LOCUS       MR231701                8580 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 11
ACCESSION   NM_001253367
VERSION     NM_001253367.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4735..4826 
                     /label="Myc-DDK"  
     misc_feature    4856..5441 
                     /label="PolyA signal"  
     misc_feature    4913..4932 
                     /label="Restriction Enzyme"  
     misc_feature    5717..6389 
                     /label="ColE1"  
     misc_feature    6819..7613 
                     /label="Kan/Neo"  
     misc_feature    7648..7950 
                     /label="SV40 ori"  
     misc_feature    8036..8513 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4717 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT AAACAATGGC TCCAGCCAGG 
     1441 CAGATGGTAC TCTCAAGCCA GTGGACGAAA AAGAGGAGGT GGTGGCAGCC GAGGTCGGCT 
     1501 GGATGACATC TGTGAAGGAC TGGGCAGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTGTTAGTC TTTGCTCTCA GCATTGGTGC CCTCGTAATA TACTTCATAG 
     1621 ACTCGTCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTCCTCT ACTTTGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAGCTGTG GTTCTGGCTG GAAGTGAATT CAGTAGTAGA TTTCTTCACA GTCCCTCCTG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGCTT GAGATTTTTA AGAGCTCTCA 
     1861 GACTGATACA GTTTTCAGAG ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCTGGATTCA 
     1981 TCCACTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCACTTA 
     2041 CGTACTGGGA ATGTGTCTAC TTACTCATGG TCACAATGTC TACAGTGGGT TATGGGGACG 
     2101 TTTATGCAAA AACCACACTT GGACGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGCGCGGTT AGTGGAAGAA AGCACATTGT AGTCTGTGGA CACATTACTC 
     2281 TGGAGAGTGT CTCTAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GATGTCAACG 
     2341 TGGAGATTGT CTTTCTTCAC AACATCTCCC CTAACCTTGA GCTTGAGGCT CTGTTCAAAC 
     2401 GGCATTTCAC TCAGGTGGAG TTTTATCAGG GCTCTGTCCT CAATCCACAT GATCTTGCCA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAATAAGTAT TGCGCTGACC 
     2521 CGGATGCAGA AGATGCCTCC AACATCATGA GAGTGATCTC CATCAAAAAC TACCACCCAA 
     2581 AGATCAGGAT CATCACTCAG ATGCTGCAGT ATCACAACAA GGCCCATCTG CTCAACATCC 
     2641 CCAGCTGGAA CTGGAAAGAG GGTGATGACG CAATATGCCT TGCAGAGCTC AAGTTGGGTT 
     2701 TCATAGCCCA GAGCTGTCTG GCTCAAGGCC TCTCCACAAT GCTTGCCAAT CTCTTCTCTA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CCAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTTAAGC TCCTGATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACAGAGA GAGCCGAATA TTAATTAACC CTGGGAACCA CCTTAAGATC CAAGAAGGTA 
     3001 CTTTAGGATT TTTCATCGCA AGTGATGCCA AAGAAGTTAA AAGGGCATTT TTTTACTGCA 
     3061 AGGCCTGTCA TGATGACGTC ACAGATCCCA AAAGAATTAA AAAATGTGGC TGCAGGCGGC 
     3121 TTGAAGATGA GCAGCCGCCA ACCCTGTCAC CAAAAAAAAA ACAACGTAAT GGGGGCATGA 
     3181 GGAACTCGCC CAACACCTCC CCGAAGCTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA 
     3241 ATGATCAGAT TGACAACATG GACTCCAATG TGAAAAAGTA CGACTCCACT GGAATGTTTC 
     3301 ACTGGTGTGC ACCCAAGGAG ATTGAGAAAG TCATCTTGAC TCGAAGTGAA GCTGCCATGA 
     3361 CTGTCCTGAG TGGCCATGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG 
     3421 GCCTCCGGAA CCTGGTGATG CCACTTCGTG CTAGCAACTT TCACTATCAT GAGCTCAAAC 
     3481 ACATTGTGTT TGTGGGCTCC ATTGAGTACC TCAAGAGGGA GTGGGAAACA CTGCACAACT 
     3541 TCCCGAAAGT GTCCATATTG CCTGGTACAC CATTAAGTCG GGCTGATTTA AGGGCTGTCA 
     3601 ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA 
     3661 CTTCGCTTCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG 
     3721 ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCT GGAATGGACA 
     3781 GATCATCACC CGACAACAGC CCAGTGCACG GGATGTTACG CCAGCCGTCC ATCACAACTG 
     3841 GGGTCAACAT TCCCATCATC ACGGAACTCG TGAATGATAC CAATGTTCAG TTTTTGGACC 
     3901 AAGACGATGA CGATGACCCT GACACAGAGC TGTACCTCAC ACAGCCCTTT GCTTGTGGGA 
     3961 CAGCATTTGC CGTCAGCGTC CTGGACTCAC TCATGAGCGC GACATACTTC AATGACAATA 
     4021 TCCTCACCCT AATACGGACC CTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTAA 
     4081 TAGCTGAGGA GAATGCACTT CGAGGAGGCT ACAGCACTCC GCAGACATTG GCCAACAGGG 
     4141 ACCGTTGCCG AGTGGCCCAG TTAGCCCTGT TAGATGGTCC CTTTGCAGAC TTAGGGGATG 
     4201 GTGGTTGTTA TGGTGATCTG TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG 
     4261 GAATTTACCG GCTGAGAGAT GCCCACCTCA GCACCCCCAG CCAGTGTACA AAAAGGTACG 
     4321 TCATCACCAA TCCTCCCTAC GAGTTTGAGC TCGTACCAAC AGACCTGATC TTCTGCCTGA 
     4381 TGCAGTTTGA CCACAACGCT GGCCAATCCC GGGCCAGTCT GTCTCATTCC TCCCACTCCT 
     4441 CACAGTCGTC CAGTAAGAAG AGCTCCTCCG TCCACTCCAT CCCGTCCACA GCAAATCGGC 
     4501 CGAACCGGCC CAAGTCCAGG GAGTCCCGCG ACAAACAGAA TGCAACAAGG ATGACTAGAA 
     4561 TGGGCCAAGA AAAGAAATGG TTTACAGATG AGCCGGATAA TGCCTATCCC AGAAACATTC 
     4621 AAATCAAGCC CATGAGTACC CACATGGCTA ACCAGATCAA CCAATATAAA TCCACAAGCA 
     4681 GCCTGATCCC TCCAATCAGA GAAGTTGAAG ATGAATGTAG CGGACCGACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//