LOCUS       MR231691                8523 bp    DNA     circular  SYN 
DEFINITION  Sorbs2 (myc-DDK-tagged) - Mouse sorbin and SH3 domain containing 2 (Sorbs2), transcript variant 1
ACCESSION   NM_001205219
VERSION     NM_001205219.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="Sorbs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATACAGAT AGCGGTGGGT GTGCTCGCAA ACGTGCCGCC ATGTCTGTTA 
     1081 CGTTAACATC TGTGAAGAGA GTTCAAAGTT CTCCAAACCT ATTGGCTGCA GGGCGTGAGT 
     1141 CTCAGTCTCC AGATTCAGCT TGGAGATCTT ACAATGATCG GAATCCAGAG ACACTGAACG 
     1201 GAGATGCCAC ATATTCCTCT CTTGCAGCAA AAGGTTTTAG AAGCGTTCGG CCAAACCTGC 
     1261 AAGACAAAAG ATCACCGACC CAGAGCCAGA TCACTATCAA TGGCAACTCT GGTGGCGCCG 
     1321 TGAGTCCAGT GAGTTACTAT CAGAGGCCAT TCTCCCCTTC TGCATACTCC CTCCCAGCCT 
     1381 CGCTGAACTC CAGCATTATC ATGCAGCACG GCAGGTCTCT TGATTCTGCA GAGACATATT 
     1441 CCCAGCATGC CCAGTCCCTG GATGGCACCA TGGGAAGCTC CATCCCGCTC TATAGATCCT 
     1501 CCGAGGAAGA GAAGAGGGTC ACGGTCATCA AAGCCCCGCA TTACCCAGGG ATCGGCCCTG 
     1561 TGGATGAGTC TGGAATCCCC ACAGCCATTA GGACGACTGT CGACAGGCCA AAGGACTGGT 
     1621 ACAAGACAAT GTTCAAGCAA ATCCACATGG TACACAAGCC GGATGAGGAC ACAGACATGT 
     1681 ATAATACTCC TTACACATAC AACGCAGGTC TGTACAACTC GCCCTACAGT GCTCAGTCCC 
     1741 ACCCGGCTGC GAAGACTCAG ACCTACAGAC CTCTTTCGAA AAGCCACTCA GACAATGGCA 
     1801 CCGATGCGTT TAAGGAGGTG CCCTCCCCAG TGCCTCCCCC ACACGTCCCA CCACGACCAA 
     1861 GAGATCAGTC TTCAACACTA AAGCATGACT GGGACCCTCC AGACAGAAAG GTGGACACCA 
     1921 GGAAATTTCG ATCGGAGCCA AGGAGTATTT TTGAATATGA GCCTGGGAAG TCGTCCATTC 
     1981 TGCAGCACGA AAGACCCCCT CCTAAAAAGG CTCTGGATTA TGTTCAAGAT CATTCTTCCG 
     2041 GTGTGTCCAA CGAGGTCTCC ATCTATCAGT CCTCCATAGA CAGAAGCCTG GAAAGACCCA 
     2101 GCAGCTCTGC AAGCATGGCC GGTGACTTTA GGAAACGGAG GAAGAGCGAA CCTGCAGTGG 
     2161 GCCCACTCCG GGGCTTGGGG GATCAGAGTT CAAGCAGGAC CAGCCCTGGC CGGGCGGACC 
     2221 TCCCAGGATC AAGTTCCACC TTTACGAAGT CTTTCATTAG CTCTTCTCCT TCCTCTCCCT 
     2281 CGAGAGCACA AGGTGGGGAT GATAGCAAAA TGTGTCCGCC CCTTTGCAGT TACTCGGGGC 
     2341 TCAATGGCAC CCCCTCTGGT GAGTTAGAGT GCTGCAACGC TTACAGACAA CATTTGGACG 
     2401 TCCCCGGGGA CTCGCAAAGG GCCATCACTT TCAAGAATGG CTGGCAAATG GCCCGGCAAA 
     2461 ATGCAGAGAT CTGGAGTAGC ACTGAAGAGA CCGTTTCCCC CAAAATCAAA TCACGTAGCT 
     2521 GCGATGATCT CCTGAATGAC GACTGCGACA GCTTCCCAGA CCCTAAAACC AAGTCAGAGA 
     2581 GTATGGGTTC TCTGTTATGT GAAGAAGACT CCAAAGAGAG CTGCCCCATG ACATGGGCGT 
     2641 CCCCCTACAT CCAGGAAGTG TGCGGGAACA GCAGATCTAG GCTCAAACAC AGGTCAGCCC 
     2701 ATAATGCCCC AGGCTTCCTC AAAATGTACA AGAAAATGCA TCGCATCAAC CGCAAGGACT 
     2761 TGATGAATTC AGAGGTCATT TGCTCGGTGA AATCCAGGAT CCTGCAGTAC GAGAAGGAGC 
     2821 AACAGCACAG GGGGCTGCTG CACGGATGGA GCCAGTCTTC CACCGAGGAG GTGCCCAGGG 
     2881 ACGTGGTGCC CACTCGCATT TCGGAATTTG AAAAGCTGAT TCAGAAGTCG AAGTCTATGC 
     2941 CCAATCTAGG AGATGAAATG TTATCTCCTA TAACCCTAGA ACCCCCACAA AATGGTTTGT 
     3001 GTCCCAAGAG GCGATTTTCT ATCGAGTCTC TGCTGGAGGA AGAAACTCAG GTTCGTCACC 
     3061 CTTCTCAGGG TCAGCGAAGC TGCAAGTCTA ACACCCTGGT GCCCATCCAC ATCGAGGTCA 
     3121 CCAGCGACGA GCAACCTAGA ACACACATGG AGTTTTCCGA CAGTGACCAA GATGGGGTTG 
     3181 TGTCCGACCA CAGCGACTAC GTTCACGTCG AAGGGTCGTC CTTTTGTAGT GAAAGTGACT 
     3241 TTGACCACTT TTCATTCACA TCCTCTGAAA GCTTCTACGG ATCGAGCCAT CACCACCACC 
     3301 ATCACCATCA CCATCACCGA CACCTCATCA GCTCCTGCAA AGGCCGATGC CCGGCTTCTT 
     3361 ACACTCGATT TACCACAATG CTAAAACATG AAAGAGCTAA GCATGAAAAC ATGGACCGAC 
     3421 CCAGAAGGCA AGAAATGGAT CCTGGCCTGT CTAAGCTTGC ATTTCTAGTC AGCCCTGTGC 
     3481 CTTTCCGAAG GAAAAAAATC CTGACTCCCC AAAAACAGAC TGAAAAGGCA AAATGCAAAG 
     3541 CCTCAGTAGT TGAAGCTCTG GACTCTGCCC TGAAAGACAT TTGCGACCAA ATAAAAGCCG 
     3601 AAAAGCGGAG GGGAAGCTTG CCAGACAACA GCATCCTGCA CAGGCTTATC AGTGAACTGC 
     3661 TGCCACAGAT TCCTGAAAGG AATTCATCTC TTCATGCTCT CAAAAGGAGC CCCATGCACC 
     3721 AGCCTTTCCA CCCACTGCCT CCAGATGGTG CTAGTCATTG TCCCCTGTAC CAAAATGATT 
     3781 GTGGGAGAAT GCCTCACAGT GCCTCTTTCC CTGACGTGGA CACAACCAGC AACTACCACG 
     3841 CACAGGACTA TGGGAGTGCA CTGAGCCTCC AAGATCACGA GTCCCCTAGA AGTTACTCAT 
     3901 CTACTCTGAC TGACTTGGGC AGAAGTGCAT CACGGGAACG AAGGGGAACT CCAGAAAAAG 
     3961 AGAAATTGCC TGCAAAAGCT GTCTATGATT TCAAAGCTCA GACATCTAAG GAGCTGTCAT 
     4021 TTAAGAAAGG AGACACTGTC TACATCCTCA GGAAAATTGA CCAAAACTGG TATGAGGGGG 
     4081 AACACCACGG CAGAGTGGGC ATTTTCCCAA TCTCATACGT AGAGAAACTA ACACCCCCGG 
     4141 AAAAAGCGCA GCCCGCAAGA CCACCACCCC CAGTCCAGCC CGGAGAGATT GGAGAAGCCA 
     4201 TAGCCAAGTA CAACTTCAAT GCAGACACAA ATGTGGAACT CTCCCTGCGA AAGGGCGACA 
     4261 GGATTATTCT TCTTAAAAGA GTTGACCAAA ACTGGTACGA AGGTAAAATC CCAGGAACCA 
     4321 ACAGACAAGG CATCTTCCCC GTGTCCTATG TCGAGGTTGT CAAGAGGAAC GCAAAAGGTG 
     4381 CTGAGGACTA CCCAGACCCT CCTCTGCCCC ACAGCTACTC CAGTGACAGA ATTTACACCC 
     4441 TAAGCTCCAA TAAGCCACAG CGTCCTGGGT TCTCTCATGA AAACATTCAA GGTGGGGGAG 
     4501 AACCGTTTCA GGCTCTGTAT AACTATACTC CTAGGAACGA AGATGAGCTG GAACTCAGAG 
     4561 AAAGTGATGT CGTTGATGTC ATGGAAAAGT GTGACGACGG ATGGTTTGTG GGAACTTCAA 
     4621 GAAGAACCAA ATTCTTTGGT ACTTTTCCCG GAAACTATGT CAAAAGGCTG ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//