG TATGACACAG CAGCAGGAGA ACCCCAAGTT CCTATCCCAT TTCAAAAGAA 
     4141 AGTTCATCAT TCACCGAGGC AAGAGGAAGG TGACCCAGGG TACTCTTCAG CCGACCCTCT 
     4201 ATCAGATCCG CACCAACGGC AGTGCCCTCT GCACCCGGTG CATCCAGATT AACACTGACT 
     4261 CCAGCCTTCT CAACTCTGAG TTCTGCTTCA TCCTCAAGGT CCCCTTTGAA AGCGAGGACA 
     4321 ACCAAGGCAT TGTGTATGCC TGGGTAGGTC GGGCATCAGA CCCCGACGAA GCCAAGCTGG 
     4381 CAGAGGACAT CCTGAACACT ATGTTTGATG CATCCTACAG CAAACAGGTC ATCAATGAAG 
     4441 GCGAGGAGCC AGAGAACTTC TTCTGGGTAG GCATTGGTGC ACAGAAACCC TATGATGACG 
     4501 ATGCGGAGTA CATGAAGCAC ACGAGGCTCT TCAGGTGCTC CAATGAGAAG GGTTACTTCG 
     4561 CAGTGACTGA GAAATGCTCT GACTTTTGCC AAGATGACCT GGCAGACGAT GACATCATGC 
     4621 TGCTAGACAA TGGCCAAGAA GTCTACATGT GGGTTGGGAC CCAGACAAGC CAAGTGGAGA 
     4681 TCAAACTGAG TCTGAAGGCT TGCCAGGTAT ACATCCAGCA CACACGCTCT AAAGAACATG 
     4741 AGCGGCCACG TCGCCTGCGC CTGGTCCGCA AAGGTAATGA GCAGCGCGCC TTCACCCGCT 
     4801 GCTTCCACGC CTGGAGCACG TTCCGCCAAG CCCCAGCCAC GCGTACGCGG CCGCTCGAGC 
     4861 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4921 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4981 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5041 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5101 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5161 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5221 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5281 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5341 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5401 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5461 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5521 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5581 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5641 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5701 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5761 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5821 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5881 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5941 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6001 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6061 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6121 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6181 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6241 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6301 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6361 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6421 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6481 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6541 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6601 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6661 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6721 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6781 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6841 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6901 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6961 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7021 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7081 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7141 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7201 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7261 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7321 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7381 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7441 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7501 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7561 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7621 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7681 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7741 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7801 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7861 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7921 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8041 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8101 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8161 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8221 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8281 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8341 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8401 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8461 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8521 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8581 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8641 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//