LOCUS       MR231694                8538 bp    DNA     circular  SYN 
DEFINITION  Adcy8 (myc-DDK-tagged) - Mouse adenylate cyclase 8 (Adcy8), transcript variant 2
ACCESSION   NM_001291903
VERSION     NM_001291903.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="Adcy8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTCG GATGTGCACT GCCTTAGTGG CAGCGAGGAA CTCTACACCA 
     1081 TCCAACCGAC GCCCCCGGCC GGCGACGACG GGAGCGGCTC TCGGCCGCAG CGGCTGCTGT 
     1141 GGCAGACGGC GGTGCGGCAC ATCACGGAGC AGCGCTTCAT CCACGGGCAC CGAGGCGGCG 
     1201 GCGGCGGCGG GGTCTCCCGC AAAGCCTCGA ACCCTGCGGG CAGTGGACCC AATCACCACG 
     1261 CGCCGCAGCT GTCTAGCGAC TCGGTGCTGC CTCTCTATTC TCTGGGCCCC GGAGAGCGAG 
     1321 CGCACAACAC CGGTGGCACC AAAGTCTTTC CGGAACGCAG CGGGAGCGGC AGTGCCAGTG 
     1381 GCAGCGGGGG CGGGGGCGAC TTGGGCTTCC TACACCTTGA CTGTGCCCCA AGTAACTCGG 
     1441 ATTTCTTCCT CAATGGAGGA TACAGCTACC GAGGGGTCAT TTTTCCAACC CTACGCAACT 
     1501 CCTTCAAGTC TCGGGATCTG GAGCGCCTCT ACCAACGCTA TTTCCTGGGC CAGAGGCGCA 
     1561 AATCGGAGGT AGTGATGAAC GTGTTGGATG TACTAACCAA ACTCACCCTT TTAGTCCTGC 
     1621 ACTTGAGCCT GGCCTCGGCT CCAATGGACC CTCTCAAGGG CATCCTGCTA GGCTTTTTCA 
     1681 CTGGCATCGA GGTGGTGATC TGCGCCCTCG TGGTGGTCAG GAAGGACAAC ACCTCCCACA 
     1741 CTTACCTGCA ATACAGCGGC GTGGTCACTT GGGTGGCTAT GACCACCCAG ATTCTGGCAG 
     1801 CAGGCCTGGG CTATGGGCTT CTGGGCGACG GCATAGGCTA CGTGCTTTTT ACACTCTTCG 
     1861 CCACCTACAG CATGCTTCCC CTGCCTCTCA CCTGGGCCAT CTTGGCCGGC CTGGGCACAT 
     1921 CTTTGCTGCA AGTCACCCTT CAAGTACTCA TACCCAGGCT AGCGGTCTTT TCCATCAACC 
     1981 AGGTCCTGGC CCAGGTGGTG CTATTCATGT GTATGAATAC AGCAGGAATC TTCATCAGTT 
     2041 ACCTTTCAGA CCGTGCCCAG CGCCAGGCCT TCCTGGAGAC CCGGAGGTGT GTGGAGGCCA 
     2101 GGCTCCGCTT GGAGACAGAG AACCAAAGAC AGGAGCGGCT TGTGCTGTCT GTGCTCCCCA 
     2161 GGTTTGTCGT CCTGGAAATG ATCAACGACA TGACCAATGT GGAGGATGAG CACCTGCAGC 
     2221 ATCAGTTCCA CCGCATCTAC ATCCATCGCT ATGAGAACGT CAGTATTCTT TTTGCAGATG 
     2281 TCAAAGGATT TACCAACCTC TCTACGACCT TGTCTGCTCA GGAGCTGGTC AGGATGCTCA 
     2341 ACGAACTCTT TGCCAGATTC GACCGGCTGG CCCATGAGCA TCACTGTCTT CGCATTAAAA 
     2401 TCCTGGGGGA CTGCTACTAC TGTGTGTCTG GACTGCCTGA GCCCCGCCGG GACCACGCTC 
     2461 ATTGCTGTGT TGAAATGGGC CTCAGCATGA TCAAAACTAT CAGGTTTGTG AGATCCAGAA 
     2521 CGAAGCACGA TGTTGACATG CGAATTGGAA TCCATTCAGG TTCTGTGCTA TGTGGTGTGT 
     2581 TGGGCCTGAG GAAATGGCAG TTTGATGTCT GGTCTTGGGA TGTGGACATA GCAAACAAGC 
     2641 TTGAGTCTGG AGGAATCCCT GGGAGGATTC ACATTTCTAA AGCCACCCTG GATTGCCTCA 
     2701 ATGGTGACTA TAACGTGGAA GAGGGTCACG GGAAAGAGAG GAACGAATTC TTGAGGAAGC 
     2761 ATAACATAGA GACCTATTTG ATTAAGCAGC CTGAGGAGAG TTTGCTCTGC TTGCCTGAAG 
     2821 ACATCGTTAA GGAGTCGGTG AGCTGCTCTG ACAGGAGAAA CAGTGGAGCA ACATTCACAG 
     2881 AAGGATCGTG GAGCCCAGAG CTGCCTTTCG ACAACATCGT GGGCAAACAG AATACTCTGG 
     2941 CTGCCCTAAC AAGAAATTCA ATAAATCTGC TTCCAAACCA TCTCGCACAA GCTTTGCATG 
     3001 TCCAGTCTGG GCCTGAGGAA ATTAACAAGA GAATAGAGCA TACCATCGAC TTGCGGAGTG 
     3061 GCGATAAGTT GAGAAGAGAG CATATCAAGC CATTCTCACT GATGTTTAAA GACTCCAGCC 
     3121 TGGAGCACAA GTATTCTCAA ATGAGGGATG AAGTATTCAA GTCAAACTTG GTCTGTGCAT 
     3181 TTATCGTTCT TCTGTTTATC ACTGCAATTC AAAGTTTGCT TCCTTCTTCA AGGCTGATGC 
     3241 CTATGACTAT CCAGTTCTCC ATCCTGATCA TGCTGCACTC TGCCCTGGTC CTCATCACCA 
     3301 CAGCAGAAGA CTATAAGTGT CTGCCCCTCA TCCTCCGAAA AACCTGCTGT TGGATTAATG 
     3361 AGACCTATCT GGCCCGCAAC GTCATCATCT TCGCTTCCAT CTTAATTAAC TTCCTGGGAG 
     3421 CTGTCCTAAA TATTTACTTT GTCTTCACTG GGGTGTTGGC CATGGTGACC TGTGCTGTGT 
     3481 TTCTACGGCT TAACTCTGTC CTGAAGCTGG CAGTGCTACT CATCATGATC GCCATCTACG 
     3541 CCCTGCTCAC AGAGACCATC TACGCAGGTC TCTTTCTGAG TTATGACAAC CTGAACCACA 
     3601 GTGGAGAAGA TTTCCTGGGG ACCAAGGAAG CATCACTGCT GCTGATGGCC ATGTTCCTTC 
     3661 TTGCTGTGTT CTACCATGGA CAACAGCTGG AGTACACAGC CCGCCTGGAT TTCCTGTGGC 
     3721 GCGTACAGGC CAAAGAGGAG ATCAACGAGA TGAAGGAACT GAGGGAACAC AACGAGAACA 
     3781 TGCTGCGCAA TATCTTACCC AGCCATGTGG CCCGCCACTT CCTGGAGAAA GACAGAGACA 
     3841 ATGAGGAGCT GTATTCTCAA TCCTACGATG CTGTTGGAGT GATGTTCGCC TCCATCCCTG 
     3901 GGTTCGCAGA CTTCTACTCT CAGACAGAAA TGAACAACCA GGGAGTAGAA TGTCTGCGCT 
     3961 TGCTGAATGA GATCATTGCT GACTTTGATG AGCTGCTCGG GGAGGACCGG TTTCAGGACA 
     4021 TTGAGAAGAT TAAGACCATT GGTAGTACAT ACATGGCTGT CTCAGGACTG TCCCCAGAGA 
     4081 AACAGCAATG TGAAGATAAA TGGGGACATT TGTGTGCCCT GGCTGACTTC TCTCTTGCTC 
     4141 TGACTGAAAG CATACAAGAG ATCAACAAGC ATTCATTCAA CAATTTTGAA CTCCGAATCG 
     4201 GCATCAGCCA TGGCTCAGTG GTGGCAGGTG TAATTGGAGC TAAGAAACCA CAGTATGACA 
     4261 TTTGGGGGAA AACTGTGAAC TTGGCAAGCC GAATGGACAG CACGGGAGTA AGTGGCCGGA 
     4321 TCCAAGTTCC TGAGGAGACC TACCTTATCC TGAAGGACCA GGGCTTTGCC TTTGACTACC 
     4381 GTGGGGAGAT ATATGTGAAG GGCATCAGTG AACAAGAAGG GAAAATCAAA ACATACTTTC 
     4441 TCCTGGGACG AGTCCAACCC AACCCATTCA TCTTACCCCC AAGGAGACTT CCCGGGCAAT 
     4501 ACTCTCTGGC TGCGGTTGTC CTTGGCCTTG TCCAGTCTCT CAACAGGCAA AGGCAGAAGC 
     4561 AACTTCTCAA CGAGAACAGC AATTCGGGCA TCATCAAGAG CCATTACAAC CGGCGGACTT 
     4621 TGCTAACGCC AAGTGGGCCA GAGCCTGGAG CACAAGCTGA AGGCACTGAC AAATCCGATT 
     4681 TGCCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//