LOCUS       MR231743                8685 bp    DNA     circular  SYN 
DEFINITION  Stag2 (myc-DDK-tagged) - Mouse stromal antigen 2 (Stag2), transcript variant 3
ACCESSION   NM_001290713
VERSION     NM_001290713.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4842..4933 
                     /label="Myc-DDK"  
     misc_feature    4963..5548 
                     /label="PolyA signal"  
     misc_feature    5020..5039 
                     /label="Restriction Enzyme"  
     misc_feature    5824..6496 
                     /label="ColE1"  
     misc_feature    6926..7720 
                     /label="Kan/Neo"  
     misc_feature    7755..8057 
                     /label="SV40 ori"  
     misc_feature    8143..8620 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4824..4830 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4824 
                     /label="Stag2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGCAGCT CCAGAAATAC CAACTGATTT TAATCTACTG CAGGAGTCAG 
     1081 AGACACACTT TTCTTCTGAC ACAGATTTTG AAGACATTGA AGGAAAAAAT CAGAAGCAAG 
     1141 GCAAAGGCAA AACTTGTAAA AAAGGCAAAA AGGGTCCAGC AGAAAAGGGT AAAAGTGGAA 
     1201 ATGGAGGAGG AAAACCTCCT TCTGGCTCAA ACCGAATGAA CGGTCATCAT CAACAGAATG 
     1261 GAGTTGAAAA CATGATGTTG TTCGAAGTTG TTAAAATGGG CAAGAGTGCT ATGCAGTCGG 
     1321 TGGTAGATGA CTGGATCGAG TCCTACAAGC ATGACCGGGA CATAGCGCTT CTTGACCTTA 
     1381 TCAATTTCTT TATTCAGTGC TCAGGCTGCA AAGGAGTCGT CACAGCAGAG ATGTTCAGAC 
     1441 ATATGCAGAA CTCTGAGATA ATTCGAAAAA TGACAGAAGA ATTTGACGAG GATAGTGGGG 
     1501 ATTACCCACT TACCATGGCT GGTCCTCAGT GGAAGAAGTT CAAGTCCAGC TTCTGTGAGT 
     1561 TCATTGGTGT GTTAGTACGG CAGTGTCAAT ACAGTATCAT ATATGATGAG TACATGATGG 
     1621 ATACTGTCAT TTCACTTCTT ACTGGATTGT CTGACTCCCA AGTCAGAGCA TTTCGACATA 
     1681 CAAGCACCCT GGCAGCTATG AAGTTGATGA CTGCTTTGGT AAATGTGGCA CTAAATCTTA 
     1741 GCATTAATAT GGATAACACA CAAAGACAGT ACGAGGCAGA ACGGAATAAA ATGATTGGAA 
     1801 AACGAGCTAA CGAAAGGCTA GAACTCCTGT TACAAAAGCG TAAAGAGCTT CAGGAAAATC 
     1861 AAGATGAGAT AGAAAATATG ATGAATGCAA TATTTAAAGG AGTGTTTGTA CATAGATACA 
     1921 GGGATGCAAT AGCTGAAATC AGAGCTATAT GCATTGAAGA GATTGGCATT TGGATGAAGA 
     1981 TGTATAGTGA TGCCTTCCTT AATGACAGCT ATTTAAAATA TGTTGGTTGG ACTATGCATG 
     2041 ATAAGCAAGG TGAAGTCAGA CTCAAATGTC TTACTGCTTT ACAAGGGCTT TACTATAACA 
     2101 AAGAACTTAA TTCCAAACTG GAACTCTTTA CCAGTCGATT CAAGGATAGA ATTGTGTCTA 
     2161 TGACCCTTGA CAAAGAATAT GATGTTGCAG TACAAGCAAT AAAGTTACTC ACTCTTGTTT 
     2221 TACAGAGTAG TGAAGAAGTT CTAACTGCAG AAGATTGTGA AAATGTCTAT CATCTGGTTT 
     2281 ATTCAGCTCA CCGGCCAGTA GCAGTTGCAG CTGGAGAATT TCTCTACAAA AAGCTCTTCA 
     2341 GCCGCAGAGA CCCAGAAGAG GATGGGCTGA TGAAAAGAAG GGGAAGGCAA GGCCCGAATG 
     2401 CCAACCTTGT GAAGACTTTG GTGTTTTTCT TTTTGGAAAG TGAGTTACAC GAGCATGCAG 
     2461 CTTACCTTGT GGACAGCATG TGGGATTGTG CTACTGAGCT CCTGAAAGAC TGGGAATGTA 
     2521 TGAATAGCTT GTTATTAGAA GAGCCTCTTA GTGGAGAGGA AGCTCTAACA GACAGACAAG 
     2581 AGAGTGCTTT GATTGAAATA ATGCTTTGTA CTATTAGACA AGCAGCTGAA TGTCATCCTC 
     2641 CCGTGGGAAG AGGGACAGGA AAAAGGGTGC TGACAGCAAA GGAGAAGAAG ACACAGTTGG 
     2701 ATGACAGAAC AAGAATCACT GAGCTGTTTG CTGTGGCCCT TCCGCAGTTA TTAGCTAAAT 
     2761 ACTCTGTAGA TGCAGAAAAG GTGACCAATT TGTTGCAGTT ACCTCAGTAC TTTGATTTGG 
     2821 AGATATACAC CACTGGACGA TTAGAAAAGC ATTTGGATGC CTTATTGCGC CAGATCCGAA 
     2881 ATATTGTAGA GAAGCACACA GACACAGATG TTTTGGAAGC ATGTTCTAAA ACTTACCATG 
     2941 CATTATGTAA CGAAGAGTTC ACCATCTTCA ACAGAGTAGA TATTTCAAGA AGTCAGCTGA 
     3001 TTGATGAACT GGCAGATAAA TTTAACCGGC TTCTTGAAGA TTTTCTGCAA GAGGGAGAAG 
     3061 AACCTGATGA AGATGACGCA TATCAGGTAT TGTCAACATT GAAGAGGATC ACTGCTTTTC 
     3121 ATAATGCTCA TGATCTTTCA AAGTGGGATT TGTTCGCTTG TAATTACAAA CTCTTGAAAA 
     3181 CCGGAATCGA AAATGGAGAT ATGCCTGAGC AGATTGTTAT TCACGCACTG CAGTGTGCTC 
     3241 ACTATGTAAT CCTTTGGCAG CTTGCTAAGA TCACTGAAAG CACGTCTACA AAGGAGGACT 
     3301 TACTGCGCTT AAAGAAACAG ATGAGGGTAT TTTGCCAGAT ATGTCAGCAT TACTTGACCA 
     3361 ACGTGAATAC TACTGTTAAA GAACAGGCCT TCACTATTCT GTGTGACATT TTGATGATCT 
     3421 TCAGTCACCA GATTATGTCT GGAGGGCGTG ACATGTTAGA GCCATTAGTT TATACTCCTG 
     3481 ATTCTTCATT GCAGTCTGAG TTGCTCAGCT TTATTTTGGA TCATGTCTTC ATTGAACAGG 
     3541 ATGATGATAG TAATAGTGCA GATGGTCAGC AAGAGGATGA AGCCAGCAAA ATTGAAGCTT 
     3601 TGCACAAGAG GAGAAATTTA CTTGCAGCAT TTTGTAAGCT AATTGTATAT ACCGTGGTGG 
     3661 AGATGAACAC AGCTGCAGAT ATCTTCAAGC AGTATATGAA GTATTATAAT GATTATGGCG 
     3721 ATATCATCAA AGAAACAATG AGTAAAACAA GGCAGATTGA CAAAATTCAG TGTGCTAAGA 
     3781 CCCTTATTCT CAGTTTGCAG CAGCTTTTTA ATGAGATGAT ACAAGAGAAT GGCTATAATT 
     3841 TTGATAGATC ATCCTCAACA TTTAGTGGCA TAAAAGAACT TGCTCGGCGT TTTGCTTTAA 
     3901 CTTTTGGACT TGATCAATTG AAAACCAGAG AAGCCATTGC CATGCTCCAC AAAGATGGTA 
     3961 TAGAATTTGC TTTTAAGGAG CCTAATCCAC AAGGGGAGAG CCATCCACCT TTAAACTTGG 
     4021 CATTTCTTGA TATTCTCAGT GAATTTTCTT CTAAACTGCT CCGACAGGAT AAAAGAACAG 
     4081 TGTATGTTTA TTTGGAAAAG TTCATGACCT TTCAGATGTC ACTCCGAAGA GAAGATGTAT 
     4141 GGCTTCCTCT GATGTCTTAC CGAAATTCTT TGCTAGCTGG TGGTGATGAT GATACTATGT 
     4201 CAGTCATTAG TGGAATGAGC AGTCGGGGAT CAACTGTACG GAGTAAAAAA TCAAAACCAT 
     4261 CTACAGGAAA GCGGAAAGTG GTTGAAGGCA TGCAACTAGC ACTCCCTGAA GAAAGCAGTA 
     4321 GCAGTGACAG TATGTGGTTG AGCAGAGAGC AAACACTGCA CACCCCTGTC ATGATGCAGA 
     4381 CACCACAGCT CACCTCCACT ATTATGAGAG AACCGAAAAG GTTACGGCCT GAAGATAGCT 
     4441 TCATGAGTGT CTATCCGATG CAGGCAGAAC ACCATCAAAC GCCTCTGGAT TATAACACGC 
     4501 AGGTAACATG GATGTTAGCT CAAAGACAAC AAGAGGAAGC AAGGCAACAG CAGGAGAGAG 
     4561 CAGCAATGAG CTATGTTAAA CTGCGAACTA ATCTTCAGCA TGCCATTCGG CGTGGTACAA 
     4621 GCCTAATGGA AGATGATGAA GAGCCAATTG TTGAAGATGT TATGATGTCC TCAGAAGGAA 
     4681 GGATTGAGGA TCTTAATGAG GGAATGGACT TTGACACCAT GGATATAGAC TTGCCACCAT 
     4741 CAAAGAATAG AAGAGAGAGA ACAGAACTGA AGCCCGATTT CTTTGATCCA GCTTCAATTA 
     4801 TGGATGAATC AGTTCTTGGG GTGTCAATGT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4861 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4921 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4981 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5041 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5101 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5161 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5221 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5281 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5341 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5401 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5461 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5521 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5581 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5641 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5701 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5761 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5821 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5881 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5941 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6001 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6061 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6121 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6181 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6241 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6301 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6361 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6421 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6481 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6541 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6601 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6661 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6721 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6781 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6841 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6901 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6961 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7021 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7081 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7141 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7201 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7261 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7321 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7381 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7441 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7501 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7561 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7621 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7681 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7741 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7801 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7861 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7921 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8101 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8161 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8221 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8281 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8341 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8401 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8461 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8521 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8581 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8641 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//