LOCUS       MR231725                8619 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 4
ACCESSION   NM_001253361
VERSION     NM_001253361.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4774..4865 
                     /label="Myc-DDK"  
     misc_feature    4895..5480 
                     /label="PolyA signal"  
     misc_feature    4952..4971 
                     /label="Restriction Enzyme"  
     misc_feature    5756..6428 
                     /label="ColE1"  
     misc_feature    6858..7652 
                     /label="Kan/Neo"  
     misc_feature    7687..7989 
                     /label="SV40 ori"  
     misc_feature    8075..8552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT AAACAATGGC TCCAGCCAGG 
     1441 CAGATGGTAC TCTCAAGCCA GTGGACGAAA AAGAGGAGGT GGTGGCAGCC GAGGTCGGCT 
     1501 GGATGACATC TGTGAAGGAC TGGGCAGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTGTTAGTC TTTGCTCTCA GCATTGGTGC CCTCGTAATA TACTTCATAG 
     1621 ACTCGTCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTCCTCT ACTTTGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAGCTGTG GTTCTGGCTG GAAGTGAATT CAGTAGTAGA TTTCTTCACA GTCCCTCCTG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGCTT GAGATTTTTA AGAGCTCTCA 
     1861 GACTGATACA GTTTTCAGAG ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCTGGATTCA 
     1981 TCCACTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCACTTA 
     2041 CGTACTGGGA ATGTGTCTAC TTACTCATGG TCACAATGTC TACAGTGGGT TATGGGGACG 
     2101 TTTATGCAAA AACCACACTT GGACGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGCGCGGTT AGTGGAAGAA AGCACATTGT AGTCTGTGGA CACATTACTC 
     2281 TGGAGAGTGT CTCTAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GATGTCAACG 
     2341 TGGAGATTGT CTTTCTTCAC AACATCTCCC CTAACCTTGA GCTTGAGGCT CTGTTCAAAC 
     2401 GGCATTTCAC TCAGGTGGAG TTTTATCAGG GCTCTGTCCT CAATCCACAT GATCTTGCCA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAATAAGTAT TGCGCTGACC 
     2521 CGGATGCAGA AGATGCCTCC AACATCATGA GAGTGATCTC CATCAAAAAC TACCACCCAA 
     2581 AGATCAGGAT CATCACTCAG ATGCTGCAGT ATCACAACAA GGCCCATCTG CTCAACATCC 
     2641 CCAGCTGGAA CTGGAAAGAG GGTGATGACG CAATATGCCT TGCAGAGCTC AAGTTGGGTT 
     2701 TCATAGCCCA GAGCTGTCTG GCTCAAGGCC TCTCCACAAT GCTTGCCAAT CTCTTCTCTA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CCAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTTAAGC TCCTGATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACAGAGA GAGCCGAAGC CGAAAGCGAA TATTAATTAA CCCTGGGAAC CACCTTAAGA 
     3001 TCCAAGAAGG TACTTTAGGA TTTTTCATCG CAAGTGATGC CAAAGAAGTT AAAAGGGCAT 
     3061 TTTTTTACTG CAAGGCCTGT CATGATGACG TCACAGATCC CAAAAGAATT AAAAAATGTG 
     3121 GCTGCAGGCG GCTGATCTAT TCCAAGATGT CCATCTACAA GAGAATGAGA CGAGCATGTT 
     3181 GTTTTGATTG CGGACGTTCT GAGCGTGACT GCTCGTGCAT GTCAGGCCGT GTGCGTGGTA 
     3241 ACGTGGACAC CCTTGAGAGA ACCTTCCCGC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA 
     3301 CCAGTTTCCG TGCCTTTGAA GATGAGCAGC CGCCAACCCT GTCACCAAAA AAAAAACAAC 
     3361 GTAATGGGGG CATGAGGAAC TCGCCCAACA CCTCCCCGAA GCTGATGAGG CATGACCCCT 
     3421 TGTTAATTCC TGGCAATGAT CAGATTGACA ACATGGACTC CAATGTGAAA AAGTACGACT 
     3481 CCACTGGAAT GTTTCACTGG TGTGCACCCA AGGAGATTGA GAAAGTCATC TTGACTCGAA 
     3541 GTGAAGCTGC CATGACTGTC CTGAGTGGCC ATGTCGTAGT CTGCATCTTT GGGGATGTCA 
     3601 GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCACT TCGTGCTAGC AACTTTCACT 
     3661 ATCATGAGCT CAAACACATT GTGTTTGTGG GCTCCATTGA GTACCTCAAG AGGGAGTGGG 
     3721 AAACACTGCA CAACTTCCCG AAAGTGTCCA TATTGCCTGG TACACCATTA AGTCGGGCTG 
     3781 ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA GCCAATCAGA 
     3841 ATAATATTGA TGATACTTCG CTTCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA 
     3901 AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA GGATTCACAC 
     3961 CTCCTGGAAT GGACAGATCA TCACCCGACA ACAGCCCAGT GCACGGGATG TTACGCCAGC 
     4021 CGTCCATCAC AACTGGGGTC AACATTCCCA TCATCACGGA ACTCGTGAAT GATACCAATG 
     4081 TTCAGTTTTT GGACCAAGAC GATGACGATG ACCCTGACAC AGAGCTGTAC CTCACACAGC 
     4141 CCTTTGCTTG TGGGACAGCA TTTGCCGTCA GCGTCCTGGA CTCACTCATG AGCGCGACAT 
     4201 ACTTCAATGA CAATATCCTC ACCCTAATAC GGACCCTGGT GACAGGAGGA GCCACACCAG 
     4261 AGCTCGAGGC TCTAATAGCT GAGGAGAATG CACTTCGAGG AGGCTACAGC ACTCCGCAGA 
     4321 CATTGGCCAA CAGGGACCGT TGCCGAGTGG CCCAGTTAGC CCTGTTAGAT GGTCCCTTTG 
     4381 CAGACTTAGG GGATGGTGGT TGTTATGGTG ATCTGTTCTG CAAAGCTCTG AAAACATATA 
     4441 ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC CCCAGCCAGT 
     4501 GTACAAAAAG GTACGTCATC ACCAATCCTC CCTACGAGTT TGAGCTCGTA CCAACAGACC 
     4561 TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCTGGCCA ATCCCGGGCC AGTCTGTCTC 
     4621 ATTCCTCCCA CTCCTCACAG TCGTCCAGTA AGAAGAGCTC CTCCGTCCAC TCCATCCCGT 
     4681 CCACAGCAAA TCGGCCGAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA CAGAACAGAA 
     4741 AAGAAATGGT TTACAGAAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4801 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4861 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4921 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4981 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5041 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5101 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5161 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5221 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5281 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5341 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5401 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5461 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5521 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5581 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5641 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5701 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5761 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5821 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5881 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5941 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6001 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6061 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6121 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6181 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6241 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6301 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6361 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6421 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6481 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6541 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6601 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6661 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6721 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6781 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6841 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6901 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6961 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7021 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7081 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7141 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7201 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7261 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7321 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7381 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7441 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7501 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7561 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7621 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7681 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7741 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7801 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7861 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8041 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8101 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8161 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8221 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8281 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8341 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8401 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8461 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8521 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8581 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//