LOCUS       MR231736                8670 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 2
ACCESSION   NM_001253359
VERSION     NM_001253359.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4825..4916 
                     /label="Myc-DDK"  
     misc_feature    4946..5531 
                     /label="PolyA signal"  
     misc_feature    5003..5022 
                     /label="Restriction Enzyme"  
     misc_feature    5807..6479 
                     /label="ColE1"  
     misc_feature    6909..7703 
                     /label="Kan/Neo"  
     misc_feature    7738..8040 
                     /label="SV40 ori"  
     misc_feature    8126..8603 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4800..4807 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4800 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT AAACAATGGC TCCAGCCAGG 
     1441 CAGATGGTAC TCTCAAGCCA GTGGACGAAA AAGAGGAGGT GGTGGCAGCC GAGGTCGGCT 
     1501 GGATGACATC TGTGAAGGAC TGGGCAGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTGTTAGTC TTTGCTCTCA GCATTGGTGC CCTCGTAATA TACTTCATAG 
     1621 ACTCGTCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTCCTCT ACTTTGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAGCTGTG GTTCTGGCTG GAAGTGAATT CAGTAGTAGA TTTCTTCACA GTCCCTCCTG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGCTT GAGATTTTTA AGAGCTCTCA 
     1861 GACTGATACA GTTTTCAGAG ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCTGGATTCA 
     1981 TCCACTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCACTTA 
     2041 CGTACTGGGA ATGTGTCTAC TTACTCATGG TCACAATGTC TACAGTGGGT TATGGGGACG 
     2101 TTTATGCAAA AACCACACTT GGACGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGCGCGGTT AGTGGAAGAA AGCACATTGT AGTCTGTGGA CACATTACTC 
     2281 TGGAGAGTGT CTCTAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GATGTCAACG 
     2341 TGGAGATTGT CTTTCTTCAC AACATCTCCC CTAACCTTGA GCTTGAGGCT CTGTTCAAAC 
     2401 GGCATTTCAC TCAGGTGGAG TTTTATCAGG GCTCTGTCCT CAATCCACAT GATCTTGCCA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAATAAGTAT TGCGCTGACC 
     2521 CGGATGCAGA AGATGCCTCC AACATCATGA GAGTGATCTC CATCAAAAAC TACCACCCAA 
     2581 AGATCAGGAT CATCACTCAG ATGCTGCAGT ATCACAACAA GGCCCATCTG CTCAACATCC 
     2641 CCAGCTGGAA CTGGAAAGAG GGTGATGACG CAATATGCCT TGCAGAGCTC AAGTTGGGTT 
     2701 TCATAGCCCA GAGCTGTCTG GCTCAAGGCC TCTCCACAAT GCTTGCCAAT CTCTTCTCTA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CCAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTTAAGC TCCTGATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACAGAGA GAGCCGAATA TTAATTAACC CTGGGAACCA CCTTAAGATC CAAGAAGGTA 
     3001 CTTTAGGATT TTTCATCGCA AGTGATGCCA AAGAAGTTAA AAGGGCATTT TTTTACTGCA 
     3061 AGGCCTGTCA TGATGACGTC ACAGATCCCA AAAGAATTAA AAAATGTGGC TGCAGGCGGC 
     3121 TGATCTATTT TGAAGATGAG CAGCCGCCAA CCCTGTCACC AAAAAAAAAA CAACGTAATG 
     3181 GGGGCATGAG GAACTCGCCC AACACCTCCC CGAAGCTGAT GAGGCATGAC CCCTTGTTAA 
     3241 TTCCTGGCAA TGATCAGATT GACAACATGG ACTCCAATGT GAAAAAGTAC GACTCCACTG 
     3301 GAATGTTTCA CTGGTGTGCA CCCAAGGAGA TTGAGAAAGT CATCTTGACT CGAAGTGAAG 
     3361 CTGCCATGAC TGTCCTGAGT GGCCATGTCG TAGTCTGCAT CTTTGGGGAT GTCAGCTCAG 
     3421 CCCTGATTGG CCTCCGGAAC CTGGTGATGC CACTTCGTGC TAGCAACTTT CACTATCATG 
     3481 AGCTCAAACA CATTGTGTTT GTGGGCTCCA TTGAGTACCT CAAGAGGGAG TGGGAAACAC 
     3541 TGCACAACTT CCCGAAAGTG TCCATATTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA 
     3601 GGGCTGTCAA CATCAACCTC TGTGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA 
     3661 TTGATGATAC TTCGCTTCAG GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA 
     3721 TGCAGTTTGA TGACAGCATC GGGGTCTTGC AGGCTAATTC CCAAGGATTC ACACCTCCTG 
     3781 GAATGGACAG ATCATCACCC GACAACAGCC CAGTGCACGG GATGTTACGC CAGCCGTCCA 
     3841 TCACAACTGG GGTCAACATT CCCATCATCA CGGAACTCGC TAAGCCGGGC AAGTTGCCTT 
     3901 TGGTATCAGT CAATCAGGAA AAAAACAGTG GGACGCACAT TCTAATGATA ACGGAGTTGG 
     3961 TGAATGATAC CAATGTTCAG TTTTTGGACC AAGACGATGA CGATGACCCT GACACAGAGC 
     4021 TGTACCTCAC ACAGCCCTTT GCTTGTGGGA CAGCATTTGC CGTCAGCGTC CTGGACTCAC 
     4081 TCATGAGCGC GACATACTTC AATGACAATA TCCTCACCCT AATACGGACC CTGGTGACAG 
     4141 GAGGAGCCAC ACCAGAGCTC GAGGCTCTAA TAGCTGAGGA GAATGCACTT CGAGGAGGCT 
     4201 ACAGCACTCC GCAGACATTG GCCAACAGGG ACCGTTGCCG AGTGGCCCAG TTAGCCCTGT 
     4261 TAGATGGTCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATCTG TTCTGCAAAG 
     4321 CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCCCACCTCA 
     4381 GCACCCCCAG CCAGTGTACA AAAAGGTACG TCATCACCAA TCCTCCCTAC GAGTTTGAGC 
     4441 TCGTACCAAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCT GGCCAATCCC 
     4501 GGGCCAGTCT GTCTCATTCC TCCCACTCCT CACAGTCGTC CAGTAAGAAG AGCTCCTCCG 
     4561 TCCACTCCAT CCCGTCCACA GCAAATCGGC CGAACCGGCC CAAGTCCAGG GAGTCCCGCG 
     4621 ACAAACAGAA TGCAACAAGG ATGACTAGAA TGGGCCAAGA AAAGAAATGG TTTACAGATG 
     4681 AGCCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGTACC CACATGGCTA 
     4741 ACCAGATCAA CCAATATAAA TCCACAAGCA GCCTGATCCC TCCAATCAGA GAAGTTGAAG 
     4801 ATGAATGTAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4861 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4921 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4981 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5041 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5101 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5161 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5221 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5281 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5341 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5401 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5461 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5521 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5581 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5641 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5701 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5761 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5821 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5881 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5941 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6001 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6061 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6121 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6181 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6241 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6301 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6361 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6421 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6481 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6541 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6601 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6661 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6721 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6781 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6841 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6901 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6961 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7021 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7081 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7141 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7201 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7261 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7321 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7381 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7441 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7501 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7561 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7621 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7681 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7741 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7801 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7861 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7921 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8041 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8101 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8161 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8221 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8281 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8341 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8401 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8461 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8521 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8581 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8641 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//