LOCUS       MR231718                8607 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 6
ACCESSION   NM_001253363
VERSION     NM_001253363.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4762..4853 
                     /label="Myc-DDK"  
     misc_feature    4883..5468 
                     /label="PolyA signal"  
     misc_feature    4940..4959 
                     /label="Restriction Enzyme"  
     misc_feature    5744..6416 
                     /label="ColE1"  
     misc_feature    6846..7640 
                     /label="Kan/Neo"  
     misc_feature    7675..7977 
                     /label="SV40 ori"  
     misc_feature    8063..8540 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4737..4744 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4737 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT AAACAATGGC TCCAGCCAGG 
     1441 CAGATGGTAC TCTCAAGCCA GTGGACGAAA AAGAGGAGGT GGTGGCAGCC GAGGTCGGCT 
     1501 GGATGACATC TGTGAAGGAC TGGGCAGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTGTTAGTC TTTGCTCTCA GCATTGGTGC CCTCGTAATA TACTTCATAG 
     1621 ACTCGTCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTCCTCT ACTTTGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAGCTGTG GTTCTGGCTG GAAGTGAATT CAGTAGTAGA TTTCTTCACA GTCCCTCCTG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGCTT GAGATTTTTA AGAGCTCTCA 
     1861 GACTGATACA GTTTTCAGAG ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCTGGATTCA 
     1981 TCCACTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCACTTA 
     2041 CGTACTGGGA ATGTGTCTAC TTACTCATGG TCACAATGTC TACAGTGGGT TATGGGGACG 
     2101 TTTATGCAAA AACCACACTT GGACGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGCGCGGTT AGTGGAAGAA AGCACATTGT AGTCTGTGGA CACATTACTC 
     2281 TGGAGAGTGT CTCTAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GATGTCAACG 
     2341 TGGAGATTGT CTTTCTTCAC AACATCTCCC CTAACCTTGA GCTTGAGGCT CTGTTCAAAC 
     2401 GGCATTTCAC TCAGGTGGAG TTTTATCAGG GCTCTGTCCT CAATCCACAT GATCTTGCCA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAATAAGTAT TGCGCTGACC 
     2521 CGGATGCAGA AGATGCCTCC AACATCATGA GAGTGATCTC CATCAAAAAC TACCACCCAA 
     2581 AGATCAGGAT CATCACTCAG ATGCTGCAGT ATCACAACAA GGCCCATCTG CTCAACATCC 
     2641 CCAGCTGGAA CTGGAAAGAG GGTGATGACG CAATATGCCT TGCAGAGCTC AAGTTGGGTT 
     2701 TCATAGCCCA GAGCTGTCTG GCTCAAGGCC TCTCCACAAT GCTTGCCAAT CTCTTCTCTA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CCAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTTAAGC TCCTGATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACAGAGA GAGCCGAAGC CGAAAGCGAA TATTAATTAA CCCTGGGAAC CACCTTAAGA 
     3001 TCCAAGAAGG TACTTTAGGA TTTTTCATCG CAAGTGATGC CAAAGAAGTT AAAAGGGCAT 
     3061 TTTTTTACTG CAAGGCCTGT CATGATGACG TCACAGATCC CAAAAGAATT AAAAAATGTG 
     3121 GCTGCAGGCG GCCCAAGATG TCCATCTACA AGAGAATGAG ACGAGCATGT TGTTTTGATT 
     3181 GCGGACGTTC TGAGCGTGAC TGCTCGTGCA TGTCAGGCCG TGTGCGTGGT AACGTGGACA 
     3241 CCCTTGAGAG AACCTTCCCG CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTCC 
     3301 GTGCCTTTGA AGATGAGCAG CCGCCAACCC TGTCACCAAA AAAAAAACAA CGTAATGGGG 
     3361 GCATGAGGAA CTCGCCCAAC ACCTCCCCGA AGCTGATGAG GCATGACCCC TTGTTAATTC 
     3421 CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA AAAGTACGAC TCCACTGGAA 
     3481 TGTTTCACTG GTGTGCACCC AAGGAGATTG AGAAAGTCAT CTTGACTCGA AGTGAAGCTG 
     3541 CCATGACTGT CCTGAGTGGC CATGTCGTAG TCTGCATCTT TGGGGATGTC AGCTCAGCCC 
     3601 TGATTGGCCT CCGGAACCTG GTGATGCCAC TTCGTGCTAG CAACTTTCAC TATCATGAGC 
     3661 TCAAACACAT TGTGTTTGTG GGCTCCATTG AGTACCTCAA GAGGGAGTGG GAAACACTGC 
     3721 ACAACTTCCC GAAAGTGTCC ATATTGCCTG GTACACCATT AAGTCGGGCT GATTTAAGGG 
     3781 CTGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG 
     3841 ATGATACTTC GCTTCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC 
     3901 AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGATTCACA CCTCCTGGAA 
     3961 TGGACAGATC ATCACCCGAC AACAGCCCAG TGCACGGGAT GTTACGCCAG CCGTCCATCA 
     4021 CAACTGGGGT CAACATTCCC ATCATCACGG AACTCGTGAA TGATACCAAT GTTCAGTTTT 
     4081 TGGACCAAGA CGATGACGAT GACCCTGACA CAGAGCTGTA CCTCACACAG CCCTTTGCTT 
     4141 GTGGGACAGC ATTTGCCGTC AGCGTCCTGG ACTCACTCAT GAGCGCGACA TACTTCAATG 
     4201 ACAATATCCT CACCCTAATA CGGACCCTGG TGACAGGAGG AGCCACACCA GAGCTCGAGG 
     4261 CTCTAATAGC TGAGGAGAAT GCACTTCGAG GAGGCTACAG CACTCCGCAG ACATTGGCCA 
     4321 ACAGGGACCG TTGCCGAGTG GCCCAGTTAG CCCTGTTAGA TGGTCCCTTT GCAGACTTAG 
     4381 GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAT AATATGCTTT 
     4441 GTTTTGGAAT TTACCGGCTG AGAGATGCCC ACCTCAGCAC CCCCAGCCAG TGTACAAAAA 
     4501 GGTACGTCAT CACCAATCCT CCCTACGAGT TTGAGCTCGT ACCAACAGAC CTGATCTTCT 
     4561 GCCTGATGCA GTTTGACCAC AACGCTGGCC AATCCCGGGC CAGTCTGTCT CATTCCTCCC 
     4621 ACTCCTCACA GTCGTCCAGT AAGAAGAGCT CCTCCGTCCA CTCCATCCCG TCCACAGCAA 
     4681 ATCGGCCGAA CCGGCCCAAG TCCAGGGAGT CCCGCGACAA ACAGAAAAAA GAAATGGTTT 
     4741 ACAGAAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4801 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4861 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4921 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4981 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5041 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5101 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5161 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5221 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5281 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5341 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5401 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5461 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5521 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5581 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5641 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5701 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5761 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5821 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5881 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5941 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6001 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6061 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6121 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6181 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6241 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6301 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6421 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6481 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6541 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6601 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6661 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6721 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6781 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6841 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6901 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6961 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7021 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7081 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7141 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7201 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7261 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7321 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7381 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7441 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7501 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7561 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7621 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7681 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7741 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7801 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7981 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8041 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8101 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8161 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8221 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8281 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8341 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8401 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8461 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8521 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8581 ATTTAACAAA AATTTAACGC GAATTTT
//