LOCUS       MR231703                8574 bp    DNA     circular  SYN 
DEFINITION  Nek1 (myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 2
ACCESSION   NM_001293638
VERSION     NM_001293638.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="Nek1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTGAGACTGC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CTGTTCTTGT TAAATCGACA GAGGATGGCA GACATTATGT CATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC TGATAAAGAA AGGCAAGAAT CAAGGAGAGA AGTTGCTGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAATATAAAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAA GGAGGTGATT TGTTTAAACG AATAAATGCT CAGAAAGGCG 
     1321 CTCTGTTTCA AGAAGACCAG ATTTTGGACT GGTTTGTGCA GATATGTTTG GCTCTGAAGC 
     1381 ATGTACATGA TAGAAAAATT CTTCACCGAG ACATAAAGTC ACAGAACATA TTTCTAACCA 
     1441 AAGATGGGAC AGTGCAGCTT GGAGATTTTG GAATTGCTCG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGCACTCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AGCCTTATAA CAATAAAAGT GACATTTGGG CTTTGGGCTG TGTCCTTTAT GAGTTGTGTA 
     1621 CACTTAAACA TGCATTTGAA GCTGGAAACA TGAAAAACCT GGTACTGAAG ATAATCTCCG 
     1681 GATCCTTTCC TCCAGTGTCT CCACATTACT CCTATGATCT CCGCAGCTTG CTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCTAAACG AATCGAAAAG TTTCTCTCCC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACACTTTC AAAGTTTGGA CCACAGCCTC TCCCAGGTAA AAGACCAGCA TCAGGACAAG 
     1921 GTGTCAGTTC TTTTGTCCCT GCTCAGAAAA TCACAAAGCC TGCTGCTAAA TACGGAGTGC 
     1981 CTTTAACATA TAAGAAGTAT GGAGATAAAA AGTTACTTGA GAAAAAACCA CCCCCAAAAC 
     2041 ATAAACAGGC CCATCAAATT CCCGTGAAGA AAATGAATTC TGGAGAAGAA AGGAAGAAAA 
     2101 TGTCTGAGGA AGCAGCAAAA AAAAGAAGGT TGGAATTTAT TGAGAAAGAA AAGAAGCAAA 
     2161 AGGATCAGAT TAGGTTCCTG AAGGCTGAGC AGATGAAGCG GCAAGAGAAG CAGCGGTTGG 
     2221 AGAGGATAAA TAGGGCCAGG GAACAAGGAT GGAGGAATGT TTTAAGGGCT GGTGGAAGCG 
     2281 GTGAAGTAAA GGCTTCCTTT TTTGGCATTG GAGGGGCTGT CTCTCCATCA CCGTGTTCTC 
     2341 CTCGAGGCCA GTATGAACAT TACCATGCCA TTTTTGACCA AATGCAGCGG CTAAGAGCAG 
     2401 AAGATAATGA AGCAAGATGG AAGGGGGGAA TCTATGGTCG ATGGCTCCCA GAAAGGCAAA 
     2461 AAGGACACTT AGCTGTAGAG AGAGCCAACC AAGTGGAAGA ATTCCTACAG CGTAAACGAG 
     2521 AAGCTATGCA GAATAAAGCC CGAGCCGAAG GACACGTGGG ACTCCTGCAA AACCTGGCAT 
     2581 CTCTGTATGG AGGCAGGCCC AGCTCTTCAA GAGGAGGGAA GCCAAGGAAC AATGAGGAAG 
     2641 AGGTTTATTT GGCAAGACTG AGGCAAATAA GACTACAAAA TTTTAATGAG CGCCAACAGA 
     2701 TTAAAGCCAA ACTTCGTGGT GAGAATAAAG AAGCTGATGG TACCAAAGGA CAAGAAGCAA 
     2761 CTGAAGAGAC TGACATGAGG CTCAAAAAGA TGGAGTCACT TAAGGCGCAA ACAAATGCAC 
     2821 GTGCTGCTGT ACTAAAAGAA CAGCTGGAGC GAAAAAGAAA GGAAGCTTAT GAAAGAGAAA 
     2881 AGAAAGTATG GGAAGAACAT TTGGTGGCGA GGGTAAAAAG CTCAGATGTT CCTCTGCCTT 
     2941 TGGAACTTCT TGAAACAGGT GGTTCTCCAT CAAAGCAGCA GGTGAAGCCT GTCATTTCTG 
     3001 TGACTTCAGC TTTGAAAGAA GTGGGCCTGG ATGGAAGTTT AACTGATACC CAGGAAGAAG 
     3061 AAATGGAAAA GAGTAACAGT GCTATTTCAA GTAAGCGAGA AATCCTGCGT AGGCTAAATG 
     3121 AAAATCTTAA AGCTCAAGAG GATGAAAAGG AAAAGCAGCA TCACTCAGGT TCTTGTGAGA 
     3181 CCGTTGGTCA CAAAGATGAG AGAGAGTATG AGACAGAAAA TGCCATTTCC TCTGATCGCA 
     3241 AGAAGTGGGA GATGGGAGGT CAGCTTGTGA TTCCTCTCGA TGCAGTGACA CTGGATACAT 
     3301 CCTTCTCTGC AACCGAAAAA CATACTGTGG GAGAGGTTAT TAAATTAGAT TCTAATGGCT 
     3361 CTCCAAGAAA AGTCTGGGGG AAAAACCCTA CAGATTCTGT GCTGAAGATA CTTGGAGAAG 
     3421 CTGAATTACA GCTACAGACA GAACTACTAG AAAACACATC TTTTAAAAGT GAGGTTTATG 
     3481 CTGAAGAGGA GAACTACAAA CCCTTACTTA CTGAAGAAGA GAATCTGCAG TGCATTTCAA 
     3541 AAGAAATAAA TCCATCAGCT ACTGTTGATT CTACTGAAAC GAAAAGTCCA AAGTTTACTG 
     3601 AGGTGTCTCC ACAAATGTCA GAAGGAAATG TGGAAGAACC TGATGATTTG GAAACAGAAG 
     3661 TTCTACAAGA GCCAAGTAGC ACACACACAG ATGGGAGTTT GCCACCTGTT CTTAATGATG 
     3721 TGTGGACTAG AGAGAAGGAA GCAGCTAAGG AAACTGAGTT GGAAGATAAG GTTGCTGTGC 
     3781 AGCAGAGTGA AGTTTGTGAA GATAGAATTC CAGGGAACGT GGACCAATCC TGTAAGGATC 
     3841 AGAGAGATCC TGCAGTAGAC GATTCTCCGC AGTCTGGCTG TGATGTAGAG AAGTCAGTAC 
     3901 AGCCAGAATC GATTTTCCAG AAAGTGGTTC ATTCTAAGGA CTTGAACTTA GTTCAGGCAG 
     3961 TTCATTGCTC ACCAGAAGAA CCAATTCCAA TTCGATCTCA CTCTGATTCT CCACCAAAAA 
     4021 CTAAGAGCAA GAATTCCTTA CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAATC 
     4081 CAAAGATGCT GAGGACCTGC TCACTTCCAG ATCTTTCCAA GCTGTTCAGA ACCCTAATGG 
     4141 ACGTTCCCAC TGTGGGGGAC GTTCATCAAG ACAGTCTTGA AATCGATGAG CTGGAAGATG 
     4201 AACCAATTAA AGAAGGGCCT TCTGATTCCG AAGACACTGT ATTTGAAGAA ACTGACACAG 
     4261 ATTTACAAGA GCTTCAGGCC TCAATGGAGC AGCTGCTTAG GGAGCAACCA GGTGACGAAT 
     4321 ACAGTGAGGA GGAAGAGTCT GTTTTAAAAA GCAGCGATGT GGAGCAGACA GCAAGAGGGA 
     4381 CAGATGCCCC AGACGAGGAG GACAACCCCA GCAGCGAAAG CGCCCTGAAC GAGGAATGGC 
     4441 ACTCAGATAA TAGTGACGCT GAGACCACTA GTGAATGTGA ATATGACAGT GTCTTTAACC 
     4501 ATTTAGAGGA ACTAAGACTT CACTTGGAGC AAGAAATGGG CTTTGAAAAG TTCTTTGAGG 
     4561 TTTATGAGAA AGTAAAGGCT ATTCATGAGG ATGAAGATGA AAATATTGAA ATTTGTTCAA 
     4621 CAATAGTTGA GAATATTTTG GGCAATGAGC ACCAGCATCT CTATGCCAAG ATTCTGCATT 
     4681 TAGTCATGGC AGATGGAGCC TATCAGGAAG ATAATGATGA AACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//