LOCUS       MR231720                8610 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 5
ACCESSION   NM_001253362
VERSION     NM_001253362.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4765..4856 
                     /label="Myc-DDK"  
     misc_feature    4886..5471 
                     /label="PolyA signal"  
     misc_feature    4943..4962 
                     /label="Restriction Enzyme"  
     misc_feature    5747..6419 
                     /label="ColE1"  
     misc_feature    6849..7643 
                     /label="Kan/Neo"  
     misc_feature    7678..7980 
                     /label="SV40 ori"  
     misc_feature    8066..8543 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4747 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT AAACAATGGC TCCAGCCAGG 
     1441 CAGATGGTAC TCTCAAGCCA GTGGACGAAA AAGAGGAGGT GGTGGCAGCC GAGGTCGGCT 
     1501 GGATGACATC TGTGAAGGAC TGGGCAGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTGTTAGTC TTTGCTCTCA GCATTGGTGC CCTCGTAATA TACTTCATAG 
     1621 ACTCGTCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTCCTCT ACTTTGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAGCTGTG GTTCTGGCTG GAAGTGAATT CAGTAGTAGA TTTCTTCACA GTCCCTCCTG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGCTT GAGATTTTTA AGAGCTCTCA 
     1861 GACTGATACA GTTTTCAGAG ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCTGGATTCA 
     1981 TCCACTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCACTTA 
     2041 CGTACTGGGA ATGTGTCTAC TTACTCATGG TCACAATGTC TACAGTGGGT TATGGGGACG 
     2101 TTTATGCAAA AACCACACTT GGACGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGCGCGGTT AGTGGAAGAA AGCACATTGT AGTCTGTGGA CACATTACTC 
     2281 TGGAGAGTGT CTCTAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GATGTCAACG 
     2341 TGGAGATTGT CTTTCTTCAC AACATCTCCC CTAACCTTGA GCTTGAGGCT CTGTTCAAAC 
     2401 GGCATTTCAC TCAGGTGGAG TTTTATCAGG GCTCTGTCCT CAATCCACAT GATCTTGCCA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAATAAGTAT TGCGCTGACC 
     2521 CGGATGCAGA AGATGCCTCC AACATCATGA GAGTGATCTC CATCAAAAAC TACCACCCAA 
     2581 AGATCAGGAT CATCACTCAG ATGCTGCAGT ATCACAACAA GGCCCATCTG CTCAACATCC 
     2641 CCAGCTGGAA CTGGAAAGAG GGTGATGACG CAATATGCCT TGCAGAGCTC AAGTTGGGTT 
     2701 TCATAGCCCA GAGCTGTCTG GCTCAAGGCC TCTCCACAAT GCTTGCCAAT CTCTTCTCTA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CCAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTTAAGC TCCTGATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACAGAGA GAGCCGAAGC CGAAAGCGAA TATTAATTAA CCCTGGGAAC CACCTTAAGA 
     3001 TCCAAGAAGG TACTTTAGGA TTTTTCATCG CAAGTGATGC CAAAGAAGTT AAAAGGGCAT 
     3061 TTTTTTACTG CAAGGCCTGT CATGATGACG TCACAGATCC CAAAAGAATT AAAAAATGTG 
     3121 GCTGCAGGCG GCCCAAGATG TCCATCTACA AGAGAATGAG ACGAGCATGT TGTTTTGATT 
     3181 GCGGACGTTC TGAGCGTGAC TGCTCGTGCA TGTCAGGCCG TGTGCGTGGT AACGTGGACA 
     3241 CCCTTGAGAG AACCTTCCCG CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTCC 
     3301 GTGCCTTTGA AGATGAGCAG CCGCCAACCC TGTCACCAAA AAAAAAACAA CGTAATGGGG 
     3361 GCATGAGGAA CTCGCCCAAC ACCTCCCCGA AGCTGATGAG GCATGACCCC TTGTTAATTC 
     3421 CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA AAAGTACGAC TCCACTGGAA 
     3481 TGTTTCACTG GTGTGCACCC AAGGAGATTG AGAAAGTCAT CTTGACTCGA AGTGAAGCTG 
     3541 CCATGACTGT CCTGAGTGGC CATGTCGTAG TCTGCATCTT TGGGGATGTC AGCTCAGCCC 
     3601 TGATTGGCCT CCGGAACCTG GTGATGCCAC TTCGTGCTAG CAACTTTCAC TATCATGAGC 
     3661 TCAAACACAT TGTGTTTGTG GGCTCCATTG AGTACCTCAA GAGGGAGTGG GAAACACTGC 
     3721 ACAACTTCCC GAAAGTGTCC ATATTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG 
     3781 CTGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG 
     3841 ATGATACTTC GCTTCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC 
     3901 AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGATTCACA CCTCCTGGAA 
     3961 TGGACAGATC ATCACCCGAC AACAGCCCAG TGCACGGGAT GTTACGCCAG CCGTCCATCA 
     4021 CAACTGGGGT CAACATTCCC ATCATCACGG AACTCGTGAA TGATACCAAT GTTCAGTTTT 
     4081 TGGACCAAGA CGATGACGAT GACCCTGACA CAGAGCTGTA CCTCACACAG CCCTTTGCTT 
     4141 GTGGGACAGC ATTTGCCGTC AGCGTCCTGG ACTCACTCAT GAGCGCGACA TACTTCAATG 
     4201 ACAATATCCT CACCCTAATA CGGACCCTGG TGACAGGAGG AGCCACACCA GAGCTCGAGG 
     4261 CTCTAATAGC TGAGGAGAAT GCACTTCGAG GAGGCTACAG CACTCCGCAG ACATTGGCCA 
     4321 ACAGGGACCG TTGCCGAGTG GCCCAGTTAG CCCTGTTAGA TGGTCCCTTT GCAGACTTAG 
     4381 GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAT AATATGCTTT 
     4441 GTTTTGGAAT TTACCGGCTG AGAGATGCCC ACCTCAGCAC CCCCAGCCAG TGTACAAAAA 
     4501 GGTACGTCAT CACCAATCCT CCCTACGAGT TTGAGCTCGT ACCAACAGAC CTGATCTTCT 
     4561 GCCTGATGCA GTTTGACCAC AACGCTGGCC AATCCCGGGC CAGTCTGTCT CATTCCTCCC 
     4621 ACTCCTCACA GTCGTCCAGT AAGAAGAGCT CCTCCGTCCA CTCCATCCCG TCCACAGCAA 
     4681 ATCGGCCGAA CCGGCCCAAG TCCAGGGAGT CCCGCGACAA ACAGAACAGA AAAGAAATGG 
     4741 TTTACAGAAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4801 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4861 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4921 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4981 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5041 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5101 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5161 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5221 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5281 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5341 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5401 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5461 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5521 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5581 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5641 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5701 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5761 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5821 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5881 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5941 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6001 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6061 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6121 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6181 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6241 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6301 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6361 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6421 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6481 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6541 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6601 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6661 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6721 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6781 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6841 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6901 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6961 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7021 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7081 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7141 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7201 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7261 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7321 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7381 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7441 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7501 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7561 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7621 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7681 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7741 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7801 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7861 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7981 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8041 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8161 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8221 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8281 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8341 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8401 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8461 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8521 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8581 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//