LOCUS       MR231699                8568 bp    DNA     circular  SYN 
DEFINITION  Aff3 (myc-DDK-tagged) - Mouse AF4/FMR2 family, member 3 (Aff3)
ACCESSION   NM_001290814
VERSION     NM_001290814.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4725..4816 
                     /label="Myc-DDK"  
     misc_feature    4846..5431 
                     /label="PolyA signal"  
     misc_feature    4903..4922 
                     /label="Restriction Enzyme"  
     misc_feature    5707..6379 
                     /label="ColE1"  
     misc_feature    6809..7603 
                     /label="Kan/Neo"  
     misc_feature    7638..7940 
                     /label="SV40 ori"  
     misc_feature    8026..8503 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4707..4713 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4707 
                     /label="Aff3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGCTTC GACTTAGCCC TGCTCCAGGA ATGGGACCTC GAGTCGCTGT 
     1081 GTGTCTATGA ACCGGACAGG AATGCGTTAC GGAGGAAAGA GCGAGAAAGA AGAAGTCAAG 
     1141 AAACACAGCA GGACAGTGGC AGCTTTAACT CCGGTTACTC GCTCTTCAGC GAACCCTACA 
     1201 AGACGAACAA GGGGGATGAA CTCTCCAACC GGATCCAGAA TACATTAGGA AATTATGATG 
     1261 AGATGAAAGA CTTTTTAACT GATAGATCCA ACCAGAGTCA TCTTGTTGGC GTCCCCAAGC 
     1321 CAGGTGTCCC TCAGACTCCC GTGAACAAGA TAGATGAGCA TTTTGGGGCA GAGTCGAGAG 
     1381 CCCAGCCCCA GCCCTCAACC GTCTGCAGCA CTGCGTCCTC TACACCTGCA GCTGTCCCTG 
     1441 TGCAGCAGGG CAAGAGAGGT GCCATGGGCT GGCAGAAGGC TGGGCACCCA CCGTCCGATG 
     1501 GCCAACAGAG AGCAGCGCAA CAGGGCTCTC TCAGGACCTT GCTTGGAGAT GGTGTTGGCA 
     1561 GACAGCAGAC AAGGGCCAAG CAAGTGTGCA ATATGGAGAC GGGGCTTCAG ACCCAGGAGA 
     1621 GGCCACCTGC AATGGCAGCC AAGCACGGCG GCAGTGGACA CTGTGTTCAG AACTTTCCCC 
     1681 CATCCCTGGC TTCAAAACCC AGCCTGGTGC AGCAGAAACC AACTGCCTAC GTGCGGCCAA 
     1741 TGGATGGCCA GGACCAAGCT CCTGACGAAT CTCCTAAGCT GAAGTCATCC ACAGAAACTG 
     1801 CTGTGCACTG CACAGCCTAC AGAGGGGTCC CGGCCAACAA ACCAGAGTCA GCCCGAGCCA 
     1861 AGGCCAAGCT TGCCAAGTTC AGCATCCCCA AGCAGGCAGA GGAGAGTAGA TCTGGAGAAA 
     1921 ACAACAGCTG TGTGGAAGAA ATAATTCGGG AGATGACCTG GCTTCCTCCA CTTTCTGCTA 
     1981 TTCAAGCTCC TGCCAAAGTG GAACCAAGCA AGTTCCCATT CCCAAATAAG GACTCGCAAC 
     2041 TTGTATCCTC TGGACACAGT AATCCAAAGA AAGCTGATGC GGAGCCAGGG AGTCCAGACA 
     2101 ATGGCGCATC GAACACTTCA ACTCTGGAAG ACGACCTTAA GCTAAGCAGT GATGACGAAG 
     2161 AGGGTGAGCA GCAGGCAGCC CAGAGAACAG CTCTCCGCGC TCTAGCTGAC AGCTCCGTGG 
     2221 TTCAGCAGAC CAACTGTAGA GGTTCGGCGC CATCCAGCAA GGGCGGCGGC AGCAGCAGCA 
     2281 GCAGCGGCGG CAGCAGCTCC TCCAGCGACT CAGAGAGCAC TTCCGGCTCC GACTCCGAGA 
     2341 CGGAGAGCAG CTCCAGCTCC AGCGAGAGCG AGGGCAGCAA GCCTCCGCAC TGCTCCAGCC 
     2401 CCGAGGCCGA ACCTGCGTCC TCTAACAAGT GGCAGCTGGA TAAATGGCTA AACAAAGTTA 
     2461 ATCCTCACAA GCCGCCAATT CTGATCCAAA ATGAAAGCCA CGGCCCGGAA AGGAATCAAT 
     2521 ACTACACGCC GCCGGTGAAA GATGAAGGGC AAGACTGCGG GAAACTTCCT GAGATCTGCC 
     2581 AAGCCAGCCT GAGGGACAAG GAGCTCAAGA CCACCTGCAA GGAGGAGCAG AGGCCGAGGA 
     2641 CCGCAAACAA GGCCCCTGGC AGCAAAAGTG TAAAGCAGAA GTCCCCACCC GCTGCTGTGG 
     2701 CTGTGACCGC AGCTGCCCTC CCGCCCGCGG TGCCCAGCGC ACCCACGGAG AGCGCGCCTG 
     2761 CGCCCACCCG CAGGTCAGCA GGCAAGAAGC CCACCCGGCG CACCGAGAGG ACCTCAGCCG 
     2821 GGGACGGTGC CAACTGCCAC CGGCCTGAGG AGCCAGTGGC CCCAGACACG CTGGGGGCGA 
     2881 GCGTGGTGGG ACCCCTAGAG CCCCCCAAAA CCAGACCCTG CGGTAACAAC AGGACAGGCC 
     2941 ACCGCAAGGA GCTGCGCTCC TCCGTGACCT GTGAGAAGCG GCGCACGAGG GGGCTGAGCA 
     3001 GAATCGTCCC CAAATCCAAG GAGTTTATTG AAACAGAATC TTCGTCTTCC TCTTCCTCCT 
     3061 CGGACTCAGA CCTGGAGTCA GAGCAAGAGG AGTACGTCCT GTCCAAGGCT CCGACCACCA 
     3121 CTGGGAGCGA GCAGCGGCTG AAGGAGGCCG CCAGCAGCAA CAACAACAGC AACAGCAACA 
     3181 GCAGCACCTC CCGGGCTTCT GTGGGCTCCA TCAACGCCAG GACCACCAGT GACATTGCCA 
     3241 AGGAGCTAGA GGAACAGTTC TACACGCTGG TCCCCTTTGG CAGGAATGAA CTACTATCTC 
     3301 CGCTAAAGGA CAGCGATGAG GTCAGGTCTC TCTGGGTCAA AATTGACCTG ACCCTCCTGT 
     3361 CCAGGATCCC AGAACACCTG TCCCAGGAGC CTGGGGTTTT GAGTGCTCCT TCGGCCAAGG 
     3421 ACACTGATAG TGCCCCAGCC AGTCATGCAT TAGACGCTCC TGCTGAAAAG ACTTTGCCAA 
     3481 AATCCAAGAG GAAACGCAAG TGCGACAACG AAGATGACTA CAGGGAGATA AAGAAGGTCC 
     3541 AGGGAAGGAA GGAGAGTGCC TCACGCCTCG CCGCCTCCAC TAATAACACC TTGTCCGGCA 
     3601 ATCACTGCAA TGTGAACGTC AACAGCTTGG CAATACCAAT AAACAAAAAT GAAAAGATGC 
     3661 TCCGGTCGCC CACTTCGCCA CTCTCAGACA CATGTAAACA CAAATACGCC AGCGAGGACC 
     3721 TGACTTCTTC CAGCCGACCT CACGGCAACG GGTTGTTGAC TTCAGCGTCT TCCAACAAAG 
     3781 AGCCAAAGGC TGAGAGTCAG CTGCAGACGA TTGCTGGGGA CCTCACGAAG GCATCTCACA 
     3841 ACAGTTCTGA AAATGGCACC CTCCATAGCA AGTCACGGCC CCAGACGGAG CCATGGTCTC 
     3901 CAGGTTCCAA TGGCCACAGG GACTGCAAGA GACAGAAACT GATCTTCGAT GACATGCCTC 
     3961 GAAGTGCAGA TTATTTTATG CGAGAAGCCA AGCGGATGAA ACATAAAGCA GACGCCATGG 
     4021 TGGAGAAGTT CGGGAAGGCC CTGAACTATG CCGAAGCTGC CTTGTCCTTC ATCGAGTGCG 
     4081 GAAACGCAAT GGAACAAGGG CCAATGGAAT CCAAGTCTCC CTACACCATG TACTCGGAGA 
     4141 CAGTGGAGCT CATCAGGTAT GCTATGAGAC TAAAAACCCA CTCAGGCCCC AATGCCACAC 
     4201 CAGAGGACAA ACAGTTAGCT GCGCTTTGTT ACCGATGCCT GGCTCTCCTA TACTGGCGGA 
     4261 TGTTTCGACT CAAAAGAGAC CATGCTGTAA AATATTCAAA AGCACTTATC GACTATTTCA 
     4321 AGAATTCATC GAAAGCTGCC CAAGCCCCAT CCCCATGGGG GTCCAGTGGC AAGAGCACTG 
     4381 GAAGCCCATC CCCCATGTCT CCCAACCCTT CTCCCGCCAG CTCCGTGGGG TCTCAGGGCA 
     4441 GCCTCTCCAG CTCCAGCGGC CTGTCCCCAT CGACCATCGT CAGCATCCCC CAGCGCATTC 
     4501 ACCAGATGGC GGCCAATCAC GTCAGCATCA CCAACAGCAT CCTCCACAGC TATGACTACT 
     4561 GGGAGATGGC TGATAACCTG GCCAAGGAAA ACAGAGAGTT CTTCAATGAC CTGGATTTGC 
     4621 TCATGGGACC TGTCACCCTG CACAGCAGCA TGGAACACCT GGTCCAGTAC TCCCAGCAAG 
     4681 GCCTGCATTG GCTTCGGAGC AGCGTACACC TGTCCACGCG TACGCGGCCG CTCGAGCAGA 
     4741 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4801 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4861 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4921 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4981 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5041 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5101 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5161 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5221 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5281 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5341 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5401 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5461 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5521 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5581 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5641 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5701 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5761 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5821 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5881 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5941 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6001 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6061 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6121 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6181 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6241 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6301 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6361 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6421 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6481 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6541 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6601 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6661 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6721 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6781 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6841 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6901 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6961 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7021 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7081 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7141 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7201 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7261 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7321 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7381 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7441 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7501 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7561 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7621 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7681 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7741 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7801 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7981 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8041 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8101 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8161 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8221 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8281 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8341 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8401 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8461 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8521 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//