LOCUS       MR231729                8628 bp    DNA     circular  SYN 
DEFINITION  Ace (myc-DDK-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), transcript variant 3
ACCESSION   NM_001281819
VERSION     NM_001281819.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Ace"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCCGCG TCCGGCCAGC GGGGGCGGTG GCCGTTGTCG CCTCCGCTCT 
     1081 TGATGCTGTC GCTGCTGGTG CTGTTGCTGC AGCCGTCGCC CGCCCCGGCA CTCGACCCTG 
     1141 GATTGCAGCC GGGCAACTTT TCCCCGGACG AGGCAGGGGC GCAGCTTTTC GCTGAAAGCT 
     1201 ATAACTCGAG TGCCGAGGTG GTGATGTTCC AGAGCACCGT GGCCAGTTGG GCGCACGACA 
     1261 CCAACATCAC GGAGGAGAAC GCGCGACGCC AGGAGGAAGC GGCCCTGGTC AGCCAGGAGT 
     1321 TTGCAGAGGT CTGGGGCAAG AAGGCCAAGG AGTTGTATGA GTCCATTTGG CAGAACTTTA 
     1381 CTGACTCAAA GCTGCGAAGG ATCATCGGAT CTATTCGGAC CCTAGGACCT GCCAATCTGC 
     1441 CCCTGGCCCA GCGGCAGCAG TACAACTCTC TGCTAAGCAA CATGAGCAGA ATCTACTCCA 
     1501 CTGGCAAGGT CTGCTTCCCC AACAAGACTG CCACCTGCTG GTCCCTGGAC CCAGAGCTCA 
     1561 CCAACATCCT GGCTTCCTCA CGAAGCTATG CCAAGTTGTT GTTTGCCTGG GAGGGCTGGC 
     1621 ATGATGCTGT GGGTATCCCA CTGAAACCCC TCTATCAAGA CTTTACTGCC ATCAGTAACG 
     1681 AAGCCTACAG ACAAGACGAC TTCTCAGACA CAGGAGCCTT CTGGCGCTCC TGGTATGAGT 
     1741 CCCCCTCCTT TGAGGAGAGT CTGGAACATA TCTACCACCA ACTAGAGCCC CTCTACCTGA 
     1801 ACCTCCATGC CTACGTCCGC CGCGCACTGC ACCGCCGCTA TGGGGACAAA TACGTCAATC 
     1861 TCAGGGGGCC TATTCCTGCC CATTTGCTGG GAGACATGTG GGCCCAGAGC TGGGAGAACA 
     1921 TCTACGACAT GGTAGTGCCT TTCCCAGACA AACCCAACCT CGATGTCACC AGTACAATGG 
     1981 TACAGAAGGG CTGGAACGCC ACACACATGT TCCGGGTATC AGAGGAATTC TTCACCTCGC 
     2041 TGGGGCTCTC ACCCATGCCT CCTGAGTTCT GGGCGGAGTC AATGCTGGAG AAACCAACGG 
     2101 ATGGACGGGA GGTGGTGTGC CACGCCTCTG CCTGGGACTT CTACAACCGG AAGGACTTCC 
     2161 GGATTAAGCA ATGCACACGG GTCACGATGG AACAGCTGGC CACAGTACAC CACGAGATGG 
     2221 GCCACGTGCA GTACTACCTC CAGTACAAGG ACCTGCACGT CTCTCTGCGT AGAGGTGCCA 
     2281 ACCCTGGCTT CCATGAGGCC ATTGGGGATG TGCTTGCACT CTCCGTCTCT ACCCCTGCAC 
     2341 ATCTGCACAA AATCGGCCTA CTGGACCATG TTACCAATGA CATAGAGAGT GACATCAATT 
     2401 ACCTGCTAAA GATGGCCCTA GAGAAAATCG CCTTCTTGCC CTTTGGCTAC CTGGTGGACC 
     2461 AGTGGCGTTG GGGGGTCTTC AGTGGACGGA CCCCACCCTC TCGCTACAAC TTCGACTGGT 
     2521 GGTATCTTCG AACCAAGTAT CAGGGGATCT GCCCACCAGT TGCCCGGAAT GAAACCCATT 
     2581 TTGATGCTGG AGCCAAGTTT CACATCCCAA ACGTGACACC GTACATCAGG TACTTCGTGA 
     2641 GCTTTGTGCT GCAGTTCCAG TTCCATCAAG CACTGTGCAA GGAGGCAGGC CACCAGGGCC 
     2701 CACTACACCA GTGTGACATC TACCAGTCCG CCCAGGCGGG GGCCAAGCTC AAGCAGGTGT 
     2761 TGCAGGCTGG CTGCTCCAGG CCCTGGCAGG AGGTACTGAA GGACCTGGTA GGCTCAGATG 
     2821 CCCTGGATGC CAAGGCACTG CTGGAGTACT TCCAACCGGT CAGCCAGTGG CTGGAAGAGC 
     2881 AGAATCAGCG GAATGGCGAA GTCCTAGGCT GGCCAGAGAA TCAGTGGCGT CCACCGTTAC 
     2941 CCGACAACTA TCCAGAGGGC ATTGACCTAG AGACTGATGA AGCCAAGGCT GACAGGTTCG 
     3001 TGGAAGAGTA TGACCGGACA GCCCAAGTGT TGTTGAACGA GTACGCAGAG GCCAACTGGC 
     3061 AATATAACAC CAACATTACC ATAGAGGGCA GCAAGATCCT GCTTGAGAAA AGCACGGAGG 
     3121 TATCCAATCA CACCCTGAAA TATGGCACCC GGGCCAAGAC ATTTGATGTG AGCAACTTTC 
     3181 AAAACTCTTC CATCAAGCGG ATCATAAAGA AGCTTCAGAA CCTGGACCGG GCAGTGCTGC 
     3241 CTCCCAAGGA ATTAGAAGAG TACAACCAGA TCCTGCTAGA CATGGAGACA ACTTACAGCT 
     3301 TATCCAACAT TTGCTACACA AATGGCACTT GTATGCCCCT GGAACCTGAT CTAACAAACA 
     3361 TGATGGCCAC ATCCCGGAAA TATGAAGAAT TGCTATGGGC ATGGAAGAGC TGGAGAGACA 
     3421 AGGTGGGGAG AGCCATCCTT CCTTTTTTCC CAAAGTATGT GGAGTTCTCC AACAAGATTG 
     3481 CCAAGCTCAA TGGCTACACG GATGCAGGGG ATTCATGGAG ATCCTTATAC GAGTCTGACA 
     3541 ACCTGGAGCA AGACCTGGAA AAACTGTACC AGGAGCTGCA GCCACTCTAC CTGAACCTGC 
     3601 ATGCCTATGT GCGTCGTTCC CTGCACCGCC ACTATGGGTC CGAGTACATC AACCTGGATG 
     3661 GCCCCATTCC TGCCCATCTG CTAGGGAACA TGTGGGCGCA GACCTGGTCC AACATCTATG 
     3721 ATTTGGTGGC GCCCTTCCCT TCCGCCCCCA ATATAGATGC CACGGAAGCC ATGATAAAGC 
     3781 AGGGATGGAC ACCCAGAAGG ATATTTAAGG AAGCTGACAA TTTCTTTACC TCCCTGGGGC 
     3841 TGTTACCTGT GCCCCCTGAG TTCTGGAACA AGTCGATGTT AGAGAAGCCC ACCGATGGAA 
     3901 GGGAGGTGGT GTGCCATCCC TCAGCCTGGG ACTTCTACAA CGGCAAGGAC TTCAGGATCA 
     3961 AGCAGTGTAC CTCTGTGAAC ATGGAGGACT TGGTGATAGC GCACCACGAA ATGGGCCACA 
     4021 TCCAGTATTT CATGCAGTAC AAAGACTTAC CCGTGACTTT CCGGGAGGGT GCCAACCCTG 
     4081 GTTTTCATGA AGCTATTGGA GATATAATGG CTCTCTCAGT GTCTACCCCC AAGCATCTAT 
     4141 ACAGTCTCAA CCTGCTTAGC ACTGAGGGCA GTGGCTACGA GTATGACATC AACTTTCTAA 
     4201 TGAAGATGGC CCTCGACAAG ATCGCCTTTA TCCCCTTCAG CTACCTCATC GACCAGTGGC 
     4261 GCTGGAGGGT CTTTGATGGA AGCATCACCA AGGAGAACTA TAACCAGGAG TGGTGGAGCC 
     4321 TCAGGCTGAA GTATCAGGGT CTGTGCCCCC CAGTGCCAAG ATCCCAAGGT GACTTTGACC 
     4381 CAGGGTCCAA GTTCCACGTT CCTGCGAACG TGCCATACGT CAGGTACTTT GTCAGCTTCA 
     4441 TCATCCAGTT CCAGTTCCAC GAGGCGCTGT GTCGCGCAGC CGGGCACACG GGTCCCCTGC 
     4501 ACAAGTGTGA CATCTACCAA TCCAAGGAAG CAGGGAAGCT CCTGGCGGAT GCCATGAAGC 
     4561 TGGGCTACAG TAAGCCGTGG CCAGAGGCCA TGAAGCTGAT CACAGGCCAG CCTAACATGT 
     4621 CAGCCTCCGC CATGATGAAT TACTTCAAGC CACTGACAGA ATGGCTCGTC ACCGAGAACA 
     4681 GGAGACATGG AGAGACACTG GGCTGGCCGG AGTACAACTG GGCGCCAAAC ACCGGTACTA 
     4741 CACCCACCCT GCCTCCAGCC CCCGGGCCCA GCTCCACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//