LOCUS       MR231697                8553 bp    DNA     circular  SYN 
DEFINITION  Kcnt1 (myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3
ACCESSION   NM_001302351
VERSION     NM_001302351.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4710..4801 
                     /label="Myc-DDK"  
     misc_feature    4831..5416 
                     /label="PolyA signal"  
     misc_feature    4888..4907 
                     /label="Restriction Enzyme"  
     misc_feature    5692..6364 
                     /label="ColE1"  
     misc_feature    6794..7588 
                     /label="Kan/Neo"  
     misc_feature    7623..7925 
                     /label="SV40 ori"  
     misc_feature    8011..8488 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4692..4698 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4692 
                     /label="Kcnt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGGGCC AAGCTGCCGC GCTCGCCGTC CGAGGGCAAG GCGGGTCCGG 
     1081 GGGACACCCC AGCCGGCGCT GCAGCCCCTG AGGAGCCACA CGGGCTCAGC CCGCTACTGC 
     1141 CGGCCCGCGG CGGGGGCTCC GTGGGCAGCG ACGTGGGCCA GAGGGTCCAG GTGGAGTTCT 
     1201 ATGTCAATGA GAACACCTTC AAAGAGCGGC TCAAGCTGTT CTTCATCAAA AACCAAAGAT 
     1261 CCAGCCTGAG GATCCGGCTG TTCAACTTCT CCCTCAAGCT CCTCACCTGC CTGCTGTATA 
     1321 TCGTCCGTGT CCTGCTTGAC AACCCAGACC AGGGCATCGG ATGCTGGGGC TGCACGAAGT 
     1381 ATAACTACAC TTTCAATGGC TCATCCTCTG AGTTCCACTG GGCTCCCATC CTGTGGGTGG 
     1441 AGAGGAAAAT GGCTCTGTGG GTGATCCAGG TCATTGTGGC CACAATAAGC TTCTTAGAGA 
     1501 CCATGCTCAT CATTTACCTC AGCTACAAAG GCAACATCTG GGAGCAGATA TTCCATGTGT 
     1561 CTTTCGTCTT GGAGATGATC AACACACTGC CCTTCATCAT CACGGTCTTC TGGCCACCTC 
     1621 TGCGGAACCT GTTCATCCCC GTGTTTCTCA ACTGCTGGCT GGCCAAGCAT GCGCTGGAGA 
     1681 ACATGATTAA TGACTTCCAC CGTGCCATCC TACGCACACA GTCAGCCATG TTCAACCAGG 
     1741 TGCTCATCCT GTTCTGCACC CTGCTGTGCC TGGTCTTCAC AGGGACCTGT GGGATCCAGC 
     1801 ACCTGGAGCG AGCAGGTGGC AACTTGAACC TGCTGACCTC CTTCTACTTC TGCATCGTGA 
     1861 CTTTCTCAAC TGTGGGCTTC GGTGATGTGA CGCCCAAGAT CTGGCCATCC CAGCTCCTGG 
     1921 TGGTCATCCT GATCTGTGTC ACCCTTGTGG TGCTCCCACT GCAATTTGAA GAGCTTGTCT 
     1981 ACCTCTGGAT GGAGCGTCAG AAGTCAGGGG GCAACTATAG CCGCCACCGA GCACGGACGG 
     2041 AGAAGCACGT AGTCCTGTGT GTGAGCTCCC TCAAGATTGA TCTCCTCATG GACTTCCTGA 
     2101 ATGAGTTCTA TGCCCATCCC CGGCTCCAGG ACTACTACGT GGTCATCCTG TGTCCCTCTG 
     2161 AAATGGACGT CCAGGTGCGC AGGGTGCTGC AGATTCCCCT GTGGTCCCAG CGGGTCATCT 
     2221 ACCTCCAGGG CTCTGCCCTC AAGGACCAGG ATCTCATGCG AGCCAAGATG GACAACGGAG 
     2281 AGGCCTGCTT TATCCTCAGC AGCAGGAATG AGGTGGACCG CACAGCTGCG GATCACCAGA 
     2341 CCATCCTTCG AGCCTGGGCT GTGAAGGACT TTGCCCCCAA CTGTCCCCTC TATGTCCAGA 
     2401 TCCTCAAGCC CGAAAACAAG TTTCACGTCA AATTTGCTGA CCACGTGGTA TGCGAGGAAG 
     2461 AGTGCAAGTA CGCCATGCTG GCCCTGAACT GCATCTGCCC GGCCACCTCC ACCCTCATCA 
     2521 CCCTGCTGGT GCACACGTCC CGTGGCCAGG AAGGACAGGA GTCTCCCGAG CAGTGGCAGC 
     2581 GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT GACAGTAAAT 
     2641 TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG CACAAGAAAT 
     2701 ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG CTGAACCCAG 
     2761 GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT ACCAAGGAGG 
     2821 AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC CTTGCAGGGC 
     2881 AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC TCTATGGGGA 
     2941 CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC AGTGGCGGGG 
     3001 ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC CCCAGCATCG 
     3061 CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG CTGAGTGACC 
     3121 AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT GAGTACGTGA 
     3181 AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC CACCTCCTGC 
     3241 CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC AACAGCTACG 
     3301 AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT GAGACGGCAG 
     3361 GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC CGCCGGGAGC 
     3421 TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG GAGGCCATCT 
     3481 GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC AGCTTACTGC 
     3541 AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC ACTATGAGCG 
     3601 CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC ATGTTCCGGC 
     3661 TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG CGGTTCATGC 
     3721 AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG CAAGAACGGG 
     3781 AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT CGAGTATTTA 
     3841 GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC ATGATCACCA 
     3901 TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC TGTGCAATGA 
     3961 AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG AAACTCTGCT 
     4021 CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC TTCTCGGAGC 
     4081 CCCATGACGT CAGAGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC GAGGATACTC 
     4141 GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC ACCCATGGCC 
     4201 GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG AGCCTGCAGT 
     4261 GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT GTGGCCACAG 
     4321 ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG GAGCTCTCAG 
     4381 AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT GAGGACGTAG 
     4441 CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG CAAGATGAGA 
     4501 TGAACGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG CCAGACACAA 
     4561 GACTGGAACC CAACGACATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC CATGTGGCCA 
     4621 GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAGCT CTCATCCTGT AATCCTGAGA 
     4681 CCAGGGATGA GACCCAGCTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4741 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4801 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4861 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4921 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4981 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5041 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5101 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5161 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5221 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5281 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5341 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5401 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5461 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5521 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5581 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6181 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6241 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6301 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6361 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6421 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6481 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6541 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6601 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6661 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6721 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6781 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6841 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6901 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6961 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7021 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7081 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7141 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7201 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7261 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7321 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7381 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7441 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7501 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7561 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7621 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7681 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7741 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7801 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7981 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8041 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8101 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8161 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8221 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8281 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8341 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8401 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8461 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8521 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//