LOCUS       MR231698                8556 bp    DNA     circular  SYN 
DEFINITION  Nfat5 (myc-DDK-tagged) - Mouse nuclear factor of activated T cells 5 (Nfat5), transcript variant c
ACCESSION   NM_001286260
VERSION     NM_001286260.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="Nfat5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCGGAC TTCATCTCAT TGCTCAGCGC GGACCTAGAC CTGGAATCGC 
     1081 CCAAGTCCCT GTACTCGCGA GAATCTGTCT ATGATCTTCT CCCAAAGGAG TTACAGTTAC 
     1141 CTCCACCTAG AGAAACATCT GTAGCATCAA TGAGTCAGAC AAGCGGTGGT GAGGCAGGCT 
     1201 CGCCTCCTCC AGCTGTAGTT GCTGCTGATG CTTCTTCAGC TCCCTCCTCT TCCTCCATGG 
     1261 GCGGTGCTTG CAGCTCCTTT ACCACCTCTT CCAGCCCTAC CATTTATTCT ACCTCAGTCA 
     1321 CCGACAGCAA GGCTATGCAA GTGGAGAGCT GCTCCTCAGC CGTGGGGGTA AGTAACAGAG 
     1381 GGGTAAGTGA AAAGCAGTTA ACCGGTAACA CAGTTCAGCA GCATCCATCA ACCCCGAAGA 
     1441 GGCACACAGT TTTGTACATC TCACCACCAC CTGAGGACTT GCTGGATAAC AGTCGGATGT 
     1501 CCTGCCAGGA TGAGGGGTGT GGATTGGAAT CTGAGCAGAG CTGCAGTATG TGGATGGAGG 
     1561 ATTCCCCCTC CAACTTCAGT AACATGAGCA CCAGTTCCTA CAATGATAAC ACTGAGGTAC 
     1621 CTCGTAAATC ACGAAAACGA AATCCAAAGC AGAGGCCGGG GGTCAAACGA CGAGATTGTG 
     1681 AAGAATCTAA TATGGATATA TTTGATGCCG ACAGTGCCAA AGCACCTCAC TATGTGCTTT 
     1741 CTCAGCTTAC CACGGACAAC AAAGGCAACT CAAAAGCTGG AAATGGAACA TTGGACAGCC 
     1801 AAAAGGGAAC TGGAGTAAAG AAGAGCCCTA TGTTGTGCGG ACAGTATCCG GTTAAAAGTG 
     1861 AGGGGAAGGA GCTGAAGATA GTGGTACAGC CTGAAACCCA ACACCGAGCC CGGTACCTGA 
     1921 CAGAGGGCAG CCGAGGCTCT GTAAAAGACA GAACACAGCA AGGCTTTCCT ACGGTGAAGC 
     1981 TGGAAGGCCA TAATGAACCA GTGGTGTTGC AGGTATTTGT GGGCAATGAT TCTGGTCGAG 
     2041 TAAAACCACA TGGATTCTAT CAGGCCTGTA GAGTAACTGG GCGAAATACA ACTCCCTGCA 
     2101 AAGAAGTGGA CATTGAAGGT ACCACTGTTA TAGAAGTTGG TCTTGATCCT AGCAACAACA 
     2161 TGACACTGGC GGTGGACTGT GTTGGAATAT TGAAGCTGAG GAATGCCGAT GTTGAAGCCA 
     2221 GAATTGGAAT TGCTGGATCT AAGAAAAAGA GCACTCGTGC CAGATTGGTT TTTCGAGTTA 
     2281 ATATCACAAG GAAAGATGGC TCTACTCTGA CATTGCAGAC ACCTTCTTCC CCCATTTTAT 
     2341 GCACTCAGCC AGCAGGAGTT CCTGAGATCT TAAAGAAAAG CTTGCATAGC TGTTCAGTGA 
     2401 AAGGAGAGGA AGAAGTATTT TTAATTGGGA AAAACTTTCT GAAAGGAACT AAAGTTATTT 
     2461 TCCAGGAAAA TGTTTCTGAT GAAAACTCTT GGAAATCAGA AGCTGAAATT GACATGGAGT 
     2521 TATTCCATCA GAACCATCTT ATTGTGAAGG TTCCTCCGTA TCATGACCAA CATATAACTT 
     2581 TGCCTGTATC AGTGGGAATA TATGTTGTGA CCAATGCTGG AAGATCTCAT GATGTTCAGC 
     2641 CATTTACGTA CACTCCGGAT CCAGCAGCTG GTGCTTTGAA TGTAAATGTG AAAAAGGAGA 
     2701 TATCTAGTCC AGCAAGACCT TGCTCTTTTG AAGAAGCCAT GAAAGCAATG AAAACGACTG 
     2761 GATGTAACGT AGATAAGGTG ACTATCCTTC CTAATGCCCT GATCACTCCA CTCATATCAA 
     2821 GCAGTATGAT TAAGACTGAA GATGTTACTC CAATGGAAGT AACTTCAGAA AAAAGATCTT 
     2881 CCCCAATTTT TCAGACTACA AAGAGCATTG GATCAACCCA GCAAACCTTA GAAACTATTT 
     2941 CTAACATAGC GGGGGGTGCA CCCTTCTCCT CACCGTCGTC ATCTTCCCAC TTAACTCCTG 
     3001 AAAGTGAGAA TCAGCAGCAG CTTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA 
     3061 TCCAGACACA GGACATCTCA CAGCCCGGGA CCTTTCCCGC AGTTTCTGCT GCTAGTCAGC 
     3121 TGCCCAGCAG CGATGCACTA CTGCAGCAGG CAACACAGTT TCAGACAAGA GAAGCTCAGT 
     3181 CCAGAGACAC AATACAGTCA GATACAGTGG TTAACTTGTC ACAGTTAACT GAAGCATCAC 
     3241 AGCAGCAGCA ATCCCCACTA CAAGAACAAG CACAAACGTT ACAGCAGCAG ATACCATCAA 
     3301 ATATTTTTCC ATCGCCAAGC AGTGTCAGCC AGCTCCAGAG TACTATTCAG CAGCTGCAAG 
     3361 CAGGAAGTTT CACAGGCAGC GCTGCTGGCG GCAGGAGTGG AAGCGTTGAT TTGGTCCAGC 
     3421 AGGTTTTAGA GGCACAACAG CAGTTATCGT CTGTTCTGTT TTCTACTCCA GATGGGAATG 
     3481 AGAATGTTCA AGAACAGCTT AATGCAGACA TTTTCCAAGT CAGTCAAATT CAAAATAGTG 
     3541 TAAGCCCTGG AATGTTTTCT TCAGCGGAGT CTGCAGTTCA CACTAGACCA GATAACTTAC 
     3601 TACCTGGGAG GGCTGACAGC GTCCATCAAC AGACTGAAAA TACACTGTCT AATCAGCAGC 
     3661 AGCAGCAACA GCAGCAGCAG CAAGTGATGG AGTCATCAGC TGCAATGGTG ATGGAGATGC 
     3721 AGCAGAGCAT TTGCCAAGCA GCTGCCCAGA TCCAGTCAGA GCTGTTTCCT TCAGCTGCTT 
     3781 CAGCAAGTGG AAGCCTTCAG CAGTCTCCAG TTTACCAGCA GCCTTCTCAC ATGATGAGTG 
     3841 CACTGCCTAC CAACGAGGAC ATGCAGATGC AGTGTGAGCT GTTCTCGTCT CCCCCTGCAG 
     3901 CTTCTGGAAA CGAAACTTCC ACAACCACCA CGCCACAGGT GGCAACCCCT GGCTCCACCA 
     3961 TGTTTCAGAC ACCAAGTTCA GGAGATGGAG AAGAAACTGG AGCACAAGCA AAACAGATTC 
     4021 AGAACAGCGT CTTTCAGACA ATGGTCCAAA TGCAGCGCAG TGGTGACAGC CAACCTCAAG 
     4081 TAAACCTCTT TTCATCTACA AAAAATATAA TGAGTGTTCA GAATAATGGT ACCCAGCAAC 
     4141 AAGGGAATAG CTTGTTCCAG CAAGGGAGCG AGATGATGTC ACTTCAGTCT GGGAACTTTT 
     4201 TGCAGCAGTC CTCTCATTCA CAGGCTCAGC TCTTTCATCC TCAAAATCCT ATTGCTGATG 
     4261 CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CAATTTTTCA TAGTCCAAAT CCTATTGTCC 
     4321 ACAGTCAAAC TTCCACAGCA TCCTCTGAAC AACTGCAGCC TTCAATGTTT CACTCTCAGA 
     4381 ATACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA CCCAACATAT 
     4441 TTCTTTCCCA AAGTTCTATC AATAATCTTC AAACTAACAC AGTAGCCCAA GAAGAGCAGA 
     4501 TTTCATTTTT TGCAGCACAG AACTCAATTT CTCCACTTCA GTCAACATCA AACACTGAGC 
     4561 AGCAAGCTGC TTTCCAACAG CAGCCTCCAA TCTCACACAT CCAGACCCCT ATTCTTTCCC 
     4621 AGGAACAGGC ACAACCCTCT CAGCAAGGGT TATTTCAGCC TCAGGAAAGC TTACACTCAC 
     4681 ACATAACCCC AGACGCTTGC AAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//